HPLC法同时测定金黄连口服液中绿原酸和黄芩苷的含量

[目的]建立HPLC法同时测定金黄连口服液中绿原酸和黄芩苷的含量测定方法。[方法]色谱柱为Agilent TC-C18(4.6 mm×250 mm,5μm),流动相为乙腈-0.1%磷酸溶液进行梯度洗脱,流速为1.0 ml/min,检测波长为318 nm,柱温为35℃。[结果]绿原酸和黄芩苷的线性范围分别为0.1602~1.6016μg(r=0.999)和0.1002~1.0020μg(r=1.000)。绿原酸平均回收率为98.01%,RSD为1.21%;黄芩苷平均回收率为99.54%,RSD为1.16%。[结论]HPLC法简便、快捷、重复性好,可作为该制剂质量控制的方法。     

HPLC同时测定银黄颗粒中7种有机酸及4种黄酮类成分

目的 建立同时测定银黄颗粒中7种有机酸类成分（新绿原酸、绿原酸、隐绿原酸、咖啡酸和异绿原酸A、B、C）和4种黄酮类成分（黄芩苷、汉黄芩苷、黄芩素及汉黄芩素）的HPLC方法。方法 色谱柱为AkzoNobel Kromasil C₁₈柱（250 mm×4.6 mm,5 μm）;流动相为乙腈-0.1%磷酸水溶液,梯度洗脱;分段变波长测定;柱温30 ℃;体积流量1.0 mL/min。结果 11种成分的线性关系均良好,精密度、稳定性、重复性的RSD均低于2%,加样回收率为97.35%～99.23%。结论 该方法简便、准确、灵敏,可用于银黄颗粒质量标准提高研究。     

巴曲酶注射液对改进光化学法致栓塞性脑梗死家兔模型的改善研究

目的 建立同时测定银黄颗粒中7种有机酸类成分（新绿原酸、绿原酸、隐绿原酸、咖啡酸和异绿原酸A、B、C）和4种黄酮类成分（黄芩苷、汉黄芩苷、黄芩素及汉黄芩素）的HPLC方法。方法 色谱柱为AkzoNobel Kromasil C₁₈柱（250 mm×4.6 mm,5 μm）;流动相为乙腈-0.1%磷酸水溶液,梯度洗脱;分段变波长测定;柱温30 ℃;体积流量1.0 mL/min。结果 11种成分的线性关系均良好,精密度、稳定性、重复性的RSD均低于2%,加样回收率为97.35%～99.23%。结论 该方法简便、准确、灵敏,可用于银黄颗粒质量标准提高研究。     

HPLC法测定苦参汤有效部位中4种黄酮类成分含量

目的建立苦参汤有效部位中4种黄酮类成分黄芩苷、汉黄芩苷、黄芩素和汉黄芩素的HPLC含量测定方法.方法采用Phenomenex Luna C18柱(4.6 mm×250 mm,5 μm),甲醇-磷酸水溶液[磷酸-水(2:530)](45:55)为流动相,流速1 mL/min,检测波长280 nm.结果黄芩苷平均回收率为99.28%,RSD=1.92%(n=5),汉黄芩苷平均回收率为98.48%,RSD=1.53%(n=5),黄芩素平均回收率为99.51%,RSD=1.81%(n=5),汉黄芩素平均回收率为97.43%,RSD=2.14%(n=5).结论3批样品测定结果表明,该法简便、准确,可用于苦参汤有效部位中黄芩苷等4种黄酮类成分含量测定.     

基于超高效液相-四极杆飞行质谱联用技术的牛黄清感胶囊有效成分含量测定方法研究

目的:建立同时测定牛黄清感胶囊中7种成分的含量的方法,进而用于该制剂的质量控制。方法:采用超高效液相色谱-四极杆飞行时间质谱(UPLC-Q-TOF/MS)联用技术。Waters Acquity UPLC BEH C18色谱柱(100mm×2.1mm,1.7μm),AQUITY UPLC BEH C18 VanGuard Pre-Column预柱(100mm×2.1mm,1.7μm),流动相为0.1%甲酸水(A)-0.1%甲酸乙腈(B),梯度洗脱,流速为0.3mL/min,柱温为30℃,进样量为5μL。质谱采用电喷雾(ESI)离子源,离子化模式为电喷雾正离子模式。一级质谱母离子扫描范围为80~1500数据采集软件为Analyst TF1.6 software;数据处理软件系统为Peakview 2.0/masterview 1.0software、Markerview 1.2.1。结果:3批次牛黄清感胶囊中7个成分,白杨素、异亮氨酸、当药苷、金丝桃苷、牛黄胆酸钠、咖啡酸、苯丙氨酸进样量检测线性范围分别为0.0206~0.309、0.0106~0.159、0.021~0.315、0.021~0.315、1.03~15.45、0.041~0.615、0.0105~0.1575mg(r≥0.9994),含量分别为0.158、0.0418、0.2345、0.2489、9.6476、0.0189、0.0139mg/g。精密度、稳定性(24h)、重复性试验的RSD均小于2.0%(n=6),平均加样回收率为99.2%~100.7%,RSD<2.0%(n=6)。结论:该方法稳定、准确、重现性好,专属性强,可用于牛黄清感胶囊的质量控制。     

