氟化物涂膜对正畸固定矫治中牙釉质脱矿影响的SEM研究

目的：采用扫描电镜（SEM）观察氟化物涂膜对抑制离体牙牙釉质脱矿及促进再矿化的功效。方法：选择因正畸需要拔除的前磨牙15颗,经37％磷酸处理,制备人工釉质脱矿标本,并用氟化物涂膜进行表面处理。采用SEM对标本脱矿前（对照组）,脱矿后（脱矿组）以及氟化物涂膜治疗后（治疗组）的表面形貌进行观察分析。结果：对照组与脱矿组之间、脱矿组与治疗组之间形态学存在显著差异,而对照组与治疗组之间形态学差异相对较小。结论：氟化物涂膜可有效改善釉质脱矿后的牙齿脱矿形貌。     

光学相干断层成像检测牙釉质表层脱矿与再矿化的实验研究

目的利用光学相干断层成像技术(OCT)观察不同制剂对年轻恒牙釉脱矿和再矿化效果的影响。方法 将正畸拔除的年轻恒前磨牙制成60个釉质块,随机均分为4组。每天进行如下处理:A组蒸馏水湿敷30min;B组含氟牙膏湿敷30min;C组护牙素湿敷30min;D组多乐氟湿敷30min,置于脱矿液1.5h,最后置于人工唾液中22h。每个步骤间用去离子水冲洗实验样品。实验周期重复30天。在处理前和第 7、15、30天,使用扫频光源OCT完成对样本的扫描和成像,同时使用显微硬度计测量各样本表面的显微硬度。结果 釉质脱矿深度:7天后,A组与其他各组相互比较,差异有统计学意义( P <0.05),B、C、D组组间比较差异无统计学意义( P >0.05)。30天后,C组和D组比较,差异无统计学意义( P >0.05),其余各组之间两两比较,差异均有统计学意义( P <0.05)。显微硬度测量:30天后,各处理组较处理前显微硬度值降低,A组显微硬度值最低,C组与D组显微硬度值高于B组,且差异有统计学意义( P <0.05)。C组和D组间釉质块显微硬度值差异无统计学意义( P >0.05)。结论 OCT可以较准确的显示釉质脱矿部位,在早期龋的诊断方面具有临床意义。护牙素和多乐氟均能有效的抑制牙釉质表面脱矿。     

Effect of sodium fluoride on oral biofilm microbiota and enamel demineralization

ObjectiveFluoride is widely used as an anti-caries agent, e.g. in toothpastes and mouth rinses. However, the nature of the anti-caries action is not entirely clear. Mechanisms suspected to explain the cariostatic effect include inhibitory effects on acid formation by bacteria, inhibition of extracellular polysaccharide (EPS) production, inhibition of enamel demineralization and enhancement of remineralizaton or combination thereof. The aim of this study was to examine with the supragingival Zurich in vitro biofilm model the effect of fluoride in NaF formulation, on the microbiota and on demineralization.MethodsBiofilms consisting of Actinomyces oris, Candida albicans, Fusobacterium nucleatum, Streptococcus oralis, Veillonella dispar and Streptococcus sobrinus, were grown anaerobically on sintered hydroxyapatite or bovine enamel disks, exposed to 200, 400, and 1400 ppm of NaF, or 0.1% chlorhexidine (positive control). The biofilms were harvested after 64 h and CFUs were assessed for total bacteria. Demineralization of enamel disks was measured by quantitative light-induced fluorescence.ResultsNaF did not affect the bacterial numbers. No enamel mineral loss was observed at 1400 and 400 ppm of fluoride, whereas the pH of the surrounding medium was increased to 5.5 and 5.0, respectively, compared to the untreated control (pH 4.5 and mineral loss ΔF of −32%). At 1400 ppm NaF the biofilm’s EPS volume was also significantly reduced.ConclusionsAdministration of NaF completely prevented demineralization without affecting biofilm composition and growth. This protective effect may be attributed to the observed decrease in acid production or EPS volume, or to a shift in the de/remineralization balance.     

