三氧化二砷诱导K562细胞凋亡的初步研究

目的：探讨三氧化二砷治疗慢性粒细胞性白血病（ＣＭＬ）的机制。方法：以ＣＭＬ细胞系Ｋ５６２为模型，通过细胞增殖、活力检测，形态学观察，肿瘤集落形成的琼脂糖凝胶电泳等检验。结果：经≥２．５μｍｏｌ／ＬＡｓ２Ｏ３处理的Ｋ５６２细胞可出现增殖受抑和凋亡的形态学改变及ＤＮＡ片段化。结论：Ａｓ２Ｏ３能有效地诱导Ｋ５６２细胞凋亡。     

氯化汞对K562和HEL细胞株作用的实验研究

为探讨中药水银（Ｈｇ）和经粉（ＨｇＣｌ）的抗肿瘤作用,以三氧化二砷（Ａｓ２Ｏ３）及阿糖胞苷（Ａｒａ－ｃ）为对照,应用细胞形态学、流式细胞术、ＭＴＴ法和药物反应曲线等方法观察３种药物对Ｋ５６２和ＨＥＬ细胞株的作用。结果表明,ＨｇＣｌ２和Ａｓ２Ｏ３均能诱导Ｋ５６２细胞凋亡,而Ａｒａ－ｃ无此作用。ＨｇＣｌ２可使Ｋ５６２的Ｇ０／Ｇ１增高,揭示激活Ｐ５３基因可能是氯化汞诱导凋亡机制之一;ＨｇＣｌ２和Ａｓ２Ｏ     

硫化砷诱导K562细胞凋亡

研究硫化砷（Ａｓ２Ｓ２）对Ｋ５６２细胞凋亡的诱导作用。采用Ｋ５６２细胞体外培养，应用流式细胞仪（ＦＣＭ）分析细胞周期，鉴定凋亡细胞，检测线粒体膜蛋白Ａｐｏ２．７的表达。结果显示：Ａｓ２Ｓ２能够诱导Ｋ５６２细胞凋亡，使细胞周期中的Ｓ期细胞减少，Ｇ２／Ｍ期细胞升高，提示：Ａｓ２Ｓ２具有促凋亡效应。     

反义BCR/ABL寡核苷酸体外抑制K562细胞的研究

目的：探讨反义ＢＣＲ和ＡＢＬ融合基因（ＢＣＲ／ＡＢＬ融合基因）寡核苷酸体外抑制Ｋ５６２细胞的作用及机制。方法：采用Ｋ５６２细胞培养,观察反义ＢＣＲ／ＡＢＬ寡核苷酸体外对Ｋ５６２细胞数、台盼蓝拒染率及克隆形成等的影响。结果：经反义ＢＣＲ／ＡＢＬ寡核苷酸ｂ３ａ２（ＡＳｂ３ａ２）处理后培养８ｄ的Ｋ５６２克隆抑制率达６０６９％,液体培养中细胞数从２４ｈ开始较对照组减少,细胞存活率从８ｈ开始即较对照组明显下降,ＢＣＲ／ＡＢＬ寡核苷酸ｂ２ａ２（ＡＳｂ２ａ２）及无义寡核苷酸对Ｋ５６２细胞数和存活率无明显影响;３种寡核苷酸对ＨＬ６０细胞数和存活率也无影响。结论：ＡＳｂ３ａ２对Ｋ５６２细胞有序列特异性的抑制作用。ＡＳｂ３ａ２的这种抑制作用可能在于它诱导Ｋ５６２细胞的凋亡。     

复方苦参增加 ＮＫ 细胞对白血病干细胞杀伤作用的实验研究

目的：研究复方苦参对自然杀伤细胞（ＮＫ 细胞）杀伤白血病干细胞（ＬＳＣ）的影响及机制。方法：检 测 ＮＫ 细胞对 Ｋ ５６２ 细胞、复方苦参干预前后 ＬＳＣ 的杀伤作用,流式细胞术检测细胞凋亡情况。检测 Ｋ ５６２ 细 胞、复方苦参干预前后 ＬＳＣ 细胞表面的 ＮＫＧ２Ｄ 配体人巨细胞病毒 ＵＬ １６ 蛋白的结合蛋白（ＵＬＢＰ１）、ＭＨＣ－Ⅰ类 链相关分析 Ａ 或 Ｂ（ＭＩＣＡ／ Ｂ）水平。结果：复方苦参提高 ＬＳＣ 对 ＮＫ 细胞杀伤作用的敏感性。复方苦参增加 ＮＫ 细胞对 ＬＳＣ 诱导凋亡作用。复方苦参可上调 ＬＳＣ 表面的 ＮＫＧ２Ｄ 配体 ＵＬＢＰ１、ＭＩＣＡ／ Ｂ 水平。结论：复方苦参增 强 ＬＳＣ 对 ＮＫ 细胞杀伤的敏感性和凋亡诱导作用。     

