白血病患者外周血T淋巴细胞亚群和SOD水平

据文献报告[1] ,超氧化物歧化酶与肿瘤的关系颇受临床的关注。而有关白血病患者ＳＯＤ水平与Ｔ淋巴细胞亚群水平的报道不多 ,为此我们进行了探讨 ,旨在了解白血病患者的免疫功能的变化 ,现报道如下。对象和方法一、对象 :(一 )正常人 :35人。均为我院预防保健科体检合格的健康人 ,无心、肝、肺、肾等重要脏器疾患 ,肝、肾功能试验正常 ,无血液病史。(二 )病人组 :32人。均为临床、血常规、骨髓像、常规组织化学染色检查确诊。其中急性非淋巴细胞性白血病 12例 ,Ｍ14例、Ｍ2 6例、Ｍ52例 ,急性淋巴细胞白血病 2 0例 ,Ｌ16例、Ｌ2 9例、Ｌ35例…     

带状疱疹患者外周血T淋巴细胞亚群分析

带状疱疹患者外周血Ｔ淋巴细胞亚群分析李大宁，余红上海第二医科大学附属新华医院皮肤科上海２０００９２带状疤疹是一种由水痘一带状疱疹病毒（ＶＺＶ）感染引起的疾病，机体对ＶＺＶ的特异性免疫目前认为主要是细胞免疫。本文采用流式细胞仪（ＦＣＭ）技术测定带状疤疹...     

丙型肝炎患者外周血T淋巴细胞亚群的变化

丙型肝炎患者外周血Ｔ淋巴细胞亚群的变化李灼亮，郑茉莉，谢庆，涂荫国，黄振国空军广州医院传染病科广州５１０６０２一般认为［１～４］慢性乙型肝炎患者外周血Ｔ淋巴细胞亚群的变化，可反映患者全身免疫状态，ＣＤ８＋细胞显著升高，ＣＤ４＋／ＣＤ８＋比值下降，可能...     

肾小球疾病患者外周血T淋巴细胞亚群的观察

细胞免疫在肾小球肾炎的发生发展过程中发挥着重要的作用[1] ,但慢性肾小球肾炎患者外周血Ｔ淋巴细胞亚群在临床上尚无一致的结论[2 ] ,可能与运用不同的检测方法有一定关系。本文运用流式细胞仪 ,检测肾病患者外周血Ｔ淋巴细胞亚群 ,藉以探讨对二者之间的关系。1　临床资料共检 15 4例 ,年龄 14～ 78岁 ,中位数 38岁。慢性肾炎41例 ,肾病综合征 44例 ,慢性肾衰 (原发性肾小球 ) 5 4例 ,狼疮性肾炎 (ＬＮ ) 7例 ,紫癜性肾炎 8例。另外 ,根据血浆白蛋白情况将肾病综合征分为白蛋白 >2 5ｇ/Ｌ组 (Ａ组 )和白蛋白 <2 5ｇ/Ｌ组 (Ｂ组 ) ,Ａ组血…     

外周血T淋巴细胞亚群及细胞因子水平检测在胃癌诊断中的意义

目的:探讨外周血T淋巴细胞亚群及白介素(IL)2、6、12水平检测在胃癌诊断中的意义。方法:检测胃癌患者血清中CD3、CD4、CD8、IL-2、IL-6、IL-12水平,并与正常对照组比较。结果:胃癌患者血清中CD3、CD4、IL-2、IL-12水平显著低于对照组,CD8、IL-6则显著高于对照组。结论:检测外周血CD3、CD4、CD8、IL-2、IL-6、IL-12水平对胃癌诊断具有重要价值。     

妊高征患者血清TNF-α和外周血B细胞及T淋巴细胞亚群水平检测的临床意义

目的：探讨妊高征患者血清TNF-α和外周血B和T淋巴细胞亚群水平及临床意义。方法：分别应用放免法和单克隆抗体法对34例妊高征患者进行了血清，TNF-α和外周血B及T淋巴细胞亚群水平检测，并与35名正常健康人作比较。结果：妊高征患者血清TNF-α、B细胞数量均显著高于正常对照组（P〈0．01），而CD3、CD4、CD4／CD8比值则显著低于正常对照组（P〈0．01）。结论：妊高征是一种和自身免疫调节异常相关的疾病。     

肺癌患者外周血T淋巴细胞及亚群CD28表达和活化的改变

目的检测共刺激分子CD28在肺癌患者外周血T淋巴细胞及亚群中的表达密度和细胞活化程度的变化,并探讨该变化与肺癌临床分期的关系.方法用三色荧光直标流式细胞法分别检测38例正常人与42例肺癌患者中血T淋巴细胞亚群中CD28的阳性率以及CD28+T淋巴细胞亚群活化率.结果与正常人相比,肺癌患者外周血中的CD3+CD28+,CD4+CD28+,CD8+CD28+淋巴细胞百分比均有下降(P＜0.05).淋巴细胞及其T亚群中的CD25+CD28+,CD38+CD28+淋巴细胞百分比亦有所下降(P＜0.05),但在不同临床分期的患者之间无显著性差异(P＞0.05).结论肺癌患者外周血T淋巴细胞的CD28表达密度及CD28+T细胞的活性均有降低,但下降幅度与临床分期无关.     

