银屑病患者血浆和皮损纤维连接蛋白含量测定及分子降解状态的研究

为了探讨银屑病患者血浆纤维连接蛋白（ＰＦＮ）和皮损可溶性纤维连接蛋白（ＳＦＮ）的存在状态，采用火箭电泳法和蛋白印迹法分别对９０例银屑病ＰＦＮ和２０例银屑病斑块状皮损ＳＦＮ进行检测。结果表明，患者组ＰＦＮ水平显著低于正常人对照组，皮损ＳＦＮ含量明显高于正常人皮肤。扫描结果提示，患者组ＰＦＮ降解增强可能是ＰＦＮ减少的主要原因，不同期银屑病组ＰＦＮ降解程度有差异。皮损ＳＦＮ增多可能是由于局部合成增加及ＰＦＮ向表皮渗漏所致。动态观察银屑病患者ＰＦＮ分子降解状态，具有重要的临床意义     

银屑病患者血浆和皮损纤维连接蛋白含量测定及分子降解状态的…

为了探讨了银屑病患者血浆纤维连接蛋白和皮损可溶性纤维连接蛋白的存在状态，采用火箭电泳法和蛋白印迹法分别对９０例银屑病ＰＦＮ和２０例银屑病斑块状皮损ＳＦＮ进行检测。结果表明，患者组ＰＦＮ水平显著低于正常人对照组，皮损ＳＦＮ含量高于正常人皮肤、扫描结果提示。患者组ＰＦＮ降解增强可能是ＰＦＮ减少的主要原因。不同期银屑病组ＰＦＮ降解程度有差异，皮损ＳＦＮ增多可能是由于局部合成增加及ＰＦＮ向表皮渗漏所致，动     

原癌基因c-fos及c-jun在银屑病皮损中的异常表达

目的为了探索ｃ ｆｏｓ、ｃ ｊｕｎ与银屑病的关系。方法运用原位杂交技术并通过图像定量分析,对３６例寻常性银屑病患者及１５例正常人皮肤中ｃ ｆｏｓ及ｃ ｊｕｎ的表达进行观察。结果银屑病进行期皮损中,ｃ ｆｏｓ、ｃ ｊｕｎ表达全层减少（Ｐ＜０．０１）,恢复期皮损中ｃ ｆｏｓ、ｃ ｊｕｎ的表达与正常皮肤差异无显著性意义Ｐ＞０．０５）。结论提示ｃ ｆｏｓ、ｃ ｊｕｎ可能与银屑病角朊细胞分化受阻及表现型异常密切有关。     

斑块状银屑病皮损部位末梢血和肘正中静脉血中相关炎症因子含量的检测

目的 检测参与银屑病发病的相关炎症因子在皮损部位末梢血（以下简称皮损血）及肘正中静脉血（以下简称静脉血）中的含量情况.方法 34例斑块状银屑病患者,分别采集皮损血和静脉血,用双抗体夹心酶联免疫吸附法（ELISA法）分别检测并比较肿瘤坏死因子（TNF-α）、白细胞介素-8（IL-8）、P物质（SP）、银屑病p27抗原（Pso p27）在皮损血和静脉血中的含量.结果 皮损血中TNF-α、IL-8、Pso p27的含量明显高于静脉血中的含量,差异有统计学意义（P＜0.05）;而皮损血中SP的含量低于静脉血中SP的含量,差异有统计学意义（P=0.002）.结论 部分炎症因子在斑块状银屑病患者皮损血中的含量高于其在静脉血中的含量,提示炎症因子在皮损部位浓集可能是银屑病发病的重要因素.     

银屑病患者血浆内皮素含量检测

为了探讨内皮素与银屑病的关系及其在发病机理中的作用，采用放射免疫分析法检测了３２例正常人及５４例银屑病患者血浆内皮素的变化，其中寻常型银屑病４５例（进行期３２例，静止期１３例），红皮病型银屑病９例。结果显示：正常人血浆内皮素水平为４３．５±１５．７１ｐｇ／ｍｌ，银屑病患者血浆内皮素含量为６２．０１±２１．１５ｐｇ／ｍｌ，较正常对照组显著增高（Ｐ＜０．０１），红皮病型又较寻常型患者的血浆内皮素水平为高（Ｐ＜０．０５）。寻常型银屑病患者中，进行期与静止期相比其内皮素水平虽有增高，但无显著性差异（Ｐ＞０．０５）。本研究提示内皮素可能与银屑病的发病有关，进一步了解其作用机理，可能对银屑病的防治具有重要意义     

