胰岛素促进体外培养的小鼠卵巢颗粒细胞增殖

目的：研究胰岛素对体外培养的小鼠卵巢颗粒细胞增殖的影响,探讨胰岛素的促增殖作用是否与浓度相关。方法：未成熟小鼠卵巢颗粒细胞原代培养,四甲基偶氮唑蓝法（MTT）测定颗粒细胞增殖指数,观察不同浓度胰岛素对颗粒细胞增殖的影响。结果：与对照组相比,5ng·ml^-1。胰岛素对颗粒细胞没有促增殖作用（P〉0.05）,10ng·ml^-1有促增殖作用（P〈0.05）,100ng·ml^-1、1000ng·ml^-1的促增殖作用更强（P〈0.01）,100ng·ml^-1、1000ng·ml^-1作用无明显差异（P〉0.05）。结论：生理浓度的胰岛素能促进颗粒细胞增殖,高浓度胰岛素促增殖作用更强,本实验为进一步研究高胰岛素血症在多囊卵巢综合症（PCOS）的卵泡发育停滞机制奠定基础。     

FSH和GDF-9对大鼠颗粒细胞蛋白激酶B表达的影响

目的探讨FSH和GDF-9对大鼠颗粒细胞中蛋白激酶B（PBK,又称Akt）的影响。方法应用Western blot法检测体外培养大鼠颗粒细胞中磷酸化Akt和总Akt的表达情况。结果FSH能15min内促进磷酸化Akt在颗粒细胞中的表达,而GDF-9则在4h后可以引起Akt的磷酸化。结论相对于GDF-9,FSH能在短时间内促进P—Akt的表达完成,Akt信号通路可能在FSH和GDF-9促进颗粒细胞生长中起一定的作用。     

抗苗勒氏管激素对人黄素化颗粒细胞激素分泌的影响

目的：探讨抗苗勒氏管激素（AMH）对人颗粒细胞激素产生分时段的影响。方法：采用人黄素化颗粒细胞原代培养，分组加入不同浓度的AMH，分时段测定细胞培养液中的雌二醇（E2）和孕酮（P）浓度。结果：细胞培养液中E2、P浓度均随培养时间延长而升高，升高幅度渐下降。随着AMH浓度的增加，颗粒细胞的E2和P分泌明显降低， AMH 5μg/L组到20 μg/L组间有剂量依赖性，AMH 50 μg/L组与20 μg/L组比较无显著差别。结论：AMH可以降低体外培养人黄素化颗粒细胞的雌、孕激素分泌，并有一定的剂量依赖性，提示AMH可以影响颗粒细胞的激素合成过程。     

两种SD大鼠多囊卵巢综合征模型的比较研究

目的比较两种SD大鼠多囊卵巢综合征动物模型的建模效果。方法建立两种PCOS动物模型。观察各组大鼠卵巢形态学、性激素及空腹血糖和胰岛素的变化。结果DHEA建模组大鼠的体重显著低于对照组（P〈0．05）;hCG建模组大鼠卵巢重量和体积均显著高于对照组（P〈0．05）。两建模组大鼠卵巢卵泡多呈囊性扩张,颗粒细胞层数减少,卵泡膜细胞、间质细胞增生。两建模组大鼠血清T、FINS、FBG和HOMA指数均显著高于对照组（P〈0．05）;DHEA建模组LH水平较对照组无显著性差异;hCG建模组大鼠血清LH、LH／FSH比值均显著高于对照组（P〈0．05）。DHEA建模组FSH水平显著高于对照组（P〈0．05）。结论利用hCG诱导SD幼年雌性大鼠出现了与PCOS患者极为相似的卵巢病理改变及LH、雄激素水平升高和胰岛素抵抗等典型内分泌变化,其建模效果优于DHEA。     

子宫切除对卵巢功能影响的临床研究

目的探讨子宫切除不同术式对卵巢功能的影响。方法对43例月经周期规则、卵巢正常、手术方式不同的子宫肌瘤患者,采用放射免疫法测定术前及术后性激素（FSH、E2）水平,以及术后是否出现卵巢功能衰退。结果术后2周子宫全切组及次全切组患者E2下降,与术前相比差异无显著性（P〉0.05）,FSH升高,与术前相比差异有显著性（P〈0.05）;术后3-12个月E2、FSH与术前相比无显著性差异（P〉0.05）;手术前后全切组与次全切组相比,E2、FSH变化均无显著性差异。结论子宫切除会引起卵巢功能暂时性下降,可于术后逐渐恢复,与手术方式无明显关系。     

