苦玄参干浸膏抗炎镇痛作用的实验研究

目的:研究苦玄参干浸膏的抗炎及镇痛作用。方法:采用热板法、冰醋酸刺激致痛法测定小鼠痛阈;利用二甲苯制造小鼠耳廓炎症模型,测定小鼠耳片重量;采用腹腔染料渗出法,测定小鼠腹腔毛细血管通透性。结果:苦玄参提取物对小鼠热板及冰醋酸刺激致痛反应有明显镇痛作用,对二甲苯致小鼠耳廓肿胀、冰醋酸致腹腔毛细血管通透性增高均有明显的抑制作用。结论:苦玄参干浸膏有明显的抗炎及镇痛作用。     

木瓜中不同提取物的药效学研究

目的:比较研究木瓜总提物、木瓜苷、非木瓜苷的抗炎镇痛作用.方法:采用二甲苯引起的小鼠耳肿胀模型、醋酸致小鼠腹腔毛细血管通透性增加模型,观察木瓜中3种提取物的抗炎作用;采用热板法测定小鼠的痛阈,观察3种提取物的镇痛作用.结果:木瓜苷(60,120,240mg/kg)ig给药,对小鼠耳肿胀及醋酸致小鼠腹腔毛细血管通透性增加均有明显抑制作用,且抑制作用呈剂量相关趋势;木瓜苷(120,240mg/kg)ig给药,可以明显升高小鼠的痛阈,且镇痛作用呈剂量相关趋势.结论:木瓜苷具有抗炎、镇痛作用,确定木瓜苷为木瓜抗炎镇痛的有效部位.     

野木瓜不同提取部位的镇痛抗炎作用研究

目的探讨野木瓜不同部位提取物的镇痛抗炎效果,并作有效部位筛选。方法采用热板法和乙酸扭体法实验研究不同部位提取物的镇痛作用;采用二甲苯诱发小鼠耳廓肿胀模型研究不同部位提取物抗炎作用。结果野木瓜不同部位提取物对小鼠热板致痛均有明显的镇痛作用,其中乙醚提取物镇痛效果最为明显。不同部位提取物对乙酸诱发小鼠腹腔剧烈疼痛的镇痛作用显著,其中乙醚和三氯甲烷提取物效果尤为显著。乙醚提取物对二甲苯致小鼠耳廓肿胀有显著的抑制作用,而其他部位提取物抑制作用不明显。结论乙醚提取物为野木瓜中镇痛抗炎的主要活性部位。     

药理效应法对藏药熏倒牛不同提取工艺的评价

目的:研究藏药熏倒牛的提取工艺。方法:采用小鼠耳廓二甲苯致炎、小鼠热板法致痛实验,观察熏倒牛不同工艺提取物对抗炎、镇痛作用的影响。结果:熏倒牛传统水煎工艺提取物、传统水煎醇沉工艺提取物、醇提工艺提取物均有抑制二甲苯致小鼠耳肿胀的作用,均可提高小鼠热板法致痛的痛阈(P<0.01),但醇提取物的抗炎、镇痛效果明显优于水煎提取物和水煎醇沉提取物(P<0.01)。结论:熏倒牛乙醇提取物的抗炎、镇痛作用显著,其药理效应明显优于水煎和水煎醇沉工艺。     

开口箭抗炎镇痛作用实验研究

目的:探讨开口箭的抗炎镇痛作用。方法:抗炎实验用二甲苯致小鼠耳廓肿胀、角叉菜胶致大鼠足跖肿胀和大鼠棉球肉芽肿炎症模型,镇痛实验用热板法和醋酸扭体法。结果:开口箭水煎液灌胃100mL/(kg.d)能明显的抑制二甲苯致小鼠耳廓肿胀(P<0.05)。醇提液腹腔注射2mL/kg在1h、2h、3h时对角叉菜胶致大鼠足跖肿胀有非常明显的抑制作用(P<0.01)。水煎液腹腔注射2mL/kg对棉球埋藏引起的慢性肉芽肿有明显的抑制作用(P<0.01)。水煎液腹腔注射2mL/kg在60min时对热板致小鼠痛阈有明显提高(P<0.01)。水煎液皮下注射2mL/kg、0.5mL/kg对醋酸致小鼠扭体反应有明显的抑制作用(P<0.01)。结论:开口箭有明显的抗炎镇痛作用。     

