维拉帕米对人血PMN细胞呼吸暴发和氧自由基的作用

用化学发光法观察了维拉帕米对人血ＰＭＮ细胞受ＰＭＡ刺激发生呼吸暴发生的活性氧，黄嘌呤－黄嘌呤－黄呤氧化酶体系产生的Ｏ＾－２，ＶｉｔＣ－Ｃｕ＾２＋－母多糖体系产生的．ＯＨ，及Ｈ２Ｏ２的释放反应的影响。     

戈米辛J对氧自由基的作用

目的 研究戈米辛Ｊ（ｇｏｍｉｓｉｎ Ｊ）对氧自由基的作用。方法 采用黄嘌呤／黄嘌呤氧化酶－鲁米诺体系和邻苯三酚自氧人体系产生的化学发光检测超氧阴离子自由基,采用Ｆｅｎｔｏｎ发光体系检测羟自由基。结果 戈米辛Ｊ能抑制３的化学发光,对以上３种体系的ＩＣ５０、ＩＣ２０及ＩＣ５０（９５％可信限）依次分别为２３７．７（１５５．５～３６３．３）、１００．８（８８．１～１１５．３）和１．８６（１．６０～２．１６     

PMN对链球菌组蛋白样蛋白释放的影响

为观察人多形核白细胞 (PMN)对链球菌组蛋白样蛋白 (HlpA)释放的影响 ,将PMN与链球菌以一定比例共同作用后取上清液做免疫印迹试验。发现与抗HlpA抗体特异性结合的蛋白条带。提示PMN作用于链球菌后 ,HlpA能自菌体内释放出来     

大鼠脊髓压迫伤后PMN浸润的变化及MPS的治疗作用

脊髓损伤在战时和平时的创伤发生率中均占有一定的比例 ,其原发损伤后继发性损伤是致瘫的重要原因 ,但确切机制目前尚不清楚。近年来多形核白细胞 (ＰＭＮ)在继发性脊髓损伤中的作用受到重视[1] ,研究ＰＭＮ在脊髓继发性损伤中的作用及其机制具有重要意义。1　材料与方法1.1　动物的压迫损伤模型　　选用 77只Ｗｉｓｔａｒ大鼠 ,体重 2 0 0～ 30 0ｇ ,腹腔麻醉后 ,俯卧位固定 ,于Ｌ1水平行椎板切除术 ,暴露脊髓。动物稳定 1ｈ后 ,用 30ｇ重物压Ｌ1节段脊髓 2 0ｍｉｎ。1.2　实验分组1.2 .1　分组　　实验共分 3组 :伤前组、损伤组和甲基强的…     

多形核白细胞对血管内皮细胞的毒性作用

本文利用体外培养血管内皮细胞的方法,观察了酵母多糖活化的多形核白细胞(PMN)对内皮细胞的影响。本研究发现:将活化的PMN与内皮细胞孵育,10h内皮细胞出现核固缩等变化,培养液中LDH升高;24h内皮细胞变化更为明显,细胞变性坏死,培养液中LDH和6-酮-PGF_1α含量均明显升高。予先向培养液中分别加入超氧化物歧化酶(SOD)或过氧化氢酶(CAT),内皮细胞仍有损害,将SOD和CAT予先联合加入,可保护内皮细胞免受PMN的损害。据此认为:活化PMN引起的内皮细胞损伤可能主要系羟自由基(OH·)所致。     

利用微量全血化学发光检测白细胞吞噬功能

应用化学发光分析方法轩分离的多形核白细胞（ＰＭＮ）和全血中ＰＭＮ的吞噬活性进行了测定，并对影响全血化学发光分析方法的主要因素进行了实验观察。结果发现最佳实验条件为：鲁米诺浓度１０＾－１ｍｏｌ／Ｌ，血液浓度２％，反应温度３７℃，调理酵母多糖浓度１０ｇ／Ｌ。同时全血测定法具有以下优点；（１）用血量极少；（２）无需进行白细胞分离，更接近体内环境；９３）结果较分离方法更好。     

急性肺损伤大鼠肺组织PMN扣押的变化及山莨菪碱的影响

多形核白细胞（Ｐｏｌｙｍｏｒｐｈｏｎｕｃｌｅａｒ　ｌｅｕｋｏｃｙｔｅ，ＰＭＮ）于肺内大量扣押是急性肺损伤（Ａｃｕｔｅ ｌｕｎｇ ｉｎｊｕｒｙ，ＡＬＩ）和急性呼吸窘迫综合征（Ａｃｕｔｅ ｒｅｓｐｉｒａｔｏｒｙ ｄｉｓｔｒｅｓｓ　ｓｙｎｄｒｏｍｅ，ＡＲＤＳ）发病过程中的早发事件，是ＰＭＮ介导肺血管内皮细胞、肺泡上皮细胞广泛损伤致通透性增加、肺水肿及微血栓形成的关键所在［１］。髓过氧化物酶（Ｍｙｅｌｏｐｅｒｏｘｉｄａｓｅ，ＭＰＯ）主要存在于ＰＭＮ中，每个ＰＭＮ所含的酶量都是一定的，因此组织ＭＰＯ活力可能反…     

