高效液相色谱法测定血浆中布洛芬浓度

建立HPLC法测定健康人血浆中布洛芬浓度的方法。用反相C_(18)柱,甲醇-乙腈-0.02mol/L醋酸钠一醋酸溶液(47:23:30)V/V为流动相,检测波长225nm。结果布洛芬的线性范围为0.11～53.83μg/ml,γ=0.9999,最低检出浓度0.1μg/ml,平均回收率为100.6％,日间变异系数(RSD％)<7％。本法操作简单,快速,准确。     

高效液相色谱法测定血浆中布洛芬浓度

建立HPLC法测定健康人血浆中布洛芬浓度的方法。用反相C₁₈柱,甲醇-乙腈-0.02mol/L醋酸钠一醋酸溶液(47:23:30)V/V为流动相,检测波长225nm。结果布洛芬的线性范围为0.11～53.83μg/ml,γ=0.9999,最低检出浓度0.1μg/ml,平均回收率为100.6％,日间变异系数(RSD％)<7％。本法操作简单,快速,准确。     

复方布洛芬软胶囊中2组分溶出度的测定方法研究

目的 :建立同时测定复方布洛芬软胶囊中布洛芬和对乙酰氨基酚2组分溶出度的方法。方法 :以磷酸盐缓冲液 (pH=7 2)为溶剂 ,转速为75r/min ,取样时间为45min ,采用反相高效液相色谱法测定布洛芬和对乙酰氨基酚的溶出度 ,其中色谱柱为氰基柱 ,流动相为磷酸盐缓冲液 (pH=6 6) -甲醇 (60∶40) ,流速为1 0ml/min ,检测波长为223nm ,柱温为30℃。结果 :对乙酰氨基酚与布洛芬检测浓度线性范围分别为0 17～100 14μg/ml(r=0 9999 ,n=9)、0 21～124 86μg/ml(r=0 9999 ,n=9) ;平均回收率分别为99 62 %(RSD=0 36 %)、99 79 %(RSD=0 49 %)。结论 :本方法简便、快速、准确、可靠 ,能同时测定复方布洛芬软胶囊中2组分的溶出量。     

高效液相色谱法测定退烧颗粒中黄芩苷的含量

目的 用高效液相色谱法测定退烧颗粒中黄芩苷的含量。方法 色谱条件为AllfimaC18柱(250mm×4.6mm),流动相:甲醇-2％醋酸(47:53),检测波长:270nm,流速:0.8ml/min,柱温30℃。结果 黄芩苷在0.08-0.4ug(r=0.9994)范围线性关系良好。加样回收率为96.80％(RSD=1.17％)。结论 该实验方法简便,可控。     

HPLC法测定头孢呋辛酯及其片剂的含量

建立头孢呋辛酯及其片剂的含量测定方法。以岛津Shim-Pack C_(18)(4.6 mm×150 mm,5μ)色谱柱,以0.2 mol/L磷酸二氢铵溶液-甲醇(62:38)为流动相,以278 nm为检测波长。线性范围为0.05～2 mg/ml,回归方程为Y=2.69×10~6X-1.87×10~5,r=0.9999,平均回收率为99.4％(RSD=0.9％,n=9)。     

高效液相色谱法测定布洛芬片的含量

目的:建立高效液相色谱法测定布洛芬片含量.方法:使用Waters SymmetryC18色谱柱,流动相为0,1mol/L磷酸二氢钾溶液-乙腈(37:63),检测波长为263nm,流速1.0ml/min.线性范围为0.040～0.640mg/ml,r=0.9998,平均回收率为99.87%.结果:布洛芬分离好.结论:本方法可以快速准确检测布洛芬片含量.     

高效液相色谱法测定血浆中酮基布洛芬的含量

建立测定血浆中酮基布洛芬简便、灵敏的HPLC法.色谱柱为SpherisorbC18柱(10cm×4.6mm,3μ),流动相为5mmol/L磷酸盐缓冲液(pH6.8)-甲醇(28∶72,V/V),流速1ml/min,在UV262nm波长处检测,检测器灵敏度设为0.005aufs,响应时间0.2s.采用内标法定量,内标物为萘普生.1ml血浆样品盐酸酸化后加入6ml乙醚提取,提取液空气吹干,用250μl流动相重溶解后进行分析.实验结果表明,在此分析条件下酮基布洛芬和内标分离完全且无其它干扰,保留时间分别为12.3和9.5min.在0.01～5.0μg/ml浓度范围内酮基布洛芬峰面积比与其浓度间呈良好的线性关系,r=0.9999,血浆中酮基布洛芬最低检测浓度为3ng/ml,回收率85.9%～90.9%,天内变异1.4%～4.9%,天间变异2.5%～6.2%.     

布洛伪麻分散片的溶出度测定

建立了布洛伪麻分散片的溶出度检查方法.考察在不同的溶出介质、转速下布洛伪麻分散片中布洛芬和盐酸伪麻黄碱的溶出情况,确定了溶出参数:照中国药典2010年版溶出度第二法装置,以pH 5.5的磷酸盐缓冲液900ml为溶出介质,转速50 r/min.采用HPLC法测定每片中布洛芬和盐酸伪麻黄碱的溶出量.使用C18色谱柱,流动相为乙腈-水(1∶1)(每1 000ml含十二烷基磺酸钠2.5 g,加磷酸1 ml,混匀后用氨水调至pH 3.2),检测波长215 nm.布洛芬和盐酸伪麻黄碱在10～1 000 μg/ml和1.5～150 μ.g/ml范围内线性关系良好,回收率为98.1％和100.4％,RSD为2.6％和0.4％.     

