Th1/Th2细胞因子与蠕虫感染

蠕虫感染后引起复杂的免疫应答中辅助性T细胞处于核心位置,Th1/Th2细胞分泌的细胞因子在蠕虫感染后表达地位的不同,将决定蠕虫感染的发展与转归。本文对Th1/Th2细胞因子在蠕虫感染中的表达研究进展予以综述。     

Th1/Th2及Tc1/Tc2在胃癌患者外周血中的漂移及意义

目的: 研究胃癌患者外周血Th1/Th2及Tc1/Tc2漂移状况,并分析其免疫预存状态.方法: 2008-10-21/2008-12-05中国人民解放军总医院普通外科新入院的术前胃癌患者外周血样本25例,同期健康献血员正常对照组外周血样本25例.四色流式细胞仪检测胃癌患者及正常对照人群外周血激活的淋巴细胞内细胞因子(IFN-γ、IL-4),并分析Th1/Th2及Tc1/Tc2漂移状态.结果: 胃癌患者外周血Th1/PBL、Tc1/PBL、Th1/Th2、Tc1/Tc2比值均较正常对照组明显减少(6.242%±4.078% vs 3.047%±1.710%,14.171%±8.984% vs 6.393%±5.235%,3.127%±3.633% vs 1.172%±0.300%,17.200%±25.930% vs 3.252%±8.732%,均P＜0.01).结论: 胃癌患者Th1及Tc1细胞比例减少,并出现Th1/Th2及Tc1/Tc2漂移现象,提示胃癌患者预存免疫状态低下,机体抗肿瘤免疫功能抑制.     

胰岛素对体外培养人淋巴细胞生成Th1/Th2因子平衡的调节作用

目的 探讨胰岛素（Ins）及Ins受体对免疫系统功能的调节作用。了解Ins对体外培养人Th细胞分泌Th1／Th2类细胞因子的影响。方法 用4种Ins浓度的无血清培养基体外培养、激活人外周血淋巴细胞，ELISA法测定其IL-4、IFN了水平。结果A、B组（Ins浓度分别为10、1nmoi／L）的INF-γ水平低于C、D组（Ins浓度分别为0．1、0nmol／L）（P＜0．05）。而IL-4水平4组间比较差异无统计学意义。A、B组的INF-γ/IL-4比值均显著低于C、D组（P〈0．05）。结论 Ins调节体外培养人淋巴细胞的Th1／Th2平衡。     

慢性乙型重型肝炎患者外周血Th1/Th2,Tc1/Tc2的表达

分析慢性乙型重型肝炎患者外周血Th1/Th2,Tc1/Tc2的表达特点,探讨其与重型肝炎的关系.用流式细胞仪检测31例重型肝炎和20例正常对照者外周血T细胞胞内细胞因子IFN-γ、IL-4的百分率,发现1.重型肝炎Th1、Th2、Tc1分别为(27.41±4.88)%、(3.48±2.67)%、(43.85±12.98)%,显著高于正常对照,且以Th1和Tc1表达为主;2.重症期与恢复期相比,康复后Th1、Th2、Tc1显著下降,尤以Tc1下降更明显(P均＜0.01);3.生存组与死亡组比较,Th1、Tc1无显著性差异(P＞0.05).Th1、Th2、Tc1与重型肝炎的发生转归有密切关系,动态检测可作为监测病情和判断预后的指标.     

慢性乙型重型肝炎患者外周血Th1/Th2,Tcl/Tc2的表达

分析慢性乙型重型肝炎患者外周血Th1/Th2 ,Tc1/Tc2的表达特点,探讨其与重型肝炎的关系。用流式细胞仪检测31例重型肝炎和2 0例正常对照者外周血T细胞胞内细胞因子IFN -γ、IL - 4的百分率,发现:1 重型肝炎Th1、Th2、Tcl分别为(2 7 .4 1±4 . 88) %、(3 .4 8±2 . 6 7) %、(43 .85±12 . 98) % ,显著高于正常对照,且以Th1和Tcl表达为主;2 重症期与恢复期相比,康复后Th1、Th2、Tcl显著下降,尤以Tcl下降更明显(P均<0. 0 1) ;3 生存组与死亡组比较,Th1、Tcl无显著性差异(P >0 . 0 5 )。Th1、Th2、Tcl与重型肝炎的发生转归有密切关系,动态检测可作为监测病情和判断预后的指标。     

Recovery from Strongyloides venezuelensis infection in Lewis rats is associated with a strong Th2 response

