外阴白色病变患者用壮阳祛痰法为主治疗结果及与相关因素的临床探讨

目的探讨壮阳祛痰法--消白饮治疗外阴白色病变的结果及对性激素、免疫球蛋白和微量元素的影响.方法对82例外阴白色病变患者经病理检查确诊后,分析病因为肾阳不足,痰湿阻络,阴器失养而致,采用自拟消白饮(由淫羊藿、仙茅、桂枝等药组成),同时分别于治疗前后检测自身血清性激素(FSH、LH、E2、P、T);免疫球蛋白(IgG、IgA、IgM);头发微量元素(Cu、Zn、Fe、Ca、Mg)水平.结果治疗后治愈69例,占85.4%,总有效率100%,治疗后检测FSH、LH、下降,E2、P、T、IgG、IgA、上升,与治疗前比较,有显著性差异(P＜0.05),IgM、微量元素比值与治疗前比较,差异无显著性(P＞0.05).结论壮阳祛痰法对外阴白色病变患者有较好的治疗效果,具有提高E2、P、T水平,增强机体免疫功能的作用.     

免疫球蛋白在丙型肝病中的结果分析

目的分析免疫球蛋白(IgA、IgG和IgM)在丙型肝病中的临床意义。方法随机抽取在邯郸市传染病医院住院的丙型肝炎患者200例和正常体检者30例,进行血清IgA、IgG和IgM检测,然后分析结果。结果①IgG水平随着丙型肝炎加重程度呈现升高趋势,IgA和IgM变化不明显。②IgA和IgG的正常范围所占比例随丙型肝炎病情加重呈现下降的趋势,而IgM变化不明显。结论 IgA、IgG水平随丙型肝病病情加重呈现升高的趋势,且IgG升高趋势优于IgA。     

血清免疫球蛋白在乙型肝炎患者临床检验的意义

目的:分析血清免疫球蛋白在乙型肝炎患者临床检验的意义。方法:检测对比观察组、健康体检者IgG、IgA、IgM水平,治疗后改善者、未改善者IgG、IgA、IgM水平。结果:观察组甲组IgG、IgA、IgM水平观察组乙组对照组,经治疗,改善者IgG、IgA、IgM小于未改善者,且P0.05。结论:血清免疫球蛋白可作为诊断乙型肝炎的重要指标。     

慢性阻塞性肺疾病急性加重期患者免疫球蛋白测定及其意义

目的　探讨慢性阻塞性肺疾病 (COPD)急性加重期患者血清免疫球蛋白的水平及其临床意义。方法　COPD急性加重期 4 4例和正常健康对照组 30例均取清晨空腹静脉血测定其血清免疫球蛋白 (IgM、IgA、IgG)的水平 ,并比较两组之间有无差异。 结果　COPD急性加重期患者血清免疫球蛋白的水平分别为 :IgM (1 70± 0 6 7) g/L、IgA (2 5 3± 1 2 7)g/L、IgG (11 86± 3 78) g/L ;正常健康对照组血清免疫球蛋白的水平分别为 :IgM (1 92± 0 76 )g/L、IgA (1 71± 0 6 8) g/L、IgG (11 12± 2 72 ) g/L。两组比较 ,IgM、IgG的水平差异无显著意义 (P >0 0 5 ) ,而IgA的水平差异有显著意义 (P <0 0 1)。 结论　COPD急性加重期患者血清IgA水平的升高可能与呼吸道感染有关。     