虎威伤痛灵抗炎、消肿和镇痛作用的实验研究

目的:研究虎威伤痛灵的抗炎、消肿和镇痛的作用。方法:用耳廓肿胀法和蛋清致肿法观察虎威伤痛灵的抗炎作用;用病理组织学实验方法观察虎威伤痛灵对小鼠腿击伤肿胀的消肿作用;采用热板法和扭体法观察虎威伤痛灵的镇痛作用。结果:①虎威伤痛灵对二甲苯诱发小鼠耳廓肿胀和蛋清所致小鼠足跖肿胀有较好的抑制作用,对冰醋酸所致的小鼠腹腔毛细血管通透性增高有显著的降低作用;②对小鼠腿击伤部位作局部切片显微镜观察,结果显示肌纹较清晰、肌间脂肪组织增生,提示虎威伤痛灵对小鼠腿伤有一定的消肿作用;③对化学物质冰醋酸所致的扭体反应有较好的抑制作用,对热板引起小鼠痛阈无明显影响。结论:虎威伤通灵具有较好的抗炎、消肿和镇痛作用。     

栀子总苷的抗炎镇痛作用研究

目的研究栀子总苷(TGCJ)的抗炎镇痛作用。方法采用角叉菜致大鼠足肿胀模型、醋酸致小鼠腹腔毛细血管通透性增加模型、棉球致肉芽组织增生模型,观察TGCJ的抗炎作用;采用热板法测定小鼠的痛阈、醋酸诱发的小鼠扭体反应,观察TGCJ的镇痛作用。结果TGCJ的80、40mg/kg2个剂量组ig给药,对角叉菜致大鼠足肿胀、棉球致肉芽组织增生均有明显抑制作用,TGCJ的160、80、40mg/kg3个剂量组ig给药,对醋酸致小鼠腹腔毛细血管通透性增加有抑制作用,且抑制作用呈剂量相关趋势;TGCJ的160、80、40mg/kg3个剂量组ig给药,可以明显升高小鼠的痛阈,对醋酸诱发的小鼠扭体反应有一定抑制作用。结论TGCJ有明显的抗炎和一定的镇痛作用,确定TGCJ为栀子抗炎镇痛的有效部位。     

关龙胆乙醇提取物抗炎镇痛作用的研究

目的:观察关龙胆乙醇提取物的抗炎镇痛作用。方法:采用二甲苯、巴豆油致炎法观察关龙胆乙醇提取物对小鼠的抗炎作用;用冰醋酸扭体法和热板法观察关龙胆乙醇提取物对小鼠的镇痛作用。结果:关龙胆乙醇提取物能显著抑制二甲苯和巴豆油引起的小鼠耳廓肿胀,对热板及和冰醋酸所致小鼠疼痛及扭体反应。结论:关龙胆乙醇提取物具有一定的抗炎镇痛作用。     

竹节人参抗炎镇痛作用的实验研究

目的:探讨竹节人参的抗炎镇痛作用。方法:抗炎实验采用二甲苯致小鼠耳廓肿胀等常规抗炎模型;镇痛实验采用热板法和醋酸扭体法。结果:竹节人参提取物可明显抑制二甲苯致小鼠耳廓肿胀及角叉菜胶引起的大鼠足跖肿胀,能显著抑制大鼠棉球肉芽肿的增重及醋酸致小鼠扭体反应,可明显延长热板引起小鼠疼痛反应的痛阈值。结论:竹节人参有明显的抗炎镇痛作用。     

苦石莲提取物抗炎镇痛作用的实验研究

目的 观察苦石莲不同提取物的抗炎镇痛作用.方法 用二甲苯致小鼠耳肿胀法和角叉菜胶致大鼠足肿胀法研究抗炎作用,用热板法和扭体法研究镇痛作用.结果 苦石莲醇提取物8 g/kg、氯仿提取物4,8 g/kg对小鼠二甲苯致耳肿胀及角叉菜胶致大鼠足肿胀有抑制作用;苦石莲醇提取物4,8 g/kg、氯仿提取物4,8 g/kg能减少冰乙酸致小鼠扭体次数和提高热板致痛小鼠痛阈值.结论 苦石莲具有显著的抗炎和镇痛作用.     

十八症提取物的药理作用研究

目的 :研究十八症提取物的镇静、镇痛、抗抑郁及抗炎活性。方法 :以十八症的醋酸乙酯提取物和乙醇提取物按 50 ,100mg·kg^-1对小鼠分别灌胃给药 ,测定小鼠穿格行走数、扭体数、强迫游泳累计不动时间及耳廓肿胀率来考察十八症提取物的镇静、镇痛、抗抑郁、抗炎活性。结果 :醋酸乙酯提取物可使小鼠穿格行走数、扭体数、累计不动时间及耳廓肿胀率均明显减小 ,而乙醇提取物仅引起小鼠穿格行走数和累计不动时间显著减小。结论 :十八症的醋酸乙酯提取物有较显著的镇静、镇痛、抗抑郁及抗炎作用 ,而乙醇提取物仅在镇静、抗抑郁作用方面具一定活性。     

