许旺细胞在不同孔径丝素蛋白支架上的生长

背景：细胞在生物支架上的生长行为受到支架表面形貌、润湿性、孔径及孔隙率等多种因素影响。目的：观察许旺细胞在不同孔径丝素蛋白支架上的生长情况。方法：制备大孔径50～60μm、小孔径10～20μm两种多孔丝素材料。选用许旺细胞永生化细胞R3[33-10ras3]为种子细胞,当细胞在培养瓶底形成致密单层时即可消化细胞并进行接种实验,将许旺细胞悬液种于不同形貌的多孔丝素材料表面。复合培养1周后,扫描电镜观察许旺细胞的生长形态及增殖等情况。结果与结论：不同孔径丝素材料的表面可见许旺细胞生长情况不一。在10～20μm孔径材料支架上,细胞浓度较低,细胞表现为特异的双极性形态,细胞与细胞之间或平行排列,或首尾相连成细胞链;细胞与细胞之间或平行排列,或首尾相连成细胞链;在50～60μm孔径丝素材料支架上,细胞浓度较高,细胞多为球形,单个分散在多孔支架表面,或呈现成团成串葡萄样聚集在孔的底部,未延展成双极性形态,只有极少量生长在孔与孔之间嵴上的细胞呈双极样。说明多孔丝素蛋白支架的孔径对许旺细胞的黏附、生长有一定的影响,许旺细胞更适合生长在孔径略大于胞体直径的支架材料上。     

热致相分离聚合物多孔膜实验分析

目的:分析热致相分离成膜过程的机理、热力学理论基础、动力学机制以及影响因素,以便充分掌握影响孔度大小、分布、形态的因素,使孔度调控范围得以拓宽,使多孔膜的制备能重复可控。方法:从组织工程材料结构特点出发,分析热致相分离聚合物多孔膜的制备方法及该法成膜的热力学理论基础、动力学机制以及影响因素,并分析实验中存在的问题及今后的研究方向。结果:①以热致相分离法可制备聚合物多孔膜。②热致相分离法制备多孔膜是高聚物均相溶液在淬冷条件下发生相分离的过程,它适用于上临界共溶温度型聚合物-稀释剂二元体系。③热致相分离法成膜的过程,可以认为是旋节线机理占主导地位。④热致相分离法制备的微孔材料,其孔隙率、孔径大小、结构形态与聚合物稀释剂的种类、组成配比、聚合物浓度、聚合物分子量等因素密切相关。结论:①可采用热致相分离技术制备多孔膜,通过改变不同的成膜条件可获得一系列不同孔径尺寸和孔径分布的多孔膜材料。②对热致相分离成膜过程中聚合物-溶剂体系的相图测定,不同厚度的多孔膜形貌研究,不同χ(聚合物-溶剂相互作用参数)体系所制备的多孔膜形貌等需深入研究。     

Gro许旺细胞在不同孔径丝素蛋白支架上的生长(英文)

背景:细胞在生物支架上的生长行为受到支架表面形貌、润湿性、孔径及孔隙率等多种因素影响。目的:观察许旺细胞在不同孔径丝素蛋白支架上的生长情况。方法:制备大孔径50～60μm、小孔径10～20μm两种多孔丝素材料。选用许旺细胞永生化细胞R3[33-10ras3]为种子细胞,当细胞在培养瓶底形成致密单层时即可消化细胞并进行接种实验,将许旺细胞悬液种于不同形貌的多孔丝素材料表面。复合培养1周后,扫描电镜观察许旺细胞的生长形态及增殖等情况。结果与结论:不同孔径丝素材料的表面可见许旺细胞生长情况不一。在10～20μm孔径材料支架上,细胞浓度较低,细胞表现为特异的双极性形态,细胞与细胞之间或平行排列,或首尾相连成细胞链;细胞与细胞之间或平行排列,或首尾相连成细胞链;在50～60μm孔径丝素材料支架上,细胞浓度较高,细胞多为球形,单个分散在多孔支架表面,或呈现成团成串葡萄样聚集在孔的底部,未延展成双极性形态,只有极少量生长在孔与孔之间嵴上的细胞呈双极样。说明多孔丝素蛋白支架的孔径对许旺细胞的黏附、生长有一定的影响,许旺细胞更适合生长在孔径略大于胞体直径的支架材料上。     

