五子衍宗丸中五味子醇甲的含量测定

目的建立五子衍宗丸中五味子醇甲含量的测定方法。方法采用高效液相色谱法,色谱柱为钻石ODS柱(200mm×4.6mm,5μm),流动相为甲醇-水(64∶36),流速为1.0mL/min,检测波长为250nm,柱温为室温。结果五味子醇甲进样量线性范围是0.1076～1.614μg,r=0.9999,方法平均加样回收率为98.03%,RSD=1.04%(n=9)。结论该方法可用于测定五子衍宗丸中五味子醇甲的含量。     

HPLC法测定肝复康丸中五味子醇甲的含量

目的 建立肝复康丸中五味子醇甲的含量测定方法.方法 采用高效液相色谱法,色谱柱VP-ODS柱(150 mm×4.μm);流动相:乙腈-甲醇-水(15:15:10),检测波长250 nm,流速1.0ral·min-1,柱温室温.结果 回归方程Y=1.002 1.77×104x(r=0.9999,n=5),五味子醇甲在0.08～1.20μg范围内线性良好,加样回收率99.67%,RSD=1.24%.结论 本法灵敏度高,操作简便,重复性好,准确可靠,可作为肝复康丸中五味子醇甲的含量测定方法.     

高效液相色谱法测定复方活脑舒胶囊中五味子醇甲含量

目的:建立复方活脑舒胶囊中五味子醇甲含量的测定方法.方法:选用色谱柱KromasiLC18柱(250 mm×4.6 mm,5μm),以乙腈0.5%磷酸溶液(45:55)为流动相,流速为0.6 mL/min,检测波长为250 nm,柱温30℃.结果:五味子醇甲进样量在0.082～0.41μg范围内与峰面积线性关系良好(r=1.000),平均回收率为100.71%,RSD为1.48%.结论:方法简便、准确,重现性好,可作为该制剂的含量测定方法.     

护肝颗粒中五味子醇甲含量测定方法研究

目的:制定护肝颗粒中五味子醇甲含量测定方法。方法:采用HPLC法,色谱柱为C18,流动相为甲醇-水(63:37);检测波长250nm。结果:五味子醇甲在0.02052μg~0.513μg范围内,进样量与峰面积线性关系良好(r=1.0000)。加样回收率为98.7%(RSD为0.6%)。结论:此方法准确可靠、重复性好,可用于护肝颗粒中五味子醇甲的含量测定。     

高效液相色谱法测定屏风生脉胶囊中五味子醇甲的含量

目的:建立屏风生脉胶囊中五味子醇甲的含量测定方法.方法:采用高效液相色谱法.色谱柱为Inertsil ODS-3(4.6 mm×150 mm,5μm),流动相为乙腈-水(45:55),流速为1.0 mL·min-1,检测波长为250 nm,柱温为25℃.结果:五味子醇甲进样量在0.1～1.1μg内呈良好的线形关系(r=0.9996),平均回收率为95.59%,RSD为1.21%.结论:本方法简便,准确,专属性强,重复性好,可用于屏风生脉胶囊中五味子醇甲含量的测定.     

高效液相色谱法测定五子衍宗胶囊中五味子醇甲的含量

目的建立五子衍宗胶囊中五味子醇甲的高效液相色谱含量测定方法.方法采用Kromasil C18(150 mm×4.6 mm,5μm)色谱柱;流动相为甲醇-水(5842);流速为1.0 mL·min-1,柱温25℃,检测波长250 nm.结果五味子醇甲在27.79～46.32 mg·L-1范围内,呈良好的线性关系,回归方程为Y=16 627X-1 474.8,r=1.0000,平均回收率为99.21%,RSD为0.57%.结论本方法灵敏度高,准确,重复性好,可作为五子衍宗胶囊中五味子醇甲含量测定方法.     

RP-HPLC测定保精颗粒中五味子醇甲的含量

目的 建立保精颗粒中五味子醇甲的含量测定方法.方法 采用高效液相色谱法,Kromasil C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-水(45∶55),流速1.0 mL· min-1,检测波长250 nm.结果 方法学考察结果表明,五味子醇甲平均加样回收率为100.7％(RSD=1.3％,n=6).结论 该方法专属性强、重复性好,可作为保精颗粒的质量控制方法.     