角膜移植排斥反应前后患者外周血T淋巴细胞亚群及NK细胞检测

角膜移植排斥反应前后患者外周血Ｔ淋巴细胞亚群及ＮＫ细胞检测马传香，纪恩美，郭宝强，王继兵，许福亮潍坊医学院附属医院中心实验室潍坊２６１０３１在角膜移植手术的患者中，部分病例难以避免角膜移植排斥反应的发生。山口达夫曾报告：初次角膜移植手术排斥反应的发生...     

冠心病患者治疗前后血清SOD、ET及T淋巴细胞亚群水平

目的:探讨了冠心病患者治疗前后血清SOD、ET及T淋巴细胞亚群水平。方法:分别应用放免法和单克隆抗体法对42例冠心病患者进行了血清SOD、ET及T淋巴细胞亚群水平检测,并与35名正常健康人作比较。结果:冠心病患者在治疗前血清ET水平显著地高于正常人组(P<0.01),而SOD和CD4/CD8比值明显地低于正常人组(P<0.01),经治疗后一个月则与正常人组比较差异无显著性(P>0.05)。结论:检测冠心病患者血清SOD、ET及T淋巴细胞亚群水平对判断病情及其预后均具有一定的临床实用价值。     

Activity patterns assessed throughout 24-hour recordings in preterm and near term infants

The motility of 10 low-risk infants, aged between 34 and 40 weeks of postmenstrual age, has been continuously recorded for 24 h. Four codes were distinguished: code 1 (absence of motility or occasional occurrence of startles), code 2 (presence of small general or isolated body movements, startles, smiles, grimaces, and other facial activity), code 3 (forceful and prolonged general movements, startles, and stretches), code 4 (vigorous and abrupt general body movements accompanied by crying). Changes with age concern mainly the increase of the duration of code 1 (quiescence) episodes. Confrontation between day and night showed higher levels of motility during the night than during the day. The last weeks before term represent a time for increase in the ability to sustain a quiet behavior and to reorganize day-night motility distribution.     

Evaluation of lymphocyte phenotype and phytohemagglutinin response in healthy very low birth weight infants

Sixteen healthy very low birth weight infants (VLBWI) were studied in a serial fashion over a 3-week period. Subjects were evaluated for lymphocyte phenotype, phytohemagglutinin (PHA) response, and metabolic status including weight, blood urea nitrogen, creatinine, albumin, pH, calcium, phosphorus, and ammonia. Lymphocyte phenotype determination showed a decreased proportion of CD3+ cells (66.8 +/- 10.4 vs 75.9 +/- 6.1, P less than 0.02) in VLBWI. When subsets of the CD4+ population were examined, VLBWI had a lower proportion of CD4+/CD29+ cells (8.2 +/- 5.8% vs 23.5 +/- 8.0%, P less than 0.0001) than adults and a higher proportion of CD4+/CD45R+ cells (35.6 +/- 12.4% vs 22.2 +/- 7.4%, P less than 0.03). The CD4+ subsets in VLBWI were similar to those seen in term infants. The peak PHA response in VLBWI was greater than that of adults (P less than 0.01). There was little change in the immune measurements over the 3-week study period. There were no strong correlations between any of the immunological measurements and the metabolic measurements except that the proportion of CD8+ cells increased with birth weight. Our findings demonstrate that immune measurements in healthy VLBWI differ from values found in adults but are similar to those of full-term infants. Lower proportions of the CD4+/CD29+ cells (the helper/inducer subset for antibody production) may contribute to some of the differences in immune function reported in neonates.     

Intraocular pressure in very low birth weight preterm infants and its association with postconceptional age

OBJECTIVE:

To evaluate intraocular pressure in very low birth weight preterm infants and correlate it with postconceptional age.

METHODS:

The intraocular pressure in a prospective cohort of very low birth weight premature infants (defined as a birth weight ≤1,500 g and gestational age ≤32 weeks) admitted to Hospital de Clínicas de Porto Alegre, Brazil was evaluated weekly. The evaluated outcome was the variation in the intraocular pressure following changes in the postconceptional age (defined as the gestational age at birth plus the age in weeks at the time of examination) in the weeks following preterm birth. Mixed-effects models were used for the statistical analysis to determine the intraocular pressure variation according to postconceptional age, and means and 10^th and 90^th percentiles were calculated for the intraocular pressure values.

