Growth regulatory peptides in gastric mucosa.

1. Epidermal growth factor and the related peptide transforming growth factor alpha have been implicated in the stimulation of gastric mucosal proliferation. We assessed the immunohistochemical distribution of these peptides and their receptor, epidermal growth factor receptor, in mucosa from the antrum and body of the stomach from 28 patients. Twenty-three of the 56 biopsies were histologically normal (12 antrum and 11 body), whereas the other 33 showed varying degrees of inflammation. 2. Epidermal growth factor, transforming growth factor alpha and epidermal growth factor receptor had maximal density of distribution on the apical surfaces of the superficial epithelial cells, but were also expressed to a lesser extent on neck and body cells of the glandular tissue (P less than 0.05). We also demonstrated that epidermal growth factor expression was greater in the epithelial cells of inflamed mucosa than in those of normal mucosa (P less than 0.05). 3. We assessed mucosal proliferation by the Ki-67 labelling index. Ki-67-positive cells were found predominantly in the neck area of the glands and were more frequent in glandular antral tissue than in body glandular tissue (P less than 0.05). Expression of epidermal growth factor receptor in the neck and isthmus cells had a significant correlation with the Ki-67 labelling index (P less than 0.05). 4. We conclude that epidermal growth factor and epidermal growth factor receptor may be important in the adaptation of gastric mucosa to inflammation.     

肠上皮化生胃黏膜中多基因表达的同步检测

目的 研究肠上皮化生胃黏膜组织中p21^ras、表皮生长因子受体（EGFR）和p53突变蛋白的表达状况及其意义。方法 应用免疫组织化学方法对44例肠上皮化生组织、10例正常胃黏膜和31例胃癌组织中p21^ras、EGFR和p53突变蛋白的表达进行同步检测。结果 44例肠上皮化生组织中，p21^ras、EGFR和p53突变蛋白的表达阳性率分别为59．1％、54．6％和20．5％；31例胃癌组织中，p21^ras、EGFR和p53突变蛋白的表达阳性率分别为64．5％、54．8％和32．3％；肠上皮化生组织和胃癌组织间3种基因蛋白的表达阳性率差异无显著性（P〉0．05）。结论 肠上皮化生胃黏膜组织中存在多种基因的表达异常，值得深入研究。     

Ki-67在乳腺浸润性导管癌中的表达及其临床意义

目的：探讨Ki-67在乳腺浸润性导管癌中的表达及其与乳腺癌各临床病理指标的相关性。方法采用免疫组织化学方法检测362例乳腺浸润性导管癌患者肿瘤组织中Ki-67的表达,并分析Ki-67的表达与患者各项临床病理指标的相关性。结果 Ki-67阳性比例为59.7％（216/362）。Ki-67的表达与肿瘤大小成正相关（P＜0.05）,与淋巴结转移、组织细胞学分级、脉管癌栓、人表皮生长因子受体-2、表皮生长因子受体、拓扑异构酶Ⅱ-α表达成正相关（均为P＜0.01）,与雌激素受体、孕激素受体表达成负相关（均为P＜0.01）,与P53蛋白、TNM分期无明显相关性。结论 Ki-67的表达与大部分乳腺浸润性导管癌的临床病理指标有相关性,提示其可以作为监测此类患者预后的指标。     

