豚鼠钩体病PDH模型血浆栓素和前列腺环素的测定

Guinea pigs were intravenously injected with icterhemorrhagiae serogroup Lai serovar strain 017 leptospirosis to model the pulmonary diffuse hemorrhage (PDH) in leptospirosis. Thirty-eight hours after the injection, the jugular arteries were catheterized to collect blood sample. The plasma was prepared for radioimmunoassay of TXB2 and 6-keto-PGF1a, the stable metabolites of TXA2 and PGI2 respectively. The plasma level of TXB2 in the experimental group, 107.15 +/- 41.65 pg/ml (n = 7), almost doubled that of the control, 54.05 +/- 12.93 pg/ml (n = 7), with significant difference (P less than 0.01); meanwhile, no significant difference was observed of 6-keto-PGF1a, 67.97 +/- 16.89 pg/ml (n = 6) vs. 98.06 +/- 40.63 pg/ml (n = 9) with P greater than 0.1. The fact that TXA2 causes vasoconstriction and increases vessel permeability suggests that TXA2 elevation should play a role in the mechanism of PDH in leptospirosis.     

Effects of Codonopsis pilosulae on the synthesis of thromboxane A2 and prostacyclin

24 angina pectoris patients were treated with Codonopsis pilosulae (CP) oral solution 20 ml (containing crude CP 20 g) thrice daily for 7 days, other 10 cases were treated by aspirin 0.5 g per day for a week as the control group. After treatment, in the CP group, the plasma level of TXB2 was obviously reduced from 156.76 +/- 11.87 pg/ml to 125.01 +/- 8.85 pg/ml (means +/- S means), the inhibitory rates was 15. 67% (P less than 0.05), and of 6-keto-PGF1 alpha (6-K) was not markedly changed (P greater than 0.05). In the aspirin group, TXB2 was also reduced significantly (P less than 0.05); 6-K was reduced more than that of CP group, the inhibitory rate was 24.33 +/- 9.40% (P less than 0.05). To reveal the mechanism of CP action on the synthesis of TXA2 and PGI2, the porcine lung microsome was used as the donor of cyclooxygenase, thromboxane synthase and prostacyclin synthase, the effects of CP on the formation of TXB2 and 6-K from arachidonic acid (AA) or endoperoxides were measured by RIA respectively. The results showed that both the levels of the formation of TXB2 from AA or endoperoxides were markedly reduced by CP in a dose-dependent (at doses of 3-300 mg/ml). The synthesis of TXB2 was distinctly inhibited alone with a dose of 100 mg/ml CP, which suggested that CP might be an inhibitor of TXB2 synthase at that dose; while at a dose of 300 mg/ml CP, the synthesis of TXB2 and 6-K were inhibited simultaneously (P less than 0.001). It showed that a larger dosage of CP, which could inhibited the synthesis of both TXA2 and PGI2, its mechanism of action needs further study.     

Effect of jiang-zhi zhong-yao-pian on total cholesterol, triglyceride, TXB2, 6-keto-PGF1 alpha in hyperlipemic patients

The purpose of this study was to verify the effect of a Chinese herbal medicine Jiang-Zhi Zhong-Yao-Pian to reduce serum lipoid. Efficacy was observed in 30 cases of hyperlipemia; 20 cases administered with evening primose oil capsules were taken as controls. Each group took drugs for two or three months. The results were as follows: After treatment as compared with before treatment, the serum levels of TC, TG and TXB2 dropped from 264.28 +/- 70.52 mg%, 393.52 +/- 250.42 mg% and 110.75 +/- 43.52 pg/ml to 225.60 +/- 50.93 mg%, 264.97 +/- 252.81 mg% and 88.82 +/- 46.50 pg/ml respectively (P less than 0.001, less than 0.01, less than 0.05). However, in the group taking evening primrose oil capsules, TC, TG and TXB2 in comparing with the pre-treatment levels were changed from 251.33 +/- 58.24 mg%, 316.35 +/- 104.93 mg% and 131.53 +/- 49.77 pg/ml to 244.30 +/- 43.28 mg%, 272.10 +/- 92.52 mg% and 115.33 +/- 47.49 pg/ml respectively (P greater than 0.05, less than 0.05, greater than 0.05). This medicine had no side-effect. The results showed that the herbal formula might be useful to reduce serum TC, TG and TXB2.     