RESULTS:

Fifty preterm infants with a mean gestational age of 29.7±1.6 weeks and a mean birth weight of 1,127.7±222.7 g were evaluated. The mean intraocular pressure for the entire cohort considering both eyes was 14.9±4.5 mmHg, and 13.5% of all recorded intraocular pressure values were greater than 20 mmHg. The analysis revealed a mean reduction in the intraocular pressure of 0.29 mmHg for each increase in postconceptional age (p = 0.047; 95% CI: −0.58 to −0.0035). The mean intraocular pressure (P10–P90) decreased from 16.3 mmHg (10.52–22.16) at 26.3 weeks to 13.1 mmHg (7.28–18.92) at 37.6 weeks of postconceptional age.

CONCLUSIONS:

The mean intraocular pressure in very low birth weight preterm infants was 14.9±4.5 mmHg. This value decreased 0.29 mmHg per week as the postconceptional age increased.     

促炎症细胞因子、T细胞亚群在小儿过敏性紫癜不同病期的变化

目的：探讨促炎症细胞因子（ＩＬ－６１、ＩＬ－８、ＴＮＦ－α）以及Ｔ细胞亚群在小儿过敏性紫癜发病中的作用。方法：采用双抗体夹心ＥＬＩＳＡ法检测３０例急性期２１例恢复期及２８例健康儿童血清及外周血单个核细胞（ＰＢＭＣ）培养上清液ＩＬ－６、ＩＬ－８及ＴＮＦ－α水平,以及外周血Ｔ细胞及其亚群的变化。结果：急性期血清及ＰＢＭＣ上清液ＩＬ－６、ＩＬ－８、ＴＮＦ－α水平明显高于恢复期和对照,且以ＩＬ－８、ＴＮＦ     

Comparison of T cell subsets between somatic cloned and normal cow

PROBLEM: Somatic cloning technology is beneficial for genetically producing excellent animals. However, many developmental problems of somatically cloned animals have been described. Some of them may cause disorders of the immune system, resulting in the fluctuation of the proportion of white blood cells (WBC), different from that of normal animals in peripheral blood. METHOD OF STUDY: In Holstein- cloned and normal cows, the fluctuation of granulocytes, monocytes, B cells and T cells, and further T cell subsets (CD4+, CD8+, gammadelta, CD8+gammadelta and WC1+gammadelta T cell) in peripheral blood were analyzed in early lactation stage (ELS) and mid to late lactation stage (MLS) by flow cytometry using specific monoclonal antibodies for cell surface markers. RESULTS: In both ELS and MLS, there were no significant differences in the proportions of granulocytes, monocytes, B cells and T cells between cloned and normal cows. In T cell subsets, gammadelta and WC1+gammadelta T cells in cloned cows were significantly less frequent than in normal cows in ELS. The decreased proportions of gammadelta and WC1+gammadelta T cells recovered to the level of normal cows in MLS. CONCLUSIONS: The population of granulocytes, monocytes, B cells and T cells, and T cell subsets except for gammadelta and WC1+gammadelta T cells in cloned cows fluctuated in a manner similar to those of normal cows during lactation. In ELS, the proportions of gammadelta and WC1+gammadelta T cells temporarily declined in cloned cows, suggesting that cloned cows may fall into an immunosuppressive state in ELS.     

Maternal and neonatal anti‐cytomegalovirus IgG level and risk of postnatal cytomegalovirus transmission in preterm infants

Immunological mechanisms influencing the risk of mother‐to‐child cytomegalovirus (CMV) transmission in preterm infants have not been studied sufficiently. In this study, the correlation between maternal and neonatal serum anti‐CMV IgG levels and risk of postnatal CMV transmission in preterm infants was assessed. Anti‐CMV IgG levels of 79 CMV seropositive mothers and their 94 infants were determined in peripheral blood samples collected within 3 days after delivery. Postnatal CMV infection was detected in 39/94 (41%) infants by PCR on urine at term‐equivalent age (gestational age 40 weeks) after congenital infection was excluded. Maternal or infant anti‐CMV IgG levels were not significantly different between infants with and without postnatal CMV infection. The anti‐CMV IgG infant–mother ratio showed a significant positive correlation with gestational age (range 25–32 weeks, R² = 0.218, P < 0.001), reaching 1.0 at 32 weeks of gestation. Anti‐CMV IgG infant–mother ratio was significantly lower in infants with postnatal CMV infection (P = 0.015). In conclusion, the risk of postnatal CMV transmission is related to low gestational age and low anti‐CMV IgG infant–mother ratio. J. Med. Virol. 85:689–695, 2013. © 2013 Wiley Periodicals, Inc.     