Effect of low level fluoride on demineralization kinetics of human dental enamel

The purpose of this study was to examine the effect of fluoride levels similar to those reported for saliva from low fluoridated and high fluoridated water areas on the demineralization of human permanent enamel. An adaptation of the method described by Robinson et al. was used. Sections of sound enamel were immersed in a vial containing demineralizing solution [2.0 mM Ca(NO₃)₂, 1.2 mM KHPO₄ in 50 mM acetic acid, pH 4.8] for 1 hour. The demineralizing solution contained 0, 0.02 or 0.05 ppm fluoride, added as NaF was prepared. Twenty microliters aliquots were taken from the demineralizing solution at the time point up to 1 hour, with a sampling frequency bias towards the early time point. The phosphate content of the removed sample was determined by colorimetry. When the mineral loss curves for fluoridated and non-fluoridated demineralizing solutions were compared, there were significant differences between both groups. There was a decrease in the net mineral loss when fluoride was used. This result suggested that salivary fluoride levels of 0.02 ppm and 0.05 ppm had a protective effect against demineralization.     

Efficacy of CPP-ACP fluoride varnish applied with and without acid etching in preventing enamel demineralization compared to light-curable fluoride varnish

ObjectivesTo compare efficacy of casein phosphopeptide (CPP)–amorphous calcium phosphate (ACP) fluoride varnish and light-curable resin modified glass ionomer fluoride varnish (FV) in preventing white spot lesions and evaluating acid etching prior to CPP-ACPFV application on its efficacy.Materials and MethodsMolars and premolars were transected and halves divided into four groups (n = 18/group): (1) resin-modified glass ionomer FV: etched and Clinpro-XT varnish (3M ESPE, Pymble, New South Wales, Australia) application; (2) CPP-ACPFV: MI varnish (GC America, Alsip, IL) application; (3) Etch+CPP-ACPFV: etched and MI varnish application; (4) Control: etched and no surface treatment. To simulate 12 weeks in an intraoral environment, samples were subjected to thermocycling, brushing, and pH cycling. Enamel surface microhardness was evaluated at baseline and after the simulated 12 weeks. Representative samples were also assessed using scanning electron microscopy (SEM).ResultsAt baseline there was no significant difference in microhardness among groups. After the simulated 12 weeks, all groups showed significant within-group differences (P < .001). Control showed the highest percentage loss of surface microhardness (89%), followed by CPP-ACPFV (58%), RMGIFV (51%), and Etch+CPP-ACPFV (24%). The control group had a significant decrease in microhardness compared to all experimental groups (P < .001). No difference was found between the RMGIFV and CPP-ACPFV varnish groups. The Etch+CPP-ACPFV group had significantly less decrease in microhardness compared to the RMGIFV (P < .001) and CPP-ACPFV groups (P < .001). With SEM, control samples showed signs of enamel surface damage, while experimental groups showed spherical particles on a relatively intact surface.ConclusionsRMGIFV and CPP-ACPFV are effective in reducing enamel demineralization. Acid etching the enamel surface prior to CPP-ACPFV varnish application increased its efficacy.     

Comparison of the remineralization potential of CPP-ACP and CPP-ACP with 900 ppm fluoride on eroded human enamel: An in situ study

Objective

The aim of this in situ study was to compare the remineralization potential of pastes containing CPP-ACP and CPP-ACP with 900 ppm fluoride on human enamel softened by a cola drink.

Design

Forty-five enamel specimens obtained from human third molar teeth were eroded in a cola drink for 8 min and then attached to intra-oral devices worn by five volunteers. The specimens were subjected to three different in situ remineralization protocols using: (1) CPP-ACP (Group I), (2) CPP-ACP with 900 ppm fluoride (Group II), and (3) saliva (Group III, control). Vickers microhardness measurements were obtained at baseline followed by demineralization and remineralization stages.