Superficial tongue blood volume and the tongue spectrophotometric determination on coronary heart disease with blood stasis syndrome

The tongue bodies of coronary heart disease (CHD) patients with blood stasis syndrome were looking dull purple in different degree. The authors adopted two kinds of biophysics methods which are tongue spectrophotometer and DM-80-6 superficial tongue blood volume meter. 52 CHD patients with blood stasis syndrome, 50 normal individuals and 26 patients before and after the treatment according to promote blood circulation to remove the blood stasis, were examined. The results were as follows: The purple ray values of the tongue spectrum of the CHD patients with blood stasis syndrome were more than that of normal individuals. Statistics showed significant difference (P less than 0.01). So was the tongue blood volume (P less than 0.01). In the treatment group, the purple ray value of tongue spectrum reduced, and the red ray volume of tongue increased significantly, as well (P less than 0.01). The results suggested that both methods may be quantitative comparative parameters for diagnosis, differentiation of symptoms and signs, and evaluation of the therapeutic effectiveness.     

Relation between changes of TXB2 and PGF1 alpha and the severity of the disease and pathological lesions in cases of severe icteric hepatitis.

Clinically, 32 cases of intrahepatic cholestasis and 30 cases of severe chronic hepatitis with a bilirubin level higher than 171 mumol/L were studied. The results showed that bilirubin was 420 mumol/L in the first group, and 480 mumol/L in the second group (P greater than 0.05); TXB2 was 306 pg/ml and 271 pg/ml (P greater than 0.05) while PGF1 alpha was 253 pg/ml and 494 pg/ml (P less than 0.05) respectively, both were higher than their normal values (P less than 0.01). The cases were divided into acute, moderate and severe types according to their pathological lesions. Experimentally, intra- and extrahepatic cholestasis and necrotic liver tissues were induced by ANIT, ligation of common bile duct, and carbon tetrachloride respectively. Bilirubin was 629 mumol/L, 124.8 mumol/L, and lower than 17.1 mumol/L (P less than 0.01); plasma TXB2 was 634 pg/ml, 1036 pg/ml, and 239 pg/ml (P less than 0.01); PGF1 alpha was 186 pg/ml, 218 pg/ml, and 868 pg/ml (P less than 0.01) in the three groups respectively. No statistic difference was found in their TXB2 and PGF1 alpha. Our studies suggested that plasma TXB2 and PGF1 alpha in the liver was not related to the severity of liver lesions. TXB2 and PGF1 alpha are positively correlated with the increase of bilirubin while TXB2 is negatively correlated with PGF1 alpha, which might serve as an index for cholestasis, and be a cause for deepening jaundice.

     

Clinical Implication of the Changes of cAMP, TXA_2 and PGI_2 in CSF of Asphyxiated Newborns

Summary:To evaluate the changes of 3',5'-cyclic adenosine monophosphate(cAMP),thromboxane A_2(TXA_2)and prostacyclin(PGI_2)in cerebrospinal fluid(CSF)in the asphyxiated newborn andexplore their roles in hypoxic-ischamic brain damage(HIBD).Thirty-six full term newborns were di-vided into 3 groups,including 12 with moderate-severe hypoxic-ischaemic encephalopathy(HIE),13with mild HIE,11 without HIE(control group).The levels of cAMP,TXB_2(TXA_2 metabolite)and6-keto-PGF_(1α)(PGI_2 metabolite)in CSF and plasma were measured 36—72h after birth by RIA.andthe concentrations were expressed as nM/L(cAMP),ng/L(TXB_2 and 6-keto-PGF_(1α)).The infantswere followed-up at 6 and 12 month of age and Mental Development Index(MDI)and PsychomotorDevelopment Index(PDI)were measured using Bayley Scales of Infant Development(BSID).TheCSF cAMP level in moderate-severe HIE group was 8.60±2.40,significantly lower than that of themild HIE group(14.83±2.84)and the control group(24.43±2.39)(for both P<0.01).The lev-els of TXB_     

