芒果苷协同红霉素对痤疮致病菌抑菌作用的实验研究

目的 观察芒果苷的抑菌作用方式,为深入开发药用植物资源提供实验依据.方法 采用液体试管法培养金黄色葡萄球菌及痤疮丙酸杆菌,按试管稀释法调整细菌浓度;设置芒果苷组、芒果苷加红霉素组、红霉素组.观察各药物组对金黄色葡萄球菌及痤疮丙酸杆菌的抑菌作用.结果 芒果苷单独对金黄色葡萄球菌及痤疮丙酸杆菌的抑菌作用不明显;但其与红霉素有强烈的协同抑菌作用,100 μmol/L的芒果苷能把红霉素作用于痤疮丙酸杆菌的最小抑菌浓度(MIC)从117.43 μg/mL下降至0.117 μg/mL,有效降低红霉素的MIC浓度.结论 芒果苷与红霉素具有协同抑菌作用,两者联合使用可降低红霉素的MIC.     

棓丙酯与抗生素联合抗多重耐药鲍曼不动杆菌作用研究

目的 探讨棓丙酯与临床上常用抗生素(头孢他啶、环丙沙星和阿米卡星)对多重耐药鲍曼不动杆菌的联合抑菌作用.方法 根据临床药敏结果选取9株鲍曼不动杆菌多重耐药菌株和鲍曼不动杆菌标准菌株ATCC19606作为实验对象,用棋盘滴定法测定棓丙酯分别与头孢他啶、环丙沙星和阿米卡星的联合抑菌效应.结果 除鲍曼不动杆菌标准菌株外,9株鲍曼不动杆菌多重耐药菌株对三种抗生素皆耐药,棓丙酯对各株细菌均有一定的抑制作用,且对耐药株和标准菌株的最低抑菌浓度(MIC)基本相同;棓丙酯分别与头孢他啶、环丙沙星、阿米卡星联用后抑菌作用表现为相加.结论 棓丙酯对鲍曼不动杆菌生长具有抑制作用,与抗生素联用后抗菌活性增加.     

盐酸多西环素肠溶胶囊联合氯柳酊治疗寻常痤疮疗效观察

目的探讨盐酸多西环素联合氯柳酊治疗寻常痤疮的疗效。方法对治疗组96例寻常痤疮患者采用口服盐酸多西环素肠溶胶囊加氯柳酊外用治疗,并与对照组90例单服盐酸多西环素肠溶胶囊患者6周后进行疗效比较。结果治疗组总有效率明显优于对照组,两组比较差异有统计学意义(P<0.05)。结论盐酸多西环素肠溶胶囊口服与氯柳酊外用联合治疗,在消除痤疮的非炎症性损害和炎性损害方面有明显的协同作用,提高了临床治疗效果。     

多西环素联合30％超分子水杨酸、火针治疗丘疹脓疱型玫瑰痤疮的疗效观察

目的 评价多西环素联合外用30％超分子水杨酸、火针对丘疹脓疱型玫瑰痤疮的疗效.方法 选取丘疹脓疱型玫瑰痤疮患者100例,随机分为A、B、C组.A组行盐酸多西环素联合30％超分子水杨酸、火针治疗;B组行盐酸多西环素联合30％超分子水杨酸;C组行盐酸多西环素联合火针治疗.(盐酸多西环素100mg/d,8周后停药;超分子水杨...     

米诺环素和多西环素对表皮葡萄球菌的体外抗菌活性比较

目的观察米诺环素和多西环素对表皮葡萄球菌的体外抗菌活性。方法采用二倍琼脂稀释法对124株表皮葡萄球菌进行米诺环素和多西环素体外抗菌实验,并进行两种抗生素的抗菌效果对比。结果米诺环素和多西环素均有很好的抗菌效果,米诺环素和多西环素对81株甲氧西林敏感的表皮葡萄球菌(MSSE)的最低抑菌浓度(MIC90)分别为1 mg/L和2 mg/L。对43株耐甲氧西林表皮葡萄球菌(MRSE)的MIC90均为2 mg/L。结论表皮葡萄球菌对米诺环素和多西环素的敏感率均为100%,表明米诺环素和多西环素均有较好的抗菌效果。     