Results

The CPP-ACP, CPP-ACP with 900 ppm fluoride and saliva controls resulted in 46.24%, 64.25% and 2.98% increase in post-erosion microhardness values, respectively. One-way ANOVA revealed statistically significant differences in the mean microhardness values between pastes containing CPP-ACP and CPP-ACP with 900 ppm fluoride.

Conclusions

Both CPP-ACP and CPP-ACP with 900 ppm fluoride substantially remineralized the softened enamel, with the CPP-ACP and fluoride combination showing higher remineralization potential than CPP-ACP. This study confirmed the synergistic effect of fluoride with CPP-ACP on remineralization of eroded enamel.     

Effect of silver and fluoride ions on enamel demineralization: a quantitative study using micro-computed tomography

Background: This study aimed to investigate the effect of silver and fluoride ions on demineralization of enamel. Methods: The coronal parts of 40 extracted sound premolars were prepared into tooth blocks. An unvarnished occlusal surface window (OW) and a flat buccal/lingual surface window (FW) were created for each tooth by covering all other surfaces with an acid‐resistant varnish. These blocks were randomly allocated into four groups of 10 blocks each and immersed in respective solutions for 5 minutes: Group 1 – 2.36 M silver fluoride; Group 2 – 2.36 M potassium fluoride; Group 3 – 2.36 M silver nitrate; and Group 4 – deionized water. After 7‐day immersion in a buffered demineralization solution at pH 4.4, micro‐CT scans were taken. Results: Mean lesion depth in the FW area for tooth blocks in AgF, KF, AgNO₃ and control groups were 0 μm, 3.3 ± 10.3 μm, 156.3 ± 30.8 μm, and 173.6 ± 48.6 μm, respectively (p < 0.001). The difference in mean lesion depth between the AgNO₃ and control groups was not statistically significant (p > 0.05). Similar OW and FW lesions were observed in tooth blocks in the AgNO₃ and control groups. Conclusions: Topical application of a 2.36 M fluoride solution can inhibit demineralization of enamel while topical application of silver ions has little effect.     

Effects of Galla chinensis on inhibition of demineralization of regular bovine enamel or enamel disposed of organic matrix

Objective

This in vitro study was undertaken to assess the effects of Galla chinensis extract on inhibition of enamel caries-like demineralization and to elucidate the role of the organic matrix of enamel in this process.

Design

Either regular or enamel disposed of its organic matrix both of bovine origin were exposed to a demineralizing solution for 3 days (pH 4.5). Specimes were additionally treated with either 4 g/L of G. chinensis extraction (GCE) or double distilled water (DDW) four times daily for 5 min each time. Regular enamel exposed accordingly to sodium fluoride (1 g/L) during the demineralizing period served as positive control. After exposure mineral loss and lesion depth of all samples were analysed by transversal microradiography. One-way ANOVA and Student-Newman-Keuls test were used to compare the differences amongst groups. A factorial ANOVA was chosen to test the interaction between GCE and enamel organic matrix.

Results

Mineral loss and lesion depth of specimens in the positive control group were significantly lower compared to all other groups. Regular enamel treated with GCE showed significantly lower values compared to regular enamel treated with DDW or to enamel disposed of its organic matrix (p < 0.05). These three groups revealed similar values (p > 0.05). Significant interaction between GCE and enamel organic matrix with respect to both outcomes could be observed (p < 0.05).

Conclusions

G. chinensis inhibits enamel caries-like demineralization in vitro. However, its potential seems to be weaker compared to sodium fluoride. The organic matrix of enamel was shown to play a substantial role in the observed mechanism.     

Effects of fluoride concentration on enamel demineralization kinetics in vitro

Objectives

The aim of the present study was to measure the effects of fluoride concentration on the real-time in vitro demineralization of enamel during exposure to caries-simulating conditions using Scanning Microradiography (SMR).

