林荫千里光的化学成分

为了研究林荫千里光Senecio nemorensis.的化学成分,从林荫千里光中分离鉴定了12个已知化合物,分别为烟酰胺(Ⅰ),香草醛(Ⅱ),丁香酸(Ⅲ),丁香醛(Ⅳ),3-乙酰基-4-羟基苯甲酸(Ⅴ),4,4-二甲基-1,7-庚二酸(Ⅵ)3-醛基吲哚(Ⅶ),咖啡酸乙酯(Ⅷ),对-甲氧基桂皮酸葡萄糖酯(Ⅸ),(6S,7E)-6-hydroxy-4,7-megastigmadien-3,9-dione(Ⅹ),Annuion-one D(Ⅺ)和(1′S,6′R)-abscisic acid(Ⅻ)。     

匍枝千里光的生物碱研究

目的对千里光属中匍枝千里光的生物碱进行研究。方法使用常压硅胶柱层析法对匍枝千里光乙醇提取物的二氯甲烷萃取部分的化学成分进行分离和纯化,通过理化方法和核磁共振(NMR)对分离得到的化合物进行结构鉴定。结果从匍枝千里光中分离并鉴定了3个新的生物碱(人工产物)N-氯甲基-9-当归酰矛蟹甲草裂碱氯化物(P-1)、N-氯甲基瓶草千里光碱氯化物(P-2)、N-氯甲基瓶草千里光新碱氯化物(P-3)和1个已知的吡咯里西啶生物碱9-当归酰阔叶千里光裂碱(P-4)。结论 4个吡咯里西啶生物碱化合物均为首次从匍枝千里光中经二氯甲烷处理后分离得到。     

九节菖蒲的化学成分研究

目的:研究九节菖蒲的化学成分.方法:利用Diaion HP-20,Toyopearl HW-40,Sephadex LH-20,硅胶等柱色谱技术对九节菖蒲的化学成分进行分离纯化,根据化合物的光谱数据和理化性质鉴定其结构.结果:分离并鉴定了9个化合物的结构,分别为:七叶内酯(Ⅰ),氢化咖啡酸(Ⅱ),阿魏酸(Ⅲ),氢化阿魏酸(Ⅳ),5-羟甲基糠醛(V),3,4-二羟基苄醇(Ⅵ),9,12,13-三羟基-10,15-十八碳二烯酸(Ⅶ),5-羟基-4氧代戊酸(Ⅷ),β-·谷甾醇(Ⅸ).结论:化合物(Ⅰ)-(Ⅶ)均为首次从该植物中分得.     

披针新月蕨细胞毒活性成分研究

目的 研究民族药披针新月蕨Abacopter is penangiana化学成分并评价其细胞毒活性。方法 以硅胶柱、凝胶柱色谱分离,制备HPLC纯化,采用MS,1、2 D-NMR等波谱方法进行结构鉴定,MTT法测试各化合物的细胞毒活性。结果 从披针新月蕨中分离鉴定了6个化合物,分别为4(S),5,7-三羟基-4′-甲氧基-6,8-二甲基-2(R)-黄烷-5,7-O-β-D-二葡萄糖苷［4(S),5,7-trihydroxy-4′-methoxy-6,8-dimethyl-2(R)-flavan-5,7-O-β-D-diglucopyranoside, eruberin B, 1］; 5,7-二羟基-4(S),4′-二甲氧基-6,8-二甲基-2(R)-黄烷-5,7-O-β-D-二葡萄糖苷［5,7-dihydroxy-4(S),4′-dimethoxy-6,8-dimethyl-2 (R)-flavan-5,7-O-β-D-diglucopyranoside,eruberin C, 2］;5,7-二羟基-4′-甲氧基-6-羟甲基-8-甲基-2″,4(S)-氧-2(R)-黄烷-5-O-β-D-葡萄糖苷［5,7-dihydroxy-4′-methoxy-6-hydroxymethyl-8-methyl-2″,4(S)-oxido-2(R)-flavan-5-O-β-D-glucopyranoside,3］;5,7-二羟基-4′-甲氧基-6,8-二甲基-2″,4(S)-氧-2(R)-黄烷-5-β-D-葡萄糖苷［5,7-dihydroxy-4′-methoxy-6,8-dimethyl-2″,4(S)-oxido-2(R)-flavan-5-O-β-D-glucopy ranoside,eruberin A,4］;高圣草素［5,7,4′-trihydroxy-3′-methoxyflavanone, homoeriodictyol,5］;对-甲氧基桂皮醛(p-methoxycinnamaldehyde,6)。化合物3、4对L929及HeLa细胞的IC50值分别约为13.05、24.75 μg/mL,25.11、18.32 μg/mL。结论 化合物3为新的天然产物,化合物2、5、6首次从新月蕨属植物中分得,化合物3、4对L929和HeLa具有显著抑制活性。     

野花椒中一个新的单萜苷

目的对野花椒Zanthoxylum simulans根的化学成分进行研究。方法用柱色谱技术对化合物进行分离纯化,并用光谱技术和理化性质鉴定化合物的结构。结果从野花椒根乙醇提取物分离得到1个新的单萜苷和4个已知化合物,分别鉴定为(1R,2R,4S)-2-羟基-1,8-桉树脑-2-O-α-D-芹菜糖基(1→6)-β-D-葡萄糖苷[(1R,2R,4S)-2-hydroxy-1,8-cineole-2-O-α-D-apiofuranosyl-(1→6)-β-D-glucopyranoside,Ⅰ]、淫羊藿苷E5(icarisideE5,Ⅱ)、巨盘木碱(flindersine,Ⅲ)、N-甲基-巨盘木碱(N-methylflindersine,Ⅳ)、胡萝卜苷(daucosterol,Ⅴ)。结论化合物Ⅰ为新化合物,命名为野花椒苷A(zansiumloside A)。化合物为首次从该属植物中分离得到。     