血清免疫球蛋白水平的变化对慢性乙型肝炎患者病情诊断的影响

目的:探究血清免疫球蛋白水平的变化对慢性乙型肝炎(CHB)患者病情诊断的影响。方法:选取医院2017年7月—2018年8月期间收治的CHB患者69例资料设为肝病组,根据肝病组患者肝功能损害程度的不同将其分为轻度组(25例)、中度组(26例)和重度组(18例);选取同期来医院体检的健康人群60例资料设为参照组,比较肝病组、参照组研究对象血清免疫球蛋白各指标即血清免疫球蛋白G(IgG)、免疫球蛋白A(IgA)和免疫球蛋白M(IgM)水平测得值的差异,以及肝病组轻度、中度和重度组患者的IgG、IgA和IgM水平测得值的差异。结果:肝病组患者的IgG、IgA和IgM水平测得值均高于参照组(P<0.05),肝病组中的重度组患者的IgG、IgA和IgM水平测得值均高于中度组和轻度组(P<0.05),中度组患者的IgG、IgA和IgM水平测得值均高于轻度组(P<0.05)。结论:CHB患者的血清免疫球蛋白水平均明显高于健康人群,通过检测患者血清免疫球蛋白水平有助于诊断其病情的严重程度,为治疗方案的制订和预后的判断提供参考。     

关于乙型肝炎患者免疫球蛋白检验的探讨

目的 检测乙型病毒型肝炎患者血清免疫球蛋白水平的变化及其临床意义.方法 采用罗氏诊断MODULAR DPP型全自动生化分析仪分别采用免疫比浊法测定血清免疫球蛋白(IgG、IgA、IgM),采用重氮比色法测定总胆红素;采用希森美康公司提供的CA-7000 自动凝血分折仪凝固法测定凝血酶原活动度(PTA).结果 慢性乙肝组以及慢性重型乙肝组的血清免疫球蛋白(IgG、IgA、IgM)水平明显高于对照组,差异具有统计学意义(P<0.05),慢性重型乙肝组较慢性乙肝组的血清免疫球蛋白(IgG、IgA、IgM) 水平的比较亦差异有统计学意义(P<0.05),情治疗无效患者的PTA值显著低于有效组患者,差异有统计学意义(P<0.05),而IgG、IgA、IgM和TBIL水平无效组均显著高于有效组(P<0.05).结论 血清免疫球蛋白(IgG、IgA、IgM)、血清TBIL、PTA水平的检测,可作为动态观察相应的指标来观察乙型肝炎患者在细胞免疫和体液免疫功能,反映肝功能损害程度的指标,为临床治疗及预后评估提供依据.     

细菌性痢疾患者血清免疫球蛋白及一氧化氮水平研究

目的探讨细菌性痢疾患者血清免疫球蛋白（Ig）及一氧化氮（NO）水平的变化及相互关系。方法分别采用硝酸还原酶法及透射比浊法对60例细菌性痢疾患者及60例正常健康儿童血清IgG、IgA、IgM和NO水平进行测定。结果细菌性痢疾患者急性期血清IgG、IgA、IgM水平较恢复期及正常对照组均明显降低,NO水平明显高于恢复期及正常对照组。急性期血清IgG、IgA、IgM水平均与NO呈显著负相关。结论血清IgG、IgA、IgM、NO与细菌性痢疾的发生、发展密切相关。     

探讨乙型肝炎患者血清免疫球蛋白水平的变化及其临床意义

目的探讨了乙型肝炎患者血清免疫球蛋白水平的变化及其临床意义。方法回顾性分析我院2010年1月至2012年4月之间收治的102例乙肝患者按照病情状况分为重型组和一般组,选同期健康献血的51名正常人作为对照组。测定所有患者的血清免疫球蛋白（IgG、IgA、IgM）,同时检测血清总胆红素（TBIL）、凝血酶原活动度（PTA）。结果一般组和重型组患者IgG、IgA、IgM水平均升高,这两组患者与正常组进行比较,差异均有显著性（P＜0.05）;另外,重型组与一般组进行比较,IgG、IgA、IgM水平之间差异也存在有显著性（P＜0.05）。IgG、IgA、IgM、TBIL水平恶化组明显高于好转组,差异均有显著性（P＜0.05）,另外,恶化组患者的PTA值明显低于好转组,两组比较差异有显著性（P＜0.05）。结论血清免疫球蛋白的水平变化情况对于患者的肝功能的情况能起到直接反应的作用,血清免疫球蛋白的水平变化是诊断乙肝的一项重要指标。     

四氯化碳诱导肝损伤大鼠自由基及微量元素含量的动态变化(英文)