Galectin-3 in cord blood of term and preterm infants

In recent years galectin-3 has gained attention as a signalling molecule, mainly in inflammatory diseases. Data on galectin-3 expression in neonates, however, are limited, and expression of this lectin in cord blood has not yet been reported. The aim of this study was to determine galectin-3 levels in cord blood of term and preterm neonates as well as galectin-3 levels in cord blood of term neonates after stimulation with the prevalent pathogen Streptococcus agalactiae. Cord blood samples were incubated for 24 h and galectin-3 levels were assessed by enzyme-linked immunosorbent assay. There is a positive correlation between gestational age and galectin-3 levels in cord blood. Expression of galectin-3 is significantly higher in cord blood of small-for-gestational-age infants compared to appropriate-for-gestational-age infants. Stimulation with an invasive but not with a colonizing strain of S. agalactiae induced expression of galectin-3. Galectin-3 is expressed constitutively in cord blood of neonates and seems to play a role in the innate immunity of this population.     

The characterization and function of human immunoregulatory T lymphocyte subsets

Functional T lymphocyte subpopulations can be identified in humans by antibodies which detect stable glycoprotein antigens on their surface. Thus, inducer T lymphocytes bear an antigen termed T4 while suppressor T lymphocytes bear an antigen termed T5. Immune homeostasis results from a delicate balance between inducer and suppressor subsets within the T-cell circuit and perturbation in subset dynamics may initiate a wide variety of immunopathological disorders. Here Ellis Reinherz and Stuart Schlossman discuss the present understanding of this circuit, its role in the pathogenesis of a number of diseases and how the human immune response can be manipulated in an orderly way through modulation of selected T-cell subsets.     

慢性乙肝病毒携带者外周血皮质醇水平与T细胞亚群状态的研究

目的 探讨慢性乙肝病毒(HBV)携带者血浆皮质醇水平与细胞免疫状态的变化.方法 选取符合诊断标准的慢性HBV携带者60例,正常对照10例,放射免疫法测定血浆皮质醇水平,流式细胞仪检测外周血T细胞亚群CD3+、CD4+、CD8+、CD4+/CD8+比例.结果 与正常对照组相比,慢性HBV携带者血浆皮质醇水平相对升高(P＜0.05),CD4+细胞比例无明显变化(P＞0.05),CD8+细胞比例明显增加(P＜0.05),CD4+/CD8+明显下降(P＜0.05).HBeAg阳性组与HBeAg阴性组比较,HBeAg阳性组血浆皮质醇升高较为明显(P＜0.05),CD4+细胞比例无明显变化(P＞0.05).CD8+细胞比例明显增加(P＜0.05),CD4+/CD8+明显下降(P＜0.05).结论 慢性乙肝病毒携带者存在着血浆皮质醇水平的增高及细胞免疫功能的失衡,慢性HBV感染过程中存在内分泌-免疫系统紊乱.     

Comparison of postnatal development of heart rate responses to trigeminal stimulation in sleeping preterm and term infants

Autonomic dysfunction has been regarded as a possible cause of the sudden infant death syndrome (SIDS) and it has been suggested that preterm infants, who are at a greater risk of SIDS than term infants, may have immature autonomic control. Our aim was to compare the maturation of cardiac autonomic control during sleep in preterm and term infants by examining heart rate responses to arousing and non-arousing trigeminal stimuli. Preterm infants (n = 15) and term infants (n = 24) were studied longitudinally with daytime polysomnography. Air-jet stimulation of the nares was delivered in both active sleep (AS) and quiet sleep (QS), and heart rate (HR) changes recorded for both arousal and non-arousal responses. Changes in HR (DeltaHR%) were calculated as the relative differences between baseline HR (BHR) and either MaxHR (arousal) or MinHR (non-arousal). Comparisons of HR changes between sleep states and postnatal ages were made with two-way anova for repeated measures and between groups with two-way anova. The increase in HR (DeltaHR%) was greater in term than preterm infants (P < 0.05), but only at 2-3 weeks corrected postnatal age (CPA). In preterm infants, there were no differences in BHR between sleep states, whereas in term infants, BHR was higher in AS than in QS at 2-3 weeks and 2-3 months of age. The smaller DeltaHR% to arousing stimuli in preterm infants compared with term infants at 2-3 weeks suggests that cardiac sympathetic activity in preterm infants may be lower than in term infants. This mechanism may account for the increased risk for SIDS of preterm infants.