An analysis of blood cell pouring on the tongue surface in 3032 healthy persons

This paper deals with the utilizing laser frequency multiplexing technique and Doppler effect, successfully to build up LDB-1 type of laser microcirculation blood flow meter. It was used to carry on a determination for blood cell pouring amount on tongue surface in 3032 healthy persons. The results were shown as follows. The average tongue blood cell pouring amount of healthy persons was 4.74 +/- 0.50, in which the male was 4.82 +/- 0.50, and the female was 4.66 +/- 0.49. Apparently, there was a great difference between them (P less than 0.01). Following the increase of age, the tongue blood cell pouring amount was gradually decreased. The average blood cell pouring amount of a group of 1-9 years old was 6.14 +/- 0.79, of over 60 years old was 3.89 +/- 0.39 (P less than 0.01). In the light red tongue, the average tongue blood cell pouring amount was 4.95 +/- 0.93, which was considered to be the highest; in the purple tongue, it was 3.96 +/- 0.44 which was considered the lowest. The average pouring amount in the red tongue was 4.94 +/- 0.77, and in the light white tongue was 4.21 +/- 0.52. The more red the tongue showed, the greater the tongue blood cell pouring amount would be; whereas, the more purple the tongue showed, the lower the tongue blood cell pouring amount would be. It obviously indicated that the tongue blood cell pouring amount would be. It obviously indicated that the tongue blood cell pouring amount could correctly reflect the different tongue characters and tongue blood circulation condition.     

Clinical and experimental studies of coronary heart disease treated with yi-qi huo-xue injection

The purpose of the present work was to evaluate the clinical efficacy and the mechanism of Yi-qi Huo-xue Injection (YHI) in treatment of coronary heart disease. YHI consists of Ginseng, Astragalus and Angelicae Sinensis. The 10% dextrose serves as a placebo. The results were as follows: 1. the frequency and severity of angina episodes were reduced by 90.63%; 2. the ischemic ST-T in ECG was improved in 56.25% of cases; 3. the tolerance to treadmill exercise was increased from 348.50 to 503.50 M.; 4. the left ventricular function was strengthened, PEP/LVET ratio reduced from 0.45 to 0.36, the activity of (Na(+)-K+) ATPase in myocardial cell membrane of rats inhibited by 19.2%; 5. the blood viscosity and erythrocyte electrophoretic time lowered; 6. the adhesion and aggregation of platelet in patients with CHD were inhibited by 27% and 59.4% respectively; 7. the plasma TXB2 level in CHD was reduced from 260.28 +/- 164.4 to 139.29 +/- 57.01 pg/ml; 8. the plasma 6-keto-PGF1 alpha level in CHD was increased from 33.45 +/- 22.5 to 57.48 +/- 13.1 pg/ml, and in rats from 185.77 to 366.33 pg/ml. The differences were all statistically significant (P less than 0.05-0.01) in comparison with the placebo group.     

The influence of sodium ferulate on hypotensive effect and urinary excretion of TXB2 after captopril in essential hypertensive patients]

In the present study, the influence of sodium ferulate (SF) on hypotensive effect and urinary excretion of TXB2 after captopril (CAP) was observed in 44 patients with essential hypertension. A single oral dose of CAP (50 mg) decreased mean arterial pressure (MAP) from 16.25 +/- 0.85 to 13.65 +/- 1.14 kPa, n = 28, (P less than 0.01), and increased urinary TXB2 excretion significantly from 119.12 +/- 57.12 to 183.32 +/- 78.61 pg/min, n = 16, (P less than 0.05). The administration of SF 300 mg/d for one day did not affect the MAP. CAP in combination with SF induced a decrease both in MAP from 16.33 +/- 1.14 to 13.83 +/- 1.77 kPa, n = 16, (P less than 0.01) and urinary TXB2 excretion from 155.89 +/- 69.64 to 133.43 +/- 60.01 pg/min, n = 16, (P greater than 0.05) though the latter was not so significant. Compared with the administration of CAP alone, the combination of CAP and SF induced stronger hypotensive effect (P less than 0.05) and the increased urinary TXB2 excretion could be inhibited by SF, but the inhibition to angiotensin converting enzyme was the same. These results suggested that the increased urinary TXB2 excretion by CAP can be inhibited and the hypotensive effect of CAP is potentiated by SF in essential hypertensive patients.     

