孕激素受体膜成分1在子宫内膜癌中表达及对细胞增殖的影响

目的:检测子宫内膜癌组织中孕激素受体膜成分1(PGRMC1)的蛋白表达特点,分析其临床意义;并探讨PGRMC1对子宫内膜癌细胞增殖调控的影响。方法:采用蛋白质免疫印迹法(Western blot)检测70例子宫内膜癌组织及28例对照子宫内膜组织中PGRMC1蛋白表达水平;应用细胞免疫组化方法检测三种人子宫内膜癌细胞系中PGRMC1的表达定位;将PGRMC1的小分子抑制剂Ag205以不同浓度分别刺激人子宫内膜癌细胞Ishikawa、Hec-1A、KLE 72小时,应用CCK-8(cell counting kit 8)方法检测Ag205对细胞活力的影响。结果:子宫内膜癌组织中PGRMC1表达较正常内膜显著升高(P=0.038);PGRMC1在各期子宫内膜癌组织中的表达无差异,但在Ⅰ期患者中,PGRMC1在Ⅰa期表达较Ⅰb期显著高(P=0.019);PGRMC1表达与子宫内膜癌组织中ER表达水平相关(P=0.015),但与分化程度、淋巴结转移及脉管癌栓无关,与PR、p53、Ki-67及bcl-2表达亦无显著相关性;20μM Ag205显著抑制三种子宫内膜癌细胞的增殖活力,其中以KLE细胞的抑制作用最明显,从最低浓度(5μM)即出现抑制效应,并随浓度增加抑制作用逐步增强。结论:子宫内膜癌内膜中PGRMC1表达显著增高;增高的PGRMC1参与调控子宫内膜癌细胞的增殖活性。     

MTA-1、HIF-1α、ER、PR在子宫内膜癌临床特征上的表达差异及相关性分析

目的 探究肿瘤转移相关基因-1(MTA-1)、缺氧诱导因子-1α(HIF-1α)、雌激素受体(ER)、孕激素受体(PR)在子宫内膜癌(EC)临床特征上的表达差异及相关性。方法 选取2017年6月至2020年6月保定市妇幼保健院收治的62例EC患者作为研究对象,设为研究组,获取其的病理标本。另选取同期于保定市妇幼保健院进行治疗的118例子宫肌瘤、子宫腺肌症、子宫内膜息肉等患者设为对照组,均行子宫全切术治疗并获取子宫内膜组织标本,其中56例为子宫内膜不典型增生患者,设为对照1组,60例为正常子宫内膜患者,设为对照2组。对纳入标本进行免疫组化检测,观察并比较MTA-1、HIF-1α、ER、PR阳性表达情况,分析MTA-1、HIF-1α、ER、PR在EC临床特征上的表达差异及相关性。结果 研究组MTA-1和HIF-1α阳性率高于对照组,对照2组高于对照1组(P<0.05);研究组ER和PR阳性率低于对照组,对照2组低于对照1组(P<0.05);EC患者在不同肌肉浸润程度、不同国际妇产科联盟(FIGO)分期、不同组织学分级及有无淋巴结转移中MTA-1、HIF-1α、ER、PR阳性率表达情况比较,差异具有统计学意义(P<0.05);MTA-1、HIF-1α阳性表达与肌肉浸润程度、FIGO分期及淋巴结转移呈正相关(r=0.316、0.254、0.347,0.376、0.412、0.269,P<0.05),与组织学分级呈负相关(r=-0.562、-0.447,P<0.05),ER、PR阳性表达与上述4个临床特征均呈负相关(r=-0.485、-0.226、-0.634、-0.215,-0.313、-0.664、-0.415、-0.532,P<0.05)。结论 MTA-1、HIF-1α在EC中的阳性表达率升高,ER和PR阳性表达率降低,MTA-1、HIF-1α、ER、PR与临床特征关系密切,具有一定相关性,上述指标的检测可对临床诊断和治疗EC患者提供有效依据。     