Evaluation of Antinociceptive Activity of Methanol Extract from Cleome rutidosperma in Mice

Objective Cleome rutidosperma (Capparidaceae), commonly known as “Fringed Spider Flower”, is a medicinal plant found in Southeast Asia. C. rutidosperma is used in folk medicine for diuretic, laxative, analgesic, anti-inflammatory, antipyretic, antimicrobial, anti-oxidant, hypoglycemic, and anthelmintic activities. We have evaluated the anti-nociceptive properties of methanol extract from C. rutidosperma (MECR) in vivo. Methods Thermal method (hot plate test and tail flick test) was induced to judge the anti-inflammatory effect and couple of chemical method also used (formalin induced licking test; writhing test carried by acetic acid) to evaluate analgesic effect. Both of these tests were made over animal models, like mice and rats. Two different doses (100 and 200 mg/kg) were used for each case of test, while morphine sulphate (5mg/kg, ip) was used as reference drug. Results MECR demonstrated the significantly anti-nociceptive activity in the analgesic and anti-inflammatory tests by reducing nociception in mice models (P < 0.001). In the hot-plate and tail-flick tests, MECR significantly elongated the time to response to the thermal stimuli (100 and 200 mg/kg with P < 0.05, 0.001). The remarkable increase in the latency was observed at 90 and 120 min. In acetic acid-induced writhing test and formalin induced licking test for anti-inflammatory activity, MECR at 100 and 200 mg/kg doses exhibited significant (P < 0.001) reduction of writhing and licking response. Conclusion The anti-inflammatory and analgesic effects of C. rutidosperma propose that this effect may be a result of both peripheral and central mechanisms. Further study is required to ensure the proper mechanism of action as well as the active ingredient.     

两种不同挥发油含量柴胡制剂药效作用研究

目的:对比两种不同挥发油含量柴胡制剂的抗炎、镇痛、解热作用。方法:分别采用二甲苯致炎剂所致小鼠耳廓肿胀实验,醋酸引起小鼠疼痛扭体实验,干酵母致大鼠发热实验,观察两种柴胡制剂的抗炎、镇痛、解热作用,并比较二者差异。结果:采用抗炎、镇痛、解热三种动物模型研究表明,普通柴胡浸膏5g生药/kg、10g生药/kg,富集挥发油的柴胡浸膏5g生药/kg、10g生药/kg均可显著减轻二甲苯所致的小鼠耳肿胀,减少醋酸所致的小鼠扭体反应次数,降低干酵母引起的大鼠体温升高,其中富集挥发油的柴胡浸膏的抗炎活性优于普通柴胡浸膏,但镇痛和解热活性两种浸膏无显著性差异。结论:两种不同挥发油含量柴胡浸膏均具有明显的抗炎、镇痛和解热作用,但柴胡挥发油抗炎活性较高。     

Anti-inflammatory and analgesic effects of the ethanol extract of Rosa multiflora Thunb. hips

This study aimed to assess the anti-inflammatory and analgesic effects of Fructus Rosae Multiflorae (FRM, hips of Rosa multiflora Thunb.). FRM was extracted with 75% ethanol and the dried extract (FRME) was administered intragastrically (i.g.) at 100, 200 and 400mg/kg. The anti-inflammatory effect was evaluated in four experimental animal models and analgesic effect in two animal models. Pretreatment with a single dose of FRME produced significant dose-dependent anti-inflammatory effects on carrageenin-induced rat hind paw edema, xylene-induced mouse ear edema and acetic acid-induced mouse vascular permeation. In a 7-day study, daily administration of FRME suppressed cotton pellet-induced rat granuloma formation. Pretreatment with a single dose of FRME also produced dose-dependent anti-nociceptive effects in thermally- and chemically induced mouse pain models. In addition, a single dose of FRME at 2.4g/kg body weight (equivalent to 87.6g of dried hips per kg body weight) produced no observable acute toxicity in mice within seven days. These results demonstrate that FRME possesses anti-inflammatory and analgesic effects and has no obvious acute toxicity, which advanced our understanding of the folk use of FRM in treating various inflammatory disorders.     

Anti-nociceptive and anti-inflammatory activity caused by Cistanche deserticola in rodents

In the present study, the rhizomes of Cistanche deserticola (Orobanchaceae, abbreviated as CD) were extracted with 50% ethanol and isolated orderly by ethyl acetate, n-butanol and water. The analgesic and anti-inflammatory effects of CD extract and three layers were evaluated in several animal models. CD extracts effectively inhibited writhing response induced by 1% acetic acid and biphasic licking responses caused by 1% formalin, and also reduced the edema induced by 1% carrageenan but not zymosan. Furthermore, the butanolic and aqueous layers of CD extract not only reduced the pain induced by acetic acid and formalin, but also decreased the edema that induced by carrageenan. Effects of the butanolic layer of CD extract are better than that of the aqueous layer. In addition, the effect of the butanolic layer of CD extract was not abolished by naloxone. These results revealed that CD has analgesic and anti-inflammatory effects, and the butanolic and aqueous layers are mainly active constituents. Furthermore, the analgesic and anti-inflammatory effects of the butanolic layer of CD extract were not related to opioid receptors and immune system.     