佐剂性关节炎模型大鼠滑膜组织凋亡因子Fas／FasL及Bcl-2／Bax表达与青蒿琥酯的干预

背景:类风湿关节炎的发病与细胞凋亡过程异常密切相关,其滑膜细胞过度增生源于滑膜细胞凋亡的相对不足,诱导滑膜细胞凋亡对治疗类风湿关节炎有积极意义。青蒿素及其衍生物可诱导多种细胞凋亡。目的:观察青蒿琥酯对佐剂性关节炎滑膜组织中凋亡因子Fas/FasL及Bcl-2/Bax表达的影响。设计:随机对照观察。单位:三峡大学第一临床医学院。材料:实验于2005-09/2005-11在三峡大学免疫实验室及形态动物学实验室完成,选用50只生后8周雄性Wistar大鼠,体质量(150±21)g,清洁级,由华中科技大学同济医学院动物实验中心提供。完全Freud佐剂为美国SIGMA公司产品,青蒿琥酯注射液购自桂林南药股份有限公司。兔抗鼠Fas(SC-716)、兔抗鼠FasL多抗(SC-834)、山羊抗小鼠IgG抗体-HRP多聚体、兔抗鼠P53(M3566)多抗、Bcl-2(sc-7382)多抗、兔抗鼠Bax(sc-7480)多抗以及ABC复合物试剂盒和DAB显色试剂盒均为美国SantaCruz公司产品;甲氨蝶呤由上海华联制药有限公司生产;德国Leica生物应用显微镜及图象分析系统;德国Leica切片机。方法:摸球法随机将大鼠分为6组:空白组(n=8),模型组(n=8),青蒿琥酯高剂量组(n=10),青蒿琥酯低剂量组(n=8),青蒿琥酯加甲氨蝶呤组(n=8),甲氨蝶呤组(n=8)。①实验干预:按照文献叙述的模型构建方法,除空白组外,余5组每只右足跖处给予注射0.1mL完全Freud佐剂,致炎成佐剂性关节炎模型,空白组注射生理盐水0.1mL。致炎后第13天开始给药,青蒿琥酯高剂量组、青蒿琥酯低剂量组分别注射40,20mg/(kg·d)青蒿琥酯注射液,青蒿琥酯加甲氨蝶呤组注射20mg/(kg·d)青蒿琥酯注射液,0.4mg/(kg·3d)甲氨蝶呤注射液。甲氨蝶呤组给予注射0.4mg/(kg·3d)甲氨蝶呤注射液,模型组腹腔注射1mL/d生理盐水。各组给药方式均为腹腔注射。②实验评估:给药前及给药后13d分别评价大鼠关节肿胀指数;免疫组织化学法检测给药后13d大鼠滑膜组织中Fas/FasL、Bcl-2及Bax的表达情况。主要观察指标:各组关节肿胀指数及滑膜组织中凋亡相关因子Fas/FasL,Bcl-2/Bax的表达。结果:纳入大鼠50只均进入结果分析。①给药后13d,各实验组关节肿胀指数明显低于给药前,差异有统计学意义(P<0.01),各实验组关节肿胀指数均明显低于模型组,差异有统计学意义(P<0.01)。②青蒿琥酯高剂量组、青蒿琥酯低剂量组和青蒿琥酯加甲氨蝶呤组滑膜组织中FasL及Fas均上调,与模型组比较差异有统计学意义(P<0.01),甲氨蝶呤组与模型组比较差异无统计学意义(P>0.05);青蒿琥酯高剂量组、青蒿琥酯低剂量组、青蒿琥酯加甲氨蝶呤组及甲氨蝶呤组Bcl-2表达下调,Bax上调,与模型组比较差异有统计学意义(P<0.05)。结论:实验证实青蒿琥酯具有诱导佐剂性关节炎病情缓解的作用,上调滑膜组织中Fas/FasL及Bax,下调Bcl-2的表达而诱导滑膜细胞凋亡可能是其机制之一。     

富血小板血浆对痤疮丙酸杆菌的体外抑菌实验研究

目的通过体外实验探索富血小板血浆(PRP)对痤疮丙酸杆菌的抑菌作用。方法采用纸片扩散法(简称"改良K-B法")来进行PRP体外抑菌的实验,设置红霉素溶液阳性对照药,贫血小板血浆(PPP)为阴性对照药,最后测定抑菌圈直径。结果 PRP对痤疮丙酸杆菌有较强的敏感性,在纸片扩散法实验中的抑菌圈直径为(15.37±0.747 0)mm,与PPP相比差异有统计学意义(P0.05)。参考抗生素药敏实验等级划分的判断标准,PRP对痤疮丙酸杆菌的抑菌效果属于高敏。结论 PRP对痊疮的主要致病菌座疮丙酸杆菌有良好的抑菌效果。     