PCOS患者血浆leptin和血清T、E2、FSH、LH、PRL检测的临床意义

目的：探讨多囊卵巢综合征（PCOS）患者血浆leptin和血清T、E2、FSH、LH、PRL水平的变化及临床意义。方法：应用放射免疫分析和发光法对31例PCOS患者进行了血浆leptin和血清T、E2、FSH、LH、PRL测定,并以正常妇女作对照。结果：PCOS患者血浆leptin和血清T、LH、PRL水平非常显著地高于正常妇女组（P〈0.01）,FSH、E2水平与正常妇女组比较无显著性差异（P〉0.05）,且血浆leptin水平与血清T、LH、PRL水平呈正相关（r=0.5784、0.5411、0.6082,P〈0.01）。结论：检测PCOS患者血浆leptin和血清T、E2、FSH、LH、PRL水平的变化与疾病的发生和发展密切相关。     

PCOS患者卵巢超声形态学改变与内分泌激素相关性的初步研究

目的探讨多囊卵巢综合征（PCOS）患者卵巢的形态学改变与内分泌激素的相关性。方法选择94例PCOS患者作为研究组,按体质量指数（BMI）分为肥胖型PCOS组（OB-PCOS）和非肥胖型PCOS组（NOB-PCOS）;同期选择69例有正常排卵的输卵管性不孕患者作为对照组,经阴道超声分别测量其卵巢总面积（A）,同时检测其黄体生成素（LH）、卵泡刺激素（FSH）、睾酮（T）、雌二醇（E2）、人体催乳素（PRL）水平,并分析其与A值之间的相关性。结果 1.PCOS组卵巢总面积明显高于对照组（P〈0.001）,LH、LH/FSH以及T水平较对照组明显升高,差异有统计学意义（P〈0.001）,FSH水平明显降低,差异有统计学意义（P〈0.05）;2.PCOS患者肥胖型组T水平较非肥胖型组升高,差异无统计学意义（P〉0.05）;3.相关性分析显示,PCOS组A值与T值呈正相关,差异有统计学意义（P〈0.05）。结论 PCOS的发病过程中,卵巢总面积的改变受内分泌激素的影响。     

Cyclin G1在小鼠卵巢颗粒细胞的表达及调控

目的:探讨cyclinG1在小鼠卵巢颗粒细胞的表达以及促性腺激素对其表达的调控作用。方法:以免疫组化方法检测小鼠卵巢颗粒细胞中cyclinG1的表达情况;取性成熟小鼠的颗粒细胞进行体外培养,分别加入卵泡刺激素(FSH)和绒毛膜促性腺激素(hCG)作用12h后,收集细胞,提取细胞总RNA,采用半定量RTPCR检测cyclinG1表达水平的变化。结果:免疫组化结果显示cyclinG1在不同发育阶段卵泡的颗粒细胞和卵丘细胞中均有较强表达,而黄体细胞中表达较少。RTPCR结果显示,FSH与hCG分别作用于体外培养的颗粒细胞12h后,与对照组比较,hCG可使cyclinG1的表达水平明显降低(P<0.01),FSH对其表达水平影响不明显(P>0.05)。结论:小鼠卵巢颗粒细胞中存在cyclinG1表达;hCG可降低cyclinG1在颗粒细胞中的表达水平,提示cyclinG1在颗粒细胞增殖分化过程中可能发挥正调节作用。     

冷应激促进PCOS大鼠的HSP90表达及疾病进展

目的 探究冷应激促进PCOS大鼠的HSP90表达及疾病进展。方法 选取40只SPF级SD雌性大鼠,随机分为正常（N）组、模型（M）组、促肾上腺皮质激素（A）组、冷应激（C）组,每组10只,H-E染色法检测卵巢组织病理形态,ELISA法及放射免疫法检测大鼠血清中生殖激素水平,免疫组化法检测卵巢组织中HSP90的蛋白表达。结果 与N组比较,M组动情前期、卵巢指数、LH、E2、T、INS明显升高（P<0.05）,动情期、P、FSH、HSP90蛋白明显降低（P<0.05）,与M组比较,A组、C组动情前期、卵巢指数、LH、E2、T、INS显著升高（P<0.05）,动情期、P、FSH、HSP90蛋白降低明显（P<0.05）,且C组比A组变化显著（P<0.05）;N组卵巢及卵母细胞结构正常、形态良好,M组可见卵巢皮质呈多囊样改变,黄体数量明显减少,出现大量小窦卵泡及囊性卵泡,卵巢苍白,卵泡膜细胞层明显增大,A组、C组卵巢病理更为严重。结论 冷应激可通过激活HSP90改变PCOS大鼠的血清生殖激素水平,诱导PCOS发生发展。     

