维吾尔药大戟脂中2个新的三萜

目的 研究大戟脂(多脂大戟Euphorbia resinifera的树脂状分泌物)的化学成分.方法 采用反相C18、硅胶、凝胶柱色谱、制备型HPLC等方法进行分离纯化,通过红外、紫外、高分辨质谱、一维和二维的核磁数据和结合参考文献鉴定化合物的结构.结果 从大戟脂甲醇提取物中分离得到了2个化合物,分别鉴定为大戟烷-8-烯...     

Four new triterpenoids from Maytenus ilicifolia

Four new triterpenoids with various skeletons, maytefolins A-C (1-3) and uvaol-3-caffeate (4), were isolated from the leaves of a Brazilian medicinal plant, Maytenus ilicifolia, together with five known triterpenoids. Of these triterpenoids only erythrodiol exhibited significant cytotoxicity against KB/S, KB/VJ300, and KU 19-20 cells.     

Three new triterpenoids from Peganum nigellastrum

Three new triterpenoids, 3alpha,27-dihydroxylup-20(29)-en-28-oic acid methyl ester, 3alpha-acetoxy-27-hydroxylup-20(29)-en-28-oic acid methyl ester, and 3alpha-acetoxyolean-12-ene-27,28-dioic acid 28-methyl ester, were isolated from the roots of Peganum nigellastrum along with four known lupene-type triterpenoids. The structures of the new triterpenoids were determined by NMR spectroscopic means. The new triterpene, 3alpha, 27-dihydroxylup-20(29)-en-28-oic acid methyl ester is a DNA topoisomerase II inhibitor (IC(50) = 8.9 microM/mL).     

商陆营养器官中蛋白质量与生长龄动态变化关系研究

目的为商陆的开发和利用提供依据。方法利用光学显微技术、组织化学定位方法对美洲商陆Phytolacca decandra不同生长龄的根、茎和叶进行解剖结构研究。结果除幼根外，不同生长龄的营养器官中均有活性蛋白质分布，薄壁细胞是活性蛋白质分布的场所。结论幼茎中蛋白质的量最为丰富，颗粒也较大，与老叶、幼叶、老茎和老根中蛋白质的数量及大小差异极显著；二年生的贮藏根中不含蛋白质，额外形成层中几乎不含蛋白质。     

反柄紫芝中2个新三萜类化合物

目的研究反柄紫芝Ganoderma cochlear子实体的化学成分。方法利用硅胶和凝胶柱色谱及半制备HPLC进行分离纯化,采用多种谱学技术(1D、2D NMR)并根据理化常数鉴定结构。结果分离并鉴定了5个化合物,分别为拱状灵芝素G(1)、拱状灵芝素H(2)、麦角甾醇(3)、5α,8α-环二氧-6,22-麦角甾二烯-3β-醇(4)和(22E,24R)-7,22-麦角甾二烯-3β-醇(5)。结论化合物1和2为新化合物,其余化合物均为首次从灵芝属真菌中分离得到。     

商陆营养器官中蛋白质量与生长龄动态变化关系研究

目的为商陆的开发和利用提供依据。方法利用光学显微技术、组织化学定位方法对美洲商陆Phytolacca decandra不同生长龄的根、茎和叶进行解剖结构研究。结果除幼根外,不同生长龄的营养器官中均有活性蛋白质分布,薄壁细胞是活性蛋白质分布的场所。结论幼茎中蛋白质的量最为丰富,颗粒也较大,与老叶、幼叶、老茎和老根中蛋白质的数量及大小差异极显著;二年生的贮藏根中不含蛋白质,额外形成层中几乎不含蛋白质。     

A new prenylated isoflavone and triterpenoids from Erythrina eriotriocha

In addition to known triterpenoids maniladiol [6] and serrat-14-ene-3 beta,21 alpha-diol [7], two new compounds, one oleanane derivative designated as 28-acetoxyerythrodiol [1] and one isoflavone designated as 5,4'-dimethoxy-3'-prenylbiochanin A [5], have been isolated and characterized from the CHCl3 extract of the stem bark of Erythrina eriotriocha. Their structures have been established on the basis of the spectrocopic techniques (ir, nmr, uv, ms) and chemical evidence.     

Tyromycic acids B-E, new lanostane triterpenoids from the mushroom Tyromyces fissilis

Four new triterpenoids, tyromycic acids B-E (1-4), were isolated from a methanol-soluble extract of the fruit bodies of the Japanese inedible mushroom Tyromyces fissilis. Tyromycic acids B-D possess a lanostane skeleton, while tyromycic acid E is based on a rare 14(13-->12)abeo-lanostane skeleton. Their structures were determined by spectral data analysis and by single-crystal X-ray crystallography.     

Two new triterpenoids from Azadirachta indica and their insecticidal activity

Two new triterpenoids, 22,23-dihydronimocinol (1) and desfurano-6alpha-hydroxyazadiradione (2), were isolated from a methanolic extract of the fresh leaves of Azadirachta indica (neem) along with a known meliacin, 7alpha-senecioyl-(7-deacetyl)-23-O-methylnimocinolide. The structures of 1 and 2 were elucidated through spectral and chemical studies. Compounds 1 and 2 showed mortality for fourth instar larvae of the mosquito (Anopheles stephensi), with LC(50) values of 60 and 43 ppm, respectively.     