磺胺类药物对结核分枝杆菌体外抗菌活性的研究

目的评价磺胺甲噁唑(SMZ)和甲氧苄啶(TMP)对结核分枝杆菌(MTB)的体外抑菌作用,为临床应用提供依据。方法应用微孔板观察法,测定TMP/SMZ对MTB敏感标准株H37Rv、临床敏感菌株(20株)和临床耐多药(MDR)菌株(30株)的最小抑菌浓度(MIC)。结果标准株H37Rv的MIC值为0.5μg/mL TMP+9.5μg/mL SMZ。40株(80%)临床分离MTB可以被MIC值为1μg/mL TMP+19μg/mL SMZ的复合制剂抑制生长,其中包含17株敏感菌株、23株MDR菌株。结论 TMP联合SMZ对MTB菌株有较好的体外抑菌活性。     

青蒿琥酯通过调控巨噬细胞移动抑制因子对重症肺炎大鼠炎症反应的影响研究北大核心CSCD

目的研究青蒿琥酯通过调控巨噬细胞移动抑制因子（MIF）影响重症肺炎大鼠的炎症反应程度。方法100只sD大鼠随机（随机数字法）分组：其中20只sD为对照组,另80只sD大鼠建立肺炎克雷伯杆菌肺炎大鼠模型,其中20只为模型组,另60只以不同浓度的青蒿琥酯（20,40,80mk／kg）处理大鼠,5天后观察大鼠组织病理、巨噬细胞移动抑制因子的表达、髓过氧化物酶活性和炎性细胞浸润等指标变化。结果青蒿琥酯治疗后显著减弱重症肺炎大鼠的炎症严重程度,包括髓过氧化物酶活性[重症肺炎组：（17．5±1．5）;治疗组：（7．5±2．0）]和炎性细胞浸润的减少（重症肺炎组：27×10^6;治疗组：12．5×10^6）。青蒿琥酯治疗也显著减少支气管肺泡灌洗液中炎症细胞因子的产生[IL—1重症肺炎组：（1100±50）pg／mL;治疗组：（400±60）pg／mL;IL-6重症肺炎组：（700±30）pg／mL;治疗组：（200±40）pg／mL;IL-10重症肺炎组：（500±70）pg／mL;治疗组：（200±40）pg／mL;TNF—α重症肺炎组：（500±80）pg／mL;治疗组：（150±50）pg／mL]。此外,青蒿琥酯能抑制MIF的表达和产生,从而抑制由MIF介导的炎症反应。结论青蒿琥酯通过抑制MIF介导的炎症反应,对肺炎克雷伯杆菌肺炎大鼠具有保护作用,这为肺炎克雷伯杆菌肺炎大鼠中新的药物治疗提供了分子基础。     

青蒿琥酯诱导人急性白血病原代细胞凋亡、胀亡的作用机制研究

目的:探讨青蒿琥酯诱导人急性白血病(AL)原代细胞凋亡、胀亡作用的可能机制。方法:提取11例急性白血病患者骨髓液进行原代细胞培养,以对数生长期细胞为干预点,将不同浓度青蒿琥酯作用于细胞,分别采用四甲基偶氮唑盐(MTT)法检测青蒿琥酯对人AL原代细胞增殖的影响;透射电子显微镜观察青蒿琥酯作用后细胞的形态变化;免疫印迹法检测凋亡相关蛋白。结果:与对照组比较,青蒿琥酯对人AL骨髓原代培养细胞有抑制作用;青蒿琥酯具有诱导人AL骨髓原代细胞凋亡和胀亡的作用,青蒿琥酯可下调凋亡抑制蛋白Bcl-2含量,同时促进凋亡蛋白Bax、细胞色素C的释放。结论:青蒿琥酯可诱导人AL原代细胞凋亡和胀亡,线粒体途径可能是青蒿琥酯诱导白血病细胞死亡的主要途径。     

In vitro activities of cefotaxime, vancomycin, quinupristin/dalfopristin, linezolid and other antibiotics alone and in combination against Propionibacterium acnes isolates from central nervous system infections