银屑病患者皮瓣鳞屑真菌的分离及流式细胞计数分析

目的 了解真菌特别是糠秕孢子菌与银屑病的关系。方法 对８２例银屑病患者和４０例正常人的头皮，指甲，背部，上肢或下肢４个部位的皮肤鳞屑在３种不同培养基上进行了真菌培养分离，并应用流式细胞计数检测了８株银屑病皮损处糠秕虫孢子菌和４株正常人的糠秕孢子菌的ＤＮＡ总含量及周期变化，结果 （１）发现银屑病患者的真菌率明显高于对照组（Ｐ〈０．０５），银屑病患者皮损处真攻阳性率与非皮损处的阳生率有非常显著性差异（     

红斑、丘疹及鳞屑性皮肤病

971414 寻常性银屑病患者血浆肿瘤坏死因子水平检测/王惠芳…//中国皮肤性病学杂志.-1996,10（6）.-327 检测40例寻常性银屑病患者血浆中肿瘤坏死因子（TNF）含量及IgG、IgA、IgM与C_3水平,以探讨TNF在银屑病发病中的意义。对照组为43例正常人。结果:银屑病组血浆TNF较正常对照组显著升高。男性高于女性（P<0.05）,进行期高于静止期,伴甲部损害者TNF明显升高。TNF水平与病情严重程度呈正相关,与IgA、IgG、IgM、C_3无明显线性关系。本实验结果证实银屑病患者TNF血浆含量高于正常人46倍。40例中血浆TNF最低值均明显高于正常人,提示TNF参与了银屑病的病理生理过程。而TNF含量高低与疾病预后有一定关系。（刘辅仁）971415 银屑病患者皮损和正常皮肤中Ig和C_3的免     

银屑病患者角质形成细胞对T淋巴细胞CD25、CD69表达的影响

目的 探讨银屑病患者角质形成细胞（KC）对T淋巴细胞CD25、CD69表达的影响。方法 分离10例银屑病患者KC，密度梯度离心法分离单一核细胞（PBMC）；流式细胞仪检测混合培养后T细胞活化标志CD25、CD69的表达。结果 银屑病患者皮损KC作用的自体外周血T细胞CD25、CD69表达水平分别与非皮损KC作用组及自体T细胞自然增殖组相比显著增高，银屑病非皮损KC+自体T细胞共培养组与自体T细胞自然增殖组相比，差异无统计学意义。银屑病皮损、非皮损KC作用的正常人T细胞CD25、CD69表达均显著高于正常人外周血T细胞自然增殖组，银屑病皮损KC+正常人T细胞共培养组与非皮损组相比，差异无统计学意义。结论 银屑病患者局部存在慢性炎症反应，可能是由于皮损KC免疫表型发生改变从而作为自身抗原，启动自身免疫反应。     

银屑病皮损和正常人皮肤五种细胞外基质成分的免疫组化检测

用 ＰＡＰ法显示１８例银屑病皮损和 ７例健康人皮肤Ⅰ、Ⅲ、Ⅳ 型胶原蛋白（简写为Ⅰｃ、Ⅲｃ、Ⅳｃ）、纤维连接蛋白（ＦＮ）和基膜连接蛋白（ＬＮ）的分布。在正常人皮肤中 Ｉｃ和Ⅲｃ分布于真皮层，而在银屑病皮损中Ｉｃ、Ⅲｃ显色较正常人皮肤深，在真皮浅层、血管周围及炎细胞浸润区呈局灶性浅染。正常人皮肤中Ⅳｃ、 ＦＮ和 ＬＮ呈线状分布于基底膜，在银屑病皮损中则呈不规则节段样增粗和缺损； ＦＮ和ＬＮ在乳头顶端明显缺损，其两边呈网状增厚。此结果可能与成纤维细胞和角朊细胞的合成异常增强有关； Ｉｃ和Ⅲｃ的区域性浅染及基底膜成分在乳头顶端的缺损则可能与炎症细胞的吞噬及释放的蛋白水解酶的破坏有关。     

