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1.
Raf kinase inhibitory protein (RKIP, also PEBP1) is involved in regulation of multiple cellular signaling processes and suppressing
metastasis in animal models. Downregulation of RKIP expression has been shown to promote tumor progression in a variety of
human cancers. However, its role and clinical significance in resectable esophageal squamous cell carcinoma (ESCC) is still
scanty. The purpose of this study was to investigate the prognostic significance of RKIP expression by immunohistochemistry
in a group of patients with ESCC treated with surgical resection. RKIP expression in 233 surgically resected ESCC specimens
and 49 cases of adjacent normal tissues was detected by using immunohistochemical staining. The clinical and prognostic significance
of RKIP expression was statistically analyzed. Kaplan-Meier analysis was used to compare the postoperative survival between
groups. Significant downregulation was noted for RKIP protein in ESCCs, compared to adjacent normal tissues (p < 0.001). A lower disease-free survival and overall survival of ESCC was found in patients whose tissues had low RKIP expression
(both P < 0.001). In addition, RKIP expression could stratify the patient survival (disease-free survival/overall survival) in stage
II (P = 0.01 and 0.02, repectively). The Cox proportionate hazard regression model also established that low expression of RKIP
was significantly correlated with increased risk (RR = 3.572) of recurrence compared with high RKIP expression (P < 0.001). Furthermore, the results of multivariate analysis suggested that RKIP expression (P < 0.001) was an independent factor that affected overall survival. These findings suggest that the low expression of RKIP
be associated with poor survival in resectable ESCC patients. 相似文献
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3.
Savic A Cemerikic-Martinovic V Dovat S Rajic N Urosevic I Sekulic B Kvrgic V Popovic S 《Pathology oncology research : POR》2012,18(3):681-690
Angiogenesis has been implicated in the pathogenesis and prognosis of myelodysplastic syndrome (MDS). In this study, we investigated
the relationship between microvessel density (MVD), vascular endothelial growth factor (VEGF) expression, common morphological
and clinical factors, and survival in patients with MDS. We examined the MVD of paraffin-embedded bone marrow sections from
70 MDS patients and 31 controls. VEGF expression was determined in 50 patients and 20 controls. The median MVD in MDS patients
was significantly higher than that in controls (p = 0.025), whereas there was no difference in VEGF expression between MDS patients and controls. In univariate analysis, increased
MVD was associated with a shorter survival time (p = 0.023). However, in multivariate analysis, MVD was not an independent predictor of survival. The VEGF expression did not
influence survival in univariate analysis. Survival was independently influenced by platelet count (p = 0.0073), cytogenetic risk category (p = 0.022), and transfusion dependence (p = 0.0073). Neither MVD nor VEGF expression were predictors for progression to acute myeloid leukemia in univariate analysis.
Progression to acute myeloid leukemia was independently influenced only by the cytogenetic risk category (p = 0.022). This study confirmed increased MVD in MDS. It does not support an independent prognostic role of angiogenesis in
MDS. 相似文献
4.
5.
Sahar M. Hazzaa Osama M. Elashry Ibtesam K. Afifi 《Pathology oncology research : POR》2010,16(1):101-109
We investigated the feasibility of profiling and measuring the concentration of clusterin in urine and serum for individuals
with transitional cell carcinoma (TCC) of the bladder and comparing it with nontumor controls. In addition, we analyzed the
correlation of expression of clusterin in specimens of TCC to various clinicopathologic parameters and prognosis of bladder
cancer. Blood and urine samples were used from 68 patients with TCC of the bladder and from 61 patients with benign urological
diseases. Enzyme-linked immunosorbent assays (ELISA) were performed for clusterin from serum and urine. Quantitation of clusterin
mRNA was carried out in 68 bladder tumor specimens from radical cystectomy or transurethral resection and 26 normal bladder
specimens from BPH patients by using RT-PCR method. Correlation for the expression of clusterin mRNA with clinicopathologic
parameters was analyzed. Serum and urine clusterin was significantly higher in individuals with bladder cancer than control
(p = 0.001). Sensitivity and specificity of serum and urine clusterin as a tumor marker for TCC of the bladder was found to
be 80%, 91%, 87.1% and 96.7% respectively. Clusterin expression was significantly higher in TCC specimens than normal tissue
specimens (P < 0.001). Expression of clusterin was significantly higher in patients with invasive TCC of the bladder than that in patients
with superficial TCC and control (P < 0.001). Overexpression of clusterin mRNA was significantly associated with tumor recurrence and overall survival (p < 0.001). The recurrence-free survival time of patients with overexpression of clusterin was significantly shorter than that
of patients with weak expression of clusterin (9.8 months vs. 35.2 months). Clusterin may be considered as a potential diagnostic
and prognostic biomarker for bladder cancer using urine, serum and/or molecular biology techniques. 相似文献
6.
