大蒜素保留灌肠治疗溃疡性结肠炎临床研究

目的观察大蒜素保留灌肠在溃疡性结肠炎治疗中的疗效,副作用及半年复发率。方法随机将溃疡性结肠炎患者分为两组,治疗组50例,给大蒜素液40 mg,山莨菪碱10 mg,生理盐水100 ml,保留灌肠,每晚睡前1次;对照组40例,给予柳氮磺胺吡啶1 g,4次/d,复方谷氨酰胺0.2 g,3次/d,餐后服用。均治疗4周。治疗4周后复查有关生化指标,血尿便三大常规及肠镜,并随访半年。结果显效率、总有效率及半年复发率,治疗组分别为48.0%,86.0%和18.0%;对照组分别为30.0%,72.5%,35.0%。治疗组未见明显副反应,对照组副反应有白细胞轻度减少3例(7.5%),胃肠道反应9例(25.0%)。结论大蒜素保留灌肠能有效治疗溃疡性结肠炎,且副作用少,复发率低。     

CT诊断红斑狼疮性胃肠炎一例

患者女，35岁。因上腹痛伴呕吐。腹泻15d加重1d入院。体检：体温36．8℃，表浅淋巴结不大，四肢关节无肿痛，腹稍膨隆，肝脾末及，中上腹、左下腹轻压痛，无反跳痛，移动性浊音阳性（＋），肠鸣音减弱。入院10d内实验室检查：血尿常规、血沉、肝肾功能均正常，血清结核菌素试验明性。大便呈黄色水样，未见红、白细胞，未检出致病菌，潜血（一）。腹水呈淡黄色，性质介于渗出液和漏出液之间，未找到癌细胞。胃镜见胃窦部黏膜充血、水肿，活检为轻度慢性炎症。X线检查：胸片无异常，腹平片除腹水征象外未见异常，气钡双对比灌肠造影未见结肠…     

三氧化二砷对人结肠癌细胞增殖及凋亡影响的研究

目的：观察三氧化二砷（As2O3）对人结肠腺癌LS-174T细胞增殖的抑制及凋亡的诱导作用。方法：应用MTT法、细胞集落形成试验、流式细胞仪技术和TUNEL法观察不同浓度的As2O3(1.0μmol,2.0μmol，4.0μmol，8.0μmol，16.0μmol/L）对LS-174T细胞增殖和凋亡的影响。免疫组化技术观察As2O3对bcl-2基因表达的影响。结果：LS-174T细胞经As2o3作用后，细胞生长抑制率上升，细胞集落形成率下降，细胞周期中G1期细胞比例上升，S期和G2/M期细胞比例下降，TUNEL法显示凋亡细胞数增加，免疫组化结果显示bcl-2的表达明显减弱。结论：As2O3能抑LS-174T细胞增殖并诱导该细胞株凋亡，其机理可能与下调bcl-2表达有关。     

新生儿全结肠无神经节细胞症X线诊断

目的：探讨新生儿全结肠无神经节细胞症X线诊断。材料与方法：1985年至1995年经X线检查后手术病理证实此病者18例。结果：腹部平片17例显示：中上腹小肠胀气伴气液面15例，直肠少气或无气16例。无1例结肠胀气。钡灌肠18例显示：结肠细小5例。结肠宽径多数近似正常、但肠管形态多不正常（包括僵直9例，锯齿状改变7例），结肠短缩12例，直肠壶腹消失14例。排钡延迟11例。结论：钡灌肠对诊断本病有重要价值。     

同步放化疗治疗可手术食管癌的临床疗效分析

目的：探讨同步放化疗及单纯放疗治疗可手术食管癌患者的近期疗效、毒副反应及远期生存率。方法210例患者随机分成两组,紫杉醇＋顺铂方案化疗同步放疗组（同步组）109例,单纯放疗（单放组）101例。同步组：紫杉醇75 mg／m2 d1,8,顺铂20 mg／m2 d1－5,每4周重复,共化疗四个周期。放疗采用常规分割,照射剂量60－66GY／32Fx;单放组：仅行放疗,方法及剂量与同步组相同。结果同步组有效率90.83％;高于单放组的66.34％;比较差异有统计学意义（ P＜0.05）。毒副反应主要为骨髓抑制、放射性食管炎、外周神经毒性等。同步组Ⅱ～Ⅳ度白细胞减少,明显高于单放组（ P＜0.05）。其他毒副反应,两组比较差异无统计学意义（ P＞0.05）;同步组1 a生存率为78.3％,高于单放组的53.7％（ P＜0.05）。结论紫杉醇＋顺铂方案同步放疗治疗可手术食管癌近期疗效及1a生存率均显著提高,毒副反应可耐受。     

