流行性出血热各期磷脂酶A2,血栓素B2与6—酮前列腺素F1α…

用微量酸滴定法和放免分析法分别对４３例流行性出血热患者各期血清中磷脂酶Ａ２（ＰＬＡ２）活性和血浆中血栓素Ｂ２（ＴＸＢ２）和６－酮前列腺素Ｆ１α－（６－ｋｅｔｏ－ＰＧＦ１α）水平进行了动态检测。结果显示：本病各期ＰＬＡ２和ＴＸＢ２明显升高，而６－ｋｅｔｏ－ＰＧＦ１α明显下降；上述变化程度与病情高度平行，本病危重型和病的极期变化最明显，并与血小板数量下降和肾功损害相关。初步探讨了ＰＬＡ与ＴＸＢ２－６－     

流行性出血热各期磷脂酶A_2、血栓素B_2与6－酮前列腺素F_（1α）的变化

用微量酸滴定法和放免分析法分别对４３例流行性出血热患者各期血清中磷脂酶Ａ＿２（ＰＬＡ＿２）活性和血浆中血栓素Ｂ＿２（ＴＸＢ＿２）和６－酮前列腺素Ｆ＿１α（６－ｋｅｔｏ－ＰＧＦ＿１α）水平进行了动态检测。结果显示：本病各期ＰＬＡ＿２和ＴＸＢ＿２明显升高，而６－ｋｅｔｏ－ＰＧＦ＿１α明显下降，上述变化程度与病情高度平行，本病危重型和病的极期变化最明显，并与血小板数量下降和肾功损害相关。初步探讨了ＰＬＡ与ＴＸＢ＿２－６－ｋｅｔｏ－ＰＧＦ＿１α平衡间的关系及各自在本病发生、发展过程的病理生理意义。     

白细胞介素8和一氧化氮在家兔内毒素性急性损伤中的作用

目的和方法：采用间隔２４ｈ两次注射大肠杆菌内毒素（ＥＴ）的方法，复制家兔内毒素性急性肺损伤模型，探讨肺损伤的机理，结果：ＥＴ组血浆，肺组织匀浆及支气肺泡灌洗液中白细胞介素８（ＩＬ－８），亚硝酸／硝酸根离子显著增高（Ｐ〈０．０１），血浆补体Ｃ５ａ活性明显增高（Ｐ〈０．０１），ＢＡＬＦ内中性粒细胞明显增多，肺系数，肺水含量及通透指数升高，ＩＬ－８，ＮＯ２＾－／ＮＯ３＾－水平以及血清和ＢＡＬＦ中酸性磷酸     

白细胞介素8和一氧化氮在家兔内毒素性急性肺损伤中的作用

目的和方法：采用间隔２４ｈ两次注射大肠杆菌内毒素（ＥＴ）的方法，复制家兔内毒素性急性肺损伤模型，探讨肺损伤的机理。结果：ＥＴ组血浆、肺组织匀浆及支气肺泡灌洗液（ＢＡＬＦ）中白细胞介素８（ＩＬ－８）、亚硝酸／硝酸根离子（ＮＯ２－／ＮＯ３－）水平显著增高（Ｐ＜００１），血浆补体Ｃ５ａ活性明显增高（Ｐ＜００１），ＢＡＬＦ内中性粒细胞明显增多，肺系数、肺水含量及通透指数升高。ＩＬ－８、ＮＯ２－／ＮＯ３－水平以及血清和ＢＡＬＦ中酸性磷酸酶活性变化高度相关。结论：ＩＬ－８、一氧化氮（ＮＯ）参与内毒素性急性肺损伤，而ＩＬ－８是造成肺损伤的重要中间环节。     