乳腺导管内癌微浸润癌分子生物标志物的表达及其与超声表现的相关性

目的 :探讨乳腺导管内癌(ductal carcinoma in situ,DCIS)与导管内癌伴微浸润(ductal carcinoma in situ with microinvasion,DCIS-Mi)间的分子生物学表达差异,并分析其与超声征象间的相关性。方法:回顾性分析199例DCIS患者的术前完整超声影像资料及可供分析的雌激素受体、孕激素受体、人表皮生长因子受体2(human epidermal growth factor receptor 2,HER2)、Ki-67指数等免疫组织化学指标,比较2组间分子生物学表达的差异,并探讨其与患者超声表现间的相关性。结果:199例患者中,DCIS为123例(61.8%),DCIS-Mi为76例(38.2%)。DCIS组与DCIS-Mi组间的雌激素受体、孕激素受体表达无统计学差异(P>0.05),而DCIS-Mi组的HER2阳性率及Ki-67指数均高于DCIS组(P<0.05)。2组病灶的各超声征象中,微钙化的存在与HER2阳性表达相关,而肿块的最大径及Adler血供分级与Ki-67指数高表达相关(P<0.05)。结论:DCIS-Mi患者较DCIS患者在分子生物学表达方面预后更差,而部分超声征象与DCIS及DCIS-Mi预后差的分子生物学表达间存在相关性。     

In situ distribution of oncogene products and growth factor receptors in breast carcinoma: c-erbB-2 oncoprotein, EGFr, and PDGFr-beta-subunit

An increasing body of evidence suggests that breast tumour growth is mediated by oncogene products and growth factors which are or which act through cell surface receptors. The aims of the present study were to determine how three of these receptors, c-erbB-2 protein, epidermal growth factor receptor (EGFr) and the beta-subunit of platelet-derived growth factor receptor (PDGFr-beta-subunit), can effectively be demonstrated by immunohistochemical methods in breast tumors, how these receptors are distributed at the cellular level and how their expression correlates with well-established prognostic indicators including hormone receptors and proliferative index. We examined frozen tissue sections of 50 invasive human breast carcinomas, including 45 ductal, four lobular, and one mucinous tumours, by immunocytochemical methods to determine the in situ distributions of c-erbB-2, EGFr, and PDGFr-beta-subunit. We compared staining for c-erbB-2 protein in frozen sections with that in paraffin sections of the same 50 tumours. The immunohistochemical labelling results were compared with tissue hormone receptor content and growth fraction determined by Ki-67 labelling. Strong labelling of tumour cells in frozen sections was detected in 22% of cases, all of the ductal type, stained with rabbit antiserum to c-erbB-2. Labelling for c-erbB-2 protein was generally weaker in paraffin sections than in frozen sections and in six of 11 positive cases, specific staining could be detected only in frozen sections. In immunostains with monoclonal antibody to EGFr, rare cells within tumour were labelled in 60% of the carcinomas. Using a monoclonal antibody to the beta-subunit of PDGFr, consistent labelling of fibrillary cellular processes in the walls of blood vessels and in fibrous stroma around tumour cell nests was detected, but there was no labelling of tumour cells themselves. C-erbB-2 oncoprotein positive tumours were found to be more often oestrogen receptor negative (P less than 0.005) or oestrogen and progesterone receptor negative (P less than 0.01) than c-erbB-2 negative tumours. No significant correlation was observed between c-erbB-2 expression and Ki-67 growth fraction.     

不同分子分型乳腺浸润性导管癌中的P53、表皮生长因子受体及Ki-67的表达及意义

目的探讨不同分子分型乳腺浸润性导管癌手术病例标本中P53、表皮生长因子受体(EGFR)和Ki-67的表达及临床意义。方法采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶连接法法对2010年1月-2011年7月446例乳腺浸润性导管癌患者标本进行分子分型,并同时检测其P53、EGFR、Ki-67等的表达。结果 P53和Ki-67在人类表皮生长因子受体2(HER2)过表达型、基底细胞样型、未分类型中的表达明显强于管腔A型及管腔B型(P<0.05);HER2过表达型和未分类型中的EGFR表达明显强于管腔A型及管腔B型(P<0.05)。结论在使用雌激素受体、c-erbB-2等指标对浸润性导管癌进行分子分型时同时检测P53、EGFR及Ki-67等标记物,有助于更加精准的评估肿瘤的生物学行为及预后,对靶向药物的个体化治疗提供参考和疗效预测有重要意义。     

Localization of transforming growth factor alpha and its receptor in gastric mucosal cells. Implications for a regulatory role in acid secretion and mucosal renewal.