Cassipoureamide-A and -B: new sulfur-containing amides from stem wood of Cassipourea guianensis

New sulfur-containing amides, cassipoureamide-A (1) and -B (2), have been isolated from the stem wood of Cassipourea guianensis, and their structures were determined to be cis-N-(2-hydroxyethyl)-4-hydroxy-1,2-dithiolane-3-carboxamide and cis-N-(2-hydroxyethyl)-5-hydroxy-1,2,3-trithiane-4-carboxamide, respectively, by MS and NMR studies.     

江南山梗菜中1个新的三萜类化合物及其生物活性研究

目的 对江南山梗菜Lobelia davidii中的三萜类成分及其生物活性进行研究。方法 采用硅胶柱色谱、Sephadex LH-20及HPLC等色谱技术进行分离纯化,运用NMR、MS等波谱技术进行化合物的结构鉴定。采用四甲基偶氮唑盐[3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide,MTT]法筛选分离得到化合物对人非小细胞肺癌A549细胞的细胞毒性;采用4-硝基苯基-α-D-吡喃葡萄糖苷(4-nitrophenyl-α-D-glucopyranoside,PNPG)法测定分离得到化合物对α-葡萄糖苷酶的抑制活性。结果 从江南山梗菜的醋酸乙酯部位中分离得到6个三萜类化合物,分别鉴定为3β-正十六酸酯-齐墩果烷-12-烯-11-酮(1)、熊果酸(2)、齐墩果酸(3)、sumaresinolic acid(4)、3β-羟基-齐墩果烷-12-烯-11-酮(5)、32,33,34-三甲基-细菌藿烷-16-烯-3-O-β-D-吡喃葡萄糖苷(6)。细胞毒活性筛选结果表明,化合物1和5对A549细胞增殖有显著的抑制活性,其半数...     

Kadcoccilactones A-J, triterpenoids from Kadsura coccinea

Ten new highly oxygenated triterpenoids, kadcoccilactones A-J (1-10), and two known triterpenoids, kadsuphilactone A and micrandilactone B, were isolated from the stems of the evergreen climbing plant Kadsura coccinea. The structures of the new compounds were elucidated by spectroscopic evidence, with that of 1 confirmed by single-crystal X-ray diffraction analysis. This is the first report on nortriterpenoids (2, 4-6, 8-10) from the genus Kadsura.     

Agladupols A-E, triterpenoids from Aglaia duperreana

Three new apotirucallane-type triterpenoids, agladupols A-C (1- 3), and two new tirucallane-type triterpenoids, agladupols D and E (4 and 5), along with four known compounds, were isolated from the leaves and stems of Aglaia duperreana (Meliaceae). The structures of compounds 1- 5 were elucidated by spectroscopic data. A (13)C NMR-based general rule for assignment of the C-21 configuration in the side chain of apotirucallane- and tirucallane-type triterpenoids was proposed. According to this, the relative stereochemistry of 21- O-methyltoosendanpentol (1a), a known compound with the relative configurations of the stereocenters in the side chain undetermined, was completely assigned.     

Four new trisnorlanostene-type triterpenoids from the stem bark of Pinus luchuensis

Four new 25,26,27-trisnorlanostene-type triterpenoids were isolated from the stem bark of Pinus luchuensis, together with five known compounds. These new compounds were characterized as 25,26, 27-trisnor-3 alpha-hydroxy-lanost-9(11)-en-24-oic acid (1), 25,26, 27-trisnor-3 alpha-methoxy-lanost-9(11)-en-24-oic acid (2), 25,26, 27-trisnor-3 beta-methoxy-lanost-9(11)-en-24-oic acid (3), and 25,26, 27-trisnor-3-oxo-lanost-9(11)-en-24-oic acid (4) on the basis of MS and NMR evidence.     

Isolation and characterization of two new alkaloids, norpandamarilactonine-A and -B, from Pandanus amaryllifolius by spectroscopic and synthetic methods

Two new alkaloids, norpandamarilactonine-A (1) and -B (2), which have a pyrrolidinyl-alpha,beta-unsaturated gamma-lactone moiety as in the known pandamarilactonine alkaloids, were isolated from the leaves of Pandanus amaryllifolius. Their structures were determined by spectroscopic analysis and total synthesis.     

Dulxanthones F-H, three new pyranoxanthones from Garcinia dulcis

Three new pyranoxanthones, dulxanthones F-H (1-3), have been isolated from the leaves of Garcinia dulcis. Their structures have been determined on the basis of extensive NMR studies.     

Cytotoxic and anti-inflammatory triterpenoids from Toona ciliata

Toonaciliatavarins A-H (1-8), including three new protolimonoids (1-3), two new tirucallane-type triterpenoids (4 and 5), and three new tetranortriterpenoids (6-8), and 10 known compounds were isolated from the stem barks of Toona ciliata Roem. var. henryi. Their structures were identified on the basis of spectroscopic analysis. The absolute configurations of 2 and 8 were determined by ECD calculation. The new isolates were evaluated for their cytotoxicities using six human cancer cell lines and also for their inhibitory effects on lipopolysaccharide-induced nitric oxide production in RAW264.7 cells. Compounds 4 and 5 showed moderate cytotoxicities, and the protolimonoids (1-3) exhibited marked inhibitory effects on LPS-stimulated NO production.