支气管哮喘与PBMC上CD11a、CD18、CD44分子表达的关系

本文探讨 β整合素家族及CD44粘附分子在哮喘发病中的作用。采用流式细胞仪分别检测哮喘豚鼠外周血单个核细胞(PBMC )表面CD11a分子及哮喘患者PBMC表面CD18和CD44等分子的表达情况。结果 :(1)哮喘豚鼠PBMC表面CD11a分子表达明显高于对照组 (P <0 0 1) ,地塞米松可抑制CD11a表达 ;(2 )哮喘患者PBMC表面CD18、CD44表达较正常人明显增高 (P <0 0 1)。使细胞粘附分子参与了哮喘的病理生理过程 ,糖皮质激素可抑制细胞粘附分子表达 ,发挥抗炎作用。     

细胞粘附分子与支气管哮喘

粘附分子(Adhesion molecules,AMS)是一类介导细胞间粘附及细胞与细胞外基质粘附的糖蛋白,传递细胞间信息,调节细胞功能,在炎性细胞向气道炎区跨内皮转移和浸润中具有重要作用,是支气管哮喘发病机制中的重要活性物质[1],深入研究两者间的关系将为揭示哮喘的发病机制和制定相应的治疗措施提供理论基础.     

粘附分子与哮喘

粘附分子与哮喘苗会（中日友好临床医学研究所北京１０００２９）哮喘是一种复杂的炎性疾病，呼吸道粘膜炎性细胞浸润是其特点之一。浸润的炎性细胞包括嗜酸细胞、中性粒细胞、嗜碱细胞及淋巴细胞等。这些炎性细胞释放的炎症介质，包括碱性蛋白质（ｂａｓｉｃｐｒｏｔｅｉ...     

支气管哮喘患者粘附分子和细胞因子的变化及其意义

目的:探讨支气管哮喘患者外周血白细胞粘附分子β2整合素(β2-integrin熏CD18)、血清可溶性细胞间粘附分子-1(sICAM-1,CD54)、白细胞介素-6(IL-6)和白细胞介素-8(IL-8)的变化及其意义。方法:采用流式细胞仪技术检测外周血白细胞CD18和血清sICAM-1的表达;采用酶连接免疫吸附方法(ELISA)检测血清IL-6和IL-8水平。结果:(1)与正常对照组相比,支气管哮喘患者外周血白细胞CD18和血清sICAM-1表达显著增加穴P<0.05雪;(2)与正常对照组相比,支气管哮喘患者血清IL-6和IL-8水平显著增加穴P<0.05雪;(3)支气管哮喘患者外周血白细胞CD18和血清sICAM-1表达呈显著正相关(r=0.791熏P<0.05)。结论:支气管哮喘患者外周血白细胞CD18和血清sICAM-1表达以及IL-6和IL-8水平增加,可能是支气管哮喘重要的发病机制之一。     

粘附分子与哮喘气道炎症细胞的迁移

近年研究提出，粘附分子作为前炎症物质在支气管哮喘发病中起重要的作用，本文就粘附分子在哮喘气道炎症细胞的迁移，特别是选择性地介导嗜酸性，嗜碱性粒细胞和Ｔ细胞跨内皮迁移，以及粘附分子在哮喘中的研究进展做一综述。     

支气管哮喘免疫学发病机理研究

本文主要介绍了近年对支气管哮喘发病机理的新认识，哮喘炎症形成的要理以及各种炎性细胞、炎性介质物别是细胞因子和粘附分子的作用，炎症与气道高反应性的关系。为进一步寻求防治哮喘新药提供理论基础。     

I—4在支气管哮喘发病中的作用研究

研究ＩＬ－４在促使支气管哮喘发病中的作用。方法用ＥＬＩＳＡ方法检测３９例支气管哮产妇和４６例正常产妇新生儿脐血中ＩＬ－４、ＩＦＮ－γ,ｓＩＬ－２Ｒ和总ＩｇＥ含量,并计数脐血嗜碱性粒细胞数,同时用免疫磁珠纯化脐血Ｔ淋巴细胞和嗜碱性粒细胞,以ＲＴ－ＰＣＲ和ＥＬＩＳＡ方法检测上述细胞刺激后表达ＩＬ－４ｍＲＮＡ和ＩＦＮ－γｍＲＮＡ以及其释放的蛋白质。结果哮喘脐血组ＩＬ－４、ＩＮＦ－γ和ｓＩＬ－２Ｒ含量和哮     