适宜于构建组织工程化脂肪的蚕丝蛋白支架:最佳孔径筛选

背景:既往组织工程脂肪的研究中,支架材料的孔径往往被忽略.如孔径过大,细胞会顺着过大的孔隙流走,难以保留在支架中;孔径过小,细胞则主要分布于支架材料的表面,不易进入支架中,同时也不利于新生血管生长.目的:筛选用于构建组织工程脂肪的蚕丝蛋白支架的适宜孔径.方法:在保持蚕丝蛋白浓度不变条件下,通过改变冷冻干燥温度与时间,制备出6批次不同孔径的蚕丝蛋白支架;贴壁法分离脐带间充质干细胞,化学染色检测其体外诱导成骨和成脂的能力;电镜下测定6批次蚕丝蛋白支架的孔径;观察脐带间充质干细胞在不同孔径蚕丝蛋白支架上黏附、增殖的能力.结果与结论:6批次蚕丝蛋白支架的孔径分别为:(39.94±17.27),(53.51±16.18),(63.97±19.76),(71.08±18.07),(87.33+21.78),(121.97+44.10)μm.脐带间充质干细胞在2号支架材料上黏附良好,并充分伸展,而第1,3,4,5,6号支架材料,除第1,3号支架上偶见细胞外,其余支架材料上均未见细胞生长.由此说明人脐带间充质干细胞与蚕丝蛋白支架构建组织工程脂肪时,支架材料的最佳孔径为50 μm.     

不同孔径纳米羟基磷灰石人工骨修复兔桡骨缺损效果比较

目的:纳米级的羟基磷灰石材料与人体内组织成分更为相似,具有更佳的生物性能。评价不同孔径的多孔纳米羟基磷灰石人工骨的骨缺损修复能力,从而筛选出适合的孔径以达到骨传导功能与生物力学性能的良好统一。方法:实验于2005-10/2006-10在深圳市第二人民医院中心实验室完成。①实验材料:纳米羟基磷灰石人工骨以硝酸钙和磷酸二氢铵为原料,采用溶胶-絮凝法制备粉体,运用压力成型、木模成型和浸渍成型分别制得孔隙分布均匀的孔径分别为50～150μm、100～250μm和300～500μm的多孔纳米羟基磷灰石人工骨。②实验动物:雄性新西兰大白兔60只随机分为植入50～150μm孔径材料组、植入100～250μm孔径材料组、植入300～500μm孔径材料组、空白对照组,每组15只。实验过程中对动物处置符合动物伦理学要求。③实验方法:制备双侧桡骨骨缺损动物模型,然后用3种不同孔径的纳米羟基磷灰石人工骨材料植入骨缺损处进行修复,空白对照组不植入任何材料。④实验评估:术后4,8和12周分别行大体标本观察、X射线片观察、扫描电镜观察及生物力学测试,比较各组材料修复骨缺损的能力。结果:实验动物均进入结果分析。①X射线片检查结果:术后4周、8周、12周,植入100～250μm孔径材料组X射线评分高于植入50～150μm,300～500μm孔径材料组,差异有显著性意义(P<0.05)。②生物力学检测结果:术后4周、8周、12周,植入100～250μm孔径材料组生物力学强度高于植入50～150μm,300～500μm孔径材料组,差异有显著性意义(P<0.05)。③扫描电镜观察结果:植入100～250μm孔径材料组成骨效果明显优于植入50～150μm,300～500μm孔径材料组和空白对照组。结论:纳米羟基磷灰石人工骨具有良好的成骨能力,但其骨修复能力受孔径因素的影响,孔径100～250μm的纳米羟基磷灰石人工骨材料成骨能力较好。     

