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1.
目的 探讨肝硬化患者血清一氧化氮 (NO)、白细胞介素 2 (IL 2 )、白细胞介素 6 (IL 6 )、白细胞介素 8(IL 8)及可溶性白细胞介素 2受体 (sIL 2R)变化的临床意义。方法 应用ELISA法测定肝硬化患者及正常对照组的血清IL 6、IL 8及sIL 2R含量 ;应用MTT法检测血IL 2活性 ;应用荧光法检测血清NO水平。结果 肝硬化患者血清IL 2、sIL 2R、IL 6、IL 8及NO水平 :(5 741.5 3± 4376 .5 2 )U/ml、(486 .76± 46 .41)U/ml、(15 .78± 3.0 4) pg/ml、(2 3.89± 2 .13)pg/ml及 (6 .33± 0 .37) μmol/L ,显著高于正常对照组 :(173.88± 92 .2 1)U/ml、(2 42 .36± 35 .78)U/ml、(6 .14± 3.12 ) pg/ml、(17.71± 1.32 )pg/ml及 (3.6 8± 0 .34 ) μmol/L ,并随肝功受损程度进行性增加。 结论 肝硬化患者血清IL 2、sIL 2R、IL 6及IL 8增加可能为NO增多的诱发因素 ;肝功能损伤可能是白细胞介素活性增加的重要原因。  相似文献   

2.
目的 通过观察黄芪对慢性肾小球肾炎患者白细胞介素 2 /白细胞介素 2受体 (IL 2 /IL 2R)系统的影响 ,探讨黄芪对慢性肾炎的免疫调节机理。方法  42例慢性肾炎患者随机分为治疗组 (12例 ,黄芪注射液 60ml + 5 %葡萄糖注射液 5 0 0ml,静脉点滴 ,1次 /d ,疗程为一个月 ) ;对照组 (2 0例 ,除不给黄芪外 ,其它治疗与治疗组相同 )。测定治疗前后血清IL 2和血清、淋巴细胞培养上清液中的sIL 2R。结果  (1)与正常组比较 ,慢性肾炎患者血清IL 2显著降低 ,血清和淋巴细胞培养上清液中的sIL 2R显著增高 (P <0 .0 0 1) ;(2 )治疗后 ,治疗组血清IL 2明显升高 ,血清和淋巴细胞培养上清液中sIL 2R明显降低 ,与治疗前及对照组治疗后比较 ,差异有显著性 (P <0 .0 0 1) ;(3 )对照组治疗前、后血清IL 2、血清和淋巴细胞培养上清液中sIL 2R水平无明显改变 (P >0 .0 5 )。结论 黄芪能有效调节、改善慢性肾炎患者的免疫功能 ,可作为慢性肾炎的一种有效、安全、价廉的免疫调节剂  相似文献   

3.
目的 探讨阿尔茨海默病 (AD)的病因及发病机制。方法 采用双抗体夹心酶联免疫吸附试验分别对AD患者血清和脑脊液中白细胞介素 2 (IL 2 )及白细胞介素 2受体 (sIL 2R)水平进行检测。结果  (1)AD组脑脊液 (CSF)中sIL 2R阳性率明显高于脑血管性痴呆 (VD)组及对照组 (P <0 .0 5 ) ;(2 )AD组血清和CSF中IL 2和sIL 2R含量明显高于VD组和正常对照组 (P <0 .0 5 ,P <0 .0 1) ;(3)AD组IL 2与sIL 2R在血清或CSF中含量均呈正相关 ,与简易智力状态检查量表得分呈负相关。结论 AD组患者CSF中IL 2和sIL 2R可来源于外周免疫系统 ,还可能来源于中枢神经系统的自身合成。检测AD患者CSF中IL 2和sIL 2R水平可反映AD的病理改变程度  相似文献   

4.
目的 探讨α 干扰素 (INF α 1b)治疗慢性乙型肝炎细胞免疫机制。方法 收集IFN α 1b治疗慢性乙型肝炎患者 3 0例 ,分别在治疗前、治疗后 1个月、3个月、6个月和 9个月留取血清标本 ,用MTT法检测血清白细胞介素 1(IL 1)水平 ,用ELISA法检测血清可溶性白细胞介素 2受体 (sIL 2R)和γ 干扰素 (INF γ)水平 ,并与 3 0例健康献血员作对照。结果 与正常对照组相比 ,慢性乙型肝炎患者血清sIL 2R水平升高 (P <0 0 5 ) ,经IFN α治疗后 ,血清IL 1、sIL 2R明显升高 (P <0 0 1)。结论 IFN α 1b调节体内细胞因子变化 ,参与细胞免疫调节。  相似文献   