Anti-nociceptive and anti-inflammatory activity of sophocarpine

Aim of the study

This study aimed to assess the anti-nociceptive and anti-inflammatory activity of sophocarpine in rodents.

Materials and methods

The anti-nociceptive effect was evaluated in two experimental animal models and anti-inflammatory effect in three animal models, and sophocarpine was given by tail vein injection for the above study.

Results

Pretreatment with a single dose of sophocarpine (20 and 40 mg/kg) produced dose-dependent anti-nociceptive effects in thermally and chemically induced mouse pain models. Pretreatment with a single dose of sophocarpine also produced significant dose-dependent anti-inflammatory effects on carrageenan-induced rat hind paw edema (15 and 30 mg/kg), xylene-induced mouse ear edema (20 and 40 mg/kg) and acetic acid-induced mouse vascular permeation (20 and 40 mg/kg).

Conclusions

Sophocarpine possesses peripheral and central analgesic properties as well as anti-inflammatory activity on acute inflammatory processes.     

Anti-inflammatory, analgesic activity and acute toxicity of Sida cordifolia L. (Malva-branca)

Sida cordifolia L. (Malvaceae) is used in folk medicine for the treatment of inflammation of the oral mucosa, blenorrhea, asthmatic bronchitis and nasal congestion. The anti-inflammatory, analgesic effects and acute toxicity of an aqueous extract of S. cordifolia were evaluated in animal models. The extract was prepared using leaves collected before the flowering period. The aqueous extract (AE) showed a significant inhibition of carrageenin-induced rat paw edema at a dose of 400 mg/kg administered orally, but did not block the edema induced by arachidonic acid. The AE also increased the latency period for mice in the hot plate test, and inhibited the number of writhes produced by acetic acid at the oral dose of 400 mg/kg. The aqueous extract of S. cordifolia showed low acute toxicity in mice.     

葛根麻黄颗粒药效学研究

目的:考察葛根麻黄颗粒的抗炎、镇痛、解热、免疫作用.方法:用巴豆油致小鼠耳廓肿胀和小鼠腹腔毛细血管通透性实验观察其抗炎作用;用小鼠热板法和小鼠扭体法评价其镇痛作用;用副伤寒三联菌苗致家兔发热和酵母致大鼠发热实验观察其解热作用;用炭粒廓清试验和羊红细胞(SRBC)致小鼠迟发性超敏反应(DTH)试验评价其对免疫功能的影响.结果:葛根麻黄颗粒生药18、36 g/kg能明显抑制巴豆油引起的小鼠耳廓炎症肿胀,降低醋酸引起的小鼠腹腔毛细血管通透性增高;葛根麻黄颗粒生药9、18、36 g/kg能够明显减少醋酸引起的小鼠扭体次数,提高小鼠热板痛阀值;葛根麻黄颗粒生药9、18、36 g/kg对酵母所致大鼠发热有明显的解热作用,生药36 g/kg对副伤寒三联菌所致家兔发热有明显的解热作用;葛根麻黄颗粒生药18、36 g/kg还可明显增强小鼠的吞噬细胞的功能及吞噬速率,生药36 g/kg明显抑制SRBC致小鼠迟发过敏反应.结论:葛根麻黄颗粒具有明显的抗炎、镇痛、解热、免疫增强作用.     

牛白藤抗炎镇痛作用有效部位的筛选

目的比较研究牛白藤的不同溶剂提取物的抗炎镇痛作用,为牛白藤的临床应用以及筛选分离活性成分提供实验依据。方法采用二甲苯致小鼠耳廓肿胀和醋酸致小鼠毛细血管通透模型评价药物的抗炎作用,采用小鼠热刺激致痛和醋酸扭体模型评价镇痛作用,比较牛白藤水提物、醇提物及不同溶剂萃取物的抗炎镇痛作用。结果牛白藤水提物、醇提物均具有抗炎镇痛作用,同剂量之间比较以醇提物活性较强;牛白藤石油醚、乙酸乙酯萃取物能明显减轻二甲苯诱导的小鼠耳肿胀程度,抑制热刺激和醋酸引起的小鼠疼痛反应。结论牛白藤具有明显的抗炎镇痛活性,石油醚萃取物和乙酸乙酯萃取物可能是其主要有效部位。