聚醋酸乙烯酯多孔材料的制备工艺及其表征

背景:泡孔的直径及其分布的控制是制备多孔聚合物材料的难题,但目前尚无有效的解决办法.目的:采用高内相比乳液模板法,以Span80为引发剂,制备聚醋酸乙烯酯多孔材料,分析制备工艺对其泡扎直径及其分布的影响.设计,时间及地点:观察实验,于2008-12/10在泰山医学院高分子化学实验室完成.材料:醋酸乙烯酯由天津永大化学工业公司提供;二乙烯基苯、Span80由美国Aldrich公司提供;无水氯化钙、氯苯由上海第一试剂公司提供;甲苯由天津纵横兴化学工业公司提供;1,2-二氯苯由北京精益化学试剂公司提供.方法:向装有电动搅拌器和温度计的250 mL三颈烧瓶中加入连续相(醋酸乙烯酯、二乙烯基苯、致孔剂等)和乳化剂,搅拌并通过蠕动泵缓慢滴加含有过硫酸钾和CaCl2的水溶液,并控制反应温度.滴加完毕后,继续搅拌,移至60℃的水浴中继续聚合.然后将聚合好的坚硬的固体,切成1 cm厚的小圆盘状,装入Soxlet提取器中,用无水乙醇作溶剂抽提48 h.再将抽提好的崮体放入烘箱中,在50℃F干燥即可制得聚醋酸乙烯酯多孔材料.扫描电镜观察泡孔直径、窗口直径,3H-2000Ⅱ型全自动氮吸附比表面积测试仪测定多孔材料的比表面积.主要观察指标:多孔材料的比表面积、泡孔直径、窗口直径.结果:制备的聚醋酸乙烯酯多孔材料的平均泡孔直径3.3～5.2 μ m,窗口直径1.7～2.3 μ m.在反应体系中加入某些非反应性溶剂如甲苯等替代部分反应单体可制得比表面积高达720 m2/g的聚醋酸乙烯酯多孔材料.结论:采用高内相比模板法,通过控制乳液前躯体的反应条件和反应体系的组成,可以制备出泡孔直径可控的聚醋酸乙烯酯多孔材料.     

人牙髓细胞复合不同孔径羟基磷灰石/磷酸三钙支架材料的生物学行为

背景:有文献表明,通过组织工程的方法将人牙髓细胞复合羟基磷灰石/磷酸三钙多孔支架材料修复牙缺损具有可行性.然而究竟多大孔径的支架材料最有利于人牙髓细胞的生长及分化,至今尚无定论.目的:观察人牙髓细胞复合不同孔径羟基磷灰石/磷酸三钙支架材料后黏附、增殖和分化等生物学行为.方法:人牙髓细胞接种至3种不同孔径的羟基磷灰石/磷酸三钙材料上,采用荧光显微镜以及扫描电镜检测细胞在材料表面的黏附生长情况,然后通过细胞的黏附率实验与MTT比色法观察人牙髓细胞在材料表面的黏附与增殖特性.不同孔径的羟基磷灰石/磷酸三钙支架复合人牙髓细胞后分别用生长培养基和矿化诱导液培养,于接种后第4,7,10天检测碱性磷酸酶活性.结果与结论:人牙髓细胞在3种不同孔径支架材料表面和孔隙内均能顺利黏附并增殖.其中100～300 μm组支架材料黏附率最高,MTT结果显示接种3 d后300～500 μm组能较好地促进细胞增殖.培养10 d后,复合在100～300 μm和300～500μm材料上的人牙髓细胞,其碱性磷酸酶活性显著高于500-700 μm组.提示与500～700 μm孔径相比,孔径为100～300μm和300～500 μm的羟基磷灰石/磷酸三钙材料能更好地促进人牙髓细胞黏附、增殖和分化.     

制备软骨组织工程支架的方法

背景：软骨组织工程支架作为软骨细胞外基质的替代物,其外形和孔结构对实现其作用和功能具有非常重要的意义。 目的：回顾目前若干种常用软骨组织工程中三维多孔支架的制备方法。 方法：由第一作者检索2000至2013年PubMed数据库,ELSEVIER SCIENCEDIRECT、万方数据库、中国知网数据库。英文检索词为＂Cartilage tissue engineering;scaffolds;fabrication＂,中文检索词为＂软骨组织工程;制备方法;支架材料;多孔支架＂。 结果与结论：制备软骨组织工程支架的方法有相分离/冷冻干燥法、水凝胶技术、快速成型技术、静电纺丝法、溶剂浇铸/粒子沥滤法及气体发泡法等。目前研究发现,支架中孔径的大小对组织的重建有着直接的影响,孔径为100-250 μm的孔有益于骨及软骨组织的再生。通过溶液浇铸/粒子沥滤法、气体发泡法所制备的支架孔径大小在这一范围内,因此比较适合用于骨、软骨组织工程支架的构建。研究人员通常将多种方法结合起来,以期能制备出生物和力学性能方面更加仿生的组织工程多孔支架。     

