Genetic organization of the transfer region of F-like plasmid pAP18-1

Department of Biology and General Genetics, Patrice Lumumba Peoples' Friendship University, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR T. T. Berezov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 110, No. 9, pp. 298–301, September, 1990.     

Novel and uncommon antimicrobial resistance genes in livestock-associated methicillin-resistant Staphylococcus aureus

Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) isolates have been the subject of numerous studies during recent years. The characterization of such isolates has usually also included the determination of their resistance phenotypes and associated resistance genotypes. Analysis of the resistance genes present in LA-MRSA isolates has revealed a number of genes commonly found in S. aureus and coagulase-negative staphylococci of humans and animals. In addition, novel resistance genes and/or resistance genes that have been rarely detected in staphylococci so far have been encountered. These include the phenicol exporter gene fexA, the multiresistance gene cfr, the tetracycline resistance gene tet(L), the trimethoprim resistance gene dfrK, the macrolide–lincosamide–streptogramin B resistance gene erm(T), the lincosamide–streptogramin A–pleuromutilin resistance genes vga(C) and vga(E), and the apramycin resistance gene apmA. Most of these genes were located on multiresistance plasmids in LA-MRSA. The co-localization of these resistance genes with other resistance genes enables their co-selection and persistence. LA-MRSA can therefore act as a donor and a recipient of antimicrobial resistance genes within the Gram-positive gene pool.     

外源表达Nkx2-5基因诱导P19细胞向心肌分化

目的:构建人Nkx2-5基因融合绿色荧光蛋白真核表达质粒pEGFP-N1-Nkx2-5,探讨外源性Nkx2-5基因诱导P19细胞向心肌分化的作用。方法:以真核表达质粒pEFSA-HA-Nkx2-5为模板,PCR扩增得到人Nkx2-5基因,将目的片段亚克隆到pEGFP-N1载体,鉴定正确。将重组质粒pEGFP-N1-Nkx2-5以脂质体法转染P19细胞,G418筛选2周,聚集4d,贴壁培养16d。以免疫细胞化学检测desmin、α-sarcomeric actin和cardiac Troponin T(cTnT)的表达。结果:将Nkx2-5基因正确插入pEGFP-N1载体,外源表达Nkx2-5基因可使P19细胞在无诱导剂、聚集条件下表达desmin、α-sarcomeric actin和cTnT蛋白。结论:成功构建真核表达质粒pEGFP-N1-Nkx2-5,外源表达Nkx2-5基因诱导P19细胞向心肌分化。     

Effect of anticonvulsant drugs on interleukins-1, -2 and -6 and monocyte chemoattractant protein-1

In order to evaluate whether treatment with valproic acid or carbamazepine can modify interleukins and monocyte chemoattractant protein-1, we studied 40 epileptic children and adolescents. We evaluated the patients before and after 1 year of therapy. At the end of follow-up, the patients showed a significant increase of the production of interleukin-1α, interleukin-1β, interleukin-6, and monocyte chemoattractant protein-1; interleukin-2 production was significantly higher only in patients receiving carbamazepine. In conclusion, antiepileptic drugs can influence the immune system by modifying interleukin and chemokine concentrations; these changes seem to be independent of the serum concentrations of these drugs. Received: 16 February 2001 / Accepted: 18 September 2001     

fin K,fin L,fin M,and fin N genetic plasmid transfer regulatory systems

Department of Biology and General Genetics, Russian University for Peoples' Friendship, Moscow. (Presented by Academician of the Russian Academy of Medical Sciences T. T. Berezov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 114, No. 8, pp. 199–201, August, 1992.