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. The study of single neuron response to photodynamic effect provides a means for the study of the dynamics of cytotoxic events
leading to cell death and allows comparison of the phototoxicity of different photosensitisers. Isolated crayfish stretch
receptor neurons were photosensitised for 30 min, then irradiated with a He-Ne laser (632.8 nm; 0.3 W/cm2) until irreversible firing cessation. The dynamics of neuron firing frequency were continuously recorded throughout. The
following photosensitisers were studied: methylene blue, janus green B, protoporphyrin IX, chlorins e
6 and p
6, haematoporphyrin derivative (Photoheme) and sulphonated aluminium phthalocyanine (Photosens). Nerve cells were found to
be insensitive to either He-Ne laser irradiation or photosensitisation alone, but very vulnerable to the photodynamic effect:
neurons changed firing rate and died at nanomolar concentrations of photosensitisers. The dynamics of neuron responses was
found to depend on photosensitiser type and concentration. The current approach provides a means of evaluation of initial
threshold cell membrane alteration and cytotoxic events leading to cell death. The dependence of firing acceleration rate
and neuron lifetime on photosensitiser concentration additionally allowed comparison of efficiencies of different photosensitisers.
Photosens, Photoheme and chlorin p
6 were found to be the most potent photosensitisers: neurons responded to their photodynamic effects at concentrations as low
as 1–5 nM.
Paper received 23 February 1998; accepted following revision 7 December 1998. 相似文献