子宫内膜样癌及卵巢浆液性癌中PR、P53、ER的表达及其临床病理学意义

目的:探究与分析传统子宫内膜样癌及卵巢浆液性癌中PR、P53、ER的表达及其临床病理学意义。方法:选取我院自2013年1月至2014年10月收治的120例子宫内膜样癌及卵巢浆液性癌患者作为研究对象进行回顾性分析,其中子宫内膜样癌患者45例,卵巢浆液性癌患者75例。采用免疫组化En Vision法分析所选的患者病理标本中PR、P53、ER的表达情况。对比在不同病理分型、组织分级、临床分期中PR、P53、ER的表达水平。结果:子宫内膜样癌PR、P53、ER的表达率与浆液性癌PR、P53、ER的表达率比较,组间差异显著(χ~2=5.49,P0.05;χ~2=4.76,P0.05;χ~2=4.23,P0.05)。中、高分化癌PR、P53、ER的表达率与低分化癌PR、P53、ER的表达率相比,组间差异显著(χ~2=5.33,P0.05;χ~2=4.23,P0.05;χ~2=4.57,P0.05)。临床分期Ⅰ~Ⅱ期患者PR、P53、ER的表达率分别与低分化癌PR、P53、ER的表达率相比,组间对比差异显著(χ~2=5.33,P0.05;χ~2=4.23,P0.05;χ~2=4.57,P0.05)。结论:子宫内膜样癌及卵巢浆液性癌中PR、P53、ER的表达存在较大差异,这对于卵巢癌病变诊断及鉴别诊断具有重要意义,值得在临床中推广PR、P53、ER的监测。     

探讨绝经前子宫内膜癌患者免疫组化及病理特征

目的探讨绝经前子宫内膜癌患者免疫组化及病理特征。方法对佛山市顺德区北滘医院2015年1月至2017年1月收治的80例子宫内膜癌患者的临床资料进行回顾性分析。结果绝经前组患者病理分期ⅠA期比例显著高于绝经后组,差异具有统计学意义(P 0. 05),ⅠB期比例显著低于绝经后组,差异具有统计学意义(P 0. 05),病理类型子宫内膜样腺癌比例显著高于绝经后组,差异具有统计学意义(P 0. 05),ER+(0-50%)比例显著低于绝经后组,差异具有统计学意义(P 0. 05),ER+( 50%)比例显著高于绝经后组,差异具有统计学意义(P 0. 05),PR-、PR+(0-50%)比例均显著低于绝经后组,差异具有统计学意义(P 0. 05),PR+( 50%)比例显著高于绝经后组,差异具有统计学意义(P 0. 05),ER、PR+( 50%)比例显著高于绝经后组,差异具有统计学意义(P 0. 05)。两组病理分期Ⅰ-Ⅱ期患者的免疫组化ER+( 50%)比例显著高于Ⅲ-Ⅳ期患者,差异具有统计学意义(P 0. 05),PR-比例显著低于Ⅲ-Ⅳ期患者(P 0. 05),+( 50%)比例显著高于Ⅲ-Ⅳ期患者,差异具有统计学意义(P 0. 05)。结论绝经前子宫内膜癌患者免疫组化ER、PR具有较高的阳性率,病理类型主要为子宫内膜样腺癌,癌细胞具有较高的分化程度。     

子宫内膜癌中错配修复蛋白与ER、PR、p53表达的临床病理意义及其对预后的影响

目的 通过分析错配修复(mismatch repair, MMR)蛋白与ER、PR及p53在子宫内膜癌中的表达情况,探讨其临床病理意义及其对预后的影响。方法 收集2017年1月至2020年10月在十堰市太和医院确诊的226例子宫内膜癌患者的临床病理资料,采用免疫组织化学法对肿瘤组织进行MMR蛋白、ER、PR及p53检测,分析其表达与临床病理的关系及其对患者预后的影响。结果 226例子宫内膜癌组织中MMR蛋白的缺失率为18.6%(42/226),ER及PR的阴性表达率分别为6.6%(15/226)、11.9%(27/226),p53突变型表达率为59.7%(135/226)。MMR蛋白缺失在肿瘤分级、肌层浸润深度、淋巴脉管浸润(LVSI)及术后辅助治疗中差异具有统计学意义(P<0.05)。p53突变型表达多为非子宫内膜样腺癌,且多为高级别子宫内膜癌(P<0.05)。ER与PR的表达呈正相关,ER阴性表达与组织学类型、肿瘤分级、术后辅助治疗相关(P<0.01),而PR阴性表达还与国际妇产科联盟(FIGO)分期、LVSI及淋巴结转移有关(P<0.05)。p53突变型在...     