Transforming growth factor alpha (TGF alpha) shares with epidermal growth factor (EGF) structural homology (35%), a common cell-surface membrane receptor (TGF alpha/EGF receptor), and a nearly identical spectrum of biological activity, including inhibition of gastric acid secretion. Herein, we report expression of TGF alpha mRNA in normal gastric mucosa of the adult guinea pig, rat, and dog. TGF alpha mRNA was also detected in matched surgically resected gastric mucosa and adjacent gastric carcinoma from 10 patients, and in gastric mucosa adjacent to a benign ulcer from an additional patient. TGF alpha protein was quantitated by radioimmunoassay and was present in tumor and adjacent mucosa. TGF alpha/EGF receptor mRNA was also detected in gastric mucosa from all species studied. Localization of TGF alpha and TGF alpha/EGF receptor mRNA expression was examined in samples of unfractionated guinea pig gastric mucosa and from chief cell-enriched and parietal cell-enriched fractions. All samples exhibited TGF alpha and TGF alpha/EGF receptor expression. The TGF alpha signal was greatest in the parietal cell fraction (5.8-fold increase), but was also enhanced in the chief cell fraction (1.9-fold increase) relative to the unfractionated gastric mucosa. Like TGF alpha expression, TGF alpha/EGF receptor mRNA expression was most intense in the parietal cell-enriched fraction (7.8-fold increase), but was also increased in the chief cell-enriched fraction (2.7-fold increase) relative to the unfractionated guinea pig gastric mucosa. We conclude that TGF alpha and TGF alpha/EGF receptor genes are expressed in normal adult mammalian gastric mucosa. These findings, when interpreted in light of described actions of TGF alpha and EGF, provide evidence that local production of TGF alpha could play an important role in the regulation of acid secretion and mucosal renewal in the stomach.     

胃黏膜腺体上皮反应性增生与异型增生的判断及临床意义

目的探讨胃黏膜腺体上皮反应性增生（gastric epithelial reactive hyperplasia,GERH）及胃黏膜腺体上皮轻度异型增生（gastric epithelial low—gradedysplasia,GELD）的临床病理特征以及P53、增殖细胞核抗原（Ki-67）对于判别两种性质增生的临床意义。方法将我院2010年1月-2011年12月胃黏膜活检组织中伴腺体上皮轻度非典型增生139例,根据Padova及Vienna标准分为GERH组（106例）及GELD组（33例）,观察两组病变的组织形态学特点,并分忻P53、Ki-67在两组的表达情况。结果GERH组多伴有明显的炎症背景,腺体增生向黏膜表面或固有膜逐渐分化成熟,无明显的界限;GELD组缺乏炎症背景,且GELD的腺体上皮可延伸至黏膜上皮表面或向下面固有膜及其周围形成界限清楚的局灶状分布。P53在GERH组及GELD组中阳性表达率分别为2．83％及15．15％,两组比较差异有统计学意义（P〈0．05）。GERH组中Ki-67阳性表达率24．53％,Ki-67表达位于小凹基底部（下1／3）95例（89．62％）;GELD组中Ki-67阳性表达率90．90％,表达位于小凹基底部及侧面（达中1／3）13例（39．39％）,两组在表达数量及分布部位方面比较差异有统计学意义（x2=46．10,P〈0．005;x2=14．83,P〈0．005）。结论临床诊断胃黏膜腺体上皮GERH及GELD时,结合组织形态学变化及P53、Ki-67的表达情况可为两者鉴别提供重要依据。     