影响白血病细胞株 K_(562) 对 LDL-ACM 复合物摄取因素的研究

以低密度脂蛋白（ＬＤＬ）为阿克拉霉素（ＡＣＭ）的载体，以游离ＡＣＭ为对照，体外研究ＬＤＬ－ＡＣＭ复合物对白血病细胞株Ｋ５６２的细胞毒作用及其影响因素。结果显示：（１）Ｋ５６２细胞对ＬＤＬ－ＡＣＭ复合物摄取较游离ＡＣＭ明显增加，在前３０ｍｉｎ摄取较快，随时间延长摄取逐渐减慢；（２）过量的天然ＬＤＬ、肝素及低温均可使细胞摄取复合物明显减少，而过量的ＨＤＬ则无影响，去脂蛋白血清预刺激的细胞摄取复合物明显增多；（３）对Ｋ５６２细胞的细胞毒作用随复合物浓度增加而逐渐增强，达１０ｍｇ／Ｌ以上时，增加药物浓度其细胞毒作用不再加强。     

反义bcr／abl寡核苷酸诱导K562细胞凋亡时Caspase3表达及？…

用反义ｂｃｒ／ａｂｌ寡核苷酸片段诱导Ｋ５６２细胞凋亡,观察Ｃａｓｐａｓｅ３表达及活性变化,以阐明Ｃａｓｐａｓｅ在慢性髓性白血病（ＣＭＬ）发病机制中的作用。方法：用反义ｂｃｒ／ａｂｌ寡核苷酸片段ＡＳ和对照无义片段ＮＳ自理５６２,ＳＷＩＭＸＬＥＱＵＭＦ ＹＮＶ ＥＹ display status     

ODC反义寡核苷酸对K562细胞凋亡及分化和相关基因表达的影响

目的：观察鸟氨酸脱羧酶反义寡核苷酸（ＯＤＣａｓＯＤＮ）引起鸟氨酸脱羧酶（ＯＤＣ）表达及活性的抑制对人红白血病Ｋ５６２细胞生长、凋亡的影响,为探索抑制多胺生物合成的抗癌新途径提供实验依据。方法：采用硫代修饰的ＯＤＣａｓＯＤＮ处理细胞,观察生长曲线,及用Ｇｉｅｍｓａ染色观察形态变化,ＲＮＡ斑点杂交检测基因表达,放射微量法测定ＯＤＣ活性。结果：ＯＤＣａｓＯＤＮ能抑制Ｋ５６２细胞生长、诱导凋亡,并证明了该细胞的ＯＤＣ、Ｂｃｌ２表达下降及ＯＤＣ活性受到抑制。结论：ＯＤＣａｓＯＤＮ能下调ＯＤＣ表达、降低其酶活性,引起ｂｃｌ２基因表达抑制,导致Ｋ５６２细胞生长抑制及凋亡诱导。     

螺旋藻多糖对CD3AK细胞增殖能力的影响

研究了螺旋藻多糖（ＰＳ）对ＣＤ３ＭｃＡｂ激活的杀伤细胞（ＣＤ３ＭｃＡｂ ａｃｔｉｖａｔｅｄ ｋｉｌｌｅｒ ｃｅｌｌｓ，ＣＤ３ＡＫ ｃｅｌｌｓ）增殖能力的影响。结果表明，当ＰＳ浓度为２．５μｇ／ｍｌ培养体系条件下，对ＣＤ３ＡＫ细胞具有明显的刺激细胞增殖作用（Ｐ〈０．０２）；对培养长达２３ｄ的ＣＤ３ＡＫ细胞杀伤肿瘤细胞（ＫＬ５６２细胞）的活性仍维持在较高的水平（４６．５％￣５０％）。     

三氧化二砷诱导血管平滑肌细胞凋亡的实验研究

目的：探讨三氧化二砷（Ａｓ２Ｏ３）诱导大鼠血管平滑肌细胞（ＶＳＭＣｓ）调亡的作用。方法：建立大鼠ＶＳＭＣｓ增生模型。用四甲基偶氮唑蓝还原反应（ＭＴＴ法）、透射电镜、琼脂糖凝胶电泳、流式细胞术等方法观察Ａｓ２Ｏ３诱导ＶＳＭＣｓ凋亡的作用。结果：经Ａｓ２Ｏ３作用后ＶＳＭＣｓ出现典型的凋亡变化,透射电镜下可风细胞核仁消失,染色质浓缩聚集于核膜下。ＤＡ琼脂糖凝胶电泳出现凋亡特征性梯形电泳条带。汉式细胞术检     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.