复方脑栓1号治疗脑血栓对甲襞微循环及血液流变性的影响

通过对１００例脑血栓患者随机分组对比治疗,观察组５０例采用复方 脑栓１号治疗２个疗程,对照组５０例采用维脑路通治疗２个疗程,观察治疗前后 甲襞微循环和血液流变性的变化及疗效。结果表明：复方脑栓１号,有显著改善甲 襞微循环和血液流变性的作用,治疗总有效率９６％,治愈率５２％,均优于维脑路通 （Ｐ＜０．０５）。     

红细胞聚集对血细胞分析仪检测结果的影响及处理

目的探讨RBC发生聚集对RBC相关参数的影响,并提出解决方法。方法将疑为RBC聚集的标本离心后,用等量生理盐水置换标本血浆后再次上机检测。结果 RBC聚集导致RBC计数明显降低,红细胞压积(HCT)、平均红细胞容积(MCV)、平均血红蛋白分量(MCH)、平均红细胞血红蛋白浓度(MCHC)参数均不可靠。其中MCHC明显增高为典型表现。经处理后,结果恢复正常。结论 RBC聚集严重影响RBC相关参数的检测,本方法可以消除因血浆因素对RBC相关参数的影响。     

对血细胞分析仪提示血小板聚集标本的临床意义探讨

近年来,血栓性疾病的研究日益引起国内外医学界的重视。本文对1999年4月-2000年3月本院的562例住院患者静脉血,引起血细胞分析仪WBC、PLT直方图及参数异常,并提示血小板聚集的标本,在排除有核红细胞、冷凝集素、红细胞碎片、大血小板等因素的影响后,进行疾病分析,并作了手工法对比,现报道如下。     

EDTA-K2诱导的血小板聚集对血细胞计数的影响

目的 研究EDTA-K2诱导血小板聚集所引起的血液分析仪计数RBC、WBC及PLT的数值变化.方法 采用血液分析仪检测EDTA-K2抗凝血,同时手工计数标本和涂片镜检,并作相应比较得出结论.结果 血液分析仪检测的部分EDTA-K2抗凝血血小板计教低于手工法计数的结果,而白细胞计数结果却高于后者,二者差别均有临床意义.结论 时于EDTA-K2抗凝的静脉血个别患者可能会发生血小板聚集,从而引起血小板假性减少PTCP(pseudothrombocytopenia),WBC计数假性增高,所以应该引起重视,及时纠正,避免一些不必要的检查和治疗.     

前列腺E1对冠心病患者微循环和血细胞流变学障碍的治疗作用

目的研究前列腺素E1对30例冠心病患者微循环和血细胞流变学障碍的治疗作用。方法观察前列腺素E1治疗前后冠心病患者甲襞微循环改变的同时采指端血,制成活血片,用高放大多功能显微生物诊断仪测定活血细胞流变特性的变化。结果治疗前,冠心病患者甲襞微血管数目5.87±0.92条/mm;输入枝口径5.75±0.63um;红细胞聚集率90.65±5.35、血小板聚集率86.58±9.64、活化率60.55±10.35;白细胞聚集率35.45±5.25、活化率86.35±12.46。治疗后,甲襞微血管数目6.92±0.51条/mm;输入枝口径7.53±0.34um;红细胞聚集率12.68±4.38、活化率34.78±7.54;白细胞聚集率12.68±4.38、活化率30.69±6.54。经比较,前列腺素E1治疗后冠心病患者甲襞微血管数目明显增多;输入枝口径扩张(P<0.05～0.01);红细胞聚集率;血小板聚集率、活化率;白细胞聚集率、活化率均明显降低(P<0.05～0.01)。结论前列腺素E1性能稳定、无血管刺激副作用;具有扩张微血管、增加微血管密度;抗血小板聚集活化、改善血细胞流变学特性;疗效显著。     