Inhibiting effects of verapamil on respiratory burst of polymorphonuclear leucocytes

ＩｎｈｉｂｉｔｉｎｇｅｆｆｅｃｔｓｏｆｖｅｒａｐａｍｉｌｏｎｒｅｓｐｉｒａｔｏｒｙｂｕｒｓｔｏｆｐｏｌｙｍｏｒｐｈｏｎｕｃｌｅａｒｌｅｕｃｏｃｙｔｅｓＬｉｕＸｕｅｆｅｎ（刘雪芬）；ＣｈｅｎＳｈｕｙｕａｎ（陈树元）；ＬｉａｏＸｉｌｉｎｇ（廖锡麟）（Ｄｅ...     

Effects of Schisanhenol on respiratory burst in rat neutrophils

Schisanhenol(Sal),anactivecomponentisolatedfrailSchizandraerubriflora,hasbeendemonstratedtohaveprotectiveeffectagainstCCI',galactosamineandacetaminopheninducedliverinjuryandsuggestedasananti--oxid..t(1'2).Histologicalinvestigationsshowedthattherewereinfiltrationofpolymorphonuclearcellsandmacrophagesintheinjuredliverinducedbygalactosamine(').Schlay,etal(4)discoveredthatbothneutrophildegenerationandlivercellleakageinthegalactosamineinducedliverinjurycanbeprotectedbyturpentinepretreatment,sugge…     

Protective effects of verapamil on myocardial mitochondrial respiratory function after combined radiation－burn injury in rats

Ｐｒｏｔｅｃｔｉｖｅｅｆｆｅｃｔｓｏｆｖｅｒａｐａｍｉｌｏｎｍｙｏｃａｒｄｉａｌｍｉｔｏｃｈｏｎｄｒｉａｌｒｅｓｐｉｒａｔｏｒｙｆｕｎｃｔｉｏｎａｆｔｅｒ　ｃｏｍｂｉｎｅｄｒａｄｉａｔｉｏｎ－ｂｕｒｎｉｎｊｕｒｙｉｎｒａｔｓＸｉｏｎｇＹｅ（熊业）；Ｃ...     

维拉帕米对创伤后大鼠痛觉过敏的影响

目的 观察维拉帕米对创伤后痛觉过敏的影响。方法 Ｗｅｓｔａｒ大鼠４８只，以截肢作为创伤模型。大鼠创伤后分别腹腔注射不同剂量的维拉帕米、芬太尼、芬太尼＋维拉帕米以及生理盐水，于创伤后１５、３０、６０、１２０ｍｉｎ观察创伤部位周围皮肤痛阈变化情况。结果 １０ｍｇ／ｋｇ维拉帕米即可抑制创伤部位周围皮肤的痛阈下降，其中芬太尼＋维拉帕米作用最强，结论 维拉帕米可抑制痛常见过敏，具有一定的镇痛作用并加强芬太尼     

维拉帕米对放烧复合伤大鼠心肌线粒体功能的保护作用

用钙通道阻滞剂维拉帕米治疗和预防放烧复合伤（６Ｇｙ放射损伤合并３０％Ⅲ度烧伤）大鼠，观察其血压和心肌线粒体呼吸功能的变化及的作用。预防或治疗给药对伤后动脉血压有一定影响，显著保护伤后心肌线粒体呼吸功能。维拉帕米的保护作用可能与它减少了心肌线粒体钙浓度有关。     

葛根素注射液对家兔肝缺血再灌注损伤的防治作用

目的:观察葛根素注射液对家兔肝缺血再灌注损伤(HIRI)的防治作用.方法:制备家免HIRI模型,30只健康家免随机分为3组:假手术组(S组)10只,缺血再灌注组(IR组)10只,葛根素组(Pur组)10只.观察肝组织及血中超氧化物歧化酶(SOD)活性、黄嘌呤氧化酶(XO)活性、谷胱甘肽过氧化物酶(GSH-Px)活性、谷丙转氨酶(ALT)活性、丙二醛(MDA)含量及肝细胞形态学变化.结果:肝缺血再灌注后,葛根素组与缺血再灌注组比较,血浆中SOD、XO、MDA、GSH-Px、ALT差异均有显著性或非常显著性(P＜0.05或P＜0.01);肝细胞形态学异常改变明显减轻.结论:葛根素注射液通过拮抗脂质过氧化反应而对HIRI具有良好的防治作用.     