OBJECTIVES: To evaluate the antibiotic susceptibilities of Propionibacterium acnes isolates from central nervous system (CNS) infections to agents used in current treatment regimens. METHODS: MICs of 16 reference antibiotics were determined by an agar dilution method for 24 consecutive strains of P. acnes isolated from individual patients with intracranial empyema or brain abscess. Bactericidal activities of antibiotics against P. acnes PAN14 were studied at 0.25-2 x MIC using a time-kill method. RESULTS: All of the isolates were resistant to fosfomycin, intermediate or resistant to metronidazole and susceptible to all the other antibiotics tested, except for nine strains, which were intermediate to ofloxacin. Among antibiotics tested alone in time-kill experiments, vancomycin was the most effective drug and exhibited bactericidal activity after 24 h at 1x and 2 x MIC, whereas cefotaxime and ciprofloxacin were bactericidal after 48 h at 2 x MIC. No significant bactericidal activity could be demonstrated with the other antibiotics tested alone. The addition of cefotaxime to vancomycin resulted in bactericidal activity at lower concentrations (0.5 x MIC), whereas synergy was observed between quinupristin/dalfopristin and cefotaxime at 2 x MIC. In contrast, antagonism was observed between cefotaxime and linezolid, and ciprofloxacin and clindamycin. CONCLUSION: Our data suggest that P. acnes isolates causing CNS infections remain highly susceptible to most antibiotics used for the treatment of such infections. Moreover, we showed that cefotaxime, vancomycin and ciprofloxacin possess good bactericidal activities against P. acnes, and that these activities may be enhanced when vancomycin is combined with cefotaxime or when cefotaxime is combined with quinupristin/dalfopristin.     

Artesunate and artemether are effective fasciolicides in the rat model and in vitro

OBJECTIVES: To study the fasciocidal properties of artesunate and artemether in the rat model and in vitro. METHODS: Adult Fasciola hepatica were exposed in vitro to 1, 10 and 100 microg/mL of artesunate, artemether and dihydroartemisinin for 72 h. Female Wistar rats were administered a single oral dose of artesunate and artemether (100-400 mg/kg) commencing 3 or 10-14 weeks post-infection and worm burden reductions were assessed against infected but untreated control rats. F. hepatica were also observed by scanning electron microscopy (SEM) after recovery from bile ducts of rats given a single oral dose of 200 mg/kg artesunate 24 and 72 h post-treatment. RESULTS: F. hepatica exposed for 72 h to 10 microg/mL of artesunate, artemether and dihydroartemisinin in vitro showed poor mobility, swelling of the worm body, roughness, damage of the tegument and blebbing. Exposure to drug concentrations of 100 microg/mL resulted in the death of all F. hepatica by 72 h. One hundred per cent worm burden reductions were achieved in rats infected with adult F. hepatica after treatment with artesunate and artemether at 400 and 200 mg/kg, respectively. Administration of artesunate and artemether at a dose of 200 mg/kg to rats harbouring juvenile F. hepatica resulted in worm burden reductions of 46% and 82%, respectively. F. hepatica recovered from rats' bile ducts 24 h after administration of 200 mg/kg artesunate showed normal activity and SEM observations revealed that there was no visible damage. Seventy-two hours post-treatment F. hepatica displayed very poor mobility and there was focal swelling of the tegument and spines. CONCLUSIONS: Artesunate and artemether exhibit promising fasciocidal activities, with the latter showing better tolerability by the hosts.     