Effects of Medicinal Cake-Separated Moxibustion on Plasma 6-Keto-PGF1α and TXB2 Contents in the Rabbit of Hyperlipemia

Hyperlipemia rabbit models established with high cholesterol and fat diet were treated vith directmoxibustion and medicinal cake-separated moxibustion. The post-treatment plasma 6-keto-prostaglandin F_(1α)(6-keto-PGF_(1α)) and thromboxane B2 (TXB_2) contents were determined by radioimmunoassay. Resultsindicated that the plasma 6-keto-PGF_(1α) content significantly increased,the TXB_2 level decreased (P<0.05)and the TXB_2 /6-keto-PGF_(1α) ratio also decreased (P<0.01) in the medicinal cake-separated moxibustiongroup as compared with those in the model group respectively,but there was no significant differencebetween the medicinal cake-separated moxibustion group and the direct moxibustion group (P>0.05),suggesting that both the medicinal cake-separated moxibustion and direct moxibustion can regulate theplasma 6-keto-PGF_(1α) and TXB_2 contents,and the TXB_2/6-keto-PGF_(1α) ratio with similar actions,and have acertain protective action on endothelial cells of the aorta in the rabbit of hyperlipemia     

高压氧对激素性股骨头坏死兔血清TXA2、PGI2及血液流变学的影响

目的通过观察高压氧（HBO）治疗前后激素性股骨头坏死（SANFH）兔血清血栓素A2（TXA2）、前列腺素I2（PGI2）及血液流变学指标的变化,探讨HBO治疗SANFH的作用机制。方法健康新西兰白兔正常对照组10只、模型组20只[非干预组（N-HPO）和HPO组各10只],正常对照组和N-HPO组不予任何处理,高压氧组予以高压氧疗。检测并比较各组血清血栓素B2（TXB2,TXA2的稳定代谢产物）、6-酮-前列腺素F1α（6-keto-PGF1α,PGI2的稳定代谢产物）含量及血液流变学的变化。结果建模前模型组和对照组的血清TXB2、6-keto-PGFlot和血流变指标无明显差异（均P〉0．05）,建模成功后模型组的TXB2和血液流变学指标均显著高于对照组,而6-keto-PGF1α显著低于对照组,差异有统计学意义（均P〈0．01）;治疗前HPO组和N-HPO组的血清TXB2、6-keto-PGF1α和血液流变学指标无明显差异（均P〉0．05）,HPO治疗后2周、4周和6周模型组的TXB2和血液流变学指标均显著低于非HPO组,而6-keto-PGF1α显著高于非HPO组,差异有统计学意义（均P〈0．01）。结论HBO可纠正SANFH兔血中TXAJPGl2失衡,降低血黏度,减轻股骨头损伤。     

基于色度学的舌色分类研究现状与分析

目的探讨基于色度学的舌色分类研究现状,为数字舌图的舌色分类研究提供依据与参考。方法回顾性分析22项舌色分类研究的数据,将舌色的色度值统一转化为L值、a值、b值、C值、H值,进行各类舌色的色度值比较,并观察各类舌色在国际照明委员会(CIE)提出的颜色-对立空间模型(CIELAB)和明度彩度色调颜色模型(CIELCH)的分布,采用K均值聚类的方法,对不同研究者报道的舌色色度值进行聚类分析,并作色差比较。结果 22篇文章共报道了11类舌色:淡白舌、淡红舌、红舌、红绛舌、暗红舌、淡紫舌、紫红舌、青紫舌、紫舌、绛紫舌、紫暗舌。11类舌色的亮度在45～60之间,色相角在8°～27°和320°～355°之间,饱和度在17～36之间。从淡白舌到淡红舌、红舌、红绛舌、暗红舌,观察到有规律的色度值变化,表现为L值逐渐下降,而a值逐渐升高的趋势。以聚类中心位置的色度值为比较的基准,色差的范围在2.44～19.70之间。结论色度学为中医将舌诊的色觉经验进一步升华为量化色诊提供了关键的理论与技术。但不同研究者报道的色度值差异较大,也提示应当尽快制定数字舌图采集与色彩校正的规范方案与流程,使获得的舌色色度值能够在公共平台进行有效交流。     

