抗李斯特菌免疫机理研究进展

单核细胞增多性李斯特菌(Listeria monocytogenes)为革兰氏阳性兼性厌氧杆菌,可引起人和动物的李斯特菌病,以脑膜炎和败血症为主要临床表现,多发于新生儿、孕妇、老年人和免疫功能低下者;在动物,本病主要发生于牛和羊。李斯特菌侵入机体后可以在专职和非专职吞噬细胞中生存和繁殖,诱导以CD8^ 和CD4^ T细胞介导的特异性免疫应答;     

Listeria monocytogenes osteomyelitis

Listeria monocytogenes is usually an opportunistic pathogen causing either meningitis or bacteremia in adults. Focal infection outside the central nervous system occurs infrequently. We describe two cases of osteomyelitis caused by L monocytogenes. Certain characteristics of L monocytogenes may make cure difficult, particularly in a deep-seated focus such as bone, and may warrant special consideration when planning therapy.     

Listeria monocytogenes cholecystitis

Listeriosis usually manifests at the extremes of age, during pregnancy or among immunocompromised individuals as an acute meningoencephalitis with or without associated septicemia. Localized infections are rare. We investigated the incidence of Listeria monocytogenes cholecystitis using the data of the Listeria reference laboratory of the University of Würzburg/FRG. Out of 467 culture proven L.m-infections in the years 1986 and 1987, two cases were localized infections of the gall bladder. Although studies of the microbiology of gallbladder infections have not demonstrated the recovery of Listeria monocytogenes as a pathogen in acute or chronic cases of cholecystitis, these two cases substantiate the existence of this rare form of listeriosis as an entity and support the theory of the importance of the gastrointestinal tract in the pathogenesis of Listeria infections.     

Listeria monocytogenes meningitis

Presented is the case of a 42-year-old, previously healthy man with meningitis secondary to Listeria monocytogenes. Following lumbar puncture, empiric therapy with intravenous penicillin was started and he was hospitalized. Subsequently L monocytogenes was proven to be the causative organism and antibiotic therapy was changed to ampicillin and gentamicin. The patient's condition improved. He was discharged and continues to do well. There was no evidence at any time of underlying predisposition to infection. Listeria meningitis in healthy patients and in patients with underlying disease is discussed, along with possible implications about choice of empiric antibiotics.     

Listeria monocytogenes in Northern Nigeria

During a one-year prospective study, the clinical conditions in which Listeria monocytogenes was isolated from nineteen patients (six females, thirteen males) included meningitis, meningoencephalitis, spontaneous peritonitis, septicaemia, arthritis, pelvic infection and urethritis. All isolates were type 4 serotype. Both apparently well persons and patients with already compromised immune systems were observed. Subtyping of ascitic fluid lymphocytes in one patient with peritonitis showed predominantly T cell subpopulation and no B cells. Most isolates were sensitive to ampicillin, crystalline penicillin and erythromycin. A mortality of 27 per cent was recorded.     

Acute Listeria monocytogenes osteomyelitis

Summary The presentation and management of a case of acute Listeria monocytogenes osteomyelitis in a lady with acute lymphoblastic leukaemia are described. The literature on Listeria infection is briefly reviewed, and the importance of scrutinizing diphtheroid-like isolates in the laboratory is stressed.

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Akute Listeria-monocytogenes-Osteomyelitis

Zusammenfassung Es werden Verlauf und Behandlung eines Falles von akuter Listeria-monocytogenes-Osteomyelitis bei einer Patientin mit akuter lymphoblastischer Leukämie beschrieben. Zusätzlich wird eine kurze Literaturübersicht von Listeria-Infektionen erstellt und die Bedeutung der exakten Diagnostik von Diphtheroiden wird hervorgehoben.

     

Listeria monocytogenes in renal transplant recipients

Five cases of Listeria monocytogenes bacteremia were observed from April to December 1985, among renal transplant recipients from the same hospital in S?o Paulo, Brazil. The patients were adults (mean age: 40.6 years), and the basic complaint was fever, with no report of meningeal syndrome. Laboratory tests revealed the presence of two serovars, (1/2)a and 4b, which were classified into three lysotypes. The four strains of serovar 4b showed the same antibiotype, with resistance to cefoxitin, clindamycin, oxacillin and penicillin.     

武汉市食品中单核细胞增生性李斯特氏菌污染状况调查

单核细胞增生性李斯特氏菌 (LM) ,是重要的食源性疾病的病原菌 ,在自然界分布广泛。该菌致病性强 ,可引起人类菌血症 ,脑膜炎等症状 ,不易被寒冷日晒等强烈因素所杀灭。国外对该菌高度重视 ,WHO将其列为九十年代食品中四大致病菌之一〔1〕。我国虽未报道单核细胞增生性李斯特氏菌食物中毒 ,但此次食品污染状况调查初步了解了单核细胞增生性李斯特氏菌在武汉市食品污染状况 ,为制定食源性致病菌的预防措施提供了依据。1　材料和方法1 1　样品来源和品种　采自武汉三镇市售生肉、熟肉、乳制品 (酸奶、果奶 )、冷饮 (冰淇淋 )、乳 (生奶、消…     

单增李斯特菌P60单克隆抗体的制备及初步应用

目的 制备并筛选单增李斯特菌P60特异性单克隆抗体,初步建立其快速检测方法。方法 以体外表达并纯化的李斯特菌P60蛋白为抗原免疫Balb/c 小鼠, 采用杂交瘤技术制备抗李斯特菌P60的McAb,鉴定其抗体亚型,并对单抗的腹水效价、稳定性及亲和力进行分析。用纯化的单克隆抗体包被酶标板,结合生物素化处理的P60多克隆抗体,夹心ELISA筛选单增李斯特菌特异性单抗,并以重组P60为标准品,建立标准曲线。结果 获得了4株可稳定分泌P60单克隆抗体的杂交瘤细胞株。ELISA检测表明,筛选的6C2单克隆抗体株可特异性与单增李斯特菌(4b)P60反应。结论 建立的夹心ELISA方法可对单增李斯特菌进行特异性鉴别和定量检测。