大叶小檗根不同萃取物的抗炎活性研究

目的 研究大叶小檗根石油醚、乙酸乙酯萃取物对小鼠耳廓炎症的抑制作用.方法 采用二甲苯、巴豆油致小鼠耳廓炎症,观察并计算小鼠耳肿胀度,确定大叶小檗根的抗炎活性.结果 大叶小檗根的乙酸乙酯萃取物能明显抑制二甲苯和巴豆油引起的小鼠耳廓炎性肿胀;石油醚萃取物对巴豆油引起的小鼠耳廓炎性肿胀有明显的抑制作用.结论 大叶小檗根乙酸乙酯萃取物的抗炎活性强于石油醚萃取物,大叶小檗根具有一定的抗炎活性.     

洋甘菊挥发油抗炎作用的研究

采用动物体内抗炎实验模型等方法研究洋甘菊挥发油的抗炎效果。观察洋甘菊挥发油的体内抗炎作用。结果表明:洋甘菊挥发油各剂量组对蛋清致大鼠足肿胀、棉球植入法致大鼠肉芽增生及二甲苯致小鼠耳肿胀均有不同程度的抑制作用。洋甘菊挥发油对急性炎症、慢性炎症及对急性炎症引起的肿胀、渗出有一定的抑制作用,洋甘菊挥发油有显著的抗炎作用。     

大叶秦艽花与麻花秦艽花抗炎镇痛作用的研究

目的研究大叶秦艽花与麻花秦艽花的抗炎镇痛作用。方法采用二甲苯所致小鼠耳肿胀和大鼠角叉菜胶足趾肿胀模型,研究大叶秦艽花与麻花秦艽花的抗炎作用;采用热板法、醋酸扭体法、光照甩尾法,研究大叶秦艽与麻花秦艽的镇痛作用。结果大叶秦艽、麻花秦艽能减轻二甲苯所致小鼠耳肿胀的体积和大鼠角叉菜胶足趾肿胀率;提高热板法引起小鼠的痛阈值及延长光电甩尾法所致的潜伏期;显著地减少醋酸引起的扭体次数。结论大叶秦艽花与麻花秦艽花具有较好的抗炎、镇痛作用。     

中药秦艽抗炎镇痛作用的实验研究

目的 研究中药秦艽的抗炎镇痛作用.方法 用醋酸扭体法、热板法观察秦艽水煎液对小鼠的镇痛作用;用二甲苯致小鼠耳廓肿胀、角叉菜胶致足跖肿胀的方法观察秦艽水煎液的抗炎作用.结果 秦艽水煎液30g/kg能减少小鼠扭体次数,提高小鼠痛阈值(P＜0.01),明显抑制小鼠耳廓肿胀和足跖肿胀(P＜0.01).结论 秦艽具有一定的抗炎、镇痛作用.     

秦艽提取物对一氧化氮合酶、磷脂酶A2和环氧化酶的影响

目的:探讨龙胆苦苷及秦艽不同极性提取部位的抗炎机制.方法:采用脂多糖(LPS)诱导鼠巨噬细胞RAW264.7生成一氧化氮(NO)、磷脂酶A2(PLA2)、环氧化酶(COX-2),建立细胞炎症模型.观察龙胆苦苷及大叶秦艽不同极性提取部位对NO、PLA2、COX-2的影响.结果:龙胆苦苷及秦艽不同极性提取部位对NO、PLA2均有抑制作用,仅龙胆苦苷对COX-2呈现出抑制作用.结论:龙胆苦苷是秦艽中主要的抗炎成分其抗炎机制与抑制NO、PLA2、COX-2的活性有关,秦艽中除龙胆苦苷以外的化学成分也具有抗炎作用,可能是由于抑制NO、PLA2而介导的.     

七叶莲不同溶剂提取部分的抗炎镇痛作用

目的探讨七叶莲不同溶剂提取部分的抗炎镇痛作用。方法采用醋酸扭体法、小鼠耳二甲苯致炎法、肿胀法等方法评价七叶莲不同提取部分的抗炎镇痛效果,并明确其抗炎镇痛有效部分。结果七叶莲叶挥发油可明显减少小鼠醋酸所致的扭体次数,可明显缓解小鼠二甲苯所致的耳廓肿胀;七叶莲茎水提液氯仿部分、正丁醇部分也可减少小鼠醋酸所致扭体次数,有效缓解小鼠二甲苯所致的耳廓肿胀和角叉菜胶所致的足趾肿胀。结论七叶莲抗炎镇痛药理作用的有效部分主要集中在叶挥发油部分、茎水提液的氯仿部分和正丁醇部分。     

东北贯众抗炎镇痛作用有效部位研究

目的:确定东北贯众抗炎镇痛作用的有效部位,为进一步研究东北贯众抗炎镇痛作用的活性成分奠定基础.方法:热板法测定痛阈值,观察其镇痛作用;二甲苯诱发小鼠耳廓肿胀及甲醛致足跖肿胀观察其抗炎作用,研究东北贯众抗炎镇痛的有效部位.结果:东北贯众对小鼠热板致痛、二甲苯所致的小鼠耳肿胀及甲醛致小鼠足跖肿胀均有一定的抑制作用,且30%...     