5.
目的 :观察充血性心力衰竭 ( CHF)患者血清白细胞介素 6( IL- 6)和可溶性白细胞介素 6受体 ( s IL-6R)水平的变化 ,探讨其与 CHF患者的病因和心功能的关系。方法 :用 EL ISA法检测 5 3例治疗前的 CHF患者( CHF组 )血清中 IL - 6和 s IL - 6R水平 ,并和 2 0例健康者 (对照组 )比较。结果 :CHF组血清 IL - 6和 s IL - 6R水平显著高于对照组 ( P <0 .0 1) ,且随心功能不全的恶化逐渐升高 ,心功能 ~ 级各亚组间差异有非常显著性意义( P <0 .0 1)。 IL - 6和 s IL - 6R水平分别与左室射血分数呈显著负相关 ( r =- 0 .81,P <0 .0 1和 r=- 0 .83 ,P <0 .0 1) ,s IL - 6R和 IL - 6水平呈显著正相关 ( r =0 .91,P <0 .0 1)。不同病因组间的 IL - 6及 s IL - 6R水平差异无显著性意义。结论 :CHF患者血清 IL- 6及 s IL- 6R显著升高 ,且与心力衰竭程度明显相关 ,与心力衰竭病因无关。测定 IL- 6和 s IL- 6R水平可作为 CHF诊断的实验指标 ,提示 IL- 6及 s IL- 6R参与 CHF的发生、发展过程  相似文献   

6.
糖尿病血瘀证病人血清IL-6和sIL2R水平的研究   总被引:2,自引:0,他引:2  
目的研究糖尿病血瘀证病人IL 6、sIL2R水平的变化,探讨糖尿病血瘀证同IL 6、sIL2R的关系.方法将40例2型糖尿病血瘀证病人与35例非血瘀证病人的IL 6、sIL2R以及糖代谢相关指标进行比较,并以25例成人健康者作对照.应用化学发光法检测血清IL 6、sIL2R.结果同健康对照组相比,糖尿病血瘀证和非血瘀证病人血清IL 6水平明显升高(P<0.01),sIL2R水平未见明显变化.其中,血瘀证病人血清IL-6水平明显高于非血瘀证组(P<0.01).结论糖尿病时,炎症反应可能参与了血瘀证的形成,血清IL-6水平升高可能是糖尿病血瘀证的病理生理之一.  相似文献   

7.
目的 探讨可溶性白介素 2受体 (sIL 2R)、白介素 6 (IL 6 )在急性淋巴细胞性白血病(ALL)的脑脊液 (CSF)中的表达及意义。方法 用双抗夹心ELISA法测定 30例ALL患者CSF中sIL 2R、IL 6水平 ,并与 10名正常者进行对照。结果 ALL合并中枢神经系统白血病 (CNS L)组较CNS L已缓解及未合并CNS L组二者水平显著升高 (P <0 .0 1) ;CNS L已缓解组二者水平接近对照组 (P >0 .0 5 ) ;骨髓缓解及好转组二者水平低于治疗无效组。结论 监测二者水平变化有助于CNS L早期诊断、评估疗效及预后。  相似文献   

8.
目的 :观察茶碱对哮喘患者血清T淋巴细胞活性影响。方法 :检测哮喘患者每d给予茶碱控释片 4 0 0mg治疗 6周前后血清可溶性白细胞介素 2受体 (sIL 2R)浓度、T淋巴细胞亚群和IgE水平变化。结果 :哮喘患者经茶碱治疗 6周后其血清sIL 2R浓度显著下降 ,从治疗前 ( 0 31± 0 10 )U/L ,降低到治疗后 ( 0 2 5± 0 0 6 )U/L ,P <0 0 5。茶碱能显著提高哮喘患者外周血中的CD8T淋巴细胞数 (从治疗前的 18 9%± 4 4 %提高到治疗后的 2 5 8%± 5 9% ,P <0 0 5 ) ,同时CD4 /CD8%相应地显著降低 (P <0 0 5 )。茶碱并有降低哮喘患者外周血中IgE水平的趋势。结论 :小剂量茶碱能抑制哮喘患者血清T淋巴细胞活性 ,不仅具有抗炎作用 ,还有免疫调节作用 ,为评价茶碱在哮喘治疗中的作用提供了依据。  相似文献   

9.
老年慢性阻塞性肺疾病血清白细胞介素-13的变化   总被引:2,自引:0,他引:2  
目的 探讨白细胞介素 13 (IL 13 )与慢性阻塞性肺疾病 (COPD )的关系。方法 用双抗夹心ABC ELISA法测定 3 5例老年COPD患者 (急性加重期 2 0例 ,稳定期 15例 )血清IL 13水平 ,并与 2 2名健康者 (对照组 )进行比较。结果 老年COPD急性加重期组 ,COPD稳定期组和对照组血清IL 13水平分别为 (4 7.2 6± 3 .47) pg/ml、(3 6.11± 5 .3 4)pg/ml和 (3 0 .99± 2 .19) pg/ml。COPD急性加重期组和COPD稳定期组血清IL 13水平均显著高于对照组 (P <0 .0 5 ) ,且COPD急性加重期组血清IL 13水平显著高于COPD稳定期组 (P <0 .0 5 )。结论 老年COPD患者血清中IL 13水平显著升高 ,IL 13与COPD的发生发展有关  相似文献   