二甲双胍联合复方醋酸环丙孕酮治疗PCOS的诊治分析

目的探讨复方醋酸环丙孕酮、二甲双胍对存在雄激素过多、胰岛素拮抗的多囊卵巢综合征（Polycystic ovary syndrome,PCOS）患者促排卵治疗以及提高妊娠率的效果。方法选择存在雄激素过多、胰岛素拮抗的耐克罗米酚的PCOS患者50例,口服复方CPA、二甲双胍治疗,3个月后,未恢复正常月经者联合克罗米酚促排卵,治疗前或治疗后每隔3个月分别测量体重、卵巢体积、空腹胰岛素、服糖后2h胰岛素、空腹血糖、服糖后2h血糖、睾酮（T）、性激素结合蛋白（SHBG）、促卵泡生成素（FSH）、促黄体生成素（LH）、雌二醇（E2）。结果经3个月治疗,治疗前后相比,血糖水平无改变,体重指数有下降,但无显著差异（P〉0.05）;卵巢体积由（17.6±7.3）mm^3下降至（7.4±0.1）mm^3,差异有显著性（P〈0.01）;空腹胰岛素及服糖后胰岛素有明显下降（P〈0.01）,LH和LH/FSH有明显下降（P〈0.05）,SHBG显著上升（P〈0.01）,FSH、E2无改变。成熟卵泡发育44例（88%）,排卵34例（68%）,妊娠17例（34%）。结论复方醋酸环丙孕酮、二甲双胍能有效地降低雄激素、改善胰岛素拮抗,提高排卵率和妊娠成功率。     

环磷酰胺对BALB／C（H-2^d）雌性成年小鼠卵巢形态结构与功能的影响

目的探讨造血干细胞移植前环磷酰胺预处理对卵巢的损伤作用。方法将60只BALB／C（H-2d）小鼠随机分为A组（20只），不给予任何处理；B组（20只），经腹腔内注入生理盐水0．5ml；C组（20只），以环磷酰胺溶解于生理盐水后，按380mg／kg剂量一次性腹腔注射。C组分别于预处理后15d、（C1组）、30d（C2组）各取10只小鼠处死，A组（A1组、A2组）和B组（B1组、B2组）分别于第2、4个间情期各取10只小鼠处死，分别在光镜和电镜下观察卵巢组织形态学改变，计数始基卵泡、初级卵泡、窦状卵泡和排卵前卵泡。放免法检测血清E2、P、T、FSH、LH、PRL。结果C组小鼠于预处理后出现精神萎靡、体重下降、阴道上皮细胞失去周期性变化等表现。卵巢体积缩小，光镜下颗粒细胞变性、坏死，发育期卵泡数减少，闭锁卵泡增加（P〈0．01），但始基卵泡数正常。电镜下可见卵泡透明带完整，卵母细胞、卵丘细胞和颗粒细胞均受到不同程度损伤。C组E2、P显著低于空白组和对照组，FSH、LH水平则显著高于A组和B组，（P〈0．01）；T、PRL水平在各组之间无显著性差异，预处理30d小鼠较15d损伤程度减轻。结论造血干细胞移植前化学药物预处理可造成卵巢结构与功能的损伤，但这种损伤未影响始基卵泡，卵巢尚有一定储备功能，随距药物作用时间的延长其结构与功能还可在一定程度上得以恢复。     

Differential responses of granulosa cells from small and large follicles to follicle stimulating hormone (FSH) during the menstrual cycle and acyclicity: effects of tumour necrosis factor-alpha