选择性环加氧酶2抑制剂对糖尿病大鼠肾脏的影响及其作用机制研究

目的　探讨选择性环加氧酶 2抑制剂莫比可对糖尿病大鼠肾脏病变的影响。方法　将大鼠分成正常对照组 (6只 )、不用药组 (8只 )、消炎痛组 (6只 )和莫比可组 (9只 )。 16周后放射免疫法测定大鼠尿液中前列腺素E2 (PGE2 )和血栓烷素B2 (TXB2 )的排泄量 ,应用逆转录 聚合酶链式反应和免疫沉淀的方法分别检测肾皮质中转化生长因子 β1(TGF β1)及其Ⅱ型受体 (TβR2 )的基因表达和血管紧张素Ⅱ 1型受体 (AT1R)的蛋白水平 ,并观察PAS染色下肾脏的病变情况。结果　与正常对照组相比 ,糖尿病大鼠PGE2 和TXB2 排泄增多 (分别为 16 4 1pg/ 2 4h± 2 88pg/ 2 4h ,5 5 0 7pg/ 2 4h± 135 9pg/ 2 4h)。TGF β1和TβR2 的基因表达显著上调 ,分别为 0 185± 0 0 37,0 194± 0 0 5 4。AT1R的蛋白水平下降 2 1 3%。光镜显示肾小球系膜区增宽 ,PAS染色阳性的胶原沉积增多。消炎痛、莫比可均能不同程度地减少PGE2 的生成 (P <0 0 5 ) ,但TXB2 仅在莫比可组显著下降。莫比可组TGF β1和TβR2 基因表达明显下调 (39% ,4 7% ) ,AT1R的蛋白水平回升 (P <0 0 5 ) ,肾脏病变有所好转 ,而消炎痛无效。结论　莫比可对糖尿病肾脏病变具有一定的保护作用 ,其机制可能与调节TGF β1和AT1R有关     

肝阴虚证患者血浆血栓素A2、前列环素I2水平研究

为了比较慢性肝炎与其它疾病中肝阴虚证血浆血栓素B2（TXB2）和6－酮－前列腺素F1α6－Keto-PGF1α水平,用放射免疫法检测28例肝阴虚证患者血浆TXB2和6－Keto-PGF1α含量,又分慢性肝炎与其它疾病两组进行对比观测。结果表明,肝阴虚证患者血浆TXB2水平、T／6－K值显著高于健康对照组（均P＜0．01）,6－Keto-PGF1α水平显著低于健康对照组（P＜0．05）,慢性肝炎组与其它疾病组TXB2、T／6－K值均显著高于健康对照组（均P＜0．01）、6－Keto-PGF1α显著低于健康对照组（均P＜0．01）,且两组组间比较差异无显著性（P＞0．05）。提示肝阴虚证患者调节血管平滑肌舒缩功能的活性物质紊乱,存在微循环障碍。     

肺缺血再灌注损伤时TXA2/PGI2平衡的变化及尼美舒利对其的影响

目的：探讨大鼠肺缺血再灌注损伤时TXA2/PGI2的变化及尼美舒利对其的影响。方法：健康SD大鼠30只,随机分为假手术组（S组）、缺血再灌注组（I/R组）和尼美舒利组（NIM组）,复制在体大鼠肺缺血再灌注损伤模型。检测血清丙二醛（MDA）含量、超氧化物歧化酶（SOD）和黄嘌呤氧化酶（XO）活力,放射免疫法测定肺组织血栓素B2（TXB2）、6-酮-前列腺素F1α（6-keto-PGF1α）含量并计算比值;逆转录聚合酶链反应（RT-PCR）检测肺组织环氧合酶-2 mRNA（COX-2 mRNA）的表达。结果：NIM组与IR组相比,血清MDA、XO均明显降低,SOD明显升高;TXB2明显下降（均P〈0.01）,6-keto-PGF1α无明显差异,COX-2 mRNA表达明显减弱（P〈0.01）。结论：尼美舒利可通过下调肺组织COX-2 mRNA的表达,抑制血小板释放TXA2,调控TXA2/PGI2的平衡,增强抗氧化应激而减轻肺缺血再灌注损伤。     