黄连中药根碱分别联合阿米卡星与头孢哌酮/舒巴坦对泛耐药鲍曼不动杆菌的体外抑菌作用

目的探讨黄连中药根碱分别联合阿米卡星与头孢哌酮/舒巴坦2种抗菌药物对泛耐药鲍曼不动杆菌的抑菌作用。方法应用肉汤稀释法测定不同浓度组合黄连中药根碱与阿米卡星、头孢哌酮/舒巴坦对25株临床分离泛耐药鲍曼不动杆菌的最低抑菌浓度(MIC)及分级抑菌浓度(FIC)指数,并观察其对25株泛耐药鲍曼不动杆菌的体外抑菌效果。结果黄连中药根碱分别联合阿米卡星、头孢哌酮/舒巴坦后,其MIC范围与3药单独相比保持不变或有所降低,而黄连中药根碱联合头孢哌酮/舒巴坦的MIC及分布浓度均明显降低,差异有统计学意义(P<0.05)。在累计抑菌率相同时,联合用药的药物浓度较单独用药时低,其中黄连中药根碱联合头孢哌酮/舒巴坦的药物浓度降低最明显,差异有统计学意义(P<0.05)。黄连中药根碱联合阿米卡星用药的FIC指数为1.0~<2.0时占比为72%,以无关作用为主;黄连中药根碱联合头孢哌酮/舒巴坦用药的FIC指数为0.5~<1.0时占比为72%,以相加作用为主。结论黄连中药根碱联合头孢哌酮/舒巴坦增强了单独用药(头孢哌酮/舒巴坦)的抑菌效果,通过减少头孢哌酮/舒巴坦的用量可以延缓细菌耐药。     

In vitro antibacterial activity of NB-003 against Propionibacterium acnes

NB-003 and NB-003 gel formulations are oil-in-water nanoemulsions designed for use in bacterial infections. In vitro susceptibility of Propionibacterium acnes to NB-003 formulations and comparator drugs was evaluated. Both NB-003 formulations were bactericidal against all P. acnes isolates, including those that were erythromycin, clindamycin, and/or tetracycline resistant. In the absence of sebum, the MIC(90)s/minimum bactericidal concentrations (MBC(90)s) for NB-003, NB-003 gel, salicylic acid (SA), and benzoyl peroxide (BPO) were 0.5/2.0, 1.0/2.0, 1,000/2,000, and 50/200 μg/ml, respectively. In the presence of 50% sebum, the MIC(90)s/MBC(90)s of NB003 and BPOs increased to 128/1,024 and 400/1,600 μg/ml, respectively. The MIC(90)s/MBC(90)s of SA were not significantly impacted by the presence of sebum. A reduction in the MBC(90)s for NB-003 and BPO was observed when 2% SA or 0.5% BPO was integrated into the formulation, resulting in MIC(90)s/MBC(90)s of 128/256 μg/ml for NB003 and 214/428 μg/ml for BPO. The addition of EDTA enhanced the in vitro efficacy of 0.5% NB-003 in the presence or absence of 25% sebum. The addition of 5 mM EDTA to each well of the microtiter plate resulted in a >16- and >256-fold decrease in MIC(90) and MBC(90), yielding a more potent MIC(90)/MBC(90) of ≤1/<1 μg/ml. The kinetics of bactericidal activity of NB-003 against P. acnes were compared to those of a commercially available product of BPO. Electron micrographs of P. acnes treated with NB-003 showed complete disruption of bacteria. Assessment of spontaneous resistance of P. acnes revealed no stably resistant mutant strains.     

青蒿琥酯对生存素在乳腺癌细胞株MDA-MB-231表达的影响

目的 探讨青蒿琥酯对生存素(survivin)在乳腺癌细胞株MDA-MB-231中表达的影响.方法 以不同浓度(0、25、50 μg/mL)的青蒿琥酯处理MDA-MB-231细胞,分别于药物处理后24、48和72 h收集细胞及培养上清液,用RT-PCR检测不同组别、不同时间点的细胞中survivin的基因表达情况,酶联免疫吸附试验(ELISA)检测各组、各时间点培养上清液中survivin的蛋白质表达水平.结果 PCR结果和ELISA结果均显示,随着药物浓度的增加和处理时间的延长,survivin基因水平和蛋白质水平的表达也逐渐降低,同一时间点各组间survivin的表达量进行单因素方差分析,差异有统计学意义(P＜0.05);对照组(0 μg/mL)在24 h和48 h survivin的表达量变化不大,经t检验分析,差异无统计学意义(P＞0.05),而各浓度经药物处理48 h后survivin的表达量明显低于24 h的表达,差异有统计学意义(P＜0.05).结论 青蒿琥酯可通过抑制survivin的表达促进乳腺癌MDA-MB-231细胞凋亡.     

Role of lipids in augmenting the antibacterial activity of benzoyl peroxide against Propionibacterium acnes.