Methods

Enamel blocks obtained from non-carious human molars were fixed in SMR environmental cells, through which acidic solutions (0.1 M acetic acid, pH 4.0) were circulated for periods of 48 h. SMR was used to quantitatively measure continuous mineral mass loss. Subsequently, the effects of sequentially increasing fluoride concentration (0.1–4500 mg/L [F⁻]) in the acidic solutions were measured on the rate of enamel demineralization.

Results

The data shows a log-linear relationship between [F⁻] and reduction in demineralization up to 135 mg/L [F⁻]. Above 135 mg/L, no further significant decrease in demineralization occurred.

Conclusion

The optimum range of local fluoride concentration for reducing enamel demineralization was in the range 0.1–135 mg/L [F⁻] under the conditions studied.

Clinical significance

Relatively low [F⁻] can exhibit near-optimum protection. Increasing the fluoride concentrations above 135 mg/L may not necessarily give an increased cariostatic benefit. Improving the means of delivery of relatively low fluoride concentrations to the oral fluids through slow releasing mechanisms, such as the oral fluoride reservoirs, is the more appropriate way forward for sustaining long-term clinical efficacy.     

Effect of xylitol:sorbitol on fluoride enamel demineralization reduction in situ

OBJECTIVES: To evaluate if sugar alcohols would reduce enamel demineralization enhancing the fluoride (F) effect. METHODS: A crossover in situ study was conducted in four phases, during which 10 volunteers were submitted to one of the treatments: (I) Distilled and deionized water, as a negative control; (II) F (226 microg F/ml as NaF; concentration used in commercial mouthrinse); (III) X:S (xylitol:sorbitol 1:3; final concentration 1.6M; 28% of sugar alcohols) and (IV) F+X:S (same final concentration that groups II and III). The volunteers wore palatal appliances containing four bovine enamel blocks of known surface microhardness (SMH), covered with a 'test plaque' of mutans streptococci, which were immersed during 1 min in one of the allocated treatment solutions simultaneously that the volunteers rinsed their mouths with the same solution. After the rinsing the appliances were put in the mouth and after 20 min a cariogenic challenge was made with 20% sucrose solution during 1 min. After further 45 min the 'test plaque' was collected for F analysis, enamel SMH was again determined and the percentage of change in relation to baseline was calculated; F uptake in enamel was also determined. RESULTS: With respect to all the analyses made, the group F+X:S did not differ from the F treatment (p>0.05) and the groups treated with F and F+X:S differed from the negative control (p<0.05). CONCLUSIONS: The results suggest that xylitol:sorbitol may not enhance the effect of fluoride present in mouth rinse on the reduction of enamel demineralization.     

釉质脱矿与再矿化的实验室及临床评估方法

釉质再矿化的研究是近年研究的热点,对釉质再矿化前后的评估方法多种多样,目前国际尚无统一的标准。作者就评估脱矿与再矿化的实验室及临床研究方法及其应用范围、优缺点进行综述。     

Fluor Protector抑制饮料致牛牙釉质脱矿的实验研究

目的 :研究FluorProtector对饮料导致牛牙釉质脱矿的抑制作用。方法 :将 40个牛牙标本分成实验组和对照组 ,每组又分为 2个亚组。实验组在FluorProtector预处理釉质后用不同种类饮料处理 ,对照组未经FluorProtector预处理而直接用饮料处理。饮料的处理是 5min/次 ,5次 /d ,共处理 7d。采用微量化学分析法测定饮料在处理牙釉质后 1～ 7d饮料中钙和磷浓度的变化。结果 :实验组与对照组经同种饮料处理后钙、磷溶出的浓度明显不同 ,表现为实验组低于对照组 ,差异具有显著性 (P <0 .0 5 )。结论 :FluorProtector对饮料所致的牙釉质脱矿具有明显的抑制作用 ,并随时间的延长而减弱。     