HPLC法测定头孢呋辛酯及其片剂的含量

建立头孢呋辛酯及其片剂的含量测定方法。以岛津Shim-Pack C₁₈(4.6 mm×150 mm,5μ)色谱柱,以0.2 mol/L磷酸二氢铵溶液-甲醇(62:38)为流动相,以278 nm为检测波长。线性范围为0.05～2 mg/ml,回归方程为Y=2.69×10⁶X-1.87×10⁵,r=0.9999,平均回收率为99.4％(RSD=0.9％,n=9)。     

布洛芬愈创木酚酯及有关物质的HPLC测定

建立了HPLC法测定布洛芬愈创木酚酯的含量及有关物质.采用Spherisorb C18色谱柱,流动相为乙腈-水-乙酸(79:20:1),流速1ml/min,检测波长223nm.布洛芬愈创木酚酯在2.5～200μg/ml范围内线性关系良好(r=0.9998),平均回收率99.1%,检测限为5ng.     

Comparison of the antinociception produced by two oral formulations of ibuprofen: ibuprofen effervescent vs ibuprofen tablets

Objective: The aim of this study was to compare the dose-related effects of both ibuprofen tablets and ibuprofen effervescent [placebo, 400 and 800 mg ibuprofen (Aktren)] on phasic pain. Patients: Twenty volunteers participated in this randomized, double-dummy, fivefold crossover study. Methods: Measurements were obtained before and 15, 60 and 240 min after drug administration. Pain was produced by CO₂ pulses applied to the left nostril. Subjects rated the intensity of the painful stimuli by means of a visual analogue scale. In addition, chemosomatosensory event-related potentials were recorded. Results: In line with previous work, ibuprofen produced a dose-related decrease in pain-related potential amplitudes P1N1, indicating its antinociceptive effects. Higher plasma concentrations of ibuprofen were reached 15–40 min after administration of the effervescent while ibuprofen tablets had a t_max 60–90 min after administration. In addition, 60 min after intake of the effervescent a prolongation of the latencies of the potentials was observed, possibly reflecting superior antinociceptive properties when compared to ibuprofen tablets. In addition, the effervescent appeared to have more consistent effects on intensity estimates compared to ibuprofen tablets. Received: 13 June 1996 / Accepted in revised form: 22 November 1996     

Tissue levels of ibuprofen after fatal overdosage of ibuprofen and acetaminophen

Ibuprofen is a non-steroidal anti-inflammatory agent which is relatively safe and effective for the treatment of inflammatory disorders, but there are several reports of acute poisoning due to ibuprofen overdosage. Although the majority of ibuprofen overdosages are not very serious, acute renal failure, hepatic failure, and hypotension and coma have been described. We report here the case of a 48-year-old male who committed suicide by ingesting overdosages of both ibuprofen and acetaminophen. The case is unique because of the role ibuprofen played in his death, and because we were able to measure levels of ibuprofen in tissues obtained from post mortem examination. Tissue levels of ibuprofen have not previously been reported in humans.     

精氨酸布洛芬糖浆与布洛芬片剂人体药动学及生物等效性比较

目的:比较精氨酸布洛芬糖浆与布洛芬片在健康人体中的药动学及生物等效性。方法:采用液相色谱法测定18位健康男性受试者交叉服用精氨酸布洛芬糖浆和布洛芬片后的血浆中布洛芬浓度。结果:糖浆剂的药动学参数为:AUC_(0-10)=(150±s 17)mg·h·L~(-1),C_(max)=(47±9)mg·L~(-1),t_(max)=(0．6±0．3)h;布洛芬片剂的药动学参数为:AUC_(0-10)=(136±13)mg·L~(-1),c_(max)=(28±4)mg·L~(-1),t_(max)=(2．7±1．0)h,相对生物利用度为 (98±7)％。结论:精氨酸布洛芬糖浆和布洛芬片生物等效。     

RP-HPLC法同时测定血浆中布洛芬丁香酚酯和布洛芬的含量

目的建立测定血浆中布洛芬丁香酚酯及布洛芬含量的HPLC方法,并应用于药物动力学研究。方法采用RP-HPLC法。色谱柱:ODS色谱柱;流动相:乙腈-甲醇-体积分数为0.2%的三氟乙酸-四氢呋喃(体积比为50∶10∶15∶2);流速:1.0 mL.min-1;检测波长:230 nm;室温测定。布洛芬、内标甘草次酸、布洛芬丁香酚酯的保留时间分别为5.62、15.981、8.05 min。结果布洛芬和布洛芬丁香酚酯的的线性范围分别为0.64~64μg.L-1和0.8~80μg.L-1;最低检测限分别为0.64 mg.L-1和0.16 mg.L-1,日内和日间变异系数小于10%,精密度大于90%。结论该方法灵敏、快速、准确,操作简便、线性范围宽,可适用于同时测定血浆中布洛芬丁香酚酯和布洛芬的含量。