共查询到20条相似文献,搜索用时 15 毫秒
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Mohamed M. Abd-Eldaim Rebecca P. Wilkes Kathy V. Thomas Melissa A. Kennedy 《Archives of virology》2009,154(4):555-560
Feline calicivirus (FCV) is a common cause of upper respiratory tract disease in cats and is associated with interstitial
pneumonia, oral ulceration and polyarthritis. Recently, outbreaks have involved a highly virulent FCV that leads to multisystemic
signs. Virus isolation and conventional RT-PCR are the most common methods used for FCV diagnosis. However, real-time RT-PCR
offers a rapid, sensitive, specific and easy tool for nucleic acid detection. The objective of this study was to design a
TaqMan probe-based, real-time RT-PCR assay for detection of FCV. It was determined in our previous study that the first 120
nucleotides of the 5′ region of the genome are highly conserved among FCV isolates. Primers and a probe specific for this
region were designed for a real-time RT-PCR assay to detect FCV. Initial validation was done using 15 genetically diverse
isolates. Also, 122 samples were tested by the new assay and virus isolation. The real-time RT-PCR assay was as sensitive
and specific as virus isolation and was far more rapid. This real-time RT-PCR assay targeting the conserved 5′ region of the
genome is a fast, economical and accurate method for detection of FCV. 相似文献
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Comparison of a lateral-flow immunochromatography assay with real-time reverse transcription-PCR for detection of human metapneumovirus 下载免费PDF全文
Kikuta H Sakata C Gamo R Ishizaka A Koga Y Konno M Ogasawara Y Sawada H Taguchi Y Takahashi Y Yasuda K Ishiguro N Hayashi A Ishiko H Kobayashi K 《Journal of clinical microbiology》2008,46(3):928-932
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Evaluation of reverse transcription-PCR and a bacteriophage-based assay for rapid phenotypic detection of rifampin resistance in clinical isolates of Mycobacterium tuberculosis. 总被引:17,自引:0,他引:17 下载免费PDF全文
I. J. Eltringham F. A. Drobniewski J. A. Mangan P. D. Butcher S. M. Wilson 《Journal of clinical microbiology》1999,37(11):3524-3527
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New reverse transcription-PCR assay for rapid and sensitive detection of enterovirus genomes in cerebrospinal fluid specimens of patients with aseptic meningitis 下载免费PDF全文
Jacques J Carquin J Brodard V Moret H Lebrun D Bouscambert M Motte J Rémy G Andréoletti L 《Journal of clinical microbiology》2003,41(12):5726-5728
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GeneScan reverse transcription-PCR assay for detection of six common respiratory viruses in young children hospitalized with acute respiratory illness 下载免费PDF全文
Erdman DD Weinberg GA Edwards KM Walker FJ Anderson BC Winter J González M Anderson LJ 《Journal of clinical microbiology》2003,41(9):4298-4303
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Comparison of an immunochromatography test with multiplex reverse transcription-PCR for rapid diagnosis of respiratory syncytial virus infections 下载免费PDF全文
Kuroiwa Y Nagai K Okita L Ukae S Mori T Hotsubo T Tsutsumi H 《Journal of clinical microbiology》2004,42(10):4812-4814
A new commercial rapid 10-min one-step immunochromatography (IC) test, SAS RSV test, was compared to another IC test, Directigen EZ RSV, employing RT-PCR as the "gold standard" for detecting respiratory syncytial virus. Of 102 clinical samples, 79 were positive by RT-PCR, 66 (82.5%) were positive with the SAS RSV test, and 55 (69.6%) were positive with Directigen EZ RSV. The specificity of the new test was 91.3% (21 of 23), similar to that of Directigen EZ RSV (100% [23 of 23]). This test performs well enough to be used for patient care. 相似文献
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Rapid detection of enterovirus RNA in cerebrospinal fluid specimens with a novel single-tube real-time reverse transcription-PCR assay. 总被引:7,自引:0,他引:7 下载免费PDF全文
Walter A. Verstrepen Sofie Kuhn Mark M. Kockx Martine E. Van De Vyvere An H. Mertens 《Journal of clinical microbiology》2001,39(11):4093-4096
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