Bone morphogenetic protein (BMP) 4 plays a crucial role in tumor invasion and metastasis of various human cancers. However,
little is known about the correlation of BMP4 expression with clinical aggressiveness and prognosis in hepatocellular carcinoma
(HCC). The aim of this study was to investigate the expression of BMP4 in HCC and determine its correlation with tumor progression
and prognosis. Immunohistochemistry assay was used to determine the expression of BMP4 in HCC and corresponding paracarcinomatous
tissues from 156 patients. The potential prognostic value of BMP4 was investigated by comparing the survival rates between
the BMP4-positive and BMP4-negative HCC patients. Immunohistochemically, BMP4 protein expression in the HCC tissues (120/156,
76.9%) was significantly higher than that in the paracarcinomatous tissues (19/156, 12.2%, P < 0.01). The expression of BMP4
in HCC was associated with number of tumor nodules (P = 0.02), Edmondson grade (P = 0.03), TNM stage (P = 0.009), and vascular invasion (P = 0.006). In univariate survival analysis, the significant associations of the BMP4 protein overexpression with shortened
patients’ overall and disease-free survival were found (P = 0.001 and 0.006, respectively). Furthermore, its expression was found to be an independent factor for predicting both overall
(P = 0.009) and disease-free survival (P = 0.022) of HCC in multivariate analysis. Our data suggest for the first time that BMP4 is overexpressed in HCC tissues and
may also act as a novel marker for predicting the recurrence and prognosis of HCC patients after surgery. 相似文献
7.
The purpose of this study was to investigate the expression of Y-Box-binding protein 1 (YB-1) in breast cancer and its correlation
with clinicopathological characteristics and prognosis. Paraffin sections were retrospectively collected from 239 cases of
stage I–III breast cancer patients and 30 healthy females who received surgery between January 2000 and December 2004 in the
Chinese People’s Liberation Army General Hospital. The protein expression of YB-1 was detected by immunohistochemistry. The
expression difference between the two groups and the correlation between YB-1 expression and clinicopathological characteristics
and breast cancer prognosis were analyzed. Within the breast cancer group, YB-1 was expressed in the cytoplasm in 100.0% (239/239)
of cases and in the nucleus in 36.8% (88/239) of cases. Within the control group of normal breast tissue, YB-1 was expressed
in the cytoplasm in 100.0% (30/30) of cases and in the nucleus in 16.7% (5/30) of cases. The expression of YB-1 in the nucleus
of breast cancer cells was significantly higher than that in normal breast tissue (P = 0.029). The expression of YB-1 in the nucleus of breast cancer cells positively correlated with the Scarff–Bloom–Richardson
grade (P = 0.007) and HER-2 expression (P = 0.005), negatively correlated with ER expression (P = 0.004), and was independent of the age, menstrual status, pathological type, tumor size, lymph node status, presence of
thrombosis, PR expression, and EGFR expression. The 5-year disease-free survival (DFS) and overall survival (OS) of patients
with positive YB-1 expression in the nucleus were significantly lower than those of patients who were negative for nuclear
YB-1 expression, and the difference was statistically significant (DFS 65.9% vs. 82.1%, P = 0.000; OS 79.5% vs. 92.1%, P = 0.000). Multivariate analysis suggested that the expression of YB-1 in the nucleus is an independent prognostic factor
that affects DFS and OS in breast cancer patients (DFS P = 0.015; OS P = 0.035). In conclusion, the expression of YB-1 in the nucleus is related to carcinogenesis and the development of breast
cancer. Therefore, YB-1 is an important molecular marker that can be used to predict breast cancer prognosis. 相似文献
8.