HPLC法测定复方五味子补肾颗粒中的五味子醇甲的含量

目的建立复方五味子补肾颗粒中五味子醇甲的含量测定方法。方法采用高效液相色谱法测定复方五味子补肾颗粒中五味子醇甲的含量。Welch Materials C18色谱柱(4.6 mm×250 mm,5μm);甲醇-水(70∶30)为流动相;流速:1.0 ml.min-1;柱温25℃。结果在高效液相色谱法图中,五味子醇甲与其它组份分离良好,线性范围为0.024 4～0.244 g.L-1,其回归方程为:Y=39 648.426X+51.18,r=0.999 9(n=6),线性良好为0.024 4～0.244 g.L-1,平均加样回收率为99.46%(RSD=0.54%)。结论该方法可以很好的用于控制复方五味子补肾颗粒的质量。     

五香血藤中五味子醇甲与五味子甲素含量测定

目的建立同时测定五香血藤中五味子醇甲与五味子甲素含量的高效液相色谱法。方法采用反相高效液相色谱法,C18色谱柱(4.6 mm×250 mm,5μm),乙腈(A)-水(B)为流动相,梯度洗脱,流速1 mL.min-1,五味子醇甲检测波长216 nm,五味子甲素检测波长209 nm。结果五味子醇甲在0.019 6～0.215 6μg之间线性关系良好,回收率99.11%,RSD=2.02%(n=9);五味子甲素在0.018 4～0.147 2μg之间线性关系良好,回收率102.35%,RSD=1.73%(n=9)。结论该方法简便、准确,可用于五香血藤中五味子醇甲与五味子甲素的含量测定。     

HPLC法测定八味肾气丸中五味子醇甲含量的研究

目的建立八味肾气丸中五味子醇甲含量测定的高效液相色谱法。方法采用C18柱(KR100-5C18,4,6mm×250mm,5μm),甲醇-水(65∶35)为流动相;流动速为1.0mL.min-1;检测波长为250nm。结果五味子醇甲在0.105~1.05μg范围内,线性关系良好(r=0.9999),方法平均回收率为97.82%,RSD=1.13%(n=6)。结论方法简便,结果准确,可用于八味肾气丸中五味子醇甲的定量。     

Efficacy of gut lavage,hemodialysis, and hemoperfusion in the therapy of paraquat or diquat intoxication

Clinical and in vitro investigations were carried out to test the efficacy of gut lavage, hemodialysis, and hemoperfusion in the treatment of poisoning with paraquat or diquat. In a patient suffering from diquat intoxication 130 times more diquat was removed by gut lavage 30 h after ingestion than was removed by complete aspiration of the gastric contents.Determination of in vitro clearances for paraquat and diquat by hemodialysis showed that, at serum concentrations of 1–2 ppm, such as are frequently encountered in poisoning in man, toxicologically relevant quantities of herbicide cannot be removed from the body. At a concentration of 20 ppm, on the other hand, hemodialysis proved to be effective, the clearance being 70 ml/min at a blood flow rate of 100 ml/min. The efficacy of hemoperfusion with coated activated charcoal was on the whole better. Especially at concentrations around 1–2 ppm, the clearance values for hemoperfusion were some 5–7 times higher than those for hemodialysis.In a patient suffering from paraquat poisoning, both hemodialysis as well as hemoperfusion were carried out. The in vitro results could be confirmed: At serum concentrations of paraquat less than 1 ppm no clearance could be obtained by hemodialysis while by hemoperfusion with activated charcoal quite high clearance values were measured and the serum level dropped down to zero.

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Zusammenfassung Klinische Untersuchungen und Laboratoriumsversuche wurden durchgeführt, um die Wirksamkeit von Darmspülung, Hämodialyse und Hämoperfusion bei Paraquat- und Deiquat-Vergiftungen zu prüfen.Bei einem Patienten wurde 30 Std nach Deiquat-Aufnahme durch Darmspülung 130mal mehr Deiquat entfernt als durch vollständige Aspiration des Mageninhaltes. In vitro-Versuche ergaben, daß bei Blutserumkonzentrationen von 1–2 ppm, die bei Vergiftungen oft gemessen werden, durch Hämodialyse keine toxikologisch relevanten Paraquat- oder Deiquat-Mengen entfernt werden können. Dagegen erwies sich die Hämodialyse bei 20 ppm und einer Blutumlaufgeschwindigkeit von 100 ml/min mit einer Clearance von 70 ml/min als wirksam. Die Hämoperfusion mit beschicheter Aktivkohle war in diesen Versuchen aber eindeutig überlegen, denn insbesondere bei Konzentrationen um 1–2 ppm waren die Clearance-Werte 5–7mal höher als bei der Hämodialyse.Die in vitro-Ergebnisse wurden bei einem Patienten mit einer Paraquat-Vergiftung bestätigt: Bei Konzentrationen unter 1 ppm war die Hämodialyse wirkungslos, während durch Hämoperfusion relativ hohe Clearance-Werte erreicht wurden, so daß der Serumspiegel rasch unter die Nachweisgrenze abfiel.