胃腺癌组织P13Kp85α、pAkt及Ki-67表达和意义

目的利用组织芯片检测P13Kp85α、pAkt、Ki-67蛋白在胃黏膜、胃腺癌及癌旁组织的表达情况,探讨其意义。方法用免疫组化技术检测了30例胃黏膜组织、85胃腺癌组织（分为高、中、低分化3种）及其癌旁组织中P13Kp85α、pAkt及Ki-67的表达。结果P13Kp85α、pAkt和Ki-67蛋自在胃黏膜、癌旁组织及胃腺癌组织中的阳性表达率差异有统计学意义（χ^2=41．03～57．58,P〈0．01）,随病情进展三者表达逐步增强（Hc=6．86～13．30,P〈0．05、0．01）。在不同分化程度的胃腺癌中P13Kp85α、pAkt和Ki-67蛋白的阳性表达率差异有显著性（χ^2=8．08～18．90,P〈0．01）,随肿瘤恶性程度增高其阳性表达增强（Hc=15．95～20．10,P〈0．01）。P13Kp85α、pAkt与Ki-67蛋白三者的表达两两相关（r=0．82、0．81、0．86,P〈0．01）。结论P13Kp85α、pAkt和Ki-67的表达与胃腺癌的发生密切相关。     

Regulation by endogenous interleukin-1 of mRNA expression of healing-related factors in gastric ulcers in rats.

We investigated the role of endogenous interleukin (IL)-1 in the mRNA expression of cyclooxygenase (COX)-1, COX-2, inducible nitric oxide synthase (iNOS), cytokine-induced neutrophil chemoattractant (CINC)-1, epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), hepatocyte growth factor (HGF), and transforming growth factor (TGF)-beta1 in acetic acid-induced gastric ulcers in rats. IL-1beta mRNA was not detected in the normal or intact mucosa of ulcerated stomachs, but its expression was induced in the ulcerated tissue. IL-1beta immunoreactivity was observed in macrophages/monocytes and fibroblasts in the ulcer base. COX-2, iNOS, and CINC-1 mRNAs were expressed by ulceration. EGF, bFGF, HGF, and TGF-beta1 mRNA expression was detected in the normal mucosa, and their levels were significantly elevated by ulceration. In contrast, COX-1 mRNA level did not differ between the normal and ulcerated tissues. In a culture of isolated ulcer bases, block of IL-1 with IL-1 receptor antagonist (IL-1RA) dose-dependently and significantly reduced the mRNA levels of COX-2, iNOS, CINC-1, HGF, and bFGF. In contrast, COX-1, EGF, and TGF-beta1 mRNA expression was not affected by IL-1RA. IL-1RA dose-dependently reduced prostaglandin E(2) production, total and iNOS activities, neutrophil chemotactic activity, and growth-promoting activity toward gastric epithelial cells in the ulcer base. Finally, the administration of IL-1RA caused a significant impairment of ulcer healing. These results indicate that IL-1, expressed in macrophages/monocytes and fibroblasts in the ulcer base, might up-regulate the mRNA expression of COX-2, iNOS, CINC-1, HGF, and bFGF, thereby contributing to gastric ulcer healing in rats.     

胃癌组织中胰岛素样生长因子结合蛋白-2的表达及其临床意义

目的 探讨胃癌组织中胰岛素样生长因子结合蛋白-2（IGFBP-2）的表达及与细胞增殖的关系。方法 采用免疫组化EnVision法检测97例胃癌组织和30例正常胃黏膜中的IGFBP-2表达，同时用Ki-67标记细胞增殖。结果 胃癌组织中IGFBP-2的表达明显高于正常组织（P〈0．05）。IGFBP-2在高级别癌中表达高于低级别癌，但差异不显著（P〉0．05）。中晚期胃癌中IGFBP-2的表达明显高于早期胃癌，有淋巴结转移者高于无淋巴结转移者，随着肿瘤临床分期增高，IGFBP-2的表达明显升高，差异显著（P〈0．05）。胃癌组织中IGFBP-2的表达与Ki-67呈正相关（P〈0．05）。结论 IGFBP-2可能通过促进细胞增殖参与了胃癌的发生、发展。     