三氧化二砷诱导急性早幼粒白血病细胞凋亡的机制

目的 探讨线粒体在三氧化二砷(As2O3)诱导急性早幼粒细胞白血病NB4细胞凋亡中的作用并观察线粒体基因组在该过程中的表达变化.方法 采用荧光显微镜、流式细胞仪、细胞生长抑制实验、线粒体膜电位检测、RT-PCR等方法,观察As2O3对NB4细胞的诱导凋亡、生长抑制作用及线粒体基因差异表达变化.结果 荧光显微镜观察显示,NB4细胞经As2O3处理48h后呈现出明显的凋亡形态学改变.As2O3在一定的浓度范围内对NB4细胞具有显著的生长抑制效应.流式细胞仪分析发现,NB4细胞经0.5、1、2、3μmol/L As2O3处理后,其细胞凋亡率分别为5.02%、6.40%、28.40%、33.34%.以0.5、1、2、4、8μmol/LAs2O3分别处理NB4细胞48h后,流式细胞仪检测NB4细胞线粒体膜电位分别下降12.8%、21.6%、66.9%、83.7%、83.8%.对As2O3诱导NB4细胞凋亡过程中的13个线粒体基因组基因进行RT-PCR分析发现,COX2基因表达下调,其余12个基因表达无明显改变.结论 As2O3在体外对NB4细胞具有显著的诱导凋亡及生长抑制效应,其诱导凋亡作用与线粒体膜电位下降密切相关;线粒体相关基因COX2的表达变化参与了As2O3诱导的NB4细胞凋亡过程.     

新生儿全结肠无神经节细胞症X线诊断

目的：探讨新生儿全结肠无神经节细胞症X线诊断。材料与方法：1985年至1995年经X线检查后手术病理证实此病者18例。结果；腹部平片17例显示：中上腹小肠胀气伴气液面15例，直肠少气或无气16例。无1例结肠胀气。钡灌肠18例显示：结肠细小5例。结肠宽径多数近似正常、但肠管形态多不正常(包括僵直9例，锯齿状改变7例)，结肠短缩12例，直肠壶腹消失14例。排钡延迟11例。结论：钡灌肠对诊断本病有重要价值。     

胆总管热盐水灌注对犬胆管上皮及肝肾功能的影响

目的:观察胆总管灌注温热盐水对犬胆管和肝肾功能的影响。方法:8条犬DSA下穿刺胆囊胆总管内置入导管,42℃~43℃温热盐水灌注。术前及术后查肝肾功能,结果用单因素方差分析。处死动物,HE染色观察胆管病理变化。结果:灌注热疗后1周内,肝肾功能指标无显著变化(P>0.05)。光镜观察灌注局部胆管病理:3天可见淋巴细胞浸润,1周可见淋巴细胞增生,2周可见粘膜下腺体增生。未见粘膜上皮损伤。结论:经胆总管42℃~43℃温热盐水灌注15min,1周内对肝、肾功能无明显影响;2周内光镜观察未见胆管粘膜上皮损伤性改变。     

复方五倍子液保留灌肠治疗慢性结肠炎30例

我院子1993年3月～1994年1月用自制复方五倍子液保留灌肠治疗慢性结肠炎30例，疗效满意。资料与方法一、一般资料本组男21例，女9例，年龄24～72岁（平均40．2岁）。病程6个月～20年。病变在左半结肠者25例，全结肠者5例。全部病例用20％甘露醇准备肠道，每疗程结束后复查肠镜。二、诊断标准[1]（1）长时间腹痛、腹泻或便秘，大便常规检查无白细胞、脓细胞，细菌培养阴性；（2）肠镜检查见肠粘膜光滑，有充血、水肿，血管纹理增粗、紊乱，网状结构消失；（3）病理检查为非特异炎症。三、治疗方法方药组成：五倍子18g，黄连、黄柏、黄羊、元…     

伴有腹水的传染性单核细胞增多症1例

病儿，男，9岁。8d前无明显诱因发热，体温波动在38．5～39．2℃，用退热药后可暂时下降，发热时无畏寒、乏力。在外院予红霉素、头孢拉定、阿奇霉素等治疗8d，体温仍不退。1d前家长无意中发现患儿颈部有一肿块，无疼痛感。发病以来无咳嗽、腹泻，无皮疹、关节肿痛，食欲欠佳。个人史、家族史、预防接种史无特殊。入院查体：体温37．3℃，心率100次／min，体质量44kg，血压110／70mmHg(1mmHg=0．1333kg)，营养发育好，步入病房。     

Knee injury and Osteoarthritis Outcome Score (KOOS) - validation of a Swedish version

The Knee injury and Osteoarthritis Outcome Score (KOOS) is a self-administered instrument measuring outcome after knee injury at impairment, disability, and handicap level in five subscales. Reliability, validity, and responsiveness of a Swedish version was assessed in 142 patients who underwent arthroscopy because of injury to the menisci, anterior cruciate ligament, or cartilage of the knee. The clinimetric properties were found to be good and comparable to the American version of the KOOS. Comparison to the Short Form-36 and the Lysholm knee scoring scale revealed expected correlations and construct validity. Item by item, symptoms and functional limitations were compared between diagnostic groups. High responsiveness was found three months after arthroscopic partial meniscectomy for all subscales but Activities of Daily Living.     