氟保护漆对青少年固定正畸中釉质脱矿的影响

目的 探讨氟保护漆对青少年正畸患者固定矫治后釉质脱矿发生率的影响。方法 将80例16岁以下青少年固定正畸矫治患者随机分成2组：对照组40例（721颗）,对患者及家长进行口腔健康教育,不涂氟;实验组40例（742颗）,除了对患者和家长宣教外,戴用固定矫治器时及正畸期间每6个月用氟保护漆处理牙面。临床观察2年,对粘接托槽的牙齿观察其脱矿程度。结果 实验组中706颗牙脱矿指数为0,出现脱矿的有36颗,脱矿率为4.85%,未出现脱矿指数大于1的患牙。对照组中有604颗牙脱矿指数为0,出现脱矿的有117颗,脱矿率为16.23%,其中脱矿指数大于1的患牙有18颗。两组间差异有统计学意义（P<0.05）。结论 固定正畸患者戴用固定矫治器时及矫治期间使用氟保护漆能有效预防正畸牙釉质脱矿。     

Effect of fluoride containing bleaching agents on enamel surface properties

OBJECTIVES: To evaluate the effects of fluoridated bleaching agents and post-bleaching fluoridation treatment on the whitening efficiency and microhardness of bovine enamel. METHODS: Twenty five freshly extracted bovine incisors were cut into halves, embedded and then divided into the following five groups: Group 1, untreated controls; Group 2, treatment with 10% carbamide peroxide (CP) bleaching agent; Group 3, treatment with 10% CP followed by a 0.9% sodium fluoride gel application, Group 4, treatment with 10% CP containing 0.11% fluoride; Group 5, treatment with an experimental bleaching agent consisting of 10% CP and 0.37% fluoride. Groups 2-5 were treated 8h per day for 14 days then immersed in saliva for 2 weeks. Enamel morphology changes were evaluated under SEM on Day 14. Changes in enamel color and microhardness were evaluated on Days 7 and 14, and compared with the baseline data. Additionally, microhardness was determined on post-bleaching Days 21 and 28. RESULTS: After 2 weeks, an erosion pattern was noted on the specimens in Groups 2 and 3. Groups 4 and 5 showed a milder demineralized pattern. All the bleached enamel specimens revealed increased whiteness and overall color value. Groups 2 and 3 showed significantly decreased enamel microhardness compared to their baseline data. The specimens treated with fluoridated bleaching agents showed relatively less reduction in enamel microhardness than those treated with nonfluoridated agents during the bleaching treatment. CONCLUSIONS: The fluoridated bleaching agents produced less demineralization of surface morphology and microhardness. The addition of fluoride did not impede the whitening effect.     

含氟牙膏对固定矫治中牙釉质脱矿预防效果的研究

目的：探讨含氟牙膏在正畸固定矫治中对预防牙釉质脱矿的作用。方法本实验通过常规口腔卫生宣教指导接受固定矫治器治疗的患者分别使用不同种牙膏刷牙,将患者随机分为氟化物组和对照组,氟化物组采用含氟牙膏刷牙,对照组采用不含氟牙膏刷牙,应用时间平均18个月。分别比较矫治前后两组患者及被观察牙齿牙釉质脱矿发病率和脱矿程度,判断含氟牙膏在固定矫治中对牙釉质脱矿的预防效果。结果在正畸固定矫治中,应用氟浓度为0．15%的含氟牙膏刷牙,可明显降低牙釉质脱矿的发病率,明显减少牙釉质3度脱矿的发生。结论0．15%含氟牙膏应用于固定矫治能够降低牙釉质脱矿的程度。     