乳腺浸润性导管癌超声特征与ER、PR、CerB-2、Ki-67表达的相关性

目的探讨乳腺浸润性导管癌（IDC）超声特征与雌激素受体（ER）、孕激素受体（PR）、人类上皮生长因子受体2（CerB-2）、增殖细胞核抗原（Ki-67）表达的相关性。 方法收集2015年6月至2019年2月我院收治的189例IDC患者的临床资料，对超声资料及分子病理学指标进行分析。 结果ER、PR、CerB-2、Ki-67蛋白在IDC中的阳性表达率分别为63.4%（120/189）、52.9%（100/189）、45.5%（86/189）、65.6%（124/189）。IDC超声表现为肿块边缘有毛刺征患者的ER、PR阳性率高于无毛刺征患者，差异有统计学意义（70.0% vs. 56.2%，60.0% vs. 44.9%，P<0.05），有微钙化患者的CerB-2、Ki-67阳性率高于无钙化患者，差异有统计学意义（53.4% vs. 38.8%，73.2% vs. 59.2%，P<0.05），血流信号丰富患者的CerB-2、Ki-67阳性率高于乏血供患者，差异有统计学意义（52.2% vs. 35.8%，72.1% vs. 56.4%，P<0.05），有腋窝可疑淋巴结患者的CerB-2阳性率高于无可疑淋巴结患者，差异有统计学意义（54.3% vs. 34.8%，P<0.05）。 结论IDC的超声特征与ER、PR、CerB-2、Ki-67的表达有相关性，超声检查可为乳腺癌的术前诊断、治疗及评估预后提供重要依据。     

良恶性胃黏膜中p21ras、表皮生长因子受体和p53蛋白的表达及意义

目的研究p21^ras、表皮生长因子受体（EGFR）和p53突变蛋白在良恶性胃黏膜组织中的表达及其对胃癌早期诊断的意义。方法应用免疫组织化学方法对31例胃癌、49例癌前病变、21例萎缩性胃炎和28例正常胃黏膜组织中的p21^ras、EGFR和p53突变蛋白的表达进行检测。结果31例胃癌组中,p21^ras、EGFR和p53突变蛋白的阳性表达率分别为64．5％、54．8％和32．3％;49例癌前病变组中,p21^ras、EGFR和p53突变蛋白的阳性表达率分别为59．2％、53．1％和22．4％;癌前病变组和胃癌组间3种基因蛋白的阳性表达率差异均无显著性（均为P〉0．05）,但均显著高于正常胃黏膜组（P〈0．05或P〈0．01）。结论胃癌和癌前病变胃黏膜组织中存在多种基因的表达异常,检测胃黏膜中p21^ras、EGFR和p53蛋白对胃癌早期诊断的意义不大。     

IVIM多参数预测乳腺癌免疫组化指标表达的可行性研究

目的基于扩散加权成像(diffusion weighted imaging,DWI)和体素内不相干运动扩散加权成像(intravoxel incoherent motion diffusion-weighted imaging,IVIM-DWI)技术,探究乳腺癌表观扩散系数(apparent diffusion coefficient,ADC)值和IVIM-DWI多参数值(D值、D*值、f值)与乳腺癌免疫组化指标(ER、PR、HER-2和Ki-67)不同表达情况之间是否具有统计学差异。材料与方法前瞻性搜集怀疑为乳腺癌的患者,术前进行MRI评估,并进行IVIM-DWI成像,利用单指数DWI模型测量ADC值,利用双指数IVIM模型对多b值DWI进行后处理,通过后处理工作站获得IVIM各参数值:D值、D*值及f值。追踪所有手术病人病理结果,最终入组经病理确诊的乳腺癌患者共51例。采用SPSS 19.0统计软件进行数据分析,探究乳腺癌ADC值和IVIM-DWI多参数值(D值、D*值、f值)与乳腺癌免疫组化指标(ER、PR、HER-2和Ki-67)不同表达情况之间是否具有统计学差异,采用独立样本t检验或U检验。结果 51例乳腺癌患者共55个病灶。其中ER阳性、PR阳性、HER-2阳性及Ki-67高表达分别为45个、40个、14个、36个病灶。经统计学分析发现以Ki-67表达百分比为14%为阈值,Ki-67高表达组与低表达组的D平均值差异有统计学意义(P=0.046),D*和f平均值差异无统计学意义;55个乳腺癌病灶中,ER、PR、HER-2阴性组和阳性组的D、D*及f平均值差异均无统计学意义(P0.05);ER、PR、HER-2阳性组与阴性组及Ki-67高表达组与低表达组的ADC平均值差异均无统计学意义(P0.05)。结论乳腺癌Ki-67高低表达组的IVIM-DWI定量参数D值存在统计学差异,提示了通过非侵入性的影像学检查方法预测Ki-67表达情况的潜能。     