性传播疾病

20072614PCNA、p53、Ki-67在尖锐湿疣中的表达和意义/林友胜(四川省卫生干部管理学院),张玉梅∥四川医学.-2007,28(3).-322~323运用免疫组化法检测PCNA、p53、Ki-67在尖锐湿疣(CA)中的表达,结果PCNA在CA组阳性表达率与正常对照组比较差异无统计学意义(P>0.05),但CA组PCNA强阳性率显著高于对照组(P<0.05)。p53在CA组阳性表达率明显高于正常对照组,差异具有统计学意义(P<0.05)。Ki-67在CA组阳性表达率与正常对照组差异无统计学意义(P>0.05),但CA组Ki-67强阳性率也显著高于对照组(P<0.05)。表3参8(惠海英)20072615CD105和p63在…     

表皮生长因子受体、Ki-67在外阴营养不良组织中的表达

目的 探讨表皮生长因子受体（EGFR）、增殖细胞核抗原Ki-67 在外阴营养不良组织中的表达及其临床意义。方法 免疫组化法对160例外阴营养不良患者皮损中的EGFR、Ki-67表达进行检测,比较EGFR、Ki-67在外阴营养不良各病理类型中表达的差异,并分析其与临床病理特征的相关性。结果 EGFR、Ki-67在外阴营养不良鳞状细胞增生型、硬化苔藓型、混合型中的表达差异无统计学意义（P ＞ 0.05）;EGFR与Ki-67的表达呈正相关,r = 0.66,P ＜ 0.05;EGFR、Ki-67的阳性表达率在不同发病年龄组差异无统计学意义（P > 0.05）,在不同病程组差异有统计学意义（P ＜ 0.05）。结论 EGFR、Ki-67阳性表达率在不同病程组有差异。     

异常子宫出血与雌孕激素及其受体水平关系的探讨

目的:探讨雌孕激素及其受体水平与子宫异常出血的关系。方法:随机选取门诊异常子宫出血患者,采用化学发光免疫分析仪及试剂盒进行激素水平测定,同时行分段诊刮送病理检查及雌孕激素受体检测。结果:子宫内膜癌、子宫内膜不典型增生、子宫肌瘤、子宫内膜息肉患者E2含量明显高于对照组(P<0.05),子宫肌瘤及有排卵型功血患者P含量与对照组相比差异显著(P<0.05,P<0.01),除有排卵型功血外,其他各种类型子宫异常出血患者的子宫内膜中ER、PR阳性率均明显高于对照组,差异显著(P<0.05,P<0.01及P<0.005)。结论:子宫异常出血与E2、P水平及子宫内膜中ER、PR含量有明显相关性。     

程序性细胞死亡因子5在子宫内膜癌患者中的表达分析

目的分析程序性细胞死亡因子5(PDCD5)表达在子宫内膜癌诊断中的应用价值。方法选取2017年1月至2018年12月南方医科大学附属齐齐哈尔市第一医院诊治的53例子宫内膜癌患者作为研究对象。将这53例患者设为研究组,并选择同期在南方医科大学附属齐齐哈尔市第一医院接受手术治疗的53例正常增生期子宫内膜组织患者设为对照组。两组患者均行免疫组化方法检测,对比两组患者PDCD5蛋白阳性表达率、PDCD5 mRNA表达量。结果 PDCD5蛋白阳性表达率、PDCD5 mRNA表达量比较,研究组患者[(45.28%)和(0.69±0.25)]均显著低于对照组患者[(81.13%)和(1.14±0.30)],组间差异均具有统计学意义(均P0.05),且子宫内膜癌细胞中的PDCD5表达显著下调。结论正常增生期子宫内膜及子宫内膜癌组织中的PDCD5蛋白阳性表达差异显著,在子宫内膜癌细胞中的PDCD5表达显著下调,提示PDCD5表达情况可作为预测子宫内膜癌发生和发展的重要指标。     