10.
目的 为探讨泡球蚴病患者经阿苯达唑治疗后 ,机体免疫应答的状态和对病程转归影响。方法  35例泡球蚴病患者经阿苯达唑治疗 1 2月前后 ,血清中sIL - 2R ,IFN -γ ,IL - 4IL - 5 ,IL - 6 ,TNFα,及特异性IgG ,IgE抗体进行检测。 结果 在治疗 1 2月后 ,Th1型细胞因子sIL - 2R ,IFN -γ无改变 (P >0 0 5) ,Th2型细胞因子IL - 4显著性下降 (P <0 0 1 ) ,IL - 5在患者中检出率明显降低 (P <0 0 1 ) ,TNFα和IgE水平较治疗前显著性下降 (P <0 0 1 )。 结论 泡球蚴病患者治疗后 ,Th2型细胞因子反应减弱 ,机体的保护性免疫应答有所恢复  相似文献   

11.
The immunoneuroendocrine role of melatonin   总被引:19,自引:0,他引:19  
Abstract: A tight, physiological link between the pineal gland and the immune system is emerging from a series of experimental studies. This link might reflect the evolutionary connection between self-recognition and reproduction. Pinealectomy or other experimental methods which inhibit melatonin synthesis and secretion induce a state of immunodepression which is counteracted by melatonin. In general, melatonin seems to have an immunoenhancing effect that is particularly apparent in immunodepressive states. The negative effect of acute stress or immunosuppressive pharmacological treatments on various immune parameters are counteracted by melatonin. It seems important to note that one of the main targets of melatonin is the thymus, i.e., the central organ of the immune system. The clinical use of melatonin as an immunotherapeutic agent seems promising in primary and secondary immunodeficiencies as well as in cancer immunotherapy. The immunoenhancing action of melatonin seems to be mediated by T-helper cell-derived opioid peptides as well as by lymphokines and, perhaps, by pituitary hormones. Melatonin-induced-immuno-opioids (MHO) and lymphokines imply the presence of specific binding sites or melatonin receptors on cells of the immune system. On the other hand, lymphokines such as -γ-interferon and interleukin-2 as well as thymic hormones can modulate the synthesis of melatonin in the pineal gland. The pineal gland might thus be viewed as the crux of a sophisticated immunoneuroendocrine network which functions as an unconscious, diffuse sensory organ.  相似文献   

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Abstract: The abundance of gap junctions between rat pineal astrocytes formed by connexin43 (Cx43) was studied during development. Levels and distribution of Cx43 were measured by immunoblotting and indirect immunofluorescence, respectively. The amount of Cx43 in cells located within the gland was low until about the 7th postnatal day and increased to adult values between the 14th and 21st days postpartum. Although astrocytes, recognized by their vimentin immunoreactivity, were scarce before birth, they were abundant by the 7th postnatal day suggesting that the low levels of Cx43 found at this age corresponded to a low expression of this protein. Localization of the immunoreactivity to Cx43 and vimentin showed a close correlation, indicating that mature or immature pineal astrocytes form gap junctions made of Cx43. Since Cx43 levels attained their adult values at about the time the innervation and the functional state of the gland reached maturity (2–3 weeks after birth), it is proposed that astrocyte gap junctions are involved in the function of the adult rat pineal gland.  相似文献   

14.
Abstract: Herein we documented the response of pineal melatonin production to electrolytes known to be effective on pineal function in view of a possible circadian stage dependence. We studied the release of melatonin by perifused rat pineal glands at 2 different circadian stages corresponding to the middle of the light and dark periods, i.e., respectively, 7 and 19 HALO (Hours After Light Onset, L:D = 12:12). The initial efflux rates were, as expected, much higher in the perifusates of glands removed from rats sacrificed during the dark phase than of those removed during the light phase. After 3 hr of perifusion, melatonin release reached similar levels which were found constant up to the 8th hr of perifusion, whatever the circadian stage. Perifusion of the glands with physiological concentrations for the rat of calcium (5.2 mmol/1) and magnesium (1.34 mmol/1) resulted in a stimulatory effect on the pineal glands removed from rats sacrificed in the middle of the dark period (19 HALO), whereas no effects were observed on the pineal glands removed from rats sacrificed during the light (7 HALO). Lithium (0.28 and 0.55 mmol/1) was ineffective on melatonin release in pineal glands removed 7 and 19 HALO. Our results show differences in the initial efflux rates of melatonin and in the response of perifused pineal glands to calcium and magnesium according to the circadian stage.  相似文献   