胃腺癌组织Survivin和Ki-67表达及其意义

目的探讨Survivin与Ki-67在胃腺癌组织表达及其临床意义。方法应用免疫组化方法检测20例正常胃黏膜组织和50例胃腺癌组织Survivin和Ki-67表达情况。结果胃腺癌组织组织Survivin与Ki-67阳性表达率均明显高于正常胃黏膜组织（Uc=3.693、5.640,P〈0.001）;Survivin阳性表达与病人性别、年龄、组织分化程度、TNM分期及浸润深度均无关（Uc=1.299、0.679,Hc=0.488、0.369、0.756,P〉0.05）,而与淋巴结转移有关（Uc=2.138,P〈0.05）;Ki-67阳性表达与TNM分期及淋巴结转移有关（Hc=6.539,Uc=2.987,P〈0.05、0.01）,而与病人性别、年龄、组织分化程度和浸润深度均无关（Uc=0.645、0.976,Hc=4.070、3.243,P〉0.05）。胃腺癌组织Survivin的表达与Ki-67呈正相关（rs=0.321,P〈0.05）。结论Survivin蛋白可能具有促进肿瘤细胞增殖活性的作用,联合检测Survivin和Ki-67对判断淋巴结转移的危险性具有较高的价值。     

表皮生长因子受体在早期胃癌中的表达

本文采用ＡＢＣ免疫组化方法，检测了３９例早期胃癌中表皮生长因子受体的分布。发现表皮生长因子受体在正常胃粘膜中几乎不存在，而在早期胃癌和癌旁不典型增生胃粘膜中的阳性百分比（５１．２８％和４８．７２％）没有显著性差异，在印戒细胞癌和低分化腺癌中，表皮生长因子受体的阳性百分比显著高于高分化腺癌和中分化腺癌。提示表皮生长因子受体的过度表达与早期胃癌的生物学行为有一定正相关。     

细针吸取细胞块免疫细胞化学检测在乳腺癌转移灶的应用研究

目的 用乳腺癌转移灶细针吸取细胞学细胞蜡块,检测雌激素受体(ER)、孕激素受体(PR)、人表皮生长因子受体2(HER2)及Ki-67蛋白表达,探讨与原发灶肿瘤细胞表达的差异.方法 收集中国医学科学院肿瘤医院108例乳腺癌患者转移灶细胞蜡块标本,全自动免疫组化仪检测ER、PR、HER-2及Ki-67的蛋白表达.结果 ER...     