体重指数、胰岛素抵抗在子宫内膜癌中的表达及其与预后的关系

目的研究体重指数(BMI)及胰岛素抵抗(IR)在子宫内膜癌患者中的表达及其与预后的关系。方法选取2016年3月至2018年3月蓬莱市人民医院诊治的100例子宫内膜癌患者作为研究对象。将这100例患者设为观察组,另选取同期进行体检的健康妇女100例设为对照组。分别对两组研究对象BMI、空腹血糖(FPG)、空腹胰岛素(FINS)水平进行检测。计算两组研究对象HOMA-IR,并分析BMI、HOMA-IR与预后的关系。结果观察组患者的BMI明显高于对照组研究对象,差异具有统计学意义(P0.05)。观察组患者的HOMA-IR明显高于对照组研究对象,差异具有统计学意义(P0.05)。随病情发展,患者BMI及HOMA-IR逐渐升高。Ⅲ期和Ⅳ期子宫内膜癌患者的BMI及HOMA-IR显著高于Ⅰ期和Ⅱ期子宫内膜癌患者,差异均具有统计学意义(均P0.05)。多因素Logistic回归分析显示,BMI及HOMA-IR均为影响子宫内膜癌患者预后的危险因素,差异具有统计学意义(P0.05)。结论子宫内膜癌患者的BMI及HOMA-IR普遍高于健康人,两者均为子宫内膜癌预后的危险因素,可影响疾病预后发展。     

EGFR通路在相关皮肤病发病机制中的研究进展

表皮生长因子受体（EGFR）是一种受体酪氨酸激酶，存在于几乎所有上皮细胞和多种间充质细胞的细胞膜上，EGFR通路的失调可能导致细胞增殖、血管生成、细胞凋亡受损、细胞侵袭性增强，影响银屑病、特应性皮炎等炎症性皮肤病和皮肤鳞状细胞癌、黑素瘤等肿瘤性皮肤病的发生与发展，本文综述了EGFR通路在相关皮肤病发病机制中的研究进展。     

Assessment of gene expression levels of proopiomelanocortin (POMC) and melanocortin‐1 receptor (MC1R) in vitiligo

Proopiomelanocortin (POMC) and melanocortin 1 receptor (MC1R) are regulators of melanogenesis and pigmentation. Our objective was to estimate their levels, searching for a possible role of the melanocortin system in vitiligo. This study included 40 vitiligo patients and 40 controls. Skin biopsies were taken from lesional and non‐lesional skin of patients and from the non‐sun exposed skin of controls to detect the expression of POMC and MC1R using quantitative real‐time polymerase chain reaction. Both factors were significantly lower in lesional than non‐lesional skin and controls, while they were significantly higher in non‐lesional skin than in controls. There was a statistically significant positive correlation between lesional levels of POMC and MC1R, as well as between non‐lesional levels of POMC and MC1R in the patients. On the other hand, we found a statistically significant negative correlation between the lesional and non‐lesional levels of POMC, as well as between the lesional and non‐lesional levels of MC1R in the patients. As a conclusion, the melanocortin system could play a role in the pathogenesis of vitiligo or could be affected as the end result of the disease.     

可溶性Toll样受体2联合PCT检测对血流感染致脓毒症诊断价值分析

目的探讨可溶性Toll样受体2(sTLR-2)联合降钙素原(PCT)检测对血流感染致脓毒症诊断价值。方法选取2019年3月—2021年3月西安交通大学第二附属医院收治的112例血流感染致脓毒症患者为研究对象,统计血流感染致脓毒症患者病原菌分布情况;分析影响血流感染致脓毒症患者病原菌感染特征的因素;分析sTLR-2联合PCT检测对血流感染致脓毒症患者的诊断价值。结果112例血流感染致脓毒症患者中有81例(72.32%)感染革兰阴性菌,有31例(27.68%)感染革兰阳性菌。Logistic回归多因素分析显示PCT(OR:4.035,95%CI:1.660~9.806)、sTLR-2(OR:3.904,95%CI:1.606~9.488)是影响血流感染致脓毒症患者病原菌感染类型的独立因素(P<0.05)。ROC曲线显示PCT、sTLR-2及二者联合诊断革兰阴性菌血流感染所致脓毒症的AUC分别为0.755、0.753、0.885(95%CI分别为:0.655~0.856,0.642~0.864,0.800~0.949)。结论革兰阴性菌血流感染致脓毒症发生风险高,PCT、sTLR-2与血流感染致脓毒症患者病原菌感染类型有关,二者联合预测革兰阴性菌血流感染所致脓毒症具有较高效能。     