银芩口服液的抗炎解热作用及对小鼠免疫功能的影响

[摘要]目的：研究银芩口服液抗炎、解热及对小鼠免疫系统的影响。方法：建立二甲苯致小鼠耳廓肿胀、鸡蛋清致小鼠足趾肿胀抗炎,大肠埃希菌内毒素致家兔发热和环磷酰胺致小鼠免疫低下模型。观察银芩口服液的抗炎、解热作用及对免疫低下小鼠巨噬细胞吞噬活性及血清溶血素的影响。结果：银芩口服液在2.5 g/kg、5.0 g/kg剂量下能显著减轻二甲苯所致小鼠耳廓肿胀和鸡蛋清致小鼠足趾肿胀,并降低发热家兔体温（持续12 h）。同时银芩口服液还能提高小鼠巨噬细胞吞噬活性,促进溶血素生成（P<0.01）。结论：银芩口服液具有良好的抗急性炎症、解热作用,且能提高小鼠免疫功能。     

黄槿抗炎活性部位的初探

目的研究黄槿抗炎活性部位。方法采用70%乙醇提取药材,提取物用乙酸乙酯、正丁醇萃取,利用二甲苯致小鼠耳肿胀和醋酸致小鼠毛细血管通透性模型,观察黄槿提取物及其萃取物的抗炎活性。结果黄槿70%乙醇提取物及乙酸乙酯萃取物对二甲苯所致小鼠耳肿胀、醋酸致小鼠毛细血管通透性增加均具有显著抑制作用,与空白组比有显著差异(P<0.05),而正丁醇萃取物与生理盐水组对比没有显著差异(P>0.05)。结论黄槿提取物具有显著抗炎作用,并初步确定有效部位为乙酸乙酯萃取物。     

鄂产竹节参多糖的抗炎、镇痛活性

目的:提取分离竹节参多糖,初步研究其化学组成及评价抗炎镇痛的活性。方法:采用化学法测定竹节参多糖的成分,红外光谱分析其结构;采用二甲苯致耳肿胀模型、腹腔毛细血管通透性试验和小鼠热板、扭体试验分别考察其抗炎、镇痛效果。结果:分析显示竹节参多糖中含有α-吡喃糖环结构;竹节参多糖能够有效延长小鼠痛阈值,减少扭体次数,抑制耳肿胀,降低毛细血管通透性。结论:竹节参多糖为竹节参中抗炎镇痛活性成分之一。     

Anti-inflammatory effects of 4-o-methyl-benzenesulfonyl benzoxazolone (MBB) in vivo and in vitro as a novel NSAIDs lead compound

Background

Great attention has been paid to the development of novel anti-inflammatory drugs to overcome the adverse reactions of traditional drugs. Recently, a new compound 4-o-methyl-benzenesulfonyl benzoxazolone (MBB) we have prepared attracted our attention for its promising anti-inflammatory activity and low toxicity. The present study aimed to further investigate the anti-inflammatory effects of MBB both in vivo and in vitro in order to determine its potential as a novel NSAIDs lead compound.

Anti-inflammatory and anti-nociceptive activities of compounds from <Emphasis Type="Italic">Tinospora sagittata</Emphasis> (Oliv.) Gagnep.

Radix Tinosporae is a herb widely used in traditional Chinese medicine for the treatment of various inflammatory diseases. In the present study, its anti-inflammatory and antinociceptive activities were investigated. The ethanol extract of Radix Tinosporae exhibited significant inhibitory effects on xylene-induced ear edema and acetic acid-induced writhing in mice. Using bioassay-guided fractionation, the n-butanol fraction was determined as the active fraction. Further purification of the most active n-butanol fraction led to the isolation of three compounds, palmatine, columbamine and columbinyl glucoside. All three compounds showed inhibitory activities on xylene-induced ear edema, but only palmatine and columbamine exhibited significant inhibitory effects on acetic acid-induced writhing. In addition, palmatine and columbamine markedly inhibited in vitro production of nitric oxide and nuclear factor-κB activation in RAW264.7 macrophage cells in response to lipopolysaccharide or tumor necrosis factor α stimulation. These results provide justification for the utilization of Radix Tinosporae in Chinese folk medicine for the treatment of inflammatory diseases.     