In this study, we investigated the characteristics of the infection and subsequent immunity induced by Strongyloides venezuelensis in Lewis rats. Animals were infected with 4000 L3 of S. venezuelensis and number of eggs per gram of faeces indicated an acute phase around day 8 and a recovery phase around day 32 after infection. A strong Th2 polarization during recovery phase was ascertained by a significant increase in IgG1 and IgE compared with that in the acute period. A shift in the cytokine profile confirmed these findings. A predominant production of IFN-γ during the acute phase was followed by IL-10 production during recovery. Together these findings show that experimental infection of Lewis rats with S. venezuelensis presents a kinetics of parasite establishment and immunity similar to that described in other models of helminthic infection.     

Activated rat hepatic stellate cells influence Th1/Th2 profile in vitro

AIM: To investigate the effects of activated rat hepatic stellate cells(HSCs) on rat Th1/Th2 profile in vitro.METHODS: Growth and survival of activated HSCs and CD4+ T lymphocytes cultured alone or together was assessed after 24 or 48 h. CD4+ T lymphocytes were then cultured with or without activated HSCs for 24 or 48 h and the proportion of Th1 [interferon(IFN)-γ+] and Th2 [interleukin(IL)-4+] cells was assessed by flow cytometry. Th1 and Th2 cell apoptosis was assessed after 24 h of co-culture using a caspase-3 staining procedure. Differentiation rates of Th1 and Th2 cells from CD4+ T lymphocytes that were positive for CD25 but did not express IFN-γ or IL-4 were also assessed after 48 h of co-culture with activated HSCs. Galectin-9 expression in HSCs was determined by immunofluorescence and Western blotting. ELISA was performed to assess galectin-9 secretion from activated HSCs.RESULTS: Co-culture of CD4+ T lymphocytes with activated rat HSCs for 48 h significantly reduced the proportion of Th1 cells compared to culture-alone conditions(-1.73% ± 0.71%; P 0.05), whereas the proportion of Th2 cells was not altered; the Th1/Th2 ratio was significantly decreased(-0.44 ± 0.13; P 0.05). In addition, the level of IFN-γ in Th1 cells wasdecreased(-65.71 ± 9.67; P 0.01), whereas the level of IL-4 in Th2 cells was increased(82.79 ± 25.12; P 0.05) by co-culturing, as measured by mean fluorescence intensity by flow cytometry. Apoptosis rates in Th1(12.27% ± 0.99%; P 0.01) and Th2(1.71% ± 0.185%; P 0.01) cells were increased 24 h after co-culturing with activated HSCs; the Th1 cell apoptosis rate was significantly higher than in Th2 cells(P 0.01). Galectin-9 protein expression was significantly decreased in HSCs only 24 h after coculturing(P 0.05) but not after 48 h. Co-culture for 48 h significantly increased the differentiation of Th1 and Th2 cells; however, the increase in the proportion of Th2 cells was significantly higher than that of Th1 cells(1.85% ± 0.48%; P 0.05).CONCLUSION: Activated rat HSCs lower the Th1/Th2 profile, inhibiting the Th1 response and enhancing the Th2 response, and this may be a novel pathway for liver fibrogenesis.     

结缔组织病相关性间质性肺疾病患者外周血Th1/Th2细胞的表达

目的探讨外周血Th1/Th2及细胞因子在结缔组织病相关性间质性肺疾病的表达及意义。方法收集23例正常对照组及28例病理类型为非特异性间质性肺炎(NSIP)的结缔组织病相关性间质性肺疾病(CTD-ILD)患者的外周血单个核细胞及血浆,分别检测PBMC中Th1/Th2的比例及血浆中Th1/Th2及细胞因子的表达情况。结果与正常对照组相比,CTD-ILD患者组Th1比例明显增加,差别具有统计学意义(P=0.04)。而Th2比例明显降低(P=0.000),伴随着Th1/Th2比例的升高(P=0.001)。CTD-ILD患者TNF-α,IFN-γ细胞因子的浓度较正常对照组的血浆浓度均明显增加(P0.05)。IL-4的浓度较正常对照组的血浆浓度降低(P0.05)。结论在CTD-ILD患者体内的存在Th1/Th2平衡失调,向Th1方向偏移。     