维拉帕米对光动力疗法中SW480癌细胞株内游离钙与光敏剂的影响

目的：探讨维拉帕米在光动力学治疗结肠癌中增效作用及机制。方法：在结肠癌细胞培养的基础上，采用荧光分光光度法、ＭＴＴ法、荧光探针法等测定维拉帕米增效后癌细胞内游离钙、癌细胞存活率和细胞内光敏剂含量的变化。结果：维拉帕米可显著增加癌细胞内光敏剂含量和光动力学疗法（ＰＤＴ）杀伤作用，激光照射后癌细胞存活率与细胞内光敏剂含量呈显著负相关。癌细胞内光敏剂在８～１６ｈ即可达到较高浓度。加维拉帕米后，癌细胞内游离钙降低，激光照射后游离钙明显增加，与癌细胞损伤程度一致。结论：维拉帕米在ＰＤＴ治疗大肠癌中具有明显的增效作用，胞内游离钙起重要作用。     

Effect of zinc-treated Entamoeba histolytica on the human polymorphonuclear respiratory burst

One of the mechanisms that Entamoeba histolytica uses to evade host immune response is inhibition of the polymorphonuclear (PMN) leukocyte respiratory burst. In studies previously conducted in a model used in our laboratory, we observed that when treating trophozoites with different zinc concentrations certain amebic functions are inhibited while significantly limiting development of hepatic abscess in golden hamsters (Mesocricetus aureatus). We carried out an in vitro study using a chemoluminescent method to assess the effect zinc-treated amebic trophozoites exercise on respiratory burst in human PMNs. We measured response of PMNs incubated with E. histolytica trophozoites from cultures with TYI-S33 medium alone and with zinc. Zinc concentrations between 0.1 and 1.0 mM did not affect amebic trophozoite viability, and PMNs in contact with these in a zinc-free medium had an oxidative response similar to that obtained with zymosan and significantly greater (p <0.05) than that generated by cells co-incubated with amebas cultured in TYI-S33 medium alone. These results suggest that zinc alters the amebic mechanism that inhibits the oxidative function of human polymorphonuclear leukocyte.     

Experimental Study on the Inhibitory Effects of Verapamil on the Proliferation of Meningiomas Cells

In order to investigate the effects of verapamil on the proliferation of meningiomas cells in vitro and in vivo, the cultured meningiomas cells were cultured with verapamil at different concen-trations for 24 h and the inhibitory effects of verapamil on cell proliferation were observed by MTT method. The meningiomas model was established by implanting the newly removed tumor fragments into the nude mice subcutaneously. The nude mice with tumors were divided into two groups: vera-pamil-treated group and control group. Tumor volumes were measured and after 12 weeks the tumors were taken out and examined histologically. The expression of proliferating cell nuclear antigen (PCNA) in the tumors was detected by using immunohistochemistry. It was found that verapamil could inhibit the growth of cultured meningiomas cells in a concentration-dependant manner. The in-hibitory effect could be observed in the concentration of 1 μmol/L verapamil and the most obvious effects appeared in the concentration of 100 μmol/L. Tumor volume in the verapamiltreated group was obviously smaller than that in the control group (211.40±5.50 vs 163.94±3.62, P<0.01) and the expression of PCNA was also lower (1.52±0.24 vs 2.86±0.53, P<0.05). Tumor inhibition rate was about 22.45%. It was suggested that verapamil could inhibit the proliferation and growth of men-ingiomas cells in vitro and in vivo.     

维拉帕米对博莱霉素A5所致大鼠肺损伤的防治作用

目的　探讨钙拮抗剂维拉帕米 (verapamil)对博莱霉素所致大鼠肺损伤的防治作用。方法　将 4 8只 SD大鼠一次性气管内注入博莱霉素 A5(5 mg· kg- 1 )制备肺损伤模型 ,次日随机分为模型对照组和实验组 ,d2开始 ,模型组给予生理盐水 ,实验组给予维拉帕米灌胃给药。测定分析各组给药后 d7、d14、d2 8肺组织羟脯氨酸 (HP)、脂质过氧化物 (L PO)含量以及组织病理学变化 ,并与空白对照组 (n=15 )比较。结果　实验组各期肺组织 L PO及 HP含量均较模型对照组明显降低 (P<0 .0 5 ,P<0 .0 1) ,组织病理学显示肺泡炎及肺纤维化程度均明显减轻。结论　钙拮抗剂维拉帕米对博莱霉素所致大鼠肺损伤具有保护作用。