为探讨微量元素与自由基代谢的关系,本实验采用四氯化碳诱导肝损伤的大鼠模型,研究了中毒大鼠体内自由基和微量元素含量的动态变化。结果大鼠经四氯化碳致毒后,肝组织自由基浓度增加,肝脏中Cu,Zn-SOD含量增加,存在着自由基底物对超氧化物歧化酶的诱导合成作用。肝脏出现明显的脂质过氧化增强现象,MDA浓度增加,GSH降低和GSSG升高。实验还观察到脑组织亦发生轻度脂质过氧化增强。在上述变化的同时,与自由基代谢有关的微量元素Zn、Cu、Fe、Mn和Se也发生了明显的改变,其中肝脏中Zn、Cu和Se含量显著降低,而肝Fe却明显增高,在脑组织中表现为Zn的降低和Se升高。上述结果说明微量元素改变在肝损伤时的自由基代谢中具有一定的作用。     

当白生肌膏对混合痔患者免疫球蛋白含量影响的研究

目的研究当白生肌膏对混合痔患者术后免疫功能的影响。方法混合痔手术患者共120例,随机均分为两组。试验组60例,混合痔术后以当白生肌膏纱条外敷,对照组60例,混合痔术后创面以雷夫奴尔纱条外敷。手术前1天和术后第14天空腹采集静脉血,分别测定免疫球蛋白IgG,IgA和IgM含量。结果试验组术后患者血清免疫球蛋白IgA和IgM含量与术前比较差异无统计学意义。对照组术后患者血清免疫球蛋白IgA和IgM含量均低于术前,差异有统计学意义(P<0.05)。试验组术后血清中IgG含量均低于术前,差异有统计学意义(P<0.05)。对照组术后血清中IgG含量均低于术前,差异有统计学意义(P<0.05)。结论当白生肌膏对混合痔患者术后体液免疫功能的恢复具有促进作用。     

Transport of trace elements in lenses of normal and hereditary cataract UPL rats

The multitracer technique was applied to the determination of the uptake of trace elements in the lenses of normal and hereditary cataract UPL rats to investigate the transport mechanisms of trace elements during cataract development. Be, Na, Sc, V, Cr, Mn, Fe, Co, Zn, As, Se, Rb, Sr, Y, Zr, Tc, Ru and Rh accumulate in normal and UPL cataract rat lenses. The rates of uptake of trace elements differ among species and also differ between normal and UPL rat lenses. The uptakes of V and Sr are greater in normal rat lenses, while the uptakes of Mn and Co are greater in UPL rat lenses. High concentrations of Zn are transported into normal rat lenses in comparison with other elements. However, the uptake of Se was highest in the lenses of UPL cataract rats. In addition, the difference in Se uptake between the normal and UPL rat lenses was greatest among the tested trace elements. The present study suggests that the transport characteristics of trace elements are different in the lenses of normal and UPL cataract rats. The different transport characteristics of trace elements in the lenses of normal and UPL cataract rats, especially the higher accumulation of Se in UPL rat lenses, may be implicated in cataract development.     

Comparative uptake behavior of trace elements in adult and suckling rat lens

The multitracer technique was applied to the determination of the uptake of trace elements in the lenses of adult and suckling rats to investigate the transport mechanisms of trace elements during developmental maturation. Be, Sc, V, Mn, Fe, Co, Zn, As, Se, Rb, Sr, Y, Zr, Ru and Rh accumulate in adult and suckling rat lenses. The rates of uptake of trace elements differ among each species and also differ between adult and suckling rat lenses. The uptakes of Fe and Sr are greater in adult rat lenses, while the uptakes of Se and Rb are greater in suckling rat lenses. High concentrations of Zn are transported into the lenses of both adult and suckling rats in comparison with other elements, and the content of Zn in suckling rat lens is higher than in adult lens. The present study suggests that different mechanisms depending on the stage of development act to transport trace elements into lenses.     