内毒素性急性肺损伤大鼠白细胞介素—6含量改变

采用白细胞介素－６（Ｉｎｔｅｒｌｅｕｋｉｎ６，ＩＬ－６）依赖细胞株７ＴＤ１及ＭＴＴ比色法，动态观察了正常及内毒素注射后不同时间大鼠血清和支气管肺泡灌洗液（ｂｒｏｎｃｈｏａｌｏａｌｖｅｏｌａｒｌａｖａｇｅｆｌｕｉｄ，ＢＡＬＦ）中ＩＬ－６含量的变化。同时，还对各组大鼠的肺体指数和ＢＡＬＦ中蛋白质含量进行了测定，结果显示：大鼠注射内毒素后１，３，６，１２ｈ其血清和ＢＡＬＦ中ＩＬ－６含量急剧升高（Ｐ〈０．     

磷脂酶A2抑制剂对大鼠油酸性肺损伤及肾上腺素能受体的影响

本文观察了磷酯酶Ａ２（ＰＬＡ２）非特异抑制剂漏对于油酸性肺损伤大鼠肺组织ＰＬＡ２活性、肾上腺素能和受体（和受体）及病理学改变等的影响。结果显示，ＣＱ防治组肺组织ＰＬＡ２活力（９５＋５Ｕ）明显低于致伤组（２２５＋２０Ｕ）：受体Ｂｍａｘ（４９４＋３８ｆｍｏｌ／ｍｇ蛋白质）显著高于致伤组（３０７＋５５ｆｍｏｌ／ｍｇ蛋白质）；受体Ｂｍａｘ（７４＋１１ｆｍｏｌ／ｍｇ蛋白质）明显低于致伤组（１２７＋１２ｆｍｏｌ／ｍｇ蛋白质）。首先发现，ＰＬＡ２抑制剂对于肺损伤大鼠肺组织肾上腺素能和受体具有调节作用。可以认为，此为其肺组织保护作用的机制之一。     

常压缺氧大鼠血浆和肺组织TXB2和6—keto—PGF1α的动态变化

本文观察常压缺氧２４ｈ及１，２，４ｗ后大鼠血小板数及聚集率和血浆及肺ＴＸＢ２和６－ｋｅｔｏ－ＰＧＦ１α的动态变化。结果表明（１）缺氧大鼠血小板数呈先增加、后恢复、再下降，血小板聚集率呈先减少、后增加再减少的动态变化。（２）血浆ＴＸＢ２和６－ｋｅｔｏ－ＰＧＦ１α均明显升高。（３）肺组织６－ｋｅｔｏ－ＰＧＦ１α升高早于ＴＸＢ２，提示ＰＧＩ２参于早期缺氧肺血管张力调节。（４）缺氧大鼠２周时肺动脉高压达到     

常压缺氧大鼠血浆和肺组织TXB_2和6－keto－PGF_（1α）的动态变化

本文观察常压缺氧２４ｈ及１，２，４ｗ（６ｈ／ｄ，６ｄ／ｗ）后大鼠血小板数及聚集率和血浆及肺ＴＸＢ＿２和６－ｋｅｔｏ－ＰＧＦ＿（１α）的动态变化。结果表明（１）缺氧大鼠血小板数呈先增加、后恢复、再下降，血小板聚集率呈先减少、后增加再减少的动态变化。（２）血浆ＴＸＢ＿２和６－ｋｅｔｏ－ＰＧＦ＿（１α）均明显升高，（３）肺组织６－ｋｅｔｏ－ＰＧＦ＿（１α）升高早于ＴＸＢ＿２，提示ＰＧＩ＿２参干旱期缺氧肺血管张力调节。（４）缺氧大鼠２周时肺动脉高压达到高峰，右心室明显肥厚，而肺组织ＴＸＢ＿２至缺氧４周后才见增加，表明ＴＸＢ＿２不是缺氧早期肺血管收缩的主要因素。     