This study determined effects of follicle stimulating hormone (FSH) alone and in combination with tumour necrosis factor (TNF), on granulosa cells from small (5-10 mm diameter) and large (>10-25 mm) follicles during follicular and luteal phases of the cycle and during periods of acyclicity. Granulosa cells were collected from ovaries of premenopausal women undergoing oophorectomy. The cells were cultured with human FSH (2 ng/ml) and testosterone (1 microM) in the presence or absence of human TNF-alpha (20 ng/ml). Media were removed at 48 and 96 h after culture and progesterone, oestradiol and cAMP in media were measured by radioimmunoassays. FSH stimulated the accumulation of oestradiol from granulosa cells of small follicles during the follicular and luteal phases but not during acyclicity; and TNF reduced oestradiol accumulation in the presence of FSH. Interestingly, in granulosa cells from small follicles, progesterone and cAMP secretion increased in response to FSH and neither was affected by TNF. Thus, TNF specifically inhibited the conversion of testosterone to oestradiol in granulosa cells from small follicles. FSH stimulated oestradiol production by granulosa cells of large follicles obtained only during the follicular phase of the cycle and TNF inhibited the FSH-induced oestradiol secretion. Granulosa cells obtained from large follicles during the luteal phase and during acyclicity did not accumulate oestradiol in response to FSH. However, FSH increased progesterone and cAMP secretion by granulosa cells obtained from large follicles during the follicular and luteal phases. During the luteal phase alone, TNF in combination with FSH increased progesterone accumulation above that of FSH alone. FSH did not increase progesterone, oestradiol or cAMP secretion by granulosa cells obtained from large follicles during acyclicity. Thus, FSH increases progesterone, oestradiol and cAMP secretion by granulosa cells of small follicles during the follicular and luteal phases and TNF appears to inhibit FSH-induced oestradiol secretion specifically in those cells. In large follicles, FSH- stimulated granulosa cell secretion of oestradiol is limited to the follicular phase and this effect can be inhibited by TNF. In addition, when granulosa cells of large follicles do not increase oestradiol secretion in response to FSH, TNF stimulates progesterone secretion.      

Tumour necrosis factor-alpha inhibits follicle stimulating hormone-induced granulosa cell oestradiol secretion in the human: dependence on size of follicle

This study investigated the effects of tumour necrosis factor-(TNF) on human granulosa cells taken from ovaries of 11 premenopausalwomen undergoing oophorec-tomy during the luteal phase of thecycle for reasons unrelated to ovarian pathology. Granulosacells from follicles ranging from 5–10 mm diameter (small)and from >10–25 mm (large) were subjected to culturefor 48 and 96 h. Granulosa cells were cultured with human FSH(1 ng/ ml), testosterone (1 µM) and human TNF (10 ng/ml),each alone, and in various combinations. In granulosa cellsof small follicles, FSH alone increased progesterone and cAMPaccumulation and the conversion of testosterone to oestradiol.In granulosa cells of large follicles, FSH increased progesteroneand cAMP accumulation but not the conversion of testosteroneto oestradiol. Only in granulosa cells of small follicles didTNF significantly inhibit FSH-induced conversion of testosteroneto oestradiol but it was not apparent until the second 48 hof culture and concomitantly TNF did not alter the ability ofFSH to stimulate progesterone and cAMP accumulation. In granulosacells of large follicles, TNF did not alter FSH-stimulated oestradiol,progesterone or cAMP accumulation. Interestingly, progesteroneaccumulation in the presence of TNF and FSH was significantlygreater in granulosa cells of large follicles than in granulosacells of small follicles. The results indicate that TNF suppressesFSH-induced oestradiol secretion in granulosa cells from smallfollicles and this modulatory effect of TNF appears to be independentof decreases in progesterone and cAMP. The potential physiologicalsignificance of these findings is that TNF may be a relevantcytokine in suppressing FSH-induced oestradiol secretion andfollicular growth during the luteal phase of the cycle.     

Increasing progesterone secretion in human granulosa-luteal cells induced by human follicular fluid

Increasing evidence suggests that local ovarian agents playa central role in the regulation of follicular maturation andcorpus luteum formation. In previous studies, we have shownthat porcine follicular fluid induces granulosa cell luteinizationin sow, human and rat. In the present study, the effect wasinvestigated of either human follicular fluid (FF) alone, humanfollicle-stimulating hormone (FSH) alone, or both upon progesteronesecretion of human granulosa-luteal cells. Granulosa-lutealcells were cultured in the presence of either FSH (5, 50 and250 ng/ml), lyophilized FF (50 and 250 µg/ml) or both.Secretion of progesterone increased from a minimum of 2.5-foldto a maximum of 23-fold in the presence of FSH alone and, significantlyless (2-fold) in the presence of FF alone, compared to cellscultured in medium alone. The co-administration of FSH and FFwas significantly more effective than either alone, while additionof both FSH (250 ng/ml) and FF (250 µg/ml) gave maximalprogesterone secretion. In granulosa-luteal cells pre-culturedwith both FSH and FF, subsequent exposure to human chorionicgonadotrophin (HCG) alone increased progesterone secretion 1.6-foldto 11-fold, compared to cells pre-cultured with FSH alone. Theeffect of FF from individual follicles was also studied. FFfrom follicles yielding mature cumulus—oocyte complexeswas 4.2-fold more effective, than FF obtained from folliclesyielding immature cumulus—oocyte complexes in enhancingthe FSH stimulation of progesterone secretion. A pre-cultureof granulosa-luteal cells in FSH plus FF from mature follicleswas 1.8-fold more effective in enhancing HCG-induced progesteronesecretion, compared to FF from immature follicles. This studysuggests that progesterone secretion by granulosa-luteal cellsis regulated by gonadotrophins and other factors accumulatingin FF.     