组织工程脊髓支架材料:聚乳酸-羟基乙酸最佳孔径的筛选

背景:细胞支架是细胞生长的载体,其孔径是影响组织工程脊髓疗效的重要因素之一.目的:通过将神经干细胞与不同孔径的聚乳酸-羟基乙酸(poly lactic-co-glycolic acid,PLGA)支架体外复合培养,筛选确立组织工程脊髓支架材料的最佳孔径.方法:取传第1代的神经干细胞悬液50 pL(细胞数10~(10)L~(-1)),分别种植在孔径200～300 μm、400-500 μm的PLGA支架上复合培养7 d,得到两种组织工程脊髓.30只大鼠均建立脊髓损伤模型,造模后分为3组,分别在脊髓缺损处立即填塞上述两种组织工程脊髓,空白对照组在缺损处不进行材料移植.倒置相差显微镜及电镜下观察神经干细胞在PLGA支架中的生长增殖与分布,MTT检测两种组织工程脊髓所含神经干细胞的相对数量,采用BBB运动功能评分比较不同孔径的组织工程脊髓的移植疗效.结果与结论:镜下神经干细胞在各孔径材料上均紧密贴附并增殖分化,组织相容性良好.共培养7 d后,孔径200～300 μmPLGA支架组、孔径400-500 μm PLGA支架组的吸光度值基本相似(P>0.05),说明PLGA支架的孔径大小对培养的神经干细胞增殖数量无明显影响.移植第4周与空白对照组比较,孔径200-300 μm PLGA支架组、孔径400～500 μm PLGA支架组大鼠的神经功能均有不同程度恢复,BBB运动功能评分均明显升高(P<0.05),且孔径200-300 μm的PLGA支架其移植效果更好.     

不同孔径的国产PDLLA填充材料修复骨缺损实验研究

目的评价不同孔径三维立体泡沫状外消旋聚乳酸材料（PDLLA）的成骨能力。方法采用溶剂注模并盐结晶颗粒沥滤法制成3种（70～150μm,150～300μm和400～700μm）孔径的外PULLA料,在62例1cm兔桡骨去骨膜缺损模型中分别植入3种孔径材料和空白对照,术后2、4、8、12周行X线、组织学观察及8、12周扫描电镜观察骨缺损区骨生成情况。结果70～150μm与150～300μm材料成骨较好,与400～700μm组和对照组相比差异有显著性P（<0.05）。结论PDLLA具有良好的生物相容性和可吸收性,制成泡沫状具有较好的骨传导性能。     

Effect of fractal dimension on drug permeation through porous ethylcellulose films

Fractal geometry was applied to quantify the complexity of an internal structure of a porous film prepared with ethylcellulose (EC) and diethylphthalate (DEP) as a plasticizer. EC was dissolved together with DEP in a water–ethanol mixture solution, and then evaporated on Teflon petri dishes in order to make porous EC films. Boundary lines of the porous structures in the EC film cross section were taken by a confocal laser microscope as image data, and these images were fed into a computer to estimate the fractal dimension. The porous structure in EC film was observed to be a typical fractal and its complexity was quantified as a non-integral fractal dimension. No clear correlation was observed between the fractal dimension and the porosity of EC films, suggesting that they were mutually independent parameters representing the porous structure in the EC films. The permeation of theophylline through the EC films was determined by using two-chamber diffusion cells. A fairly good relationship between the permeability coefficient of theophylline and the fractal dimension was observed, suggesting the usefulness of the fractal dimension as a novel parameter for evaluating drug permeation through porous films.     

Effects of lipid composition on membrane distribution and permeability of natural quinones

Natural quinones are amphiphilic molecules that function as mobile charge carriers in biological energy transduction. Their distribution and permeation across membranes are important for binding to enzymatic complexes and for proton translocation. Here, we employ molecular dynamics simulations and free energy calculations with a carefully calibrated classical force-field to probe quinone distribution and permeation in a multi-component bilayer trying to mimic the composition of membranes involved in bioenergetic processes. Ubiquinone, ubiquinol, plastoquinone and menaquinone molecules with short and long isoprenoid tails are simulated. We find that penetration of water molecules bound to the polar quinone head increases considerably in the less ordered and porous bilayer formed by di-linoleoyl (18:2) phospholipids, resulting in a lower free energy barrier for quinone permeation and faster transversal diffusion. In equilibrium, quinone and quinol heads localize preferentially near lipid glycerol groups, but do not perform specific contacts with lipid polar heads. Quinone distribution is not altered significantly by the quinone head, tail and lipid composition in comparison to a single-component bilayer. This study highlights the role of lipid acyl chain unsaturation for permeation and transversal diffusion of polar molecules across biological membranes.