灯盏花素治疗重症急性胰腺炎临床研究

目的：观察灯盏花素治疗重症急性胰腺炎（Severe acute pancreatitis,SAP）的作用。方法：将40例SAP患者随机分为A组（20例）和B组（20例）,同时以健康志愿者20例作为正常对照组。A组采用常规治疗,B组采用常规治疗＋灯盏花素注射液治疗,以放射免疫法测定血浆ET、TXB2、6-keto-PGF1α浓度及TXB2/6-keto-PGF1α比值,测定A、B两组APACHEⅡ评分及胰腺Balthazar CT分级评分。结果：（1）血浆6-keto-PGF1α、ET、TXB2及TXB2/6-keto-PGF1α比值：SAP患者血浆6-keto-PGF1α浓度显著低于正常对照组,而血浆ET、TXB2浓度及TXB2/6-keto-PGF1α比值显著高于正常对照组（P〈0.01）;B组入院后8天与B组入院时及A组入院后8天比较,血浆6-keto-PGF1α浓度显著上升,而血浆ET、TXB2浓度及TXB2/6-keto-PGF1α比值,均显著下降（P〈0.01～0.05）。（2）APACHE II评分和胰腺Balthazar CT分级评分：A、B两组入院后8天与入院时比较,两评分均显著下降（P〈0.01～0.05）;B组入院后8天与A组入院后8天比较,两评分均显著降低（P〈0.01～0.05）。结论：灯盏花素可能通过改善SAP患者的微循环障碍,从而对SAP起治疗作用。     

替普瑞酮对类固醇致胃黏膜损伤的保护作用

目的研究替普瑞酮对类固醇激素所致胃黏膜损伤的保护作用及其机制。方法50只雄性SD大鼠随机分为空白组、实验对照组、低剂量替普瑞酮组、中剂量替普瑞酮组和高剂量替普瑞酮组,每组10只。采用泼尼松龙皮下注射制备大鼠胃黏膜损伤模型,低、中、高剂量组替普瑞酮的剂量分别为50、100、200mg/kg,给药7d,每天1次。观察胃黏膜的病理变化,计算溃疡指数、胃黏膜组织学损伤指数,放射免疫法检测血浆内皮素1和胃黏膜前列腺素E2(PGE2)水平,Griess法检测血清NO含量。结果类固醇激素能引起胃黏膜显著出血性损伤,实验对照组大鼠溃疡指数中位数为44.5,组织学损伤指数中位数为5.5,明显高于空白组(均为0,均P<0.01);实验组大鼠血浆内皮素1水平为399pg/ml±74pg/ml,高于空白组(279pg/ml±56pg/ml,P<0.01);血浆NO水平(27μmol/L±5μmol/L)低于空白组(36μmol/L±5μmol/L,P<0.01);胃黏膜PGE2水平(154pg/mg±83pg/mg)低于空白组(337pg/mg±112pg/mg,P<0.01)。低、中、高剂量替普瑞酮组的溃疡指数中位数分别为32.5,23.0,23.0,均明显低于实验组(均P<0.01);组织学损伤指数中位数分别为3.0,3.0,1.5,均明显低于实验组(均P<0.01),内皮素1水平分别为299pg/ml±99pg/ml,284pg/ml±85pg/ml,189pg/ml±32pg/ml,均明显低于实验对照组(P<0.05,P<0.01,P<0.01);NO水平分别为56μmol/L±16μmol/L,62μmol/L±12μmol/L,83μmol/L±9μmol/L,均明显高于实验对照组(均P<0.01),高剂量替普瑞酮组胃黏膜PGE2水平为241pg/mg±65pg/mg,明显高于实验组154pg/mg±83pg/mg(P<0.05)。溃疡指数、组织学损伤指数和内皮素1水平随替普瑞酮剂量的增大而降低,血清NO、胃黏膜PGE2水平随替普瑞酮剂量的增大而升高。结论替普瑞酮对类固醇致胃黏膜损伤具有一定的保护作用,其机制可能与降低内皮素1水平和增加NO和PGE2生成有关。     