猪带绦虫抗原刺激囊虫病患者PBMC Th1/Th2细胞因子的表达及其免疫调节

目的检测猪带绦虫不同虫期抗原刺激囊虫病患者外周血单个核细胞(PBMC)Th1/Th2细胞因子的表达水平,并分析其在囊虫病免疫调控中的作用。方法用流式细胞仪检测囊虫病患者新鲜血T淋巴细胞亚群,并以猪带绦虫六钩蚴抗原或囊尾蚴抗原刺激囊虫病患者PBMC,分别在刺激的第0d、5d、10d和15d时检测Th1/Th2细胞因子(IFN-γ及IL-4),对分泌IFN-γ或IL-4的不同细胞群体进行数据分析。结果囊虫病患者新鲜血T淋巴细胞亚群CD3+与CD4+细胞较正常人升高,CD4+/CD8+较正常人升高,分泌IFN-γ及IL-4的CD3+细胞百分率较正常人升高;猪囊尾蚴抗原刺激囊虫病患者PB-MC第0d、5d、10d和15d时,分泌IFN-γ的CD3+细胞百分率分别为25.42%±5.53%,30.46%±4.94%,36.52%±4.73%,38.69%±5.58%;分泌IL-4的CD3+细胞百分率分别为2.52%±0.52%,3.00%±0.57%,3.81%±0.70%,5.03%±0.73%。六钩蚴抗原刺激囊虫病患者PBMC分泌IFN-γ及IL-4的CD3+细胞百分率亦呈现相同的变化趋势。结论囊虫病患者与正常人相比PBMC中存在T淋巴细胞的极化水平异常,CD3+与CD4+细胞百分率均显著升高,T细胞亚群比例失调,免疫功能紊乱;随着囊尾蚴抗原刺激外周血PBMC时间延长,分泌IFN-γ和IL-4的CD3+细胞百分率逐渐升高。     

大鼠支气管哮喘模型γδT细胞Th1/Th2免疫应答模式的研究

目的　探讨γδT细胞在哮喘的免疫应答模式 ,认识γδT细胞亚群在哮喘发病机制中的作用。方法　Wistar大鼠 2 0只 ,随机分为健康对照组与哮喘组 (用鸡卵清蛋白致敏和刺激大鼠 ,制作哮喘模型 ) ,每组 10只。收集外周血单个核细胞 (PBMC)和支气管肺泡灌洗液 (BALF) ,用补体攻击法结合洗淘法选择性培养扩增γδT细胞 ,并用流式细胞术鉴定培养体系中的γδT细胞及其纯度 ,用原位杂交法测γδT细胞白细胞介素 (IL) 4mRNA和干扰素 (IFN)γmRNA的表达 ,用ELISA检测培养上清液中IL 4和IFN γ的浓度。结果　哮喘组大鼠PBMC和BALF中 ,γδT细胞培养上清液中IL 4浓度显著高于健康对照组 (P <0 0 1) ,IFN γ浓度低于健康对照组 (P <0 0 1) ;γδT细胞IL 4mRNA表达阳性率高于健康对照组 (P <0 0 1) ,IFN γmRNA表达阳性率低于健康对照组 (P <0 0 1)。结论γδT细胞或者γδT细胞亚群存在Th1/Th2模式 ,在大鼠哮喘模型呈Th2优势应答 ,γδT细胞参与了哮喘的发病过程。     

α黑素细胞刺激素对CIA大鼠CD28和CD86分子及Th1/Th2型细胞因子的影响

目的观察α黑素细胞刺激素(α-MSH)对胶原性关节炎(CIA)大鼠免疫细胞表达CD28和CD86分子及其产生Th1／Th2型细胞因子的影响，探讨α-MSH抑制CIA发病的相关免疫学机制、方法建立大鼠CIA动物模型，分离培养致敏大鼠脾细胞或腹股沟淋巴结细胞，加入α-MSH(10^-12～10^-6mol／L)和(或)100μg／ml牛Ⅱ型胶原(bCⅡ)，^5H脱氧胸苷(TdR)掺入法测定α-MSH对淋巴细胞增生反应的影响；收集上清，用酶联免疫吸附试验(ELISA)测定白细胞介素(IL)-1β、IL-10及干扰素(IFN)-γ含量，以L929为靶细胞．采用细胞毒活性四甲基偶氮唑蓝(MTT)显色法测定肿瘤坏死因子(TNF)-α浓度，硝酸还原酶法测定一氧化氮(NO)分泌水平：应用荧光激活细胞分类(FACS)检测α-MSH对T淋巴细胞表达CD28分子和巨噬细胞表达CD86分子的影响、结果α-MSH能显著抑制bCⅡ诱导的CIA大鼠致敏淋巴细胞的增生反应，并显著抑制CIA大鼠致敏淋巴细胞表达CD28分子和腹腔巨噬细胞表达CD86分子：显著减少IFN-1、IL-1和TNF-α的产生，增加IL-10的分泌。结论α-MSH有抑制CIA大鼠发病及抑制软骨破坏的作用，其机制与抑制CD28和CD86分子表达、调节细胞因子及NO的水平有关。     