Double-stranded RNA induces melanocyte death via activation of Toll-like receptor 3

As cutaneous pigment-producing cells, melanocytes can become targets of primary and secondary immune response as can be seen in diseases like vitiligo and Vogt-Koyanagi-Harada (VKH) syndrome. Viral infections have previously been implicated as a possible precipitating factor in the destruction of melanocytes in these disorders. During viral replication, double-stranded RNA (dsRNA) is produced as an intermediate metabolite, which induces antiviral and inflammatory responses through Toll-like receptor 3 (TLR3) in cells of innate immune system. The functional responses of melanocytes to dsRNA, however, remain unclear. Herein, we demonstrated that human melanocytes expressed TLR3 at a constitutive and inducible level. Stimulation with poly(I:C), a synthetic dsRNA analogue, triggered apoptosis of melanocytes. The apoptosis-inducing effect was shown by RNA interference to be largely dependent on TLR3, but occurred independently of NF-κB activation since treatment with specific NF-κB inhibitor Bay 11-7082 failed to prevent the process. In contrast, IFN-β neutralizing Ab blocked the apoptosis-inducing effect of dsRNA, indicating the involvement of IFN-β autocrine signalling. Furthermore, studies on the intracellular signal transduction pathways revealed that dsRNA induces the activation of p38, ERK1/2 and JNK1/2 in melanocytes. Using specific inhibitors, we demonstrated that activation of p38 and ERK1/2 controlled both IFN-β secretion and IFN-β mediated cell death. Taken together, these data suggest that viral dsRNA stimulates TLR3 in human melanocytes and triggers the cellular apoptosis through autocrine of IFN-β.     

白念珠菌对角质形成细胞Toll样受体2表达的影响

目的:探讨白念珠菌和甘露聚糖对人角质形成细胞Toll样受体2(TLR2)表达的影响。方法:用白念活菌和甘露聚糖分别刺激培养的人角质形成细胞24h,然后用逆转录聚合酶链反应(RTPCR)检测细胞TLR2mRNA表达,免疫组化SP法检测TLR2蛋白表达,并进行半定量分析。结果:正常培养的人角质形成细胞有TLR2mRNA和蛋白表达,白念珠菌和甘露聚糖刺激角质形成细胞24h后,TLR2表达量没有统计学差异(P>0.05)。结论:角质形成细胞有TLR2mRNA和蛋白水平的组成性表达,白念珠菌和甘露聚糖对TLRR2表达均无明显影响,推测角质形成细胞表面可能存在多个识别念珠菌及其产物甘露聚糖的受体,TLR2只是其中之一。     

Oestrogen receptor beta is the predominant oestrogen receptor in human scalp skin

Oestrogens play a major role in non-classic target tissues in both sexes, yet there have been few studies on estrogens and skin. Recently a second oestrogen receptor (ERbeta) has been discovered. Therefore, we have compared the expression of oestrogen receptor alpha (ERalpha), beta (ERbeta), the androgen receptor (AR) and a cell proliferation marker in male and female non-balding scalp skin. ERbeta was the major steroid receptor expressed in human skin. It was highly expressed in epidermis, blood vessels and dermal fibroblasts, in contrast to ERalpha and AR. In the hair follicle, ERbeta expression was localized to nuclei of outer root sheath, epithelial matrix and dermal papilla cells, in contrast to ERalpha, and the AR, which was only expressed in dermal papilla cells. Serial sections also showed strong nuclear expression of ERbeta in the cells of the bulge, while neither ERalpha nor AR was expressed. In the sebaceous gland, ERbeta was expressed in both basal and partially differentiated sebocytes. ERalpha exhibited a similar pattern of expression, while the AR was expressed in the basal and very early differentiated sebocytes. There was no obvious difference in the expression of either oestrogen receptor in male or female skin. The wide distribution of ERbeta in human skin suggests that oestrogens may play an important role in the maintenance of skin and in the regulation of the pilosebaceous unit, and provides further evidence for oestrogen action in non-classic target tissues. The differential expression of ERalpha, ERbeta and AR in human skin suggests that the mechanisms by which steroid hormones mediate their effects may be more complex than previously thought.     