Antimicrobial activity and physicochemical properties of benzoyl peroxide (BP) were investigated to determine the mechanism of action for the compound as an antiacne agent. The MICs and MBCs against nine strains of Propionibacterium acnes ranged between 100 and 800 micrograms/ml in a nutrient broth system, with a median fourfold increase in activity demonstrated when lipid was added. The partition coefficient of BP in a 50:50 artificial skin lipid and water system was greater than 2,500, with the concentration of BP soluble in lipid measured at 1.12% and in water at 0.005%. When BP was incubated in the presence of a lipid mixture, reaction products were formed, with evidence that at least some of these compounds possessed antibacterial activity. These results suggest that BP reduces the P. acnes numbers in sebaceous follicles because of good lipid solubility and interaction with the lipid component, the latter property contributing to the antimicrobial activity of the compound in a high-lipid environment.     

Successful management of central nervous system infection due to Propionibacterium acnes with vancomycin and doxycycline

Propionibacterium acnes is an infrequent but increasingly recognized cause of neurosurgical infections. In this setting, it has been most commonly reported as complicating neurosurgical shunt procedures. The optimum therapy for central nervous system infections caused by P. acnes has not been established. The authors report on a patient who had development of P. acnes central nervous system infection after craniotomy for subdural hematoma. This case was successfully treated with prolonged therapy with vancomycin and doxycycline. Neurologic symptom improvement and radiographic resolution were documented on this regimen, and neurosurgical intervention was not required.     

Activity of nadifloxacin (OPC-7251) and seven other antimicrobial agents against aerobic and anaerobic Gram-positive bacteria isolated from bacterial skin infections

BACKGROUND AND METHODS: The in vitro activity of nadifloxacin (OPC-7251), a novel topical fluoroquinolone, was assessed and compared with those of ofloxacin, oxacillin, flucloxacillin, cefotiam, erythromycin, clindamycin, and gentamicin against 144 Gram-positive bacteria: 28 Staphylococcus aureus, 10 Streptococcus spp., 68 coagulase-negative staphylococci (CNS), 36 Propionibacterium acnes, and 2 Propionibacterium granulosum strains. All strains originated from bacterial-infected skin disease and were isolated from patients with impetigo, secondary infected wounds, folliculitis and sycosis vulgaris, and impetiginized dermatitis. In vitro susceptibility of all clinical isolates was tested by agar dilution procedure and minimum inhibitory concentrations (MICs) were determined. RESULTS: Nadifloxacin was active against all aerobic and anaerobic isolates. MIC(90) (MIC at which 90% of the isolates are inhibited) was 0.1 microg/ml for S. aureus, 0.78 microg/ml for both Streptococcus spp. and CNS, and 0.39 microg/ml for Propionibacterium spp. On the other hand, resistant strains with MICs exceeding 12.5 mug/ml were found in tests with the other antibiotics. For both CNS and Propionibacterium acnes, MIC(90) values > or =100 microg/ml were demonstrated for erythromycin. Ofloxacin, cefotiam, erythromycin, clindamycin and gentamicin exhibited MIC(90) values < or =1 microg/ml for some bacterial species tested. Both oxacillin and flucloxacillin were active against all investigated bacterial species with MIC(90) values < or =1 microg/ml. CONCLUSION: In summary, nadifloxacin, a topical fluoroquinolone, was found to be highly active against aerobic and anaerobic bacteria isolated from patients with infected skin disease, and seems to be a new alternative for topical antibiotic treatment in bacterial skin infections.     

Suppression of polymorphonuclear leukocyte chemotactic factor production in Propionibacterium acnes by subminimal inhibitory concentrations of tetracycline, ampicillin, minocycline, and erythromycin.

Propionibacterium acnes is the cause of inflammation in acne vulgaris and has been shown to produce potent neutrophil chemoattractants. Different strain of P. acnes that were sensitive or resistant to ampicillin, erythromycin, minocycline, and tetracycline were grown in the presence of subminimal inhibitory concentrations of the drugs, and their culture supernatants were assayed for neutrophil chemotactic activity. The presence of subminimal inhibitory concentrations of ampicillin failed to affect chemotactic factor production by any of the strains. Subminimal inhibitory concentrations of tetracycline, minocycline, and erythromycin all produced decreased neutrophil chemotactic activity in P. acnes culture supernatants. This inhibition of chemotactic activity was most pronounced in strains of P. acnes which were susceptible to the drugs. The addition of antibiotics at appropriate concentrations to control supernatants failed to affect neutrophil migration. The results indicate that subminimal inhibitory concentrations of antibiotics are capable of reducing the inflammatory capacity of P. acnes.