Endovascular management of carotid-cavernous fistulas

Objective To investigate endovascular treatment of traumatic direct carotid-cavernous fistulas （CCF） and their complications such as pseudoaneurysms. Methods： Over a five-year period, 22 patients with traumatic direct CCFs were treated endovascularly in our institution. Thirteen patients were treated once with the result of CCF occluded, 8 twice and 1 three times. Treatment modalities included balloon occlusion of the CCF, sacrifice of the ipsilateral internal carotid artery with detachable balloon, coll embolization of the cavernous sinus and secondary pseudoaneurysms, and covered-stem management of the pseudoaneurysms. Results All the direct CCFs were successfully managed endovascularly. Four patients developed a pseudoaneurysm after the occlusion of the CCF with an incidence of pseudoaneurysm formation of 18.2% （4/22）. A total number of 8 patients experienced permanent occlusion of the ICA with a rate of ICA occlusion reaching 36.4% （8/22）. Followed up through telephone consultation from 6 months to 5 years, all did well with no recurrence of CCF symptoms and signs. Conclusion Traumatic direct CCFs can be successfully managed with endovascular means. The pseudoaneurysms secondary to the occlusion of the CCFs can be occluded with stent-assisted coiling and implantation of covered stents.     

The acutely limping child

Acute limping may be the result of multiple pathologies in children. The differential diagnosis varies based on the age of the child. Irrespective of age, the initial imaging work-up includes AP and frog leg radiographs of the pelvis and ultrasound; MRI may sometimes be helpful. In children less than 3 years, infections and trauma are most frequent. MRI is the imaging modality of choice when osteomyelitis is clinically suspected. Between the ages of 3 and 10 years, transient synovitis of the hip and Legg-Calvé-Perthes disease are main considerations but infection, inflammation and focal bony lesions are also considered. In children over 10 years, slipped capital femoral epiphysis also is considered.     

Do Balance Board Training Programs Reduce the Risk of Ankle Sprains in Athletes?

Introduction Ankle sprains are the most common musculo-skeletal injury that occurs in athletes,particularly in sports that require jumping and landing on one foot such as soccer,and basketball(1-4).These injuries often result in significant time loss from participation,long-term disability,and have a major impact on health care costs and resources(5-8).     

High Intensity Interval Training:New Insights

KEY POINTS ·High-intensity interval training(HIT)is characterized by repeated sessions of relatively brief，intermittent exercise.often performed with an“a11 out”effort or at an intensity close to that which elicits peak oxygen uptake(i.e.，≥90%of VO2 peak).     

Developmental validation of the PowerPlex(®) ESI 16 and PowerPlex(®) ESI 17 Systems: STR multiplexes for the new European standard

In response to the ENFSI and EDNAP groups’ call for new STR multiplexes for Europe, Promega^® developed a suite of four new DNA profiling kits. This paper describes the developmental validation study performed on the PowerPlex^® ESI 16 (European Standard Investigator 16) and the PowerPlex^® ESI 17 Systems. The PowerPlex^® ESI 16 System combines the 11 loci compatible with the UK National DNA Database^®, contained within the AmpFlSTR^® SGM Plus^® PCR Amplification Kit, with five additional loci: D2S441, D10S1248, D22S1045, D1S1656 and D12S391. The multiplex was designed to reduce the amplicon size of the loci found in the AmpFlSTR^® SGM Plus^® kit. This design facilitates increased robustness and amplification success for the loci used in the national DNA databases created in many countries, when analyzing degraded DNA samples. The PowerPlex^® ESI 17 System amplifies the same loci as the PowerPlex^® ESI 16 System, but with the addition of a primer pair for the SE33 locus. Tests were designed to address the developmental validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM), and those of the DNA Advisory Board (DAB). Samples processed include DNA mixtures, PCR reactions spiked with inhibitors, a sensitivity series, and 306 United Kingdom donor samples to determine concordance with data generated with the AmpFlSTR^® SGM Plus^® kit. Allele frequencies from 242 white Caucasian samples collected in the United Kingdom are also presented. The PowerPlex^® ESI 16 and ESI 17 Systems are robust and sensitive tools, suitable for the analysis of forensic DNA samples. Full profiles were routinely observed with 62.5 pg of a fully heterozygous single source DNA template. This high level of sensitivity was found to impact on mixture analyses, where 54–86% of unique minor contributor alleles were routinely observed in a 1:19 mixture ratio. Improved sensitivity combined with the robustness afforded by smaller amplicons has substantially improved the quantity of data obtained from degraded samples, and the improved chemistry confers exceptional tolerance to high levels of laboratory prepared inhibitors.