刚地弓形虫感染致Th1/Th2细胞因子变化对大鼠生精细胞的影响

目的 研究弓形虫感染后雄性大鼠Th1、Th2细胞因子IFN-γ、IL-4及一氧化氮(NO)水平的变化,探讨其对雄性睾丸生精细胞损伤的影响.方法 选用9～10周龄雄性SD大鼠132只,按随机数字表法分为3组:正常对照组、低剂量感染组(1×102个速殖子)、高剂量感染组(1×104个速殖子),感染后第0、3、6、9、……30天,感染组和对照组分别取4只大鼠,检测大鼠血清IFN-γ、IL-4及NO的水平,并且做睾丸的病理学检查和精子计数.结果 弓形虫感染组大鼠血清IFN-γ显著升高,低剂量组及高剂量组在第6天达峰值,分别为(286.3±45.3) pg/ml和(506.6±34.3) pg/ml;血清NO亦显著升高,高剂量组第9天达峰值(77.7±7.0) μmol/L,低剂量组第12天达峰值(59.5±5.3) μmol/L,之后均迅速降低;感染组大鼠血清IL-4逐渐升高,低剂量组及高剂量组于第15天达峰值(233.3±36.9) pg/ml、(366.7±52.4) pg/ml后逐渐降低.以上指标至实验末期均未恢复正常水平.病理检查显示,正常对照组大鼠生精小管层次清晰,细胞丰富,精子计数为175.7±23.7,感染组大鼠生精小管层次混乱,精母细胞、精子细胞明显减少,低剂量组及高剂量组至第15天达谷值84.5.23.5、47.3±14.7,至实验末期无明显恢复.结论 弓形虫感染导致的早期Th1细胞因子IFN-γ极化及NO显著升高,随后引起Th2细胞因子IL-4反应性增高,并介导以精母细胞为主的生精细胞损伤及生精障碍.     

Trichinella spiralis antigens prime mixed Th1/Th2 response but do not induce de novo generation of Foxp3+ T cells in vitro

Many parasitic helminth infections induce Th2-type immune responses and engage the regulatory network. In this study, we specifically investigated the influence of antigens derived from different life stages of the helminth Trichinella spiralis on the polarization of naive CD4(+) T cells by dendritic cells. Results obtained from C57BL/6 mice showed that T. spiralis derived antigens have the capacity to induce bone marrow-derived dendritic cells to acquire an incompletely mature phenotype that promotes a significant proliferation of naive CD4(+) T cells and a mixed Th1/Th2 cytokine profile with the predominance of Th2 cytokines. Increased production of IL-4, IL-9, IL-10 and IL-13 accompanied increased IFN-γ. Furthermore, dendritic cells pulsed with T. spiralis antigens did not induce an increase in the population of Foxp3(+) T regulatory cells. Although other helminth antigens have demonstrated the capacity to induce de novo generation of Foxp3(+) T regulatory cells, here our in vitro studies provide no evidence that T. spiralis antigens have this capacity.     

初治肺结核患者外周血Th1/Th2细胞的测定及临床意义

目的　探讨外周血CD 4 T淋巴细胞及其亚型 (Th1/Th2 )细胞在初治肺结核患者中的改变及临床意义 ,并了解其在结核病治疗过程中的变化。方法　利用流式细胞术对 10 5例初治肺结核患者、2 5名正常人外周血CD 4 T淋巴细胞进行直接计数 ,同时用 2 5ng/ml佛波酯 (PMA)、2 5 0ng/ml离子霉素 (ionomycin)对外周血细胞进行刺激 ,用 2 μmol/ml莫能霉素 (monensin)作蛋白转运抑制剂 ,5 %CO2培养 4 0～ 4 5h ,而后分别配对加入CD3 PC5 CD8 FITC INF γ PE/CD3 PC5 CD8 FITC IgG1 PE、CD3 PC5 CD8 FITC IL 4 PE/CD3 PC5 CD8 FITC IgG1 PE(PC5 :藻红蛋白 花青苷 5 ;FITC :异硫氰酸胍荧光素 ;PE :藻红蛋白 )单克隆抗体进行胞膜和胞内标记 ,进行流式细胞术检测Th1/Th2的百分含量。按病变程度分为重度 32例 ,中度 4 4例 ,轻度 2 9例。并对其中 6 7例肺结核患者在化疗强化期末、第 6个月化疗结束时进行同样指标的追踪检测 ,以了解在抗结核治疗过程中肺结核患者外周血CD 4 T细胞、Th1/Th2细胞的变化状况。结果　 (1)初治肺结核患者外周血CD 4 T细胞、Th1细胞水平显著低于健康对照组 ,其检测值分别为 (6 6 3± 16 0 )个 / μl、(9 5 6±3 6 0 ) %和 (735± 15 6 )个 / μl、(18 70± 5 0 3) % (P值均 <0 0 5     