前列腺素E1对冠心病患者微循环和血细胞流变学障碍的治疗作用

目的研究前列腺素E1对30例冠心病患者微循环和血细胞流变学障碍的治疗作用.方法观察前列腺素E1治疗前后冠心病患者甲襞微循环改变的同时采指端血,制成活血片,用高放大多功能显微生物诊断仪测定活血细胞流变特性的变化.结果冠心病患者治疗前后,甲襞微血管数目5.87±0.92对6.92±0.51条/mm;输入枝口径5.75±0.63对7.53±0.34um;红细胞聚集率90.65±35.35对52.55±4.36、血小板聚集率86.58±9.64对47.59±5.32、活化率60.55±10.35对34.78±7.54;白细胞聚集率35.45±5.25对12.68±4.38、活化率循环内斑块869.28±102.51对125.46±53.57um2(P均〈0.05-0.01).结论前列腺素E1具有扩张微血管、保护血管内皮细胞;抗血小板、白细胞聚集、活化,改善红细胞聚集及变形能力;增加微血管密度,改善组织血供.     

1例胞二磷胆碱与维脑路通联合用药致过敏性休克的护理

胞二磷胆碱为核苷类药物,通过增加脑组织血流而促进大脑物质代谢,对促进大脑功能恢复和促进苏醒等具有一定作用。维脑路通（曲克芦丁）注射液能抑制血小板聚集,有防止血栓形成、对抗5-羟色胺、增加毛细血管抵抗力、降低毛细血管通透性、改善微循环等功效。     

1例胞二磷胆碱与维脑路通联合用药致过敏性休克的护理

胞二磷胆碱为核苷类药物,通过增加脑组织血流而促进大脑物质代谢,对促进大脑功能恢复和促进苏醒等具有一定作用[1].维脑路通(曲克芦丁)注射液能抑制血小板聚集,有防止血栓形成、对抗5-羟色胺、增加毛细血管抵抗力、降低毛细血管通透性、改善微循环等功效[2].临床上常将这2种药物联合用于治疗脑卒中、脑血栓形成及其他脑病,偶有过敏反应,少见严重不良反应.     

噬血细胞综合征继发凝血功能障碍的认识与研究

噬血细胞综合征(HPS)是淋巴细胞和组织细胞非恶性增生,产生细胞因子风暴所导致的一种临床综合征.临床表现多样化,其中凝血功能障碍与HPS患者高病死率密切相关.作者对噬血细胞综合征患者发生凝血功能障碍的病理生理机制、实验室表现、临床表现及治疗加以综述,以期提高对噬血细胞综合征并发凝血功能障碍的认识,从而及时发现并治疗凝血功能障碍,降低早期病死率,改善预后.     

脑瘫1号治疗福利院脑瘫儿童临床疗效观察

目的探讨中药脑瘫1号治疗福利院儿童脑瘫临床疗效。方法福利院脑瘫儿童120例分成实验组和对照组,实验组口服"脑瘫1号",对照组服用脑复康。结果实验组和对照组总有效率分别为93.33%、78.67%(P<0.05)。结论中药脑瘫1号对儿童脑瘫有效。     

血液保存时间对库存血流变特性的影响

目的：观察血液保存时间对血液流变特性的影响。方法：选择５６例 健康献血员。常规采血及保存,取新鲜血（采集后１小时）、１周上周及３周４个时相 点。检测全血粘度（高切、中切、低切）、血浆粘度、血球压积及ＲＢＣ聚集指数（ＡＩ） 和刚性指数（ＩＲ）。结果：红细胞压积、中切粘度在保存３周内无显著变化,高切粘度 在第三周显著升高,而低切粘度下降。血浆粘度随保存时间延长进行性下降。ＡＩ在 第３周显著降低而ＩＲ在第２周时则升高,第３周更为明显。结论：结果提示库存血 保存时间的延长对全血粘度和 ＲＢＣ的变形性及聚集性产生显著影响,特别在第 ３ 周出现非常显著变化,ＲＢＣ变形性改变影响ＲＢＣ的运氧功能,降低了输血效果。     