Apostolos Zaravinos Maria Chatziioannou George I. Lambrou Ioannis Boulalas Dimitris Delakas Demetrios A. Spandidos 《Pathology oncology research : POR》2011,17(2):181-190
RKIP has been shown to regulate the RAS-RAF-MEK-ERK kinase cascade acting as modulator of apoptosis and metastasis in prostate
cancer. Our goal was to examine the expression of the RAF (A-RAF, B-RAF and RAF-1) and RKIP genes in urinary bladder cancer. Microarray analysis and qPCR was employed to investigate the expression of RAF and RKIP, in 30 patients with transitional cell carcinoma (TCC) of the urinary bladder vs. the corresponding levels of adjacent normal
tissue. Computational analysis was also performed on Gene Expression Omnibus (GEO) datasets, to unravel differences in the
expression of RAF or RKIP between tumor and control samples, and between superficial and muscle invasive tumors. Microarray analysis revealed >2-fold
expression of BRAF and RKIP in T2, T3, grade III tumors vs. controls. B-RAF over-expression was verified by qPCR in pT1, grade III tumors vs. their normal counterparts (p = 0.016). qPCR revealed a significant RKIP reduction in TCC vs. normal tissue (p = 0.002 and p < 0.001 for T1, grade II and Ta-T1, grade III, respectively); All RAF genes were positively correlated among each other (A-RAF/B-RAF, p = 0.003; A-RAF/RAF-1, p < 0.001; B-RAF/RAF-1, p = 0.050), whereas B-RAF was negatively correlated with RKIP in TCC (p = 0.050). Further computational analysis revealed different expression profiles for the genes of interest, among muscle invasive
carcinomas, superficial TCCs, cystectomy specimens and normal tissue. The reduced RKIP mRNA levels in TCC and the elevated levels of B-RAF in pT1, grade III tumors vs. normal tissue, corroborate that these genes are involved in the pathogenesis of urinary bladder
cancer. 相似文献
9.
Giaginis CT Vgenopoulou S Tsourouflis GS Politi EN Kouraklis GP Theocharis SE 《Pathology oncology research : POR》2009,15(2):173-181
Focal adhesion kinase (FAK), a non-receptor tyrosine kinase protein, acts as an early modulator of integrin signaling cascade,
regulating basic cellular functions. In transformed cells, unopposed FAK signaling has been considered to promote tumor growth,
progression and metastasis. The aim of this study was to assess the clinical significance of FAK expression in the two distinct
histological types of human gastric neoplasia. FAK expression was assessed immunohistochemically in tumoral samples of 66
gastric adenocarcinoma cases, 30 intestinal and 36 diffuse type, and was statistically analyzed in relation to various clinicopathological
characteristics, tumor proliferative capacity and patients’ survival. In intestinal type carcinomas, enhanced FAK expression
was significantly associated with increased tumor proliferative capacity (P = 0.012). In diffuse type carcinomas, FAK staining intensity was significantly correlated with tumor size (P = 0.026) and disease stage (P = 0.024), presenting also a borderline association with nodal status (P = 0.053). In diffuse type carcinomas, enhanced FAK expression was significantly associated with longer overall survival times
(log-rank test, P = 0.014), being also identified as an independent prognostic factor in multivariate analysis (Cox regression, P = 0.016). In contrast, patients with intestinal type tumors and enhanced FAK expression were characterized by shorter overall
survival times, without though reaching statistical significance (log-rank test, P = 0.092). The current data support evidence that FAK protein may be considered as a diagnostic and prognostic marker in gastric
neoplasia. Further studies conducted on larger clinical samples and highlighting on the distinct impact of the two histological
types are warranted to delineate the clinical significance of FAK protein in gastric neoplasia. 相似文献
10.