L-4在支气管哮喘发病中的作用研究

目的　研究IL 4在促使支气管哮喘发病中的作用。方法　用ELISA方法检测 39例支气管哮喘产妇和 46例正常产妇新生儿脐血中IL 4、IFN γ、sIL 2R和总IgE含量 ,并计数脐血嗜碱性粒细胞数 ,同时用免疫磁珠纯化脐血T淋巴细胞和嗜碱性粒细胞 ,以RT PCR和ELISA方法检测上述细胞刺激后表达IL 4mRNA和IFN γmRNA以及其释放的蛋白质。结果　哮喘脐血组IL 4、IFN γ和sIL 2R含量和嗜碱性粒细胞数虽高于正常脐血组 ,但两组差异无显著性 (P >0 .0 5 )。而纯化的脐血嗜碱性粒细胞表达IL 4mRNA和释放IL 4的水平 ,哮喘组显著高于正常脐血组 ,也高于T淋巴细胞。结论具有支气管哮喘遗传背景的新生儿脐血嗜碱性粒细胞对环境因素的刺激更加敏感 ,其释放的IL 4在促使支气管哮喘发病中起重要作用     

支气管哮喘与免疫

支气管哮喘已成为严重威胁人类健康的一种主要的疾病.全世界支气管哮喘患者有一亿人,我国的患者达一千万人,哮喘发病率为2.5%-9.0%,居于老年期疾病发病率的第二位.支气管哮喘患者多在夜间或凌晨发作,并有气短、胸闷和咳嗽等症状.因气道对多种刺激因子反应性增高,哮喘可反复发作,出现喘息和呼吸困难,甚至发生哮喘持续状态.     

支气管哮喘免疫学发病机理研究

本文主要介绍了近年对支气管哮喘发病机理的新认识，哮喘炎症形成的机理以及各种炎性细胞、炎性介质物别是细胞因子和粘附分子的作用，炎症与气道高反应性的关系。为进一步寻求防治哮喘新药提供理论基础。     

CD44和CD44v6基因在胃腺癌组织中的表达及其意义

目的： 研究胃腺癌组织中ＣＤ４４ 的表达及其与淋巴结转移和预后的关系。方法： 应用免疫组化方法, 对１０５ 例胃腺癌组织中ＣＤ４４ 的表达进行了观察, 并对其中６２ 例患者做了随访。结果： ＣＤ４４ 和ＣＤ４４ｖ６ 基因的表达率分别为５４－３ ％ 和４８－６ ％ 。ＣＤ４４ｖ６ 在胃腺癌组织中的表达与癌细胞的分化、浸润深度, 以及临床分期和预后有关（Ｐ＜ ０－０５）, 而ＣＤ４４ 的表达则与上述临床病理指标无关。另外, 抗ＣＤ４４ 和抗ＣＤ４４ｖ６ 抗体的阳性反应, 与癌细胞的淋巴结转移有关（ＣＤ４４ｖ６, Ｐ＜ ０－０１ ; ＣＤ４４ , Ｐ＜ ０－０５） 。结论： ＣＤ４４ 的表达可用于胃癌患者的病情监测, 其中ＣＤ４４ｖ６ 有望作为判断预后的一个指标。     

大鼠脊髓半横断损伤对脊髓versican、 CD44表达的影响

目的:观察大鼠脊髓半横断损伤(HSCI)对硫酸软骨素蛋白多糖versican及CD44在脊髓中表达的影响,为进一步探索脊髓损伤后的再生修复机制提供实验依据.方法:成年SD大鼠随机分为假手术对照组和HSCI 3、 7和14d组.建立成年大鼠HSCI(胸9～10)模型.取损伤位点头尾段胸9、胸10节段制作冷冻切片,用免疫组织化学ABC法和图像分析技术检测脊髓内versican和CD44的表达.结果:Versican阳性产物主要分布于细胞外基质中,腹角神经元和胶质细胞的胞质中也有分布;CD44阳性产物主要分布于细胞外基质及腹角神经元和胶质细胞的胞质中.损伤后脊髓内versican表达较对照组明显增加,CD44表达也较对照组明显增加.结论:HSCI后versican与CD44表达都增加,两者可能相互作用共同参与损伤后轴突生长被抑制这一过程.     

