连苯三酚自氧化法测定超氧化物歧化酶活性的改进

改进后的连苯三酚自氧化法测定条件是:25℃,总体积4.5ml,50mM pH8.2Tris-HCl 缓冲液,连苯三酚的自氧化速率为0.070 O.D./min。本法具有操作简便,灵敏度高,用样量少等优点。     

超氧化物歧化酶两种测活方法的比较

超氧化物歧化酶（SOD）的测活方法很多，活性单位也不统一，该文研究了改进的肾上腺素法和微量连苯三酚法两种测活方法，比较了两者的自氧化曲线、重现性、灵敏度及测活结果。在严格控制实验条件的情况下，对两种方法的自氧化速率进行了相对标准偏差（RSD％）、线性回归（R）等统计分析。连苯三酚法在重现性（RSD4.72％，n=10）和灵敏度方面均高于肾上腺素法（RSD7.71％，n=10），连苯三酚法与肾上腺素法测活结果的比值约为2．21。     

邻苯三酚自氧化法测定血浆超氧化物歧化酶的方法学评价及临床应用探讨

目的对邻苯三酚自氧化法测定血浆超氧化物歧化酶(SOD)的方法学进行评价,并探索其临床应用价值。方法根据美国临床和实验室标准协会(CLSI)的评价方法,应用邻苯三酚自氧化法检测血浆SOD,评价其精密度、准确度、线性和回收率;随机选取2014年健康体检者42例作为对照组,2014年3-12月我院住院氧化应激反应相关疾病患者1580例作为疾病组,测定血浆SOD的活性水平,进行临床应用评价。结果用邻苯三酚自氧化法测定SOD精密度试验中批内精密度为1.57%和4.8%,批间精密度为2.17和2.21%;准确性试验相对偏倚为:1.6%和2.4%;线性试验在0~250 U/ml内R2为0.9978;回收率为96.2%~103.4%;SOD在各疾病组与健康对照组差异均具有显著性(P<0.01)。结论邻苯三酚自氧化法测定SOD方法学评价符合要求,其精密度高、准确性好,线性范围宽,具有较高的临床应用价值。     

邻苯三酚法测定超氧化物歧化酶缓释片中SOD的活性

目的建立超氧化物歧化酶 (SOD)缓释片的活性测定方法。方法采用邻苯三酚自氧化法。结果在SOD浓度为 0 .0 6 0～0 . 0 0 6mg·ml-1时线性关系良好 (r=0 .9996 ) ;平均空白加样回收率为 10 1 .94 % (n=9)。结论所用方法简单、快速 ,结果准确。     

超氧化物歧化酶的两种邻苯三酚测活法比较

为使SOD 测活结果规范化.对常规的两种邻苯三酚自氧化测活方法（420nm法和325nm法）进行了比较.结果发现.325nm法（邻苯三酚终浓度0.1mmol/L）测定的SOD活力为420nm法（邻苯三酚终浓度0.2 mmol/L）的 2.6倍左右,与以往报道不符,而且在低浓度下,邻苯三酚加量的变化会明显影响活性测定结果。     

邻苯三酚自氧化法测定SOD活性中测定波长的选择

用岛津UV—240型、260型和T—1901紫外可见分光光度计进行实验,确定邻苯三酚自氧化法测定SOD活性的最适波长非文献使用的420nm和325nm,而应为320nm。     

新疆葡萄干超氧化物歧化酶的分离提纯

本文首次对新疆葡萄干中超氧化物歧化酶进行了分离提纯.酶活力测定采用微量邻苯三酚自氧化法.结果表明:100g新疆葡萄干经粗抽提,丙酮两步沉淀,Sephadex G-100柱层析,DEAE-52柱层析,得2.73mg酶蛋白,比活为408.8u/mg pro,纯化倍数为92.9,达电泳纯.     