Endocrinology: Somatostatin action on rat ovarian steroidogenesis

To date, very few studies on the effect of somatostatin on femalereproductive function have been reported. In our study, we examinedthe effects of somatostatin on (i) androgen biosynthesis usingwhole ovarian dispersates, and (ii) aromatase activity and progesteroneproduction using granulosa cells. Whole ovarian dispersatesobtained from immature rats were cultured for 96 h in serum-freemedium with human chorionic gonadotrophin (HCG; 25 ng/ml) andinsulin (10 µg/ml) in the presence or absence of an increasingconcentration of somatostatin (0.03–3.00 ng/ml). HCG-and insulin-stimulated accumulation of androsterone by thesecells was inhibited significantly by somatostatin. Granulosacells from diethyl-stilbestrol-treated rats were cultured for48 h in serum-free medium with follicle-stimulating hormone(FSH; 20 ng/ml) and FSH plus insulin (1 µg/ml) with orwithout somatostatin (0.03–3.00 ng/ml). Both aromataseactivity and progesterone production stimulated by FSH and FSHplus insulin were significantly inhibited by somatostatin. Somatostatinby itself (1 ng/ml) did not have an effect on any of the evaluatedparameters. The action of somatostatin could be immunoneutralizedand did not influence the plated viable cell mass. These findingsindicate that somatostatin can regulate ovarian steroidogenesisby mediating gonadotrophin and growth factor action on differentovarian cell types.     

Smad2/Smad3蛋白在大鼠卵巢颗粒细胞中的表达及FSH对其活化的影响

目的 研究Smad2/Smad3蛋白在大鼠卵巢颗粒细胞中的表达及FSH对其活化的影响.方法 21d SD雌性大鼠,注射PMSG 20IU,48h后对卵巢颗粒细胞进行原代培养,用TGFβRⅡ抗体及不同浓度的FSH对细胞进行不同时间的处理,通过免疫细胞化学方法观察TGFβ信号通路中Smad2/Smad3和P-Smad2/P-Smad3(磷酸化Smad2/3)表达的变化.结果 1.Smad2/Smad3主要定位于卵巢颗粒细胞胞质,其活化形式P-Smad2/P-Smad3有少量表达;2.经FSH处理后, Smad2/Smad3向核内转移增多,P-Smad2/P-Smad3的表达增强,并与FSH的作用时间及剂量呈正相关性;3.TGFβRⅡ被抗体完全中和后再用FSH处理,Smad2/Smad3的核阳性率无显著增多, P-Smad2/P-Smad3的表达未见明显增强.结论 1.FSH促进Smad2/Smad3的磷酸化;2.FSH对 Smad2/Smad3的激活作用与TGFβ受体密切相关.     

Cultured human granulosa cells secrete a follicle stimulating hormone receptor-binding inhibitor

We previously reported that human follicular fluid contained a protein that inhibits binding of 125I-human FSH to its membrane receptor (FSH- BI) and demonstrates FSH-like agonist activity in vitro. The cellular origin of FSH-BI was unknown, although ovarian granulosa cells seemed a likely source. To address this question, human granulosa cells were collected from patients during routine in-vitro fertilization (IVF) or gamete intra-Fallopian transfer (GIFT) procedures. Cells from 98 patients were cultured and then examined for their ability to secrete FSH receptor-binding inhibitory activity into the culture medium. The function of the cultured cells was confirmed by their ability to respond to added FSH with conversion of exogenous androstenedione to oestradiol. Radioreceptor assays were performed individually on cell culture medium obtained from granulosa cell cultures from these 98 patients. Cultured granulosa cells, under basal conditions (in the absence of FSH stimulation), secreted significant FSH-BI activity into the culture medium. In order to accumulate enough material for further study, this culture medium was pooled and lyophilized. The lyophilized medium retained FSH-BI activity, and also demonstrated FSH agonist activity by stimulating oestradiol synthesis in cultured rat Sertoli cells. A fraction showing a single component after purification by polyacrylamide gel electrophoresis had an estimated molecular weight of 25000, and inhibited 125I-human FSH binding to receptor by 50% at 2.5 microg/ml. The results indicate that human granulosa cells secrete a protein with FSH-like activity having potential significance as a local regulator of FSH action in the ovary.      