CD44与乳腺癌的研究进展

CD44基因蛋白是一种细胞表面跨膜糖蛋白,属于黏附分子家族。CD44基因外显子根据表达方式可分为标准型CD44(CD44s)和变异型CD44(CD44v)两种类型。大量研究发现,CD44蛋白参与细胞-细胞、细胞-胞外基质之间的特异性粘附,CD44及其亚型在乳腺癌的异常表达可能与癌症的发生、发展和转移密切相关。此外,乳腺癌干细胞表面高度表达CD44分子,针对CD44分子与乳腺癌干细胞关系的研究,将会为临床上对乳腺癌的诊断、治疗选择以及预后预测提供更充分的理论依据。     

CD44s在肺癌中的表达及其意义

目的:探讨CD44s在肺癌组织中的表达及其临床意义。方法:采用免疫组化方法检测117例原发性肺癌的CD44s异常表达。结果:CD44s在小细胞肺癌(SCLC)不表达,而表达于非小细胞肺癌(NSCLC)中,并且鳞癌的CD44s表达强度明显强于腺癌(P＜0.05);未发生淋巴结转移的肺癌病例中CD44s的表达明显高于已经发生转移的病例(P＜0.01);按照临床肿瘤TNM分期,早期肺癌的CD44s表达显著高于进展期肺癌(P＜0.05)。结论:CD44s对肺癌组织的病理类型、淋巴结转移、临床分期以及预后的判断是一个较好的评价指标。     

CD44 expression in soft tissue sarcomas

Recent studies have shown that expression of alternatively splicing variants of CD44 is correlated with prognosis for several kinds of malignant tumors. However, little is known about the expression of CD44 standard and variant isoforms in soft tissue sarcomas. In this study 47 cases of soft tissue sarcoma [18 malignant fibrous histiocytomas (MFHs), 13 synovial sarcomas (SSs), 7 malignant schwannomas (MSs), and 9 liposarcomas (LSs)] were examined immunohistochemically. The monoclonal antibodies to the standard form of CD44 (CD44H) and variant exons of CD44v3, 4, 5, 6, 7, 9, and v10 were used. We analyzed the membranous expression pattern of CD44H and CD44 variant exons and assessed the relation between expression of CD44s and metastasis-free survival rates (MFSR) of patients with soft tissue sarcoma. A few sarcomas expressed CD44v3 (2/47) and v7 (2/47), but none of the sarcomas expressed CD44v10. CD44v4 (5/47), v5 (4/47), v6 (10/47), and v9 (9/47) are relatively common types of variant isoforms in soft tissue sarcomas. Expression of CD44v6 is more frequently detected in high-grade than in low-grade tumors. CD44v6 or CD44v9 expression was correlated with metastasis-free survival of patients with soft tissue sarcomas. Received: 22 Juni 1999 / Accepted: 19 November 1999     

CD44+/CD24-细胞在乳腺癌组织及细胞系中的数量与分布

Objective To study the distribution and quantity of CD44VCD24- cells in breast cancer tissue and the cell lines,and as well as its correlation with the expression of various breast cancer markers and molecular subtyping of breast carcinoma.Methods The expression of CD44 / CD24,estrogen receptor,progesterone receptor,HER2,human estrogen-induced protein PS2,bcl-2 and nm23 in 60 cases of invasive ductal carcinoma of breast were studied by either single or double immunohistochemical staining.The co-expression of CD44 and CD24 in 3 breast cancer cell lines (MCF-7,MDA-MB-468,and MDA-MB- 231) was also examined.Results The quantity and distribution of CD44 + /CD24- cells varied greatly and no specific patterns were identified.The percentage of CD44 + /CD24- in breast cancer was 65%.The amount of CD44+/CD24- cells did not correlate with the age of patients,lymph node metastasis,tumor　 size,molecular subtypes and expression of various breast cancer markers in breast carcinoma.The proportion of CD44+/CD24- cells in MCF-7,MDA-MB-468,and MDA-MB-231 cell lines was < 1%,5% and > 80% ,respectively.Conclusions CD44+ /CD24- cells are demonstrated in certain breast cancer tissues and cell lines.However,there is no relationship obtained between the quantity or the distribution of these cells and the molecular subtyping or the clinicopathologic parameters in breast cancer.     

CD44+/CD24-细胞在乳腺癌组织及细胞系中的数量与分布

Objective To study the distribution and quantity of CD44VCD24- cells in breast cancer tissue and the cell lines,and as well as its correlation with the expression of various breast cancer markers and molecular subtyping of breast carcinoma.Methods The expression of CD44 / CD24,estrogen receptor,progesterone receptor,HER2,human estrogen-induced protein PS2,bcl-2 and nm23 in 60 cases of invasive ductal carcinoma of breast were studied by either single or double immunohistochemical staining.The co-expression of CD44 and CD24 in 3 breast cancer cell lines (MCF-7,MDA-MB-468,and MDA-MB- 231) was also examined.Results The quantity and distribution of CD44 + /CD24- cells varied greatly and no specific patterns were identified.The percentage of CD44 + /CD24- in breast cancer was 65%.The amount of CD44+/CD24- cells did not correlate with the age of patients,lymph node metastasis,tumor　 size,molecular subtypes and expression of various breast cancer markers in breast carcinoma.The proportion of CD44+/CD24- cells in MCF-7,MDA-MB-468,and MDA-MB-231 cell lines was < 1%,5% and > 80% ,respectively.Conclusions CD44+ /CD24- cells are demonstrated in certain breast cancer tissues and cell lines.However,there is no relationship obtained between the quantity or the distribution of these cells and the molecular subtyping or the clinicopathologic parameters in breast cancer.     