15.
Duodenal diverticula are a relatively common condition. They are asymptomatic, unless they become complicated, with perforation being the rarest but most severe complication. Surgical treatment is the most frequently performed approach. We report the case of a patient with a perforated duodenal diverticulum, which was diagnosed early and treated conservatively with antibiotics and percutaneous drainage of secondary retroperitoneal abscesses. We suggest this method could be an acceptable option for the management of similar cases, provided that the patient is in good general condition and without septic signs.  相似文献   

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Abstract: The use of antisera raised against bovine growth hormone (GH) and ovine prolactin (PRL) enabled the detection of related immunoreactive (ir) sequences of proteins in ovine pineal tissue. The isolation of PRL-like ir-material was accomplished using a 0.25 M ammonium sulphate (pH 5.5) extraction followed by ethanol precipitation, whereas the resulting 2.0 M ammonium sulphate (pH 7.0) precipitate contained a GH-like immunoreactivity. Gel chromatography of the GH-like immunoreactivity (Sephadex G-100) indicated the presence of several GH-like fragments ranging in the Mr range of 7,000 to 55,000. Analyses of the PRL-like ir-material found in pineal tissue on HPLC using a TSK 545-DEAE column led to the resolution into a single peak of immunoreactivity. A single peak of activity was also observed following chromatofocusing and hydrophobic interaction chromatography of the ir-peak from the TSK 545-DEAE column. The PRL-like ir-material inhibited the binding of [125I]ovine PRL-S14 to anti-ovine PRL antibodies without showing an affinity for binding to anti-rat PRL or anti-bovine GH antibodies. Scatchard analysis of the binding of pineal PRL-like ir-material and pituitary ovine PRL-S14 to liver membranes from day-20 pregnant rats revealed similar affinity constants (Ka of 4.7 ± 0.2 × 109 M-1). In addition, the replication of Nb 2 Node rat lymphoma cells was stimulated by pineal PRL-like ir-material, an effect known to be specific for lactogenic hormones. The pineal PRL-like immunoreactivity appeared on sodium dodecyl sulfate polyacrylamide gels as a single major band of Mr 24,000. The functional status of PRL-and GH-like ir-material in the ovine pineal remains to be determined, but evidence is presented that the overall protein synthesis rate of the rat pineal responded to circulating concentrations of PRL.  相似文献   

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PURPOSE: Individuals who are seropositive for the human immunodeficiency virus are at high risk for opportunistic infection and anorectal disorders. Little prospective information is available regarding anorectal pathogens in these patients. METHODS: One hundred sixty-three HIV-seropositive patients presented to the colorectal clinic between 1989 and 1992. Forty-seven (29 percent) patients were thought to have an infectious process and were prospectively studied using a standardized multiculture protocol. RESULTS: Mean age was 33 (range, 19–59) years. All were male; high-risk behavior accounted for 87 percent of HIV transmissions. Presenting complaints included anorectal pain (79 percent), pus per anum (28 percent), and blood per anum (26 percent). Examination revealed perianal tenderness (60 percent), condyloma (38 percent), perianal ulcers (38 percent), and anal fissures (34 percent). Sixty-six sets of cultures were performed; 28 patients had one set, 15 had two sets, and 4 had three sets. Thirty-two of these 47 patients (68 percent) had positive cultures including herpes (50 percent), cytomegalovirus (25 percent),Neisseria gonorrhoeae (16 percent), chlamydia (16 percent), acidfast bacilli (2 percent), and others (9 percent). Six of 32 patients with positive cultures had more than one organism cultured. Sixteen (50 percent) patients with positive cultures were treated medically, 8 (25 percent) were treated surgically and 8 (25 percent) were treated with both modalities. Sixty-one procedures were performed on 17 patients for condylomata. Eighteen patients had 20 procedures for abscesses, 50 percent of whom had positive cultures for other than common bowel flora; all improved. Fourteen patients underwent 33 procedures for perianal fistulas.Mycobacterium fortuitum was cultured from one patient who required 13 procedures for abscesses and fistulas. Forty-five (96 percent) patients were followed for an average of 12.5 months ±2.9 SEM (range, 1–94 months). Symptoms were improved or resolved in 22 of 32 (69 percent) patients with positive cultures and in 11 of 13 (84 percent) with negative cultures. CONCLUSIONS: Specific pathogens may often be identified in human immunodeficiency virus-seropositive patients with anorectal disorders if aggressively sought. Although patients without specific pathogens identified may be expected to improve with planned empiric treatment, positive identification allows more directed therapy.  相似文献   

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