The distribution patterns of trace elements in the blood and organs in a rabbit experimental model of copper pollution and study of haematology and biochemistry parameters

In a rabbit model of five copper overfeeding, we investigated the distribution pattern of trace elements of copper [Cu], zinc [Zn], chromium [Cr], manganese [Mn], and selenium [Se] in blood serum, red cell, and whole blood and in the organs: brain, gallbladder, liver, intestines, heart, kidney, lung, and spleen. Furthermore, their haematology and biochemistry parameters as well as feed consumption and weight development were also performed in this study. Changes in trace element concentrations were determined by contrast and control group. Observably, the increased concentrations of Cr were observed in the heart; that of Cu, Zn, Cr, Mn in the liver; that of Cu, Cr, Mn in the intestines; that of Cu in the spleen; that of Cu in the blood serum; and that of Cu in the whole blood. But remarkably, the decreased concentrations of Mn were observed in heart; that of Zn and Se in the encephalon; that of Cr, Mn and Se in the spleen and that of Zn in the blood serum. In the gallbladder, the concentrations of Zn, Cr, Mn, and Se were also reduced. Excessive Cu was accumulated mainly in liver, intestines, and blood serum. In contrast group, only a few changes were detected in excretion of Mn, Zn, and Cr, but increased concentrations of Cu and Se compared with the control group. For the different biochemistry parameters measured, the contrast group showed changes, mainly owing to the altered activity of enzymes induced by trace element excess and imbalance. Increased concentrations of LDL were measured in contrast group, while a substantial decrease was seen in TG and VLDL as a result of excessive Cu. Regarding haematological parameters, increased concentrations of NEUT% and EO% were found in contrast group, but considerably decreased concentrations were detected in PLT, MONO%, BASO%, MONO#, BASO#, and P-LCR, but a minor decrease was also seen in EO#. The present paper shows the effect of copper pollution in the animals and analyzes the interplay among trace elements in their bodies at length. It is significant for environment protection and toxicology study.     

孕早期和孕中期微量元素水平与先兆流产的相关性研究

目的分析孕早、中期微量元素水平与先兆流产的相关性。方法 50例早期先兆流产患者、50例中期先兆流产患者为观察组,随机选取正常孕早、中期妇女各50例为对照组,测定微量元素锰（Mn）、铜（Cu）、铁（Fe）、锌（Zn）、硒（Se）水平。结果观察组Cu、Fe、Se水平均明显高于对照组（P〈0.05）;早期先兆流产组Zn水平明显低于正常早期妊娠组（P〈0.05）;中期先兆流产组Mn水平与正常中期妊娠组比较明显下降（P〈0.05）。结论铜、铁、硒水平与孕早、中期先兆流产密切相关,Zn、Mn水平分别为导致孕早、中期先兆流产的相关原因之一,孕早、中期孕妇应多摄入Cu、Fe、Se、Zn、Mn含量丰富的食物,避免发生先兆流产。     

白花丹中微量元素的测定

目的:建立白花丹中微量元素的测定方法。方法:采用电感耦合等离子体发射光谱(ICP-AES)法同时测定白花丹中Cu、Zn、Fe、Ca、Mg、Mn、Sr等7种微量元素,原子荧光(AFS)法测定Se元素。结果:2种方法的RSD在0.78%～3.01%之间(n=5),元素回收率在91.9%～105.8%之间,测定结果较理想。白花丹中含有非常丰富的Se元素,Cu、Zn、Fe、Ca、Mg、Mn、Sr等元素也较为丰富。结论:本方法简便、快速、准确,适于分析测定白花丹中的微量元素,测定结果对研究白花丹的药理作用具有参考意义。     

加味逍遥方中20种无机元素的分布规律及相关性研究

目的 建立加味逍遥方中无机元素的微波消解-电感耦合等离子体质谱（ICP-MS）分析方法,对不同批次加味逍遥提取物和组方药材中无机元素进行比较和分析。方法 采用微波消解法对样品进行前处理,ICP-MS法对加味逍遥提取物中铁、锌、铜、锰、镍、钒、铬、钴、硒、锶、铯、砷、镉、银、汞、铅、铀20种无机元素的进行了定量,并应用SPSS 22.0软件对结果进行统计学分析。结果 加味逍遥提取物中20种无机元素之间具有一定的相关性,应用主成分分析选出4个主因子,得出Na、Mg、Al、V、Ni、Cu、Se、Sr、Cs、Mn、Hg、Fe为加味逍遥提取物的特征元素;组方药材中主要含有Mg、Al、Mn、Fe;10味药材因其药用部位不同无机元素水平差异较大,同种元素水平从根及根茎类、皮类、全草类、果实种子类、菌类依次降低。结论 无机元素的水平变化不仅能表征提取工艺过程的稳定性,而且能为中药复方配伍规律研究提供实验依据。     