从Th1/Th2、Treg/Th17之间的平衡研究轻、中度慢性乙型肝炎患者CD4~+T淋巴细胞的免疫失衡状态

目的从Th17及其相关细胞因子与Th1、Th2、Treg的相互关系,探讨轻、中度CHB患者CD4~+T淋巴细胞的整体免疫失衡状态。方法选择轻、中度CHB患者,与健康志愿者进行比较,观察其外周血各免疫细胞(Th17、Th1、Th2、Treg、Th1/Th2、Treg/Th17)、相关细胞因子(IL-17、IL-6、TGF-β、IFN-γ、IL-4)、主要效应分子(穿孔素、颗粒酶B)和核转录因子(孤独核受体RORγt、Foxp3等)的变化及其相互关系。结果与健康志愿者相比,CHB患者CD8~+T淋巴细胞比例更高,CD4~+/CD8~+比例显著下降,差异有统计学意义(P0.01);与健康志愿者相比,CHB患者Th1细胞比例更高,Th1/Th2显著升高,差异有统计学意义(P0.01);与健康志愿者相比,CHB患者Th17细胞比例更高,Treg/Th17比例显著下降,差异有统计学意义(P0.01);与健康志愿者相比,CHB患者穿孔素和颗粒酶B的表达更高,差异有统计学意义(P0.01);与健康志愿者相比,CHB患者FOXP3、IL17mRNA和RORγt的表达差异无统计学意义(P0.05);与健康志愿者相比,CHB患者TGF-B1、IL-6、IL-17显著增高,差异有统计学意义(P0.01);IFN-γ和IL-4的表达差异无统计学意义(P0.05)。结论轻、中度CHB患者在免疫细胞及其相关细胞因子、效应分子等各环节,尤其是Treg/Th17比例,均处于严重的免疫失衡状态。     

T‐cell clones in human trichinellosis: Evidence for a mixed Th1/Th2 response

In humans, studies on the cellular immune response against Trichinella are scarce. Aim of this study was to characterize the cytokine profile of T cells specific for Trichinella britovi in trichinellosis patients. Peripheral blood mononuclear cells (PBMC) were obtained from five patients involved in a trichinellosis outbreak caused by T. britovi, which occurred in 2013 in Tuscany (Italy). All the patients resulted positive for Trichinella‐specific IgG, IgE and presented eosinophilia. T cells were investigated for their proliferation to excretory/secretory antigens from Trichinella spiralis muscle larvae (TsES) and for their cytokine profile. A total of 284 CD4+ and 42 CD8+ T‐cell clones were obtained from the TsES‐specific T‐cell lines from PBMC. All T‐cell clones proliferated in response to mitogen. Of the 284 CD4+ T‐cell clones generated from TsES‐specific T‐cell lines, 135 (47%) proliferated significantly to TsES; 26% CD8+ T‐cell clones showed proliferation to TsES. In the series of the 135 TsES‐specific CD4+ clones, 51% expressed a Th2 profile, 30% a Th0 and 19% Th1. In the series of the 11 TsES‐specific CD8+ T‐cell clones, 18% were Tc2, 45% Tc0 and 36% Tc1. In human trichinellosis, the cellular immune response is, during the chronic phase, mixed Th1/Th2.     