银屑病患者治疗前后表皮细胞中热休克蛋白60的表达研究

为了探讨热休克蛋白60与银屑病的关系，采用LSAB免疫组织化学法，检测16例银屑病皮损、非皮损及治愈后表皮细胞中和6例正常人对照组表皮细胞中HSP60的表达。结果发现HSP60在正常人及银屑病患者非皮损、治愈后表皮细胞中呈阴性表达，在银屑病皮损中表达增强，提示HSP60在银屑病应激保护机制中可能发挥着重要作用。     

不同病期银屑病皮损组织CK20、S100A7、SP的表达及其相关性

目的 探讨不同病期银屑病皮损组织中CK20、S100A7、SP的表达及CK20与S100A7、SP表达的关系.方法 选择经过非正规治疗,新旧皮损同时存在的患者19例,获取皮损旁的正常皮肤组织(发病前期)、皮损组织(进展期)和皮损修复后的病灶皮肤组织(缓解期),观察皮损中免疫组化CK20、S100A7、SP的表达情况.结果 免疫组化图像分析各组A值,发病前期组、进展期组和缓解组CK20分别为7683.80±6134.55、18305.04±13171.30、7257.53±4417.75.S100A7分别为8789.05±6240.91、18058.01±16537.18、9295.65±9310.02.SP分另为3242.51±3775.41、9364.98±7596.64、2910.85±3349.46.进展期皮损组织中CK20与S100A7,SP表达相对于发病前期和缓解期明显增加其差异均有统计学意义,P＜0.05.发病前期组与缓解组其差异均无统计学意义,P＞0.05.CK20与S100A7、SP表达呈正相关,相关系数R分别为0.779、0.876、P＜0.05.结论 银屑病发病与Merkel细胞数量的变化有一定的关系.     

The Increased Expression of Matrix Metalloproteinase-9 Messenger RNA in the Non-lesional Skin of Patients with Large Plaque Psoriasis Vulgaris

Background

A difference of the interleukin-18 (IL-18) mRNA expression among several proinflammatory genes was previously observed between large plaque (LP) psoriasis patients (more than 5 cm lesions are typical) and small plaque (SP) psoriasis patients (1~2 cm lesions are typical). Therefore, it is necessary to test whether there is any difference in the expression of the genes that activate IL-18 or the expression of genes that are induced by IL-18.

Objective

To test the differential mRNA expressions of caspase-1, STAT-6, MMP-1, MMP-2, MMP-9 and TIMP-1 according to the clinical types of psoriasis vulgaris lesions in Korean patients, we have analyzed the skin samples of psoriasis vulgaris patients.

Methods

The total cellular RNA of skin samples from groups of patient with LP and SP psoriasis was analyzed by performing real-time PCR (the Taqman method) to compare the differences in the mRNA expressions.

Results

The caspase-1 and STAT-6 mRNA expression levels from the SP lesional skin of the patients were increased compared with the caspase-1 and STAT-6 mRNA expression levels from SP non-lesional skin or normal skin, but these expression levels from the SP non-lesional skin were not significantly different from those of the LP non-lesional skin. Among MMP-1, MMP-2, MMP-9 and TIMP-1, the expressions of MMP-1, MMP-2 and MMP-9 mRNA were increased in the SP lesional skin compared with those of the SP non-lesional skin. The MMP-1 mRNA expressions in both the LP and SP lesional skin were increased compared with those in the normal skin (p=0.028 and p=0.007 respectively). The MMP-9 mRNA expression in the LP non-lesional skin was elevated compared with the MMP-9 mRNA expression in the SP non-lesional skin (p=0.047). The TIMP-1 mRNA expression levels from the non-lesional skin and the lesional skin of the psoriasis patients and the normal skin samples were not significantly different.

Conclusion

The increased expression of MMP-9 mRNA in the LP non-lesional skin compared to that of the SP non-lesional skin in the psoriatic skin suggests that the increased MMP-9 mRNA expression is related to the large size type of lesion.     