Cutaneous epithelioid angiomatous nodule of the chest wall with expression of estrogen receptor: a mimic of carcinoma and a potential diagnostic pitfall

Cutaneous epithelioid angiomatous nodule (CEAN) is a rare vascular proliferation that develops on the trunk and extremities. The lesion arises over weeks to months and affects both sexes without age predilection. Histologically, CEAN is characterized by a circumscribed proliferation of epithelioid endothelial cells in the superficial dermis with a background of lymphocytes, plasma cells and eosinophils. The epithelioid cells are positive for CD31, CD34 and/or D2-40. We report a case of CEAN that had remained stable for more than 30 years on the chest wall of a woman with a history of breast cancer. The lesional cells were epithelioid in appearance and positive for estrogen receptor (ER), raising suspicion for breast carcinoma. However, the cells were positive for CD31, CD34, D2-40 and EMA (epithelial membrane antigen); they were negative for cytokeratins, carcinoembryonic antigen (CEA), CD1a, gross cystic disease fluid protein (GCDFP-15), S-100, a melanocytic cocktail, HHV-8 and progesterone receptor. The histologic and immunohistochemical features, including a low proliferation index (10% by Ki-67), helped to distinguish this lesion from carcinoma and other vascular lesions. This is the most comprehensive immunohistochemical profile reported for CEAN to date and the first time that ER expression has been described.     

Repeated administration of IL‐31 upregulates IL‐31 receptor A (IL‐31RA) in dorsal root ganglia and causes severe itch‐associated scratching behaviour in mice

We investigated the effects of repeated administration of interleukin‐31 (IL‐31) on itch‐associated scratching counts (long‐lasting scratching, LLS) and IL‐31‐related receptor mRNA expression in mice. Intra‐dermal (i.d.) injection of IL‐31 (100 and 300 ng/site) every 12 h for 3 days significantly increased LLS. Repeated administration of IL‐31 also increased the expression of IL‐31 receptor A (IL‐31RA) and oncostatin M receptor beta (OSMRβ) in dorsal root ganglia (DRG). After the repeated administration of IL‐31 was discontinued, IL‐31RA expression decreased and reached the baseline level 2 days after the last dose of IL‐31. LLS changed along with DRG IL‐31RA expression. Moreover, IL‐31‐induced IL‐31RA protein expression was confirmed by Western blotting analysis. These data suggest that IL‐31 upregulates IL‐31RA expression in DRG neuron cell bodies, and cutaneous‐injected IL‐31‐induced itching is enhanced by DRG IL‐31RA expression in mice.     

CXC chemokine receptor 4 is essential for Lipo-PGE1-enhanced migration of human dermal fibroblasts

Abstract: Lipo‐PGE1 [EGLANDIN^®; a lipid microsphere‐incorporated prostaglandin E1 (PGE1)] stimulates angiogenesis and promotes the healing of skin ulcers. Because the effects of Lipo‐PGE1 on cutaneous wound healing are not completely understood, we investigated the ability of Lipo‐PGE1 to affect in vivo wound healing and regulate the migration of human dermal fibroblasts (HDFs). In a murine wound model, Lipo‐PGE1 reduced the wound size compared with control mice. Lipo‐PGE1 significantly increased HDF migration in a dose‐ and time‐dependent manner. Lipo‐PGE1 markedly increased the expression of CXC chemokine receptor 4 (CXCR4), which controls the migration of HDFs, at the mRNA and protein levels. Small interfering RNA (siRNA)‐mediated knockdown of CXCR4 inhibited Lipo‐PGE1–enhanced HDF migration. Moreover, Lipo‐PGE1 directly induced the phosphorylation of c‐Jun N‐terminal kinase (JNK), and the JNK‐specific inhibitor Sp6000125 blocked Lipo‐PGE1–enhanced migration and CXCR4 expression of HDFs. Our results demonstrate that Lipo‐PGE1 accelerates wound healing in vivo and increases the CXCR4‐mediated migration of HDFs through the JNK pathway.