阵发性睡眠性血红蛋白尿患者的淋巴细胞对红系和粒—巨噬？…

为了研究阵发性睡眠性血红蛋白尿（ＰＮＨ）患者的淋巴细胞对红系和粒－巨噬系祖细胞的影响，将ＰＮＨ患者的植物血凝素－淋巴细胞培养液（ＰＨＡ－ＬＣＭ）加入造血祖细胞培养基中，用于红系爆式集落形成单位（ＢＦＵ－Ｅ）和粒－巨噬系集落形成单位（ＣＦＵ－ＧＭ）的培养。结果在含有ＰＮＨ患者的ＰＨＡ－ＬＣＭ的培养条件下，ＰＮＨ患者骨髓ＢＦＪ－Ｅ和ＣＦＵ－ＧＭ集落数明显低于含有正常人ＰＨＡ－ＬＣＭ的培养条件下的集落数     

阵发性睡眠性血红蛋白尿患者骨髓多向祖细胞的特点

采用造血祖细胞体外培养技术,研究阵发性睡眠性血蛋白尿（ＰＮＨ）患者骨髓多向祖细胞（ＣＦＵ－Ｍｉｘ）的增殖能力;骨髓细胞经酸化ＡＢ血清处理后ＣＦＵ－Ｍｉｘ的增殖能力。发现ＰＮＨ患者骨髓ＣＦＵ－Ｍｉｘ的集落数明显低于正常;骨髓细胞顷新鲜酸化ＡＢ血清处理后的ＣＦＵ－Ｍｉｘ集落数,明显低于经热酸化ＡＢ血清处理后培养的集落数。     

维甲酸对正常造血祖细胞集落形成的影响

用细胞培养方法观察了不同浓度（１０－９～１０－５ｍｏｌ／Ｌ）的全反式维甲酸（ＡＴＲＡ）对正常造血祖细胞集落形成的影响。结果表明，随着浓度的增加，ＡＴＲＡ对红系祖细胞集落形成单位（ＣＦＵ－Ｅ、ＢＦＵ－Ｅ）的抑制作用增加，但在１０－８～１０－６ｍｏｌ／Ｌ内对粒单系祖细胞集落形成单位（ＣＦＵ－ＧＭ）的形成有促进作用，１０－６ｍｏｌ／Ｌ的ＡＴＲＡ对巨核系祖细胞集落形成单位（ＣＦＵ－Ｍｅｇ）生长也有促进作用，提示在急性早幼粒细胞白血病（ＡＰＬ）用ＡＴＲＡ治疗过程中，ＡＴＲＡ参与了其正常造血功能的恢复。     

维生素A缺乏对红系早期祖细胞增殖影响的研究

采用造血祖细胞体外培养。造血生长因子生物活性检测等实验血液学技术，研究维生素Ａ缺乏（ＶＡＤ）对大鼠红系早期祖细胞（ＢＦＵ－Ｅ）增殖调控的影响。结果发现，与对照组比较，ＶＡＤ使ＢＦＵ－Ｅ集落增殖明显减少；用ＶＡＤ的脾及骨髓基质细胞培养上清液明显抑制ＢＦＵ－Ｅ集落增殖。结果提示，ＶＡＤ可能通过抑制造血生长因子白细胞介素３（ＩＬ－３）和粒单系集落刺激因子（ＧＭ－ＣＳＦ）等表达而影响红系造血。     

当归多糖对造血祖细胞增殖调控机理的研究

探讨当归多糖（ＡＰ）对造血祖细胞增殖调控和“补血”的生物学机理。方法采用贫血小鼠模型，用造血祖细胞体外培养和造血生长因子检测等技术，测定ＡＰ对造血祖细胞克隆增殖等影响。结果ＡＰ对正常或贫血鼠的髓系造血祖细胞（ＢＦＵ－Ｅ，ＣＦＵ－Ｅ，ＣＦＵ－ＧＭ和ＣＦＵ－ＭＫ）增殖分化有显著促进作用；经ＡＰ诱导制备的成纤维细胞、巨噬细胞、脾细胞和骨骼肌条件培养液能提高造血祖细胞集落产率。结论ＡＰ促进造血的机理可能与直接或间接刺激造血诱导微环境的巨噬细胞、成纤维细胞、淋巴细胞等分泌较高活性的造血生长因子有关。     