性激素与尖锐湿疣人乳头瘤病毒的相关性研究

为了探讨尖锐湿疣人乳头瘤病毒感染与性激素的关系，采用斑点杂交、免疫组化和放免测定等技术，检测了重庆地区４７例女性尖锐湿疣（ＣＡ）患者病变组织中ＨＰＶＤＮＡ、雌激素受体（ＥＲ）以及血清中雌二醇（Ｅ２）、孕酮（Ｐ）和睾酮（Ｔ）。结果提示：妊娠期ＨＰＶＤＮＡ含量、ＥＲ表达量均明显高于对照组和其余各组（Ｐ＜０．００１），健康组中无ＥＲ表达；ＨＰＶＤＮＡ含量与ＥＲ表达量之间呈正相关（Ｐ＜０．００１），与血清Ｅ２、Ｐ也呈正相关（Ｐ＜０．００１），与Ｔ无明显相关（Ｐ＞０．０５）。我们认为性激素特别是雌激素、孕激素在ＨＰＶ感染中可能有协同作用     

Plasma concentration of selected neuropeptides in patients suffering from psoriasis

The aim of this study was to evaluate plasma levels of substance P (SP), calcitonin gene-related peptide (CGRP), vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) during psoriasis course. METHODS: Seventy-three patients with psoriasis and 32 healthy volunteers were included. Detailed demographic and disease anamnesis was obtained from every patient. The disease severity was assessed using the Psoriasis Area and Severity Index score. Plasma levels of SP, CGRP, VIP and NPY were measured radioimmunologically. RESULTS: Plasma levels of SP and NPY did not significantly differ between patients with psoriasis and controls (median SP: 52.8 and 57.9 pg/ml, respectively; P = 0.32; median NPY: 8.5 and 8.2 pg/ml, respectively; P = 0.67). CGRP plasma concentration was significantly elevated in psoriatic individuals both before (median 43.1 pg/ml) and after treatment (median 45.4 pg/ml), in comparison with healthy donors (median 13.5 pg/ml; P < 0.01 and P = 0.03, respectively). Treatment did not significantly influence plasma CGRP levels (P = 0.3). Median VIP plasma concentration in psoriatics before treatment was significantly higher compared with healthy controls (medians 66.9 and 60.1 pg/ml, respectively; P = 0.04), but the therapy resulted in significant decrease in VIP plasma level (median 19.0 pg/ml; P < 0.001). In psoriatic patients significant correlations were noted between NPY and VIP (R = 0.34; P < 0.01), and VIP and CGRP plasma levels, both before (R = 0.28; P = 0.03) and after the treatment (R = 0.44; P < 0.01). CONCLUSIONS: Based on our results and previous literature data it could be suggested that neuropeptides may be involved in the development of psoriatic lesions.     

亚急性湿疹和银屑病患者皮损中半胱氨酰白三烯受体的表达

目的探讨湿疹和银屑病患者皮损中半胱氨酰白三烯受体CysLTR1和CysLTR2的临床意义。方法免疫组化SP法分别检测10例亚急性湿疹和20例寻常性银屑病患者皮损中CysLTR1和CysLTR2的表达,并以外科手术中相应部位皮肤组织作为对照。结果银屑病与湿疹患者皮损中CysLTR1和CysLTR2的表达显著高于正常对照,其阳性细胞主要位于表皮、皮脂腺、毛囊、汗腺、平滑肌及血管壁等;3组中CysLTR1和CysLTR2两型受体间的分布及表达强弱差异无统计学意义。结论人皮肤组织中同时存在CysLTR1和CysLTR2的表达,银屑病与湿疹患者皮损中CysLTR1和CysLTR2的表达增强。     

Neutrophil zinc levels in psoriasis and seborrhoeic dermatitis

The median zinc content of neutrophils was significantly reduced in 16 patients with psoriasis in comparison to both normal controls and six patients with seborrhoeic dermatitis (P less than 0.05). This reduction was unrelated to the extent of skin involvement. Plasma and erythrocyte zinc levels were unchanged.     

寻常性银屑病皮损中SP,NK-1R和IL-23 p19基因的表达

目的探讨银屑病皮损组织中P物质(SP),SP受体(NK-1R)和IL-23 p19基因实时定量表达的意义。方法收集45例银屑病患者(PASI评分<12分的25例,PASI评分≥12分)与20名正常对照者的临床资料,采用Real-time PCR检测患者和对照组的SP,NK-1R和IL-23 p19的mRNA定量表达水平。结果SP,NK-1R和IL-23 p19mRNA在正常对照组、银屑病PASI(12组和PASI≥12组中的相对表达量均明显增高,差异有显著性(P<0.001)。相关分析显示,IL-23p19 mRNA在各组的表达与SP,NK-1R的表达呈正相关(r分别为0.867和0.846,P<0.001)。结论SP,NK-1R,IL-23p19可能参与银屑病的发病,并与银屑病严重程度相关。     