肾上腺素能受体在脾脏红细胞储存与释放的动力机制中的作用

通过对狗的脾脏进行α受体阻断情况下的低浓度肾上腺素溶液在体灌注实验，探讨了脾脏储存与释放血细胞的微循环动力机制。实验结果支持Ｇｒｏｏｍ提出的脾索内肌质纤维友交感神经控制下和血液调节机制，并认为脾索及静脉窦壁的过滤作用是调节脾内红细胞浓度的主要因素，但并非如Ｍａｃｋｅｎｚｉｅ和ＪΦｒｇｅｎ等人认为的红细胞被动地滞留于脾索中，而是存在交感神经及血液中儿荷胺调控下的主动机制。     

人脐血血清对人骨髓基质细胞增殖的实验研究

目的　探究人脐血血清对人骨髓基质细胞增殖的作用。方法　无菌条件下抽取新鲜脐血 ,经肝素抗凝、离心分离血清、灭活补体、微孔滤膜过滤制备人脐血血清 ,细胞按照一定浓度接种于DMEM / 2 0 %人脐血血清培养基 ,定期观察细胞生长并更换培养基。结果　细胞接种 2 4h后有少量梭形细胞贴壁 ,2周时细胞基本长满瓶底 ,细胞呈漩涡状排列。结论　在体外人骨髓基质细胞可在含人脐血血清的DMEM中增殖     

传统活血化瘀药对红细胞流变学特性影响的比较研究

文中试图通过３４种活血化瘀药对红细胞流变学特性影响的比较研究，专门探索３类作用强度不同的活血化瘀药对红细胞聚集性、变形性的作用，为临床应用活血化瘀药提供实验依据，亦为血液流变性研究增添新的内容．     

THE EFFECT OF TESTICLE EXTRACT ON RED BLOOD CELLS IN VITRO

Testicle extract possesses a notable power to increase red cell fragility. Testicle extracts of the rat, rabbit and guinea pig all exhibit this property, which is most pronounced with the extract from the rat and least so with that from the guinea pig. Splenic extract does not possess the property, or possesses it only to a very slight degree. These results support the hypothesis that the factor in testicle extract which enhances infections (the Reynals factor) does so by altering the permeability of the host tissue.     

自体LAK细胞对白血病细胞清除作用的实验研究

采用￣（５１）Ｃｒ４小时释放试验，细胞培养及组化分析技术，研究了２６例初诊急性髓细胞白血病患者（ＡＰｓ）和其中的１２例缓解期患者（ＲＰｓ）的ＬＡＫ细胞对自体白血病细胞（Ａｕｔｏ－ＬＢ）的清除作用。ＡＰｓ的外周血或骨髓通过双层Ｆｉｃｏｌｌ－Ｈｙｐａｑｕｅ分离所得富含淋巴细胞层中含２０％～５０％的Ａｕｔｏ－ＬＢ，在ｒＩＬ－２１０００Ｕ／ｍｌ的培养基中培养１～２周Ａｕｔｏ－ＬＢ消失。而￣（５１）Ｃｒ４小时释放试验的结果是ＡＰｓ－ＬＡＫ和ＲＰｓ－ＬＡＫ细胞胞对Ａｕｔｏ－ＬＢ的杀伤活性分别为１２．９％±１２．３％和２４．１％±１２．０％，均较其对Ｒａｊｉ细胞的杀伤活性（３６．０％±３２．０％和６６．２％±１８．１％）显著减低（Ｐ＜０．０１），提示：Ａｕｔｏ－ＬＢ细胞表面能被ＬＡＫ细胞识别的决定簇较Ｒａｊｉ细胞系低。但体外培养过程中Ａｕｔｏ－ＬＢ能被清除。提示：清除机制除了有ＬＡＫ细胞的直接杀伤外，尚有其它机制参与，体外培养过程中Ａｕｔｏ－ＬＢ表面决定簇表达增加。     