Lipid acyl chain unsaturation modulates the barrier for ubiquinone flip-flop over the membrane.     

Involvement of fractal geometry on solute permeation through porous poly (2-hydroxyethyl methacrylate) membranes.

Fractal geometry was applied to quantify the complexity of an internal structure of porous membranes prepared with poly(2-hydroxyethyl methacrylate) (pHEMA). The porous pHEMA membranes were synthesized by means of free-radical solution polymerization. Boundary lines of the porous structures in the pHEMA membrane were taken by a scanning electron microscope as image data, and these images were fed into a computer to estimate the fractal dimension. The boundary images of porous pHEMA membranes were observed to be a typical fractal and their complexity was quantified as a non-integral fractal dimension. The permeation of fluorescein isothiocyanate-labeled dextran, molecular weight 4400 (FD-4) as a model penetrant through the porous pHEMA membrane was determined using water-jacket type two-chamber diffusion cells. A fairly good negative relationship between the permeability coefficient of FD-4 and the fractal dimension was observed, suggesting the usefulness of the fractal dimension as a novel means for evaluating solute permeation through the porous membranes.     

Synthesis and characterization of divinyl‐fumarate poly‐ε‐caprolactone for scaffolds with controlled architectures

A vinyl‐terminated polycaprolactone has been developed for tissue engineering applications using a one‐step synthesis and functionalization method based on ring opening polymerization (ROP) of ε‐Caprolactone, with hydroxyl ethyl vinyl ether (HEVE) acting both as the initiator of ROP and as photo‐curable functional group. The proposed method employs a catalyst based on aluminium, instead of the most popular Tin(II) 2‐ethylhexanoate, to reduce the cytotoxicity. Following the synthesis of the vinyl‐terminated polycaprolactone, its reaction with fumaryl chloride (FuCl) results in a divinyl‐fumarate polycaprolactone (VPCLF). The polymers obtained were thoroughly characterized using Fourier transform infrared spectroscopy (FTIR) and gel permeation chromatography (GPC) techniques. The polymer has been successfully employed, in combination with N‐vinyl pyrrolidone (NVP), to fabricate films and computer‐designed porous scaffolds by micro‐stereolithography (μ‐SL) with gyroid and diamond architectures. Characterization of the networks indicated the influence of NVP content on the network properties. Human mesenchymal stem cells adhered and spread onto VPCLF/NVP networks showing good biological properties and no cytotoxic effect. Copyright © 2016 John Wiley & Sons, Ltd.     

Bi-static high resolution imaging for ship targets with sparse apertures and its application in InISAR

ABSTRACT

In this letter, we focus on the high-resolution imaging of ship targets under sparse aperture condition and its application in three-dimensional (3D) imaging. A bi-static high-resolution algorithm dealing with the sparse echo condition is proposed, which consists of the following processing steps: (1) A side-lobe apodization–multiple orthogonal least-square method is proposed to recover the complete signal and reduce the influence of side-lobe at the same time, which enhances the precision of phase extraction in interferometric inverse synthetic aperture radar (InISAR) imaging; (2) The bi-static configuration is used to accentuate the relative motion between the target and radar, such that the requirement for long coherence processing time can be avoided and (3) The 3D InISAR technique is employed to obtain the 3D images of the ship targets. Experiments with simulated and real data are utilized to demonstrate the effectiveness of the proposed method.     

相控阵超声断层成像诊断仪孔径聚焦声场的研究

孔径聚焦技术是B型超声诊断仪中一种提高近场区域横向分辨力的有效手段。本文根据相控阵换能器的远场指向性理论和近场声压分布理论,对孔径聚焦声场进行了分析和数值计算,在此基础上给出了相控阵超声断层成像诊断仪中相控阵换能器(64阵元)在孔径聚焦过程中孔径的变化范围及孔径聚焦所适应的区域深度。     

Determination of molecular weight distributions and mean molecular-weight values of clinical dextrans]

Clinical dextrans can be separated in fractions of different molecular size by permeation chromatography on porous glass columns. The apparatus, which is rather simple, is described in detail. The conversion of the elution diagrams in molecular weight distributions is possible if an adequate calibration curve and the magnitude of peak broadening are known. This information can be obtained experimentally. The analysis of the elution diagram is uncomplicated and generally applicable if both the elution curve and the boradening function are approximately Gaussian distributions. Usually this is the case in the systems in question. With the method described clinical dextrans can be characterized as to their molecular weights with high accuracy. The separation method can also be applied to the production of dextran fractions of narrow molecular weight distributions in a preparative scale.     