同时测定黄芩提取液中黄芩苷、汉黄芩苷、黄芩素含量的高效液相色谱方法的建立

目的 建立同时测定黄芩苷、黄芩素、汉黄芩苷含量的高效液相色谱(HPLC)方法,并对不同黄芩提取液中三种成分的含量进行测定。方法 采用不同浓度的乙醇及蒸馏水提取黄芩,减压浓缩制备黄芩提取液,通过专属性考察、线性关系测定、精密度测定、稳定性考察等建立黄芩苷、黄芩素、汉黄芩苷同时测定的HPLC-紫外检测法,并采用该方法测定不同提取液中三种成分含量。结果 同时测定黄芩苷、黄芩素、汉黄芩苷的HPLC条件为:色谱柱为Agilent Eclipse XDB-C₁₈(250 mm×4.6 mm,5 μm),流动相为乙腈-0.05%磷酸水溶液(25∶75);流速1.00 mL/min;紫外检测波长275 nm;进样量10 μL;柱温30℃。不同溶剂对黄芩中黄芩苷、黄芩素、汉黄芩苷的溶出有很大影响。黄芩苷、黄芩素、汉黄芩苷质量浓度在0.97～19.45,1.71～34.10,0.61～12.25 μg/mL内线性关系良好,仪器的精密度,方法的重复性、稳定性良好,RSD均在2.0%以内。结论 HPLC-紫外检测法灵敏度高,精密度好,专属性强,可用于黄芩不同提取液中黄芩苷、黄芩素、汉黄芩苷含量的测定。     

高效液相色谱法测定消痔胶囊中黄芩苷的含量

[目的]建立消痔胶囊中黄芩苷含量测定的方法。[方法]采用高效液相色谱法,Zorbax C18柱(4.6mm×250mm,5μm),流动相为甲醇-水-磷酸(52∶48∶0.2),检测波长为280nm,流速为1.0ml/min。[结果]黄芩苷在0.100 2~1.603 2μg范围内呈良好的线性关系,r=0.999 8,平均回收率为99.3%,RSD=0.98%(n=5)。[结论]HPLC法简便、快速、准确,可作为消痔胶囊的质量控制方法。     

高效液相色谱法测定清喉利咽颗粒中黄芩苷、黄芩素和汉黄芩素的含量

目的 建立清喉利咽颗粒中黄芩苷、黄芩素和汉黄芩素含量的测定方法.方法 采用高效液相色谱法(high performance liquid chromatography,HPLC).色谱柱为DiamonsilTM C18(150 mm×4.6 mm,5 μm),乙腈-0.6%磷酸水溶液(40:60)为流动相,柱温,35℃,流速,1.0 mL/min,检测波长,277 nm.结果 方法精密度及线性关系良好,黄芩苷、黄芩素和汉黄苓素平均回收率分别为100.5%[相对标准差(relative standard deviation,RSD)=0.70%]、100.4%(RSD=1.2 %)和100.5%(RSD=0.83%)(n=9).结论 该法操作简便,结果准确,灵敏度高.     

高效液相色谱法测定牛黄化毒片中连翘苷含量*

[目的]建立以高效液相色谱法测定牛黄化毒片中的连翘苷含量的方法。[方法]色谱为C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙睛-水(23∶77),流速为1.0 mL/min,检测波长为230 nm,柱温为室温。[结果]连翘苷检测浓度在0.194 6～2.919 0μg范围内与峰面积值线性关系良好(r=0.999 9),平均回收率为99.90%[相对标准偏差(RSD)=1.69%]。[结论]该方法可用于牛黄化毒片的质量检测。     

多波长高效液相法同时测定鼻渊舒口服液中6种成分的含量

目的:建立同时测定鼻渊舒口服液中绿原酸、栀子苷、阿魏酸、黄芩苷、黄芩素、汉黄芩素含量的方法。方法:采用多波长高效液相色谱法测定绿原酸、栀子苷、阿魏酸、黄芩苷、黄芩素和汉黄芩素的含量,色谱柱为Phenomenex Gemini C_(18)色谱柱(4.6 mm×250.0 mm×5μm),甲醇(A)-(体积分数)0.2%磷酸水溶液(B)为流动相,梯度洗脱(0～7 min,10%A;7～12 min,10%～20%A;12～13 min,20%～30%A;13～26 min,30%～65%A;26～35 min,60%～70%A;35～45 min,10%A),流速为1.0 mL·min~(-1),柱温为40℃,检测波长(0～18.5 min,327nm;18.5～20 min,238n m;20～23 min,321nm;23～45 min,280 nm)。结果:绿原酸、栀子苷、阿魏酸、黄芩苷、黄芩素、汉黄芩素分别在35.2～352.0μg、8.4～84.0μg、1.28～12.8μg、6.0～60.0μg、1.6～16.0μg、1.68～16.8μg范围内线性关系良好;平均回收率依次为绿原酸100.47%(RSD=0.52%),栀子苷101.61%(RSD=1.69%),阿魏酸101.11%(RSD=1.32%),黄芩苷101.26%(RSD=1.24%),黄芩素101.09%(RSD=0.95%),汉黄芩素100.83%(RSD=1.76%)。结论:此方法具有操作快速、简便、重复性好的特点,可用于提高鼻渊舒口服液的质量标准。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.