超氧化物歧化酶的药理作用

超氧化物岐化酶对辐射有防护作用,能减少糖尿病慢性并发症及肺部疾病,具抗炎活性,可减轻缺血再灌注损伤,预防心血管疾病,具抗肿瘤、抗衰老及免疫调节等多方面作用。此文对此进行了综述。     

钒酸盐氧化法和亚硝酸钠氧化法测定血清总胆红素的方法比较

目的:对钒酸盐氧化法和亚硝酸钠氧化法测定血清总胆红素进行方法比较。方法:采用两种方法测定血清总胆红素值,进行比较,同时对两种方法的重复性试验、线性试验、干扰试验以及回收试验进行分析总结。结果:两种方法有很好的相关性,钒酸盐氧化法和亚硝酸钠氧化法测定总胆红素(TB)批内变异系数分别为低值2.8%、2.0%,高值1.8%、1.3%;批间变异系数低值分别为2.8%、2.5%,高值分别为1.8%、1.4%;平均回收率分别为105%、102%;线性分别达到434.3μmol/L、500.0μmol/L;较轻微的溶血、脂浊、维生素C对两种方法测定TB无明显干扰。结论:亚硝酸钠氧化法与钒酸盐氧化法相比具有较好的重复性和回收率,但对溶血、脂浊、维生素C的抗干扰能力不及钒酸盐氧化法。     

修饰SOD活性测定方法的改进

修饰SOD已广泛用于化妆品和食品,但其活性测定方法目前国内尚无统一标准。由于酶表面修饰对酶活性测定有影响,本文报道了一种简便、快速的修饰SOD的测定方法。该法不仅准确而且实用。     

超氧化物歧化酶治疗复发性口腔溃疡的研究

目的　探讨超氧化物歧化酶在复发性口腔溃疡发病中的作用。 方法　 选择复发性口腔溃疡患者 5 4例 ,健康成人 5 1例 ,取静脉血用黄嘌呤氧化酶法测定血浆超氧化物歧化酶活性。 结果　复发性口腔溃疡患者的SOD较健康对照组明显低下 ,有显著性差异 (P <0 .0 1)。男女之间SOD值的差异无统计学意义 (P >0 .0 5 )。 结论　SOD的活性降低在ROU的发病机理中起重要作用     

Interaction Between Superoxide Dismutase and Dipalmitoylphosphotidylglycerol Bilayers: A Fourier Transform Infrared (FT-IR) Spectroscopic Study

Purpose. Superoxide dismutase (SOD), an antioxidant enzyme, converts peroxide radicals into hydrogen peroxide. Liposomes have been used as carriers for SOD to enhance its antioxidant effect. Our previous DSC study has suggested that SOD binding to dipalmitoylphosphatidylglycerol (DPPG) may protect lipid membranes against oxygen-mediated injury. We now present FT-IR studies on the effect of DPPG binding on the temperature-induced SOD folding-unfolding process. Methods. The FT-IR spectra of SOD in D₂O or DPPG membranes are measured as temperatures increase from 28° to 121°C at a rate of 0.5°C/ min. From the quantitative determination of the changes in the amide I band components of the Fourier self-deconvoluted spectra, the DPPG-induced changes of SOD secondary structure could be detected as a function of temperature. Results. We observe that the relative intensity of the SOD bands from 28°C to 77°C show graduate loss of -sheet distorted structure, loss of turns, and existence of an intermediate state around 50°C. Beginning at 80°C, changes are obtained in three temperature regions: (i) 80°C, (ii) 92°C, (iii) 109°C. The result suggests that SOD folding/unfolding transition involves mostly the relative changes within the regions of helix-like hydrogen bonding pattern, turn, twisted -bend and irregular structures. When SOD is bound to DPPG, the conformational changes shift to lower temperatures, indicating a reduction of SOD thermal stability. In addition, the gel to liquid crystalline phase transition temperature of DPPG increases from 42°C to 43.5°C. Conclusions. These results suggest that the thermal stability of SOD is reduced by DPPG binding. However, DPPG bilayer is stabilized by the presence of SOD.     

超氧化物歧化酶活性测定的间苯三酚替代试探

本文用间苯三酚替代邻苯三酚测定超氧化物歧化酶活性得到较为满意的效果.间苯三酚自氧化的适宜浓度为5mmol/L,pH8.8,348nm处有一特定紫外吸收峰.有较好的重复性.     