人参总皂甙对人血细胞生成的研究

从人参中提取有效成份人参总皂甙（ＴＳＰＧ），测定它对人骨髓粒单系祖细胞（ＣＦＵ－ＧＭ），红系祖细胞（ＣＦＵ－Ｅ，ＢＦＵ－Ｅ）和多向祖细胞（ＣＦＵ－ＧＥＭＭ）的刺激增殖作用。结果显示：在培养中加入不同浓度的ＴＳＰＧ５．２５，５０，７５，１００ｍｇ／Ｌ，可促进造血细胞增殖，在体外形成集落。在ＴＳＰＧ５－７５ｍｇ／Ｌ时，ＣＦＵ－ＧＭ集落产率均明显高于不加ＴＳＰＧ的对照组浓度的ＴＳＰＧ使集落产率明显高于对     

当归注射液对正常人和再生障碍性贫血患者造血祖细胞的 …

本文采用体外造血祖细胞集落形成技术，观察了当归注射液对正常人和再生障碍性贫血（再障）患者骨髓红系（ＣＦＵ－Ｅ，ＢＦＵ－Ｅ）、粒系（ＣＦＵ－ＧＭ）的刺激增殖作用。实验结果表明：在正常骨髓培养体系中加入当归注射液后ＣＦＵ－Ｅ、ＢＦＵ－Ｅ、ＣＦＵ－ＧＭ产率均明显提高，以终浓度为５０μｇ／ｍｌ时最佳，以此浓度加入再障患者骨髓常规培养体系中，后者的ＣＦＵ－Ｅ、ＢＦＵ－Ｅ、ＣＦＵ－ＧＭ产率分别提高３６．２％、     

IL—1影响L—细胞造血调节作用的实验研究

目的：研究白细胞介素－１与Ｌ－细胞在造血调控中的可能关系,丰富造血调控理论。方法：在体外祖细胞培养中以不同浓度的ＩＬ－１诱导和未经诱导的Ｌ－细胞条件培养液替代造血生长因子,观察集落产率的变化,并与ＩＬ－１和ＬｃＣＭ共同作用后的集落产率作比较。结果：ＩＬ－１在１２．５￣１００ｕ／ｍｌ浓度范围内,ＬｃＣＭＩＬ－１作者后的粒－单系祖细胞（ＣＦＵ－ＧＭ）,早期红系祖细胞（ＢＦＵ－Ｅ）,晚期红系祖细胞（ＣＦ     

姜黄素对小鼠CFU—GM及WEHI—3B细胞增殖的影响

用体外半固体集落培养方法，观察不同浓度姜黄素对ＢＡＬＢ／ｃ小鼠骨髓粒－巨噬系祖细胞及骨髓单核系白血病干细胞增殖的影响。结果显示：姜黄素呈剂量依赖性抑制ＣＦＵ－ＧＭ和ＷＥＨＩ－３Ｂ细胞增殖，其半抑制剂量分别为１．０３６＊１０＾－５ｍｏｌ．Ｌ＾－１和１．２２０＊１０＾－６ｍｏｌ．Ｌ＾－１。表明姜黄素对ＷＥＨＩ－３Ｂ白血病细胞的抑制作用强于对ＣＦＵ－ＧＭ的作用。     

肺泡巨噬细胞培养上清液对小鼠粒—单系造血祖细胞和造血干细胞的…

目的：观察肺泡巨噬细胞培养上清液（ＡＭψＳ）对粒－单系造血祖细胞（ＣＦＵ－ＧＭ）和造血干细胞的影响。方法：采用脾集落形成和造血祖细胞体外培养技术。结果：ＡＭψＳ对ＣＦＵ－ＧＭ的增殖具有双向调控作用，即低浓度有促进作用，高浓度有抑制作用，但它对ＣＦＵ－Ｓ无明显影响。结论：ＡＭψＳ中含有粒－单系集落刺激因子（ＣＳＦ－ＧＭ）和前列腺素样活性物质，这些物质浓度的变化可以调控ＣＦＵ－ＧＭ的增殖，大肠杆菌脂多     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.