EGFR、VEGF及Ki-67在肺浸润性腺癌和微浸润性腺癌及浸润前病变组织中的表达及意义

目的：探讨EGFR、VEGF及Ki-67在肺浸润性腺癌、微浸润性腺癌及浸润前病变组织中的表达,及其与临床病理特征之间的相关性。方法按照2011年IASLC/ATS/ERS多学科肺腺癌新分类方案,对2004年1月至2010年12月间日照市人民医院常规病理诊断中确诊为肺腺癌、细支气管肺泡癌和不典型腺瘤样增生的病例,使用和整合病理组织学、免疫组化TTF-1和P63[或（和）CK5/6]及临床、影像学多学科知识,重新诊断、分类,应用免疫组织化学S-P法检测128例浸润性肺腺癌、28例微浸润性腺癌、46例浸润前病变（29例原位腺癌AIS、17例不典型腺瘤样增生）病理组织中EGFR、VEGF及Ki-67蛋白的表达,以30例癌旁组织、30例正常肺脏组织作对照,并结合临床病理特征进行相关性分析,比较EGFR、VEGF及 Ki-67：（1）在浸润性腺癌、微浸润性腺癌及浸润前病变三种不同组织中表达差异,及其与正常黏膜组织差异;（2）三者在28例微浸润性腺癌中微浸润灶与原位腺癌两种成分中表达的差异;（3）三者的表达与肿瘤大小、分化程度、浸润程度、淋巴结转移等临床病理因素的关系;（4）EGFR、VEGF及Ki-67三者在浸润性腺癌中表达的相关性。结果（1）EGFR、VEGF及Ki-67的表达在浸润前病变、微浸润性腺癌及浸润性肺腺癌组织中的表达依次增高,与正常对照组比较,差异有统计学意义（P＜0.01）。（2）EGFR、VEGF及Ki-67在微浸润性腺癌微浸润与原位两种成分中表达的差异无统计学意义（P＞0.05）;与浸润性腺癌比较,Ki-67表达差异有统计学意义（P＜0.05）,EGFR、VEGF的表达差异无统计学意义（P＞0.05）。（3）肿瘤大小≤2 cm组与＞2 cm且＜3 cm组间比较,EGFR、VEGF及Ki-67表达差异有统计学意义（P＜0.05）;肿瘤大小＞2 cm且＜3 cm组与≥3 cm组比较,VEGF表达差异有统计学意义（P＜0.05）,EGFR及Ki-67表达无明显差异（P＞0.05）。（4）EGFR、VEGF及Ki-67表达与组织学类型（鳞屑样、腺泡样、乳头状、实性）无关（P＞0.05）;EGFR 高表达与组织学分级低分化、淋巴结转移及肿瘤高分期有关（P＜0.05）;VEGF与组织学分级、淋巴结转移及肿瘤高分期均无关（P＞0.05）;Ki-67只与淋巴结转移有关（P＜0.05）,而与组织学分级、肿瘤高分期无关（P＞0.05）。EGFR、VEGF 及 Ki-67三者表达正相关（P＜0.05）。结论本组显示原位腺癌大小以2 cm为界更能较好地显示生物学行为;EGFR、VEGF及Ki-67高表达与肺腺癌发生、浸润、转移密切相关,早期联合检测能辅助预测肺腺癌的生物学行为、评估预后和指导治疗。     

胃泌素、环氧合酶2在不同胃黏膜病变中的表达及意义

目的 研究不同胃黏膜病变组织中胃泌素、环氧合酶2(COX-2)和Ki-67的表达,探讨胃泌素和COX-2在胃黏膜癌变过程中的作用及其相互关系.方法 96例不同胃黏膜病变组织来自胃镜活检标本.采用免疫组化染色EnVision法检测胃泌素、COX-2和Ki-67表达.结果 胃泌素在慢性浅表性胃炎(CSG)、慢性萎缩性胃炎(CAG)、肠上皮化生(IM)、异型增生(Dy)和胃癌(GC)中表达的阳性率分别为58.8%、30.0%、57.9%、68.2%和77.8%,CAG组与GC组比较,差异有统计学意义(P<0.05).COX-2在CSG、CAG、IM、Dy和GC中表达的阳性率分别为41.2%、45.0%、68.4%、72.7%和88.9%;在GC中的表达显著高于CSG(P<0.05).从CSG→GC,Ki-67增殖指数(PI)呈逐渐递增趋势;从CAD→GC,胃泌素和COX-2的表达与PI呈正相关(P<0.05).在胃癌前期病变中,胃泌素与COX-2表达有显著相关性(γ=0.4108,P<0.01),二者共同表达时,PI显著升高.结论 CAG中胃泌素王低表达,从CAG→GC,胃泌素表达逐渐升高.在胃黏膜癌变过程中,COX-2表达呈逐渐递增趋势.在胃癌前病变阶段,胃泌素和COX-2协同促进细胞增殖,胃泌素可能参与上调COX-2的表达.