11.
Yan Shi Li Chen Jie Li Ya-Li Lv Qiong Sun Ling-Xiong Wang Shun-Chang Jiao 《Tumour biology》2011,32(2):381-390
Ras/ERK and PI3K/Akt pathways are reported to play a prognostic role and contribute to drug resistance in many cancers. The
objective of this study was to explore associations between the expression levels of several molecules in Ras/ERK and PI3K/Akt
pathways and their clinical significance in predicting the effectiveness of postoperative adjuvant chemotherapy in patients
with non-small cell lung cancer (NSCLC). The expressions of K-ras, Raf-1, ERK1/2, phosphorylated ERK1/2 (pERK1/2), Akt-1,
phosphorylated Akt-1 (pAkt-1), and Bcl-2 were detected by immunohistochemistry in tumor specimens from 144 NSCLC patients.
The correlations between the expression levels of these molecules and the clinicopathological characteristics were analyzed.
Patient survival was analyzed by Kaplan–Meier method, log-rank test, and Cox regression. The positive expression rates of
K-ras, Raf-1, ERK1/2, pERK1/2, Akt-1, pAkt-1, and Bcl-2 were 21.5%, 41.7%, 59.7%, 27.1%, 50.7%, 36.1%, and 30.6%, respectively.
Univariate analysis showed that patients with pERK1/2-positive (P = 0.01), Bcl-2-positive (P = 0.023), or pAkt-1 negative (P = 0.021) had significantly better recurrence-free survival (RFS) than those with pERK1/2-negative, Bcl-2-negative, or pAkt-1-positive.
Multivariate analysis showed that earlier stage (P ≤ 0.001), non-adenocarcinoma (P ≤ 0.001), pERK1/2-positive (P ≤ 0.001), and pAkt-1-negative (P = 0.016) were independent prognostic factors for a better RFS in NSCLC. pERK1/2-positive and pAkt-1-negative proved to contribute
to a better RFS in postoperative NSCLC patients who received adjuvant chemotherapy after taking the stage and histological
subtype into account. pERK1/2 and pAkt-1 could be considered as new independent prognostic biomarkers for predicting RFS and
selecting patients who are more likely to benefit from postoperative adjuvant chemotherapy. 相似文献
12.
Luo D Huang H Lu ML Zhao GF Chang J Zheng MY Wang Y 《Pathology oncology research : POR》2012,18(2):491-497
Although many molecular and biological studies have shown risk factors for gastric cancer, the available knowledge is still
insufficient to unveil the exact mechanism of gastric cancer. To investigate the relationships between Bves expression and
the clinicopathologic features of gastric cancer and whether Bves can act as prognostic indicators in gastric cancer. Tissues
were obtained from the gastrectomy specimens of 306 human gastric cancer and 78 noncancerous gastric tissue at the Department
of Surgery and Pathology, the Second Affiliated Hospital of Kunming Medical University from February 1996 to March 2007. The
method of immunohistochemistry was used to investigate the expression of Bves in them. The relationship between Bves expression
and the survival times of the patients was retrospectively analysed. Reduced expression of Bves frequently occurred in gastric
cancer tissue. Low expression of Bves correlated with histologic differentiation, depth of invasion, regional lymph nodes
and distant metastasis, and TNM stages (P < 0.05). Bves expression did not correlate with age, gender, location of tumor, size of tumor and histologic type (P > 0.05); Further multivariate analysis revealed that lymph node metastasis (P < 0.0001), distant metastasis (P < 0.0001), surgical treatment (P < 0.0001), and the expression of Bves (P < 0.0001) were independent prognostic factors in patients with gastric cancer; The Kaplan-Meier plot showed that survival
times of patients with low Bves expression was significantly lower than those in patients with high Bves expression. Besides,
low Bves expression had a much more significant effect on the survival of those patients with early stage tumors (χ2 = 131.216,P < 0.0001), highlighted by a >51.3% reduction in 3-year survival compared with that of patients with high Bves expression.