两种氟防龋涂膜材料的释氟性及抗脱矿能力的研究

目的 比较两种氟防龋涂膜材料的释氟性及其提高釉质的抗脱矿能力。方法 将两种市售的FQ氟涂膜和多乐氟涂膜分别涂覆于20 mm×20 mm的方形聚酯塑料载片表面，每个聚酯塑料载片浸于20mL去离子水中，分别于浸水后3 h、6 h、12 h及24 h测定氟离子浓度，计算释氟率；扫描电镜观察氟涂膜表面形貌。制备5 mm×5 mm×3 mm的牛切牙釉质块，自凝树脂包埋后表面打磨抛光，测定初始表面显微硬度(SMH0)，在釉质块表面涂覆氟涂膜，浸水12 h，然后去除氟涂膜，对釉质块进行pH循环脱矿，7天后测定釉质块表面显微硬度(SMH1)，计算脱矿后釉质表面显微硬度下降率，设立阳性对照和阴性对照。结果 FQ氟涂膜和多乐氟3 h、6 h、12 h、24 h释氟率分别为(2.73±0.27)%、(3.19±0.05)%、(3.58±0.19)%、(3.63±0.08)%和(0.16±0.02)%、(0.34±0.03)%、(0.43±0.03)%、(0.48±0.03)%；经过pH循环脱矿后，应用过氟涂膜的釉质表面显微硬度下降率显著低于未用氟涂膜的釉质（85.95±3.50）%,FQ氟涂膜和多乐氟的硬度...     

Effect of application sequence of fluoride and CPP-ACP on remineralization of white spot lesions in primary teeth: An in-vitro study

ObjectiveTo explore how application sequence of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and fluoride influences remineralization of enamel white spot lesions (WSL) in primary teeth.DesignIn this in-vitro study, artificial WSLs were created in 130 primary teeth. Teeth were divided into 4 groups (n = 27) and a control group (n = 22) and exposed to one of the following remineralization regimens for 10 weeks: Group-1; 500 ppm fluoride dentifrice; Group-2; 10% w/v CPP-ACP; Group-3; fluoride applied first, then CPP-ACP; Group-4; CPP-ACP applied first, then fluoride, and Group-5 was control. All groups were kept in a remineralizing solution. Mineral changes (ΔF) were quantified weekly using quantitative light-induced fluorescence. Statistical analysis was done using Statistical Package for the Social Sciences (SPSS version 20.0).ResultsRemineralization occurred in all groups to different degrees; changes from baseline were significant in groups 1–4 (P ≤ 0.05). Group-4 showed the earliest significant remineralization (after 2 weeks) among groups, (P < 0.001). Group-4 showed maximum changes in ΔF among groups; however, only differences with Groups 1 and 5 were significant (P < 0.05 and P < 0.01, respectively). Group-3 showed better remineralization than Groups 1, 2 and 5; however, the difference was only significant with Group-5 (P < 0.001). There were no significant differences between Group 1and 2, however, only Group 2 showed better remineralization than Group 5, (P < 0.01).ConclusionCombined treatment with CPP-ACP followed by fluoride exhibited the best remineralization of white spot lesions in primary teeth in this study. Combined treatment with fluoride followed by CPP-ACP showed a tendency towards better remineralization than fluoride or CPP-ACP alone.     

Effect of fluoride varnish supplemented with sodium trimetaphosphate on enamel erosion and abrasion: An in situ/ex vivo study

Objective

To evaluate the effect of fluoride (F) varnishes supplemented or not with sodium trimetaphosphate (TMP) on enamel erosive wear followed or not by abrasion in situ.

Methods

Ten volunteers were selected and randomly divided into four groups, according to the varnishes tested: placebo (no F or TMP), 5% NaF (positive control), 2.5% NaF and 2.5% NaF/5% TMP. Enamel blocks (n = 4) were mounted in palatal devices and received an application of each test varnish, following a double-blind, crossover protocol. After 6 h, varnishes were completely removed and the blocks were subjected to erosive challenges by ex vivo immersion in citric acid (5 min, 4×/dia, 5 days). Following, half of the blocks were subjected to abrasion by brushing with a placebo dentifrice slurry for 15 s. Enamel wear (μm), surface hardness (SH_f) and cross-sectional hardness (ΔKHN) were assessed after each experimental period. Results were analyzed by ANOVA, Student–Newman–Keuls's test and Pearson correlation coefficient (p < 0.05).