Filaria/Wolbachia activation of dendritic cells and development of Th1-associated responses is dependent on Toll-like receptor 2 in a mouse model of ocular onchocerciasis (river blindness)

Toll-like receptors (TLRs) regulate dendritic cell function and activate signals that mediate the nature of the adaptive immune response. The current study examined the role of TLRs in dendritic cell activation and in regulating T cell and antibody responses to antigens from the filarial parasites Onchocerca volvulus and Brugia malayi, which cause river blindness and lymphatic filariasis, respectively. Bone-marrow-derived CD11c(+) cells from C57BL/6 and TLR4(-/-) mice produced high levels of IL-6 and RANTES, and showed elevated surface CD40 expression, whereas CD11c(+) cells from myeloid differentiation factor 88(-/-) (MyD88(-/-)), TLR2(-/-) and TLR2/4(-/-) mice were not activated. Similarly, IFN-gamma production by splenocytes from immunized TLR2(-/-) mice was significantly impaired compared with splenocytes from C57BL/6 and TLR4(-/-) mice. In contrast, there was no difference among these strains in Th2-associated responses including IL-5 production by splenocytes from immunized animals, serum IgE and IgG(1), or eosinophil infiltration into the corneal stroma. Neutrophil recruitment to the cornea and CXC chemokine production was inhibited in immunized TLR2(-/-) mice compared with C57BL/6 and TLR4(-/-) mice. Taken together, these findings demonstrate an essential role for TLR2 in filaria-induced dendritic cell activation, IFN-gamma production and neutrophil migration to the cornea, but does not affect filaria-induced Th2-associated responses.     

初发系统性红斑狼疮患者Th1/Th2及其调控因子IL-10 IL-12基因研究

目的：探讨初发系统性红斑狼疮（SLE）病人Th1/Th2分布及其调控细胞因子、细胞因子受体基因表达的差异。方法：运用三色荧光标记法流工细胞术检测42例未红药物治疗初发狼疮病人，T细胞亚群分布，并以10名正常人作对照；同时运用ABI－7700实时监测定量PCR法检测其中38例病人和28名正常人IL－10／IL－12 mRNA及其受体表达的差异。结果：①初发SLE病人Th1较正常人明显减低（P＜0．05）；但Th1/Th2无显著性改变。②与正常组相比，SLE组病人IL－10mRNA表达差异无显著性，但IL－10R表达明显升高（P＜0．05）；SLE组病人IL－12mRNA及其受体表达较正常人明显降低（P均＜0．05）。③红斑组病人Th1/Th2、IL－12p35较正常组降低（P均＜0．05）；IL－10R较正常组显著增高IP＜0．05）。④RNP阳性组病人IL－10、IL－12p40较正常组升高，IL－2p35较正常组降低（P均＜0．05）。结论：SLE是一种以Th1细胞下降，Th2细胞相对占优势的免疫介导的自身免疫性疾病，源于诱导向Th1细胞分化的IL－12及其受体养活和细胞因子间失衡所致。     

MicroRNA 在调控支气管哮喘 Th1/Th2免疫应答中的研究进展

MicroRNA 是一类单链非编码的小分子 RNA,它通过降解目标 mRNA 或者抑制其翻译对基因进行转录后调控。Th1/Th2免疫应答失衡在支气管哮喘发病机制中发挥关键作用。新近研究表明,miRNA 在调控 Th1/Th2平衡及其相关细胞因子表达方面发挥重要作用,与支气管哮喘的发病机制密切相关,就此作一综述。     

尿毒症血液透析患者血清瘦素和Th1/Th2型细胞因子平衡的临床研究

目的 观察尿毒症血液透析(HD)患者血清瘦素、Th1/Th2型细胞因子水平的改变及其相互关系,探讨其在HD患者免疫功能异常中的作用.方法 选择终末期尿毒症行HD患者35例及健康对照者20例,双抗体夹心酶联免疫吸附法(ABC-ELISA)测定血清白细胞介素(IL)-2、干扰素(IFN)-γ、IL-4和IL-10水平,放射免疫分析法(RIA)测定两组血清瘦素水平.直线相关分析它们之间的相关性.结果 HD患者血清瘦素水平较对照组明显升高(P<0.01),与男性患者相比,女性患者升高尤为显著(P<0.01).HD患者血清Th1型细胞因子IL-2、IFN-γ水平明显升高,与对照组比较差异均有统计学意义(P<0.01);Th2型细胞因子IL4、IL-10水平较对照组降低,但差异无统计学意义(P>0.05),Th1/Th2比值升高.直线相关分析表明HD患者血清瘦素水平与IL-2(r=0.43,P<0.01)、IFN-γ(r=0.51,P<0.01)呈显著正相关,与IL-4(r=-0.39,P<0.01)、IL-10(r=-0.32,P<0.05)呈负相关.结论 HD患者存在高瘦素血症及以Th1型细胞因子占优势的Th1/Th2细胞因子平衡失调;患者血清细胞因子水平改变与瘦素水平相关.