The influence of insulin on secretion of IGF-Ⅰ and IGFBP-Ⅰ in cultures of human endometrial stromal cells

Objectives To study the influence of insulin on IGF-Ⅰ and IGFBP-Ⅰ secretion of the human endometrial stromal cells. Methods Late proliferative phase endometrial stromal cells were isolated from endometrium tissues and then cultured for 24 h in Hams F-12 only as a control and in Hams F-12 with different concentrations of estradiol (E2) and insulin (INS) as treated groups. Simultaneously, the endometrial stromal cells from late secretory phase endometrium were cultured for 24 h in Hams F-12 only as a control and in Hams F-12 supplemented with different concentrations of progesterone (P) and insulin as treated groups. After 24 h of culturing, the mediums were collected for either IGF-Ⅰ or IGFBP-Ⅰ assays. Result The concentrations of IGF-Ⅰ in medium from cultured endometrial stromal cells in the proliferative phase were 0.78±0.47 ng/ml in the hormone-free control group; 1.44±0.59 ng/ml and 1.39± 0.33 ng/ml in 100 pg/ml E2 group and 20 μU/ml INS group, which was higher than that of the control group (P&lt;0.05 and P&lt;0.01, respectively). The IGF-Ⅰ concentration in the 100 μU/ml INS group was 2.03±0.53 ng/ml, which was higher than that of the 20 μU/ml INS group (P&lt;0.01). Levels of IGF-Ⅰ in the 100 pg/ml E2 plus 20 μU/ml INS group was 2.18±0.36 ng/ml, which was significantly higher than that of the 20 μU/ml INS and 100 pg/ml E2 group (P&lt;0.01), but lower than that of the 100 pg/ml E2 plus 100 μU/ml INS group (3.42±0.75 ng/ml), P&lt;0.01. The concentration of IGFBP-Ⅰ in medium from cultured endometrial stromal cells in the secretory phase was 2.50±1.39 ng/ml in the hormone-free control group and 5.44±2.09 ng/ml in the 10 pg/ml P group, which was significantly higher than that of the control (P&lt;0.01). IGFBP-Ⅰ concentration in 20 μU/ml INS group was 0.16±0.58 ng/ml, which was lower compared with control, but higher compared with the 100 μU/ml INS group (P&lt;0.01). The level of IGFBP-Ⅰ in the 10 ng/ml P plus 20 μU/ml INS group was 2.10±1.17 ng/ml, lower compared with the 10 ng/ml P group, but higher compared with the 10 pg/ml P plus 100 μU/ml INS group, P&lt;0.01. Conclusions Insulin can stimulate basal (without hormone) and E2-stimulated IGF-Ⅰ secretion in cultured stromal cells from human late proliferative endometrium in a dose-dependent manner. Insulin can suppress basal (without hormone) and P-stimulated IGFBP-Ⅰ secretions in cultured stromal cells from human secretory endometrium in a dose-dependent manner.     

Relation of tongue color changes and red-cell immune adherence activity in patients with malignant bone tumor

This article presents that preliminary study on the changes of the tongue colour and the red-cell immune adherence (RCIA) activity in 40 patients with malignant bone tumor, as compared with 40 healthy persons. The results showed that the rate of erythrocyte C3b receptor yeast rosette were no statistically significant different between the pink and the crimson tongues in the bone tumor group and control group (P greater than 0.05). It appeared RCIA activity was no depression in the patients with malignant bone tumor during pink and crimson tongue. In the bone tumor group with the pale and the cyanotic tongues, the rate of erythrocyte C3b receptor yeast rosette and erythrocyte immune complex rosette was significantly lower than that of the control group (P less than 0.05, P less than 0.01). It appeared that RCIA activity in these patients was markedly impaired. The authors suggested that the tongue colour changes in the patients with malignant bone tumors could roughly reflect the level of RCIA activity. This phenomenon is beneficially to predict the body capacity against the malignant tumor.