高原（3780米）和平原绵羊内毒素急性肺损伤的比较观察

本研究比较观察了高原和平原绵羊内毒素所致的急性肺损伤。用１２只绵羊（高原组５只，平原组７只），制备肺淋巴瘘２￣３天后，静脉内注射１．５μｇ／ｋｇ大肠杆菌内毒素致伤。结果：高原组肺动脉压（Ｐｐａ）升高幅度大于平原组，持续时间也较长；肺淋巴流量（ＱＬ）高原组增加了６．２倍，平原组增加了４．４倍（Ｐ〈０．０５）；肺淋巴蛋白清除率（ＰＣ）高原组增加了８．２倍，平原组增加了４．７倍（Ｐ〈０．０５）；肺血管通     

前列腺素E1对肿瘤坏死因子水平及磷脂酶A2活性的影响

为探讨前列腺素Ｅ１（ＰＧＥ１）保护肝细胞作用机制。本实验应用Ｄ－氨基半乳糖及内毒素复制急性肝衰竭（ＡＨＦ）模型，观察ＰＧＥ１对血清肿瘤坏死因子（ＴＮＦ）水平及肝组织匀浆磷脂酶Ａ２（ＰＬＡ２）活性的影响，结果显示：ＰＧＥ１治疗组鼠血清ＴＮＦ含量及肝组织匀浆ＰＬＡ２活性明显低于对照组（Ｐ〈０．０１），肝组织坏死面积也小于对照组（Ｐ〈０．０１）。提示ＰＧＥ１保护肝细胞作用与降低ＴＮＦ合成及抑制ＰＬＡ２活     

Level of functioning of siblings and parents of probands of varying degrees of retardation

A further analysis of already published data supports the position that retardates of low ability level less frequently have retarded siblings, retarded parents, and parents low in occupational level than do retardates higher in ability level. The analysis supports the position that there are two types of retarded individuals, persons retarded as a result of gene or chromosomal anomalies, brain injury, etc., who more frequently occur in the lower-level retardate group, and persons whose retardation represents polygenic segregation, who more frequently occur in the higher-level group.     

局部注射辛伐他汀对骨质疏松大鼠股骨髁骨小梁的改建效应

背景：局部注射具有成骨作用的辛伐他汀,可显著增加骨质疏松大鼠股骨颈及股骨髁部的骨密度及力学强度,分析局部注射辛伐他汀对股骨髁骨小梁的影响。 目的：进一步研究骨质疏松大鼠股骨内局部注射辛伐他汀对股骨髁骨小梁的影响。为将辛伐他汀应用于临床骨质疏松局部治疗提供实验基础。 方法：18只雌性SD大鼠双侧卵巢切除后3个月,制备大鼠骨质疏松模型。实验大鼠随机数字表法均分为3组,分别在实验大鼠的右侧股骨髓腔内单次注射辛伐他汀溶液5 mg、10 mg,对照组单纯注射空白载体。分别在注射后1个月处死大鼠并取材。Micro-CT扫描并定量分析骨组织形态变化。 结果与结论：给药后1个月,Micro-CT扫描结果显示,辛伐他汀治疗组的骨微结构参数如骨皮质厚度、骨小梁密度及连接率明显优于对照组。说明疏松骨骼单次注射小剂量辛伐他汀可显著促进股骨髁部骨小梁改建,改善骨骼微结构,可为强化局部、防治骨质疏松骨折的新选择进一步提供实验基础。     

石斛属民族药用植物的分类及生药学研究

中药石斛早在《神农本草经》中就被列为上品,其应用历史悠久,具有养阴生津、补肾益气、润喉护嗓、活血明目、抗癌防老等功效。本文对国内外石斛属民族药用植物的分类及生药学研究进行了综述。     

膝关节骨性关节炎的关节镜治疗体会

目的我科自2001～2005年4月对59例67膝的骨性关节炎（0A），进行关节镜检查及镜下清理术。方法镜检：膝关节骨性关节炎伴不同程度滑膜炎，滑膜皱壁粘连纤维片，关节软骨不同程度破坏。镜下清除增生滑膜，松解粘连，去除剥脱软骨、修复关节面。结果随访2月～4年，平均1.5年，术后综合评估2月～1年，优良率94％；1～2年，优良率79.2％；2年以上优良率54.8％。结论关节镜对膝关节骨性关节炎诊断能提供了比较全面的资料，并对骨性关节炎早期有良好疗效，具有创伤小、恢复快、并发症少和重复治疗等优点。     