CD44+/CD24-细胞在乳腺癌组织及细胞系中的数量与分布

Objective To study the distribution and quantity of CD44VCD24- cells in breast cancer tissue and the cell lines,and as well as its correlation with the expression of various breast cancer markers and molecular subtyping of breast carcinoma.Methods The expression of CD44 / CD24,estrogen receptor,progesterone receptor,HER2,human estrogen-induced protein PS2,bcl-2 and nm23 in 60 cases of invasive ductal carcinoma of breast were studied by either single or double immunohistochemical staining.The co-expression of CD44 and CD24 in 3 breast cancer cell lines (MCF-7,MDA-MB-468,and MDA-MB- 231) was also examined.Results The quantity and distribution of CD44 + /CD24- cells varied greatly and no specific patterns were identified.The percentage of CD44 + /CD24- in breast cancer was 65%.The amount of CD44+/CD24- cells did not correlate with the age of patients,lymph node metastasis,tumor　 size,molecular subtypes and expression of various breast cancer markers in breast carcinoma.The proportion of CD44+/CD24- cells in MCF-7,MDA-MB-468,and MDA-MB-231 cell lines was < 1%,5% and > 80% ,respectively.Conclusions CD44+ /CD24- cells are demonstrated in certain breast cancer tissues and cell lines.However,there is no relationship obtained between the quantity or the distribution of these cells and the molecular subtyping or the clinicopathologic parameters in breast cancer.     

CD44+/CD24-细胞在乳腺癌组织及细胞系中的数量与分布

Objective To study the distribution and quantity of CD44VCD24- cells in breast cancer tissue and the cell lines,and as well as its correlation with the expression of various breast cancer markers and molecular subtyping of breast carcinoma.Methods The expression of CD44 / CD24,estrogen receptor,progesterone receptor,HER2,human estrogen-induced protein PS2,bcl-2 and nm23 in 60 cases of invasive ductal carcinoma of breast were studied by either single or double immunohistochemical staining.The co-expression of CD44 and CD24 in 3 breast cancer cell lines (MCF-7,MDA-MB-468,and MDA-MB- 231) was also examined.Results The quantity and distribution of CD44 + /CD24- cells varied greatly and no specific patterns were identified.The percentage of CD44 + /CD24- in breast cancer was 65%.The amount of CD44+/CD24- cells did not correlate with the age of patients,lymph node metastasis,tumor　 size,molecular subtypes and expression of various breast cancer markers in breast carcinoma.The proportion of CD44+/CD24- cells in MCF-7,MDA-MB-468,and MDA-MB-231 cell lines was < 1%,5% and > 80% ,respectively.Conclusions CD44+ /CD24- cells are demonstrated in certain breast cancer tissues and cell lines.However,there is no relationship obtained between the quantity or the distribution of these cells and the molecular subtyping or the clinicopathologic parameters in breast cancer.     

CD44+/CD24-细胞在乳腺癌组织及细胞系中的数量与分布

Objective To study the distribution and quantity of CD44VCD24- cells in breast cancer tissue and the cell lines,and as well as its correlation with the expression of various breast cancer markers and molecular subtyping of breast carcinoma.Methods The expression of CD44 / CD24,estrogen receptor,progesterone receptor,HER2,human estrogen-induced protein PS2,bcl-2 and nm23 in 60 cases of invasive ductal carcinoma of breast were studied by either single or double immunohistochemical staining.The co-expression of CD44 and CD24 in 3 breast cancer cell lines (MCF-7,MDA-MB-468,and MDA-MB- 231) was also examined.Results The quantity and distribution of CD44 + /CD24- cells varied greatly and no specific patterns were identified.The percentage of CD44 + /CD24- in breast cancer was 65%.The amount of CD44+/CD24- cells did not correlate with the age of patients,lymph node metastasis,tumor　 size,molecular subtypes and expression of various breast cancer markers in breast carcinoma.The proportion of CD44+/CD24- cells in MCF-7,MDA-MB-468,and MDA-MB-231 cell lines was < 1%,5% and > 80% ,respectively.Conclusions CD44+ /CD24- cells are demonstrated in certain breast cancer tissues and cell lines.However,there is no relationship obtained between the quantity or the distribution of these cells and the molecular subtyping or the clinicopathologic parameters in breast cancer.