国产贯叶连翘化学成分的研究

目的：对我国贵州产贯叶连翘的化学成分进行了较系统的化学研究。方法：利用各种色谱技术进行分离纯化，经理化常数和波谱分析等进行结构鉴定。结果：分离得到8个化合物，这些化合物分别鉴定为：槲皮素（quercetin,I)，槲皮苷（quercitrin,Ⅱ），山柰酚（kaempferol,Ⅲ),1,7-二羟基山酮（1，7－dihydroxyxanthone,Ⅳ),kielcorin(5-hydroxymethyl-6-guaiacyl-2,3:3‘,2‘,4‘-methoxy-xanthone-1,4-dioxane,Ⅴ),大黄素（emodin,Ⅵ),2,5-二甲基－7－羟基色原酮（2，5－dimethyl-7-hydroxychromone,Ⅶ)和香草酸（vanillic acid,Ⅶ)。结论：化合物Ⅳ，Ⅶ和Ⅷ为首次从贯叶连翘中分离得到。     

麻叶千里光抗菌化学成分的研究(Ⅱ)

目的研究麻叶千里光S enecio cannabif olius抗菌活性成分。方法以革兰氏阳性菌金黄色葡萄球菌S taphy lococcus aureus、枯草芽孢杆菌B acillus subtilis和阴性菌大肠杆菌E scherich ia coli为受试菌,采用体外抑菌圈法追踪抗菌活性部分,利用各种色谱技术进行分离纯化,根据理化性质和谱学数据进行结构鉴定。结果从麻叶千里光中分离得到5个化合物,分别鉴定为5-羟基吡啶-2-甲酸甲酯(E-13)、6-羟基-7,7a-二氢-2-(6H)-苯骈呋喃酮(E-14)、2-(1,4-二羟基环己烷基)-乙酸(E-16)、3-羟基环己酸(E-17)、4-羟基苯甲醛(B-14)。结论它们均为从千里光属植物中首次分到。     

石蝉草化学成分的研究

目的研究草胡椒属植物石蝉草Peperomiadindygulensis的化学成分。方法用色谱法分离化合物,根据理化性质和光谱数据鉴定结构。结果从石蝉草的氯仿部位分离得到了8个化合物,分别鉴定为:二(2-甲氧基-4,5-亚甲二氧基)-苯基酮(Ⅰ)、草胡椒素B(peperomin B,Ⅱ)、草胡椒素C(peperomin C,Ⅲ)、5-羟基-4′,7,8-三甲氧基黄酮(Ⅳ)、5-羟基-3′,4′,7,8-四甲氧基黄酮(Ⅴ)、5,3′-二羟基-4′,7,8-三甲氧基黄酮(Ⅵ)、β-谷甾醇(Ⅶ)、正十六酸(Ⅷ)。结论化合物Ⅰ为新化合物,命名为石蝉草素(dindygulensin)。除化合物Ⅴ外,其余成分均为首次从该种植物中分离得到。     

皱皮木瓜的化学成分研究Ⅱ

目的:研究皱皮木瓜的化学成分.方法:利用多种色谱法对皱皮木瓜的化学成分进行系统研究,并根据理化性质和波谱数据进行结构鉴定.结果:从皱皮木瓜的乙酸乙酯萃取部位分离鉴定了9种化合物,分别为绿原酸甲酯(Ⅰ),5-O-咖啡酰基-奎宁酸丁酯(Ⅱ),奎宁酸丁酯(Ⅲ),5-羟甲基-2-糠醛(Ⅳ),三十烷酸(Ⅴ),(-)-表儿茶素(Ⅵ),7,8-二羟基香豆素(Ⅶ),3,4-二羟基苯甲酸乙酯(Ⅷ),原儿茶酸(Ⅸ).结论:化合物Ⅲ～Ⅷ均为从本属植物中首次分离得到.     

青梅茎的化学成分

从青梅(Vatica mangachpoi Blanco)的茎中分离鉴定了10种成分:豆甾-4-烯-3-酮(Ⅰ),3-羟基-4-甲氧基苯甲酸(Ⅱ),丁香醛(Ⅲ),东莨菪内酯(Ⅳ),对羟基苯甲醛(Ⅴ),2,6-二甲氧基苯醌(Ⅵ),4-羟基-3,5-二甲氧基苯丙烯醛(Ⅶ),积雪草酸(Ⅷ),3-(1-C-β-葡萄糖)-2,6-二羟基-5-甲氧基苯甲酸(Ⅸ),1,3,3a,4,9,9a-hexahydro-4-(4-hydroxy-3,5-dimethoxy-phenyl)-5,7-dimethoxynaphtho[2,3-c]furan-6-ol(Ⅹ)。其中化合物Ⅰ、Ⅱ、Ⅳ、Ⅵ、Ⅶ、Ⅷ、Ⅸ、Ⅹ为首次从该属植物中分离得到。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.