In late stages, the difference was also significant (χ2 = 5.818,P = 0.016), with a 34.8% reduction in 3-year survival. Reduced expression of Bves in gastric cancer is associated with tumor
progression and the patient’s poor survival. This study showed that the studied protein has further provided a basis for the
development of potential biomarker for gastric cancer prognosis. 相似文献
13.
14.
This study aims to investigate the expression and significance of glucose-6-phosphate dehydrogenase (G6PD) in human gastric
cancer progression and prognosis. Using immunohistochemistry and real-time RT-PCR assay, we identified abnormally elevated
expression of G6PD in gastric cancer tissues compared to paired normal stomach mucosa tissues in 24 patients (p < 0.05). In order to investigate the correlations between G6PD and the clinicopathological features of gastric cancer, the
expression of G6PD in 167 patients with gastric cancer were detected by immunohistochemistry, and the results showed that
overexpression of G6PD was associated with the size of tumor (p = 0.039), depth of invasion (p = 0.039), lymph node metastasis (p = 0.044), distant metastasis (p = 0.003), TNM stage (p = 0.030), and survival rate (p = 0.010). Further, Cox multivariates analysis indicated that G6PD expression level was an independent prognostic factor for
patients after radical resection (p = 0.013). In conclusion, overexpression of G6PD is closely related to progression of gastric cancer, and might be regarded
as an independent predictor of poor prognosis for gastric cancer. 相似文献
15.
The aim of this study was to identify potential epigenetic prognostic biomarkers for colorectal cancer (CRC) in the Chinese
population. The methylation status of five tumor suppressor genes (CDH13, DLEC1, FBLN3, hMHL1 and RUNX3) was determined using manual microdissection followed by methylation-specific PCR in 85 paired CRC specimens and adjacent
normal tissue. The results showed that methylation frequencies in cancerous tissues were 31.8% for CDH13, 37.6% for DLEC1, 38.8% for FBLN3, 22.4% for hMHL1 and 27.1% for RUNX3, all of which were significantly higher than in corresponding normal tissue. Furthermore, CDH13 methylation was associated with poor differentiation (P = 0.019) and tended to be predominant in advanced stages (P = 0.084); FBLN3 methylation was associated with advanced stages (P = 0.027) and lymph node metastasis (P = 0.029). Accordingly, the methylation status of CDH13 (P = 0.022), FBLN3 (P = 0.008), CDH13 and/or FBLN3 (P = 0.001) predicted adverse overall survival in CRC, while hMHL1 methylation showed a protective role in survival (P = 0.046). Cox proportional hazard models further indicated that CDH13 and/or FBLN3 methylation, but not that of hMHL1, was an independent prognostic factor for CRC. In conclusion, we found CDH13 and FBLN3 gene methylation are potential biomarkers for poor prognosis in CRC. 相似文献
16.
Wilms tumor is a mixed embroynal neoplasm of the kidney . HER2 is an onco-protein. Its over-expression could be implicated
in the development of many tumors. The clinico-demographic and pathological data of 28 Wilms tumor patients were , reviewed.