     

In vitro studies on sterically stabilized liposomes (SL) as enzyme carriers in organophosphorus (OP) antagonism

This study describes a new approach for organophosphorous (OP) antidotal treatment by encapsulating an OP hydrolyzing enzyme, OPA anhydrolase (OPAA), within sterically stabilized liposomes. The recombinant OPAA enzyme was derived from Alteromonas strain JD6. It has broad substrate specificity to a wide range of OP compounds: DFP and the nerve agents, soman and sarin. Liposomes encapsulating OPAA (SL)* were made by mechanical dispersion method. Hydrolysis of DFP by (SL)* was measured by following an increase of fluoride ion concentration using a fluoride ion selective electrode. OPAA entrapped in the carrier liposomes rapidly hydrolyze DFP, with the rate of DFP hydrolysis directly proportional to the amount of (SL)* added to the solution. Liposomal carriers containing no enzyme did not hydrolyze DFP. The reaction was linear and the rate of hydrolysis was first order in the substrate. This enzyme carrier system serves as a biodegradable protective environment for the recombinant OP-metabolizing enzyme, OPAA, resulting in prolongation of enzymatic concentration in the body. These studies suggest that the protection of OP intoxication can be strikingly enhanced by adding OPAA encapsulated within (SL)* to pralidoxime and atropine.     

Aquatic Insects and Trace Metals: Bioavailability,Bioaccumulation, and Toxicity

Abstract

The uptake of metals from food and water sources by insects is thought to be additive. For a given metal, the proportions taken up from water and food will depend both on the bioavailable concentration of the metal associated with each source and the mechanism and rate by which the metal enters the insect. Attempts to correlate insect trace metal concentrations with the trophic level of insects should be made with a knowledge of the feeding relationships of the individual taxa concerned. Pathways for the uptake of essential metals, such as copper and zinc, exist at the cellular level, and other nonessential metals, such as cadmium, also appear to enter via these routes. Within cells, trace metals can be bound to proteins or stored in granules. The internal distribution of metals among body tissues is very heterogeneous, and distribution patterns tend to be both metal and taxon specific. Trace metals associated with insects can be both bound on the surface of their chitinous exoskeleton and incorporated into body tissues. The quantities of trace meals accumulated by an individual reflect the net balance between the rate of metal influx from both dissolved and particulate sources and the rate of metal efflux from the organism. The toxicity of metals has been demonstrated at all levels of biological organization: cell, tissue, individual, population, and community. Much of the literature pertaining to the toxic effects of metals on aquatic insects is based on laboratory observations and, as such, it is difficult to extrapolate the data to insects in nature. The few experimental studies in nature suggest that trace metal contaminants can affect both the distribution and the abundance of aquatic insects. Insects have a largely unexploited potential as biomonitors of metal contamination in nature. A better understanding of the physico-chemical and biological mechanisms mediating trace metal bioavailability and exchange will facilitate the development of general predictive models relating trace metal concentrations in insects to those in their environment. Such models will facilitate the use of insects as contaminant biomonitors.     

Steroidal sapogenin contents in some domestic plants

In order to find out the values of the steroid resources for the future use. the compositions and contents of steroidal sapogenins from 13 domestic plants have been investigated. As a result,Dioscorea nipponica, D. quinqueloba andSmilax china were found to have large amount of diosgenin. And pennogenin inTrillium kamtschaticum andParis verticillata, yuccagenin inAllium fistulosum, hecogenin inAgave americana and neochlorogenin inSolanum nigum were appeared to be major steroidal sapogenins.     

Alzheimer’s disease – current trends in basic and clinical research. Highlights from the 16th ECNP

Advances in the molecular biological knowledge of neuronal nicotinic acetylcholine receptors (nAChRs) have led to a growing interest by the pharmaceutical industry in the development of novel compounds that selectively modulate nAChR function. The ability of (-)-nicotine, an activator of nAChRs, to enhance attentional aspects of cognition in animals and humans, to exert neuroprotective and anxiolytic-like effects, and presumably to mediate the negative correlation between smoking and Alzheimer's (and Parkinson's) Disease, has focused interest on the potential therapeutic utility of modulators of nAChR function for treatment of some of the deficits associated with these progressive, neurodegenerative conditions. Numerous compounds are known which activate nAChRs and which might serve as lead compounds toward the development of such agents. The pharmacologic diversity of neuronal nAChR subtypes suggests the possibility of developing selective compounds which would have more favourable side-effect profiles than existing agents. This broader class of agents, collectively called cholinergic channel modulators (ChCMs), is anticipated to encompass compounds which would have more favourable side-effect profiles than existing agents, which generally exhibit low selectivity. This selectivity may be achieved by preferentially activating some subtypes of nAChRs (i.e., Cholinergic Channel Activators, ChCAs) or inhibiting the function of other subtypes (Cholinergic Channel Inhibitors, ChCIs). An overview of the biology of nAChRs and the rationale for the use of ChCMs for the treatment of dementia related to neurodegenerative diseases are presented, followed by a discussion of lead compounds and compounds under consideration for clinical evaluation.