Prolactin level is significantly elevated in lesional skin of patients with psoriasis

Background Accumulating data point to a potential role of prolactin in the pathogenesis of psoriasis. Methods We initiated a study including psoriasis patients (n = 15) and healthy volunteers (n = 15) as controls. Psoriasis area and severity index (PASI) score was evaluated, and prolactin levels in serum and blister fluid were assessed by enzyme‐linked immunosorbent assay (ELISA). Results Prolactin levels were significantly (P < 0.01) elevated in blister fluid of psoriatic lesional skin. Correlations between PASI score and different serum prolactin levels in lesional and non‐lesional skin were insignificant. Significant positive correlations of prolactin level were observed between lesional and non‐lesional skin in psoriasis (P < 0.05) and between serum and clinically normal skin in both psoriasis and control subjects (P < 0.05). Conclusions Locally produced prolactin may be involved in the pathogenesis of psoriatic lesions.     

磷酸化c-Jun氨基末端激酶和P38丝裂原活化蛋白激酶在寻常性银屑病皮损中的表达

目的 探讨磷酸化c-Jun氨基末端激酶（p-JNK）和P38丝裂原活化蛋白激酶（p-P38MAPK）在寻常性银屑病皮损中的表达。 方法 收集30例确诊的寻常性银屑病患者皮损,同时收集30例健康人皮肤组织作为对照组。采用免疫组化及Western印迹法分别检测p-JNK和p-P38MAPK蛋白在寻常性银屑病皮损组织和正常人皮肤中的表达情况。 结果 免疫组化结果显示,寻常性银屑病皮损组织p-JNK和p-P38MAPK表达的平均吸光度（AOD）值分别为0.663 ± 0.016和0.436 ± 0.011,均明显高于对照组（0.333 ± 0.009和0.306 ± 0.010）,差异有统计学意义（t = 44.869、21.913,均P < 0.001）。Western印迹法同样显示,寻常性银屑病皮损p-JNK和p-P38MAPK蛋白相对表达量均显著高于对照组,差异均有统计学意义（t值分别为20.477和165.084,均P < 0.05）。结论 JNK和P38MAPK的活化可能参与了寻常性银屑病表皮细胞的过度增殖。     

Substance P is diminished and vasoactive intestinal peptide is augmented in psoriatic lesions and these peptides exert disparate effects on the proliferation of cultured human keratinocytes.

An involvement of neurogenic components in the pathogenesis of psoriatic lesions has been suggested and neuropeptides are thought to play a modulatory role in cutaneous inflammation. In this study, we evaluated the immunoreactivity of the neuropeptides vasoactive intestinal polypeptide (VIP) and substance P (SP) in the skin of patients with chronic plaque psoriasis, by immunohistochemistry and radioimmunoassay. No differences were observed, by immunohistochemistry, in the expression and localization of VIP and SP between psoriatic and normal skin. Using the radioimmunologic technique on whole skin homogenates, VIP levels were significantly increased in psoriatic lesions as compared to normal skin. By contrast, SP levels were significantly lower in lesional and non-lesional psoriatic skin than in normal skin. In addition, we examined the effect of VIP and SP on the proliferation of cultured normal human keratinocytes. VIP (1-28) (1 nM-1 microM) as well as VIP fragments (10-28) (1 nM-1 microM) and (22-28) (1 nM-1 microM) stimulated the proliferation of keratinocytes in a dose-dependent manner, whereas the VIP fragment (1-12) (1 nM-1 microM) was ineffective. The VIP antagonist (N-Ac-Tyr1, D-Phe2)-GRF (1-29)-NH2 (0.1 microM) significantly inhibited the VIP effect on keratinocytes. On the other hand, SP (0.1 microM) not only failed to stimulate keratinocyte growth, but also blocked the VIP-induced stimulation of these cells. The imbalance of cutaneous VIP and SP and their disparate effects on the proliferation of normal human keratinocytes in culture would suggest that these peptides are involved in the pathogenesis of psoriasis and may exert different modulatory activities in the mechanisms underlying the psoriatic lesion.