The effects of insulin, and insulin-like growth factors I and II on human ovarian follicles in long-term culture

The aim of this study was to investigate the effects of insulin and insulin-like growth factors I and II (IGF-I and IGF-II) on human ovarian follicles in vitro. Ovarian cortical tissue slices (0.1-0.3 cm) were cultured for 7 or 14 days on an artificial extracellular matrix and with FSH. The ovarian tissue cultures were stimulated by insulin (33 ng/ml), IGF-I (20 or 50 ng/ml) or IGF-II (20 ng/ml). Combined effects of IGF-I (20 ng/ml) or IGF-II (20 ng/ml) and insulin (33 ng/ml) were also studied. Proliferating cell nuclear antigen (PCNA) was selected for immunohistochemical examination activation of the mitotic cell cycle in granulosa cells. After 1 week of culture the number of follicles had decreased in all cases. After 2 weeks of culture the number of healthy follicles had decreased dramatically in control cultures. However, the loss of follicles could be prevented with insulin and IGFs. The number of atretic follicles was significantly lower in insulin cultures compared with control cultures after 2 weeks. The proportion of primary follicles was significantly increased in cultures treated with insulin, IGF-I (50 ng/ml) or IGF-II (20 ng/ml) compared with control cultures after 2 weeks. A similar effect was seen after co-treatment with IGF-II and insulin. There were significantly more PCNA-positive follicles in IGF-I cultures than in control cultures. These results suggest that insulin, IGF-I and IGF-II may act as survival factors for early stage human follicles. IGFs may also be involved in activation of the mitotic cell cycle of granulosa cells.     

瘦素、纤连蛋白对早孕绒毛滋养细胞分泌基质金属蛋白酶的影响

目的探讨瘦素、纤连蛋白对早孕绒毛滋养细胞分泌基质金属蛋白酶的影响及其作用。方法从孕6-8周的人工流产胚胎绒毛中分离、纯化出细胞滋养细胞(CTB)体外培养,在transwell上室CTB培养液中分别加入不同浓度lep-tin,在transwell下室中加入50μg/mlFN,采用明胶酶谱分析法检测培养液中CTB分泌的MMP-2、MMP-9值并进行比较。结果FN组、协同组分泌的MMP-2,9显著高于对照组(P0.05),协同组又高于单加leptin或FN组(P0.05)。结论leptin、FN均具有促进CTB分泌MMPs的作用,且leptin与FN协同作用,更加强了CTB对MMPs的分泌,从而参予调控CTB的浸润能力。     

体外受精-胚胎移植中卵巢低反应80例分析

目的探讨体外受精-胚胎移植（IVF—ET）中卵巢低反应的预测及结局。方法回顾性分析2005—2007年在本生殖中心首次接受IVF—ET治疗的不孕患者,以促性腺激素（Gn）刺激后两侧卵巢发育卵泡≤3个,或经阴道超声引导取卵,获卵数≤4个为卵巢低反应判断标准,符合卵巢低反应标准的80例为低反应组,取同期获卵5—20个的80个IVF—ET治疗周期为对照组。分析两组平均年龄,年龄≥35岁、双侧卵巢窦卵泡数≤5个、基础促卵泡素（bFSH）≥8IU／L、基础雌二醇（bE2）≥80μg／L患者的比例,周期取消率,平均Gn用量及临床妊娠率。结果低反应组平均年龄,年龄≥35岁、bFSH≥8IU／L患者的比例均明显高于对照组（P〈0．05）;低反应双侧卵巢窦卵泡数≤5个及周期取消率明显高于对照组,相差非常显著（P〈0．01）;两组bE：≥80μg/L患者的百分比及平均Gn用量无显著性差异（P〉0．05）;低反应组临床妊娠率（21．25％）明显低于对照组（41．03％）,差异有统计学意义（P〈0．05）。结论患者年龄≥35岁、bFSH≥8 IU／L及窦卵泡≤5个均可作为预测卵巢低反应的指标。