Results

The fluoride varnish supplemented with TMP promoted significantly lower wear and ΔKHN when compared to the other groups after erosive challenges, followed or not by abrasion (p < 0.05). As for (SH_f) the fluoride varnish supplemented with TMP promoted similar results to the 5% NaF product, being significantly higher than the remaining groups after erosive and erosive + abrasive challenges (p < 0.05).

Conclusion

TMP significantly enhanced the effects of F on enamel wear after erosive challenges, followed or not by abrasion.     

Effect of added calcium phosphate on enamel remineralization by fluoride in a randomized controlled in situ trial

Dental products containing calcium phosphate and fluoride are claimed to enhance enamel remineralization over fluoride products.

Objectives

To compare remineralization of enamel subsurface lesions by dental products with added calcium phosphate in a double-blind, randomized, cross-over in situ study.

Methods

Human enamel specimens with subsurface lesions were prepared and inserted into intra-oral appliances worn by volunteers. A slurry (1 g product plus 4 ml H₂O) of each product was rinsed for 60 s, 4 times per day for 10 days. Six products were tested (i) placebo, (ii) 1000 ppm F, (iii) 5000 ppm F, (iv) Tooth Mousse (TM), (v) TM plus 900 ppm F (TMP) and (vi) Clinpro with 950 ppm F. Calcium, inorganic phosphate and fluoride levels were measured in post-rinse/saliva samples using ion chromatography. Mineral content was measured using transverse microradiography.

Results

Only TM and TMP significantly increased salivary calcium and phosphate levels. The products produced remineralization in the following order from lowest to highest: placebo < 1000 ppm F = Clinpro < 5000 ppm F < TM < TMP.

Conclusion

Clinpro was not significantly different to 1000 ppm F whereas TM and TMP were superior to 5000 ppm F with TMP producing the highest level of enamel lesion remineralization.     

Analysis of the effect of CPP-ACP tooth mousse on enamel remineralization by circularly polarized images

Objective:To evaluate the effect of casein phosphopeptide–amorphous calcium phosphate tooth mousse on the remineralization of bovine incisor by circularly polarized images.Methods:Eighty bovine incisors, each with a 4 × 4 mm artificially demineralized area, were used. The samples were divided into four groups: Group A, casein phosphopeptide–amorphous calcium phosphate tooth mousse; Group B, fluoride toothpaste; Group C, casein phosphopeptide–amorphous calcium phosphate tooth mousse and fluoride toothpaste; and Group D, no treatment. Circularly polarized images were taken after the specimens were treated for 3, 6, 9, or 12 weeks, and the size of the demineralized area and the mean grey level were measured. Data analysis was done using repeated measures variance analysis. Pearson correlation coefficients were computed to evaluate the correlation between the size of the demineralized area and the mean grey level.Results:In all four groups, the size of the demineralized area and the mean grey level declined with time. The size of the demineralized area of Group C was significantly smaller than that of Group A at the end of the third and sixth weeks (P  =  .039, P  =  .000, respectively), and the mean grey level of Group C was lower than that of Group A at the end of the 6th and 12th weeks (P  =  .037, P  =  .004, respectively). At the end of the 6th, 9th, and 12th weeks, the size of the demineralized area of Group C was smaller (P  =  .000, P  =  .005, P  =  .005, respectively) and the mean grey level was lower (P  =  .000) than those of Group B. No statistically significant correlations were detected between the size of the demineralized area and the mean grey level.Conclusion:Casein phosphopeptide–amorphous calcium phosphate tooth mousse can reduce the size and mean grey level of demineralized areas and promote the remineralization of bovine enamel. Combined application with fluoride toothpaste strengthens the effect.