The tissue samples were examined by light Microscopy then immunohistochemical staining for HER2/neu expression. Additional
28 normal surrounding renal tissue specimens were included. There was significant differences between HER2/neu positive and
HER2/neu negative Wilms tumors in relation to stage, histological phase and epithelial differentiation (P > 0.05 for all). The overall survival advantage was noticed if Wilms tumor was at early stages (I and II) (Log-rank = 13.23
and P > 0.001), homologous epithelial differentiation (Log-rank = 6.01 and P = 0.04), as well as HER2/neu positive tumors (Log-rank = 6.14 and P = 0.013). A statistical significant trend toward a longer recurrence free survival was, noticed if Wilms tumor was at early
stages (Log-rank = 21.22, P > 0.0000) and if HER2/neu positive (Log-rank = 8.53, P = 0.004). HER2/neu expression in Wilms tumor could be a marker for epithelial and homologous differentiation and its expression
could be a good predictor for overall survival and longer recurrence free survival. 相似文献
17.
Lyronis ID Baritaki S Bizakis I Krambovitis E Spandidos DA 《Pathology oncology research : POR》2008,14(3):267-273
The Ras/Raf/MEK/ERK (MAPK) signal transduction cascade is an important mediator of a number of cellular fates including growth,
survival and apoptosis. The aim of this study was to determine the incidence of B-raf, Kirsten-ras (K-ras) and Neuroblastoma-ras
(N-ras) gene mutations in esophageal squamous cell carcinoma (ESCC) in the Greek population. DNA was extracted from 30 ESCC
and 32 normal esophageal specimens and screened for V600E B-raf, and K-ras/N-ras codon 12 mutations, by PCR-RFLP based analysis.
Among the genes tested, only the heterozygous K-ras mutation was detected in 5 out of the 30 ESCC specimens (16%), whereas
no mutation was found in the normal esophageal tissue (P < 0.022). The normal samples were screened negative for N-ras and V600E B-raf mutations. The increased risk of esophageal
cancer was correlated with tobacco use (OR = 3.5, P < 0.023) and alcohol abuse (OR = 7.22, P < 0.001), accompanied with the high incidence of the k-ras codon 12 mutation (22%, OR = 1.77 and 21%, OR = 1.52), respectively.
A similar positive association was seen in human papilloma virus (HPV)-infected patients (OR = 5.66, P < 0.003). Our overall findings demonstrate that the mutational activation of the K-ras gene, HPV infection and tobacco or
alcohol abuse, can be considered independently or in combination as high risk factors for ESCC development. 相似文献
18.
Miao-zhen Qiu Bing Han Hui-yan Luo Zhi-wei Zhou Zhi-qiang Wang Feng-hua Wang Yu-hong Li Rui-hua Xu 《Tumour biology》2011,32(1):159-166
The impact of hypoxia-inducible factor (HIF)-1α and hexokinase-II (HK-II) expression on prognosis of gastric adenocarcinoma
patients has not been clearly established. We identified all patients in Cancer Center of Sun Yat-Sen University who were
diagnosed as gastric adenocarcinoma and underwent radical gastrectomy between January 1999 and December 2001. We used immunohistochemistry
to determine the expressions of HIF-1α protein and HK-II in the surgical sections. We identified 188 patients with gastric
adenocarcinoma for the final analysis. The positive rate of HIF-1α and HK-II were 110/188 (54.6%) and 40/188 (21.3%), respectively.
Both HIF-1α and HK-II were all positively correlated with tumor size, lower differentiation, and tumor stage. Univariate analysis
showed that advanced tumor stages (P < 0.001), tumor size (P = 0.003), HIF-1α expression (P < 0.001), and HK-II expression (P < 0.001) were all significantly associated with shorter survival. The multivariate Cox analysis revealed that tumor stage
(P < 0.001), HIF-1α expression (P < 0.001), and HK-II expression (P = 0.002) remained independent prognostic variables for survival. In addition, there was a positive correlation of HIF-1α
protein expression and HK-II (P = 0.022). Both HIF-1α and HK-II were overexpressed in gastric adenocarcinoma. The multivariate Cox analysis revealed that
both of them were independent factors on survival of gastric adenocarcinoma patients. 相似文献
19.