Concentration and reference interval of trace elements in human hair from students living in Palermo, Sicily (Italy)

Trace element contents in specimens of hair collected from 137 children aged 11-13 years old, living in Palermo (Sicily, Italy) were determined by ICP-MS. This work reports analytical data for the following 19 elements: Al, As, Ba, Cd, Co, Cr, Cu, Li, Mn, Mo, Ni, Pb, Rb, Sb, Se, Sr, U, V and Zn. The most abundant chemical elements were zinc and copper (Zn > Cu), with concentrations exceeding 10 μg/g (Zn = 189.2 μg/g; Cu = 22.9 μg/g). Other elements with concentrations greater than 1 μg/g were, in order of abundance, Al>Sr>Ba>Pb. The remaining elements were all below 1 μg/g. The average elemental concentrations in hair were statistically compared by Kolmogorov-Smirnov's test taking children's gender into account. Al, Ba, Cr, Li, Rb, Sb, Sr, V and Zn were statistically different according to gender, with significance p < 0.001. This study thus confirms the need for hair analysis to differentiate female data from those of males. IUPAC coverage intervals and coverage uncertainties for trace elements in the analysed hair samples are also reported.     

电感耦合等离子体质谱法测定不同批次良附丸中17种无机元素的含量

目的：测定不同批次良附丸中17种无机元素的含量。方法：采用微波消解法对样品进行处理,电感耦合等离子体质谱法（ICP-MS）测定中药成方制剂良附丸中无机元素含量。结果：不同批次良附丸中Na、Mg、K、Ca、Mn、Cr、Fe、Ni、Cu、Zn、As、Se、Sr、Mo、Cd、Pb、Hg平均含量（mg·kg^-1）分别为741.57,1214.16,12157.06,99.83,185.46,2.85,678.00,3.00,4.49,27.93,0.47,0.09,6.56,0.10,0.11,1.32,0.02。结论：良附丸中富含Fe、Mn、Zn、Cu等人体必需的微量元素,含有少量Ni、Se等人体必需的微量元素,Pb、Cd、Hg、As等有害元素的含量在限度范围内,不同批次的无机元素含量差异较小,为进一步研究无机元素与其药效间的内在关联奠定了基础。     

Trace elements in scalp hair of children living in differing environmental contexts in Sicily (Italy)

We present here data about trace elements in human scalp hair samples to test whether they are valuable to reflect environmental exposure and contamination by trace elements. The study compares contents of trace elements in scalp hair from a total of 336 children, aged 11-13 years old, living in various geographical areas of Sicily (southern Italy) characterized by differing environmental conditions. Nineteen elements (Al, As, Ba, Cd, Co, Cr, Cu, Li, Mn, Mo, Ni, Pb, Rb, Sb, Se, Sr, U, V and Zn) were determined by inductively coupled plasma mass spectrometry (ICP-MS). Coverage intervals (CI) formulated by the elemental composition of hair samples from the Palermo subjects were compared with the median content of hair from children living in the other study areas. Statistical analysis showed that Al, Ba, Sr and Zn concentrations differed significantly between gender, higher concentrations being observed in girls' hair. Children living close to the volcanic area had higher concentrations of As, Cr, Mn, Ni, Rb, Sb, U, V and Zn. Those living in an area with several old quarries had higher levels of Al, As, Pb, Rb and U. The hair of children living near the Pace del Mela industrial area contained higher levels of As, Ba, Mn, Pb, Rb, Sr and U. Linear discriminant analysis (LDA) between Palermo and the other sites allowed to better assess which variables contribute towards differentiating the groups. Our observations suggest that human hair can be used to monitor exposure to several metals, provided that sampling and analytical procedures, together with statistical treatment of data, are carried out according to standardized protocols.