Anatomical study of distribution of valves of the cutaneous veins of adult''s limbs

Since the cutaneous veins of the four limbs have been used as autogenous grafts in the treatment of cardiovascular disorders, knowledge of distribution of the valves is increasingly required depending upon the use. In the gross anatomical study of distribution of valves of the trunci venae, there has been argument about locational relationships between the venous roots and the valves in the vicinity of the roots and the inter-valvular distance. However, there have been only few reports discussing detailed information about valves of the cutaneous veins of the four limbs. The authors observed patterns of distribution of the cutaneous venous valves of the four limbs of cadavers prepared for practice in anatomy. The following parts were excised from each cadaver: the cephalic, basilic, and the great saphenous veins, which originate from the acral venous network and flow into the proximal deep veins, and the venous roots communicating with these veins. An incision was made on each excised vein in the direction of the long axis under observation with a stereoscopic microscope, and the inter-valvular distance and the distance between the valve and the orifice of venous root in the vicinity of the valve were measured. The inter-valvular distance varied with type of the truncus venae, and it varied according to area even in the same truncus venae.     

Pathways of the realization of immunomodulating effects of neurotransmitters

Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 115, N ^o 4, pp. 401–404, April, 1993     

Characterization of sterols of three digenetic trematodes of buffalo

Sterols of three digenetic trematodes were isolated and characterized by infrared and 1H nuclear magnetic resonance spectroscopies, and gas-liquid chromatography and gas chromatography-mass spectrometry. Sterols identified were cholesterol, cholestanol, 24-methylcholesterol, 24-methylcholestanol, 24-ethyl-22-dehydrocholesterol, 24-ethyl-22-dehydrocholestanol, 24-ethylcholesterol and 24-ethylcholestanol.     

Mechanisms of generation of slow components of bioelectric activity in diagnostics of functional state of the gastrointestinal tract

New electrophysiologic devices were used for the diagnostics of the state of excitable structures in the gastrointestinal tract and correction of their motor functions. Bioelectrical and biomechanical activities form the basis of functioning of internal organs. The mechanisms of generation of slow bioelectrical activity that are important for clinical and physiological studies are described. One of these mechanisms is a capacitance parametric transducer converting the energy of contractions into specific electric signals reflecting muscle functions. Another mechanism results from slow oscillations of resting potentials of interrelated excitable cells in large neuromuscular structures of internal organs. The elaborated procedure is efficient for preventing early postoperative paresis of the gastrointestinal tract. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 128, No. 10, pp. 448–452, October, 1999     

胃小弯侧血管弓的MDCT解剖

目的：研究正常胃小弯侧血管弓及其属支血管的多排螺旋CT表现。方法：回顾性分析51例正常成人的上腹部CT资料,观察胃小弯侧血管弓及其属支血管的显示情况、血管弓形态及其与胃壁的关系。结果：51例均显示胃小弯侧血管弓,血管弓贲门部属支、胃体部属支和幽门部属支分别显示46例、15例和10例;血管弓与胃壁紧邻41例。结论：MDCT检查可良好显示正常胃小弯侧血管弓及其属支血管。     

Assessment of the efficacy of treatment of hemangiomas

Assessment of the aftereffects of cryoexposure and ultrahigh-frequency cryoexposure on hemangioma tissue of various types, cavernous and squamous, showed a higher cryogenic effect in hemangiomatous tissue preexposed to ultrahigh-frequency waves. A quantitative criterion is proposed for assessing the efficacy of the studied methods of exposure. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N ^o 6, pp. 669–672, June, 1995