Yijun Xu Mingchen Zhu Shuhong Zhang Hui Liu Tao Li Chengyong Qin 《Pathology oncology research : POR》2010,16(2):169-175
Phosphatase of regenerating liver (PRL)-3 is involved in the metastasis of various tumors, but the expression of PRL-3 and
its possible role in primary intrahepatic cholangiocarcinoma (ICC) has not been reported yet. In this study, we assessed the
expression levels of PRL-3 by immunohistochemistry in 102 primary ICC samples, 62 matched lymph node metastases (LNM) and
102 adjacent normal liver tissues. Then we investigated the relationship between PRL-3 expression and clinicopathologic factors.
Survival analysis was performed to determine the prognostic significance of PRL-3 expression in ICC. Immunochemistry results
suggested PRL-3 expression was negative or weak in non-neoplastic intrahepatic bile ducts of adjacent liver tissue. In primary
lesion and LNM high PRL-3 expression was frequently detected. Furthermore, the rate of high PRL-3 expression in LNM was higher
than that in primary lesion (80.6% vs. 47.1%, P < 0.05). High expression of PRL-3 in primary tumors was significantly associated with TNM (P < 0.001), T stage (P < 0.001), vascular invasion (P = 0.002), and LNM (P < 0.001). Survival analysis results with Kaplan-Meier method and Cox proportional hazard model indicated high expression of
PRL-3 was correlated with decreased overall survival and was an independent prognostic marker of overall survival. Thus, our
results suggested high expression of PRL-3 was correlated with progression and metastasis of ICC and indicated negative prognostic
impact. PRL-3 might serve as a novel prognostic marker for patients with ICC. 相似文献
20.
Lakatos G Sipos F Miheller P Hritz I Varga MZ Juhász M Molnár B Tulassay Z Herszényi L 《Pathology oncology research : POR》2012,18(1):85-91
Matrix metalloproteinases play an important role in extracellular matrix remodelling. It has been proposed that matrix metalloproteinase-9
(MMP-9) is involved in epithelial damage in ulcerative colitis (UC). However, to our knowledge, no data are available in terms
of MMP-9 expression in microscopic colitis. Determination of mucosal protein expression levels of MMP-9 in lymphocytic colitis
(LC), collagenous colitis (CC) and UC. MMP-9 immunohistochemical expressions were analyzed in paraffin-embedded tissue samples
by immunohistochemistry including patients with LC, CC, UC, active diverticulitis, inactive diverticular disease and healthy
control subjects. UC was also subgrouped according to the severity of inflammation. Immunostaining was determined semiquantitatively.
Independent colonic biopsies from healthy and severe UC cases were used for gene expression analyses. For further comparison
MMP-9 serum antigen levels were also determined in patients with UC and control patients without macroscopic or microscopic
changes during colonoscopy. MMP-9 mucosal expression was significantly higher in UC (26.7 ± 19.5%) compared to LC (6.6 ± 9.3%),
CC (6.4 ± 7.6%), active diverticulitis (5.33 ± 2.4%), inactive diverticular disease (5.0 ± 2.2%) and controls (6.3 ± 2.6%)
(P < 0.001). The immunohistochemical expression of MMP-9 in LC and CC was similar as compared to controls. MMP-9 expression
was significantly higher in each inflammatory group of UC compared to controls (mild: 11.0 ± 2.8%, moderate: 23.9 ± 3.7%,
severe UC: 52.6 ± 3.9% and 6.3 ± 2.6%, respectively, P < 0.005). The gene expression microarray data and RT-PCR results demonstrated a significantly higher expression of MMP-9
in severely active UC compared to healthy controls (P < 0.001). Significantly higher MMP-9 serum antigen concentrations were observed in UC patients compared with the control
group (P < 0.05). MMP-9 seems to play no role in the inflammatory process of LC and CC. In contrast, the mucosal up-regulation of
MMP-9 correlated with the severity of inflammation in UC. The increased MMP-9 expression could contribute to the severity
of mucosal damage in active UC. 相似文献