Evaluation of Parmotrema reticulatum Taylor for Antibacterial and Antiinflammatory Activities

Lichens produce variety of secondary metabolites including depsides, depsidones and dibenzofurans having multifunctional activity in response to external environmental condition. Present study provides evidence for in vitro antibacterial and in vivo antiinflammatory activity of acetone and ethanol extracts of Parmotrema reticulatum. In vitro antibacterial activity was investigated against gram positive and gram negative bacteria. Cotton pellet-induced granuloma, xylene-induced ear swelling, carragennan-induced edema, histamine-induced and carboxymethylcellulose sodium-induced leukocyte emigration in mice models were used to quantify the antiinflammatory activity. Acetone and ethanol extracts were showed antibacterial activity against Bacillus subtilis (minimal inhibitory concentration: 100 and 150 μg/ml) and Staphylococcus aureus (minimal inhibitory concentration: 100 and 200 μg/ml), Escherichia coli (minimal inhibitory concentration: 200 and 250 μg/ml), and Pseudomonasa eruginosa (minimal inhibitory concentration: 200 and 300 μg/ml). Acetone extract was inhibited edema significantly at 200 mg/kg with xylene, cotton pellet, carragennan and histamine induced edema in vivo models. Ethanol extract was found effective at dose of 300 mg/kg with all in vivo antiinflammatory models. The results showed significant (P<0.01) antiinflammatory effects at 200 and 300 mg/kg dose of acetone and ethanol extracts, respectively, which can be concluded that significant activity may be due to presence of flavanoids in ethanol extract and usnic acid in acetone extract.     

Study on chemical composition,anti-inflammatory and anti-microbial activities of extracts from Chinese pear fruit (Pyrus bretschneideri Rehd.)

Pyrus bretschneideri Rehd. belongs to the genus Pyrus, a member of Rosaceae family. It is a routine edible fruit, and also used as a folk medicine to treat cough, eliminate constipation, and relieve alcoholism. In order to clarify the active compounds of P. bretschneideri, the phytochemical study were performed. Five compounds were isolated and identified as 2β,19α-dihydroxy ursolic acid, quercitrin, dibutyl phthalate, diisobutyl phthalate and α-amyrin. Additionally, we investigate the anti-inflammatory and anti-microbial effects of the fractions of P. bretschneideri partitioned with petroleum ether, ethyl acetate and n-butanol, respectively. In the carrageenan-induced rat paw edema test, the EtOAc fraction showed the strongest inhibition of edema formation 0.5–5 h after edema induction, followed by n-butanol. EtOAc also displayed potent anti-inflammatory activity against xylene-induced ear edema (22.03% and 43.69%, respectively) and acetic acid-induced extravasation of Evan’s blue dye (39.58% and 49.92%, respectively) at the dose of 200 and 400 mg/kg. While, the anti-microbial results showed that ethyl acetate and n-butanol fractions exhibited strong activity against the bacteria strains. Moreover, 2β,19α-dihydroxy ursolic acid, α-amyrin and quercitrin could significantly inhibit the ear edema induced by xylene at the dose of 20 mg/kg, and exhibited moderate anti-microbial activities against the bacteria strains.     

月矾栓抗炎镇痛作用研究

目的研究月矾栓的抗炎镇痛作用。方法采用二甲苯致小鼠耳廓肿胀、角叉菜胶致小鼠足跖肿胀模型,观察月矾栓的抗炎作用;采用小鼠醋酸扭体法和热板法,观察月矾栓的镇痛作用。结果月矾栓对醋酸引起的小鼠扭体反应有明显的抑制作用,能明显提高小鼠热板痛阈值;月矾栓能抑制二甲苯引起的小鼠耳廓肿胀和角叉菜胶引起的小鼠足跖肿胀度,其中对小鼠耳肿胀和醋酸扭体的抑制作用呈明显量效关系。结论月矾栓有明显的抗炎、镇痛作用。     