SMGA gels for the skin permeation of haloperidol.

Small molecule gelling agent (SMGA) gels were developed using the gelator GP-1 in the solvents, namely, isostearyl alcohol (ISA) and propylene glycol (PG), to deliver haloperidol through the skin. The concentrations of the drug, haloperidol, the enhancer, farnesol and the gelator, GP-1 are 3 mg/ml, 5% (w/v) and 5% (w/v), respectively. The study employed a three-factor full factorial statistical design to investigate the influence of factor level changes on the permeability coefficient and permeation lag-time of haloperidol. Gels were prepared by raising temperature to 120 degrees C, followed by natural cooling under room temperature of 22+/-1 degrees C. The rheological properties of the gels were examined with a strain-controlled dynamic mechanical method. The in vitro permeation study was conducted with automated flow-through type cells. The gels successfully incorporated the drug and enhancer without losing their aesthetic properties. The in vitro human skin permeation study showed the permeation of the drug in ISA-based gels reached the pseudo steady state faster than PG-based gels and were less affected by gelator. PG-based gels delivered the drug at a faster rate with the incorporation of the enhancer. GP-1 did not influence the drug permeation rate but it increased permeation lag-time. The co-existence of gelator or enhancer increased the lag-time to a larger extent than when used separately. The novel SMGA gels are suitable for topical or transdermal delivery.     

Phospholipids affect stratum corneum lipid bilayer fluidity and drug partitioning into the bilayers.

Phospholipids, e.g. fluid-state EPC (l-alpha-phosphatidylcholine from egg yolk), may diffuse into the stratum corneum and enhance dermal and transdermal drug penetration, while many other phospholipids, e.g. gel-state DSPC (distearoylphosphatidyl choline), are not able to do this. These effects are suggested to be due to the interactions between the phospholipids and the skin lipid bilayers, and so an in vitro method was developed to evaluate the influence of phospholipids on the distribution of drugs to stratum corneum lipids. The distribution coefficients of estradiol, progesterone and propranolol between stratum corneum lipid liposomes (SCLLs) without phospholipids or with EPC, DSPC, SPC (l-alpha-phosphatidylcholine from soybean) or DOPE (dioleylphosphatidyl ethanolamine), and pH 7.4 buffer were determined. Fluid-state phospholipids in SCLLs increased the partitioning of drugs into SCLLs, while gel-state lipid, DSPC, did not. The increased distribution of drugs into the SCLLs was at least partially due to the increased fluidity of SCLL bilayers by phospholipids, which was shown using steady-state fluorescence anisotropy. This in vitro method enables screening of the effects of phospholipids and other permeation enhancers on stratum corneum bilayer fluidity and drug partitioning.     

壳聚糖支架上骨髓间充质干细胞的浓度及黏附生长

背景：组织工程的研究重点是利用少量的细胞经体外培养、扩增后, 在一定环境下附着在三维多孔支架上并良好生长为后期的组织器官重建修复做好基础。目的：对不同浓度兔骨髓间充质干细胞复合至壳聚糖支架用于组织工程再生修复进行评价。方法：取 5×105脱乙酰度为 95%的壳聚糖粉末通过冷冻干燥法制备壳聚糖支架,取 1×106,1×107,1×108,1×109 L-1细胞体积各 100 μL复合至壳聚糖支架后 1,3,5,7,9 d 以光镜,扫描电镜,MTT 法观察骨髓间充质干细胞的生长与分裂增殖情况。结果与结论：壳聚糖海绵状多孔支架为 5 mm×5 mm×3 mm,孔径 190~380 μm,平均孔径 290 μm,孔相通性较好,空隙率为（84.00±4.62）%。细胞/支架共培养 72 h 后各浓度细胞组均可渗入壳聚糖支架多孔结构内黏附生长。1×107,1×108,1× 109 L-1浓度细胞组在支架上成蔟生长,部分细胞与支架融合。结果提示,1×107,1×108L-1组细胞更利于骨髓间充质干细胞在壳聚糖支架的黏附生长,用于组织再生修复。