交替半身照射清除大鼠体内残留白血病细胞作用的研究

为了进一步提高交替半身照射（ＨＢＩ）清除体内残留白血病细胞的作用，本研究用大鼠ＢＮＭＬ白血病的亲代细胞进行了实验，进一步证实环磷酰胺（ＣＴＸ）加交替ＨＢＩ治疗，不仅可不用骨髓移植即能重建造血而且能延长生存时间，部分大鼠获得治愈。同时，还探讨了不同的ＣＴＸ＋交替ＨＢＩ治疗方案对疗效的影响，证明交替ＨＢＩ的疗效与化疗预处理的效果、开始照射的时间以及两次照射间隔时间有密切的关系，提出了最佳治疗方案，为该治疗方案用于临床提供了实验依据。     

STUDIES UPON THE EFFECT OF LIGHT ON BLOOD AND TISSUE CELLS : I. THE ACTION OF LIGHT ON WHITE BLOOD CELLS IN VITRO.

1. An extreme and rapid degeneration which occurred in tissue cultures of leucocytes from the blood of cats, guinea pigs, and rabbits, is described in detail. 2. This degeneration was found to appear in the culture when the cells were planted in any of the culture media tried, some of which were autogenous heparin plasma, autogenous plasma, autogenous serum, Tyrode solution, and mixtures of these with embryo juice. 3. The specific cellular changes which occurred are described for the different leucocytes. In general, there was first a latent period during which no change could be observed in the cell. Following this there was a period of stimulation during which the motion of the cell was greatly accelerated. This second stage has been observed in all cells except the lymphocyte, in which it may possibly occur to a slight degree. Finally there was the terminal stage, the stage of degeneration, in which the cell rounded up, lost its motility, and either became badly swollen or else underwent a more or less complete coagulation. 4. The factor causing this degeneration was found to be exposure of the culture to light, as, for example, during microscopic examination. 5. By a reduction of the infrared part of the spectrum, it was indicated that the effect was not due to a heat coagulation of the cells. 6. This degeneration was also found to occur in the complete absence of ultra-violet wave-lengths. 7. Further, it was shown that this degeneration was caused by light which lay within each of the three wave-length zones (1) 430µµ to 550µµ; infra-red; (2) 475µµ to 630µµ; 690µµ to infra-red; (3) 600µµ to infra-red. 8. No indication was given as to whether all regions of these zones were active in causing the degeneration, or whether the active rays are limited to certain wave-length bands lying within these zones. 9. This degeneration of the leucocytes under the action of light was also found to occur upon irradiation of hanging drops of whole blood. This is interpreted as showing conclusively that the degeneration was not dependent upon the additional factors of centrifugation, continued lowering of temperature, or the presence of abnormal saline solution. 10. It was noted, however, that the leucocytes in hanging drop cultures required a markedly longer time for their degeneration under the action of light than did the leucocytes in cultures prepared from the buffy coat and inoculated in serum. This is considered as possibly due, either to injury to the cell during centrifugation and subsequent handling, or to some action of the red blood cells present in large amounts in the hanging drops of whole blood. 11. In these hanging drop cultures of whole blood degeneration of the leucocytes was also found to occur when the light reaching the culture was first freed from the larger part of its infra-red and from all of its ultra-violet. 12. It was also shown that the same degeneration was produced by wave-lengths of light lying within each of the three wave-length zones defined in Section 6 of this summary.     

止痛活血化瘀药对血液流变性影响研究

本文了研究了止痛活血化瘀药对血液流变性的影响。两类止痛活血化瘀药在改善红细胞聚集性、变形性等血液流变性诸参数的作用是明显不同的。结果表明活血散瘀类药可明显改善四磺红细胞流变性参数，即通过调整红细胞表面电荷以增加细胞间的排斥力，防止红细胞聚集，改善变形性，降低纤维蛋白元含量，降低血液粘度，改善血液流变性，另一方面，祛风行气类药则可降低血小板聚集性和粘附性。因此，止痛活血化瘀药的作用机理是不同的。