番红花不同药用部位的镇痛和抗炎活性比较研究

目的:扩大名贵药用植物番红花的药用资源,寻找可以替代传统药用部位(柱头)的新药用部位.方法:分别采用醋酸致小鼠扭体和二甲苯致小鼠耳肿胀药理模型,分别以吲哚美辛和地塞米松为镇痛和抗炎药效学实验的阳性对照药,观察番红花球茎、顶芽、侧芽和柱头的乙醇和水提取物的镇痛和抗炎活性.结果:在镇痛实验中,与空白对照组相比,顶芽醇提取物和水提取物、柱头的醇提物和水提物以及侧芽的醇提物在高剂量时(相当于2.0 g生N/kg)都具有显著的镇痛活性(P＜0.05或P＜0.01);球茎的醇提物和水提物、侧芽醇提取物的低剂量和中剂量以及水提物均未见有镇痛活性;在抗炎试验中,与空白对照组相比,番红花柱头醇提物和侧芽醇提物均显示出显著的抗炎活性(P＜0.05或P＜0.01).项芽醇提物在低剂量(相当于0.75 g生药/kg)时也具有显著的抗炎活性(P＜0.05).球茎醇提取物和水提物及顶芽、侧芽和柱头的水提物对二甲苯致小鼠耳肿胀没有显著性影响.结论:以番红花的顶芽、侧芽替代传统药用部位柱头,分别用于镇痛和抗炎作用有潜在的应用价值,值得深入研究.     

臭牡丹提取物抗炎镇痛抗过敏作用的实验研究

目的：探讨臭牡丹提取物的抗炎、镇痛、抗过敏作用。方法：以醋酸致小鼠腹腔毛细血管通透法、二甲苯致小鼠耳肿胀法观察其抗炎作用；以小鼠醋酸扭体法观察其镇痛作用；以2，4-二硝基氟苯（DNFB）致敏法观察其抗过敏作用。结果：臭牡丹提取物显著抑制小鼠腹腔毛细血管炎性渗出，抑制二甲苯所致小鼠耳廓肿胀。减少醋酸致小鼠扭体次数；并降低DNFB诱导的小鼠过敏反应。结论：臭牡丹提取物具有较好的抗炎、镇痛及抗过敏作用，与其相关的中医临床疗效相符。     

苯磺酰基呋咱氮氧化物与双氯芬酸偶联化合物的合成及抗炎活性

目的　研究高效低毒的双氯芬酸(DC)偶联化合物。方法　以酯键或酰胺键将一氧化氮(NO)供体3 ,4-二苯磺酰基呋咱氮氧化物与DC偶联,观察偶联物对二甲苯致炎小鼠和角叉菜胶致炎大鼠的抗炎活性及对大鼠胃肠道反应,研究体内外偶联物的NO释放。结果　合成了11个新化合物(I_1-11) ,其结构经MS ,IR ,¹HNMR和元素分析确证。I_1-5,I₉显示抗炎活性,其中I₄ 和I₅活性与DC相当,胃肠道副作用显著小于DC ,体内外均释放NO。结论　苯磺酰基呋咱氮氧化物与DC偶联的化合物可保留DC抗炎活性,降低DC胃肠道不良反应。     

α-取代的对甲磺酰基苯丙烯酰胺的合成及抗炎活性

目的寻找高效低毒的非甾体抗炎药。方法合成α-取代的对甲磺酰基苯丙烯酰胺，评价其抗炎活性，并考察连续经口给药对大鼠胃肠道（GI）的影响。结果合成了25个新化合物（II1-25），其结构经IR、¹H NMR、MS和元素分析确证。角叉菜胶致大鼠足跖肿胀模型试验结果显示，12个化合物(II1,3,5,7,8,10-12,17,18,20,23)的抗炎活性与双氯芬酸钠（DC）和罗非昔布（RC）相当（P>0.05）。其中II3,8,10,11,18,20的GI副作用均显著小于DC(P<0.01)，与RC和羧甲基纤继素钠(CMC-Na)无明显差别(P>0.05)。结论α-取代的对甲磺酰基苯丙烯酰胺抗炎活性强，GI不良反应低，值得深入研究。     

麝香祛痛气雾剂的抗炎镇痛作用与毒理学考察

目的:研究麝香祛痛气雾剂局部给药的抗炎镇痛作用及毒性反应。方法:用二甲苯致小鼠耳肿胀和角叉菜胶致大鼠足跖肿胀,考察麝香祛痛气雾剂的抗炎作用;用小鼠醋酸扭体法和热板法,观察麝香祛痛气雾剂的镇痛作用。观察家兔一次性局部给药的急性毒性和皮肤刺激性,观察豚鼠给药的皮肤致敏性。结果:麝香祛痛气雾剂局部皮肤给药,可明显减轻小鼠耳肿胀度,降低角叉菜胶致大鼠足跖肿胀;对小鼠醋酸扭体和热板反应均呈剂量相关性镇痛作用;对家兔和豚鼠无明显急性毒性反应、刺激性和致敏作用。结论:麝香祛痛气雾剂局部给药,具有明显的抗炎镇痛作用,无明显毒性、刺激性和致敏性。