Activity of Superoxide Dismutase and Catalase in Fenugreek (Trigonella foenum-graecum) in Response to Carbendazim

Fenugreek (Trigonella foenum-graecum) is an annual herb, used as a spice and traditionally as medicine. Fenugreek finds its uses in treating hyperglycemia, hyperlipidemia and disorders of gastro-intestinal and cardiovascular systems. Fenugreek cultivation in India is affected by fungal diseases like root-rot and damping-off and fungicides like carbendazim are used to overcome these infections. Fungicides play both positive and negative role in plants; fungicides protect plants from diseases and also exert oxidative stress simultaneously. This report is on the response of antioxidants, superoxide dismutase and catalase in fenugreek seeds and plants treated to different concentrations of carbendazim.     

Folate-Conjugated Superoxide Dismutase Adsorbed Over Antioxidant Mimicking Nanomatrix Frameworks for Treatment of Rheumatoid Arthritis

Rheumatoid arthritis (RA) is an autoimmune disease occurring in larger population, characterized by synovial inflammation followed by destruction of joint. Major concerned factor for cause of RA has been related to oxidative stress due to environmental toxicity and immune imbalance. Reactive oxygen species generated from macrophages communes series of oxidation at cellular and genetic level and leads to generation of inflammatory cytokines for provoking inflammation in RA. Superoxide dismutase and catalase are supposed to be potential antioxidant enzymes to scavange free radicals. However, many clinical studies report reduced potency of enzyme due to lack of its targeting efficacy. Therefore, the aim of the work involves development of nanomatrix mimicking as catalase over which folate-linked superoxide dismutase was adsorbed for a macrophage targeting. The developed formulation was optimized, characterized for morphological studies, enzyme loading efficiency, protein activity, and in vitro drug release. Quantification of interleukin 6 was performed by indirect enzyme linked immunosborent assay over macrophage cell lines to determine in vitro treatment efficacy. It is concluded that the prepared system can act as enzyme reservoir to deliver acid labile enzymes in controlled form to efficiently treat RA.     

超氧化物歧化酶乳酸-羟乙酸共聚物微球的制备及其性质

利用复乳溶剂挥发法制备了超氧化物歧化酶（SOD）的乳酸－羟乙酸共聚物（PLGA）微球，考察了各工艺因素对微球粒径、包封率等的影响，通过扫描电子显微镜（SEM）、差示扫描量热分析（DSC）初步研究了其性质，结果表明，通过调整内水相的体积及浓度，分散相体积及PH值，可得到较高包封率，粒径在20－30μm，形态圆整，表面多孔的SOD微球，DSC表明SOD被有效地包入了PLGA微球中。     

乳糖诱导重组人SOD基因在大肠杆菌中的表达

利用乳糖代替IPTG诱导rhCu/Zn-SOD在大肠杆菌(Escherichia coli）BL21（DE3）中的表达,并将菌体生长情况及产物表达规律与IPTG诱导进行比较。对诱导所需的乳糖浓度、金属离子浓度等因素进行了研究,并分析了低温下诱导对重组蛋白表达的影响。最终在摇瓶发酵条件的基础上,实现了乳糖诱导重组菌的5L全自动发酵罐培养,rhCu/Zn-SOD酶活性达到1810U/ml(发酵液）。结果表明,乳糖可以诱导rhCu/Zn-SOD在Escherichia coli中的表达,并且酶活性达到IPTG诱导时的89%。     

小剂量维生素E、维生素C和肠溶阿司匹林对超氧化物歧化酶及其同功酶的影响

目的：对比观察肠溶阿司匹林、维生素E（VitE)、维生素C（VitC）3药合用与后两者合用，对超氧化物歧化酶（Sod）及其同功酶的影响。方法：将51例高血压患者、28例无合并症的冠心病患者，以及30例健康人群随机抽样各分为两组，观察服药前和服药后3年与5年的Sod及其同功酶的变化。结果：（1）总Sod活力及其同功酶的活力随高血压的病情加重而明显降低，与健康人群比较有显著性差异。（2）VitE和VitC合用可使Sod及其同功酶逐渐升高，与服药前比较有统计学差异（P＜0．05）。（3）3药合用升高更明显，与2药合用比较有极显著性差异（P＜0．01）；与服药前比较也有极显著性差异（P＜0．01）。（4）服药后3年与5年比较无显著性差异。结论：3药合用的抗衰老作用优于2药合用；口服小剂量肠溶阿司匹林、VitE和VitC的时间不应短于3年。