Expression of transferrin receptor in normal human skin, psoriatic skin and various skin tumors

The distribution of transferrin receptor in normal human skin and its expression in psoriatic skin and various skin tumors have been investigated. Immuno-peroxidase staining was performed on biopsy specimens using monoclonal OKT 9 antibody, which reacts with transferrin receptor. Normal human skin showed positive staining with OKT 9 in eccrine glands and outer root sheaths of the hair. Sebaceous glands were also strongly positive. The basal layer stained very weakly. Psoriatic skin expressed OKT 9 strongly in the epidermis, especially in the area of the rete ridge. In squamous cell carcinoma, Bowen's disease, and malignant melanoma, a widespread and strong labelling reaction was found. Basal cell epithelioma and genital Paget's disease were partially and moderately positive in their staining pattern. No such positive staining pattern could be found in either nevus cell nevi or seborrhoic keratosis. These findings indicate that, in normal skin, transferrin receptor exists in eccrine glands, sebaceous glands, and outer root sheaths of the hair in greater amounts. High amounts of expression of this receptor in psoriatic epidermis and malignant tumors suggests that immunohistochemical demonstration of transferrin receptor parallels the proliferating activity of the tissue or tumor of the skin and may provide an useful aid for detecting such conditions.     

C3d在大疱性类天疱疮患者皮损组织中的表达

目的 探讨C3d在石蜡包埋的大疱性类天疱疮患者皮损组织中的表达及临床意义。方法 免疫组化SP法在25例大疱性类天疱疮、10例大疱性表皮松解症及10例正常成人皮肤组织标本中进行C3d、IgG、IgA进行检测,并对其在大疱性类天疱疮皮损中阳性率进行比较。结果 大疱性类天疱疮皮损中C3d、IgG、IgA的阳性率分别为96%、72%、0%。C3d、IgG在BP组织中表达阳性率的差异有统计学意义（χ2 = 4.17,P < 0.05）,C3d、IgA在BP组织中表达阳性率的差异有统计学意义（χ2 = 22.04,P < 0.01）。C3d、IgG、IgA在10例EB及正常成人皮肤组织标本中表达均为阴性。结论 免疫组化方法检测C3d的表达可以协助在石蜡组织中进行大疱性类天疱疮的诊断。     

Photodynamic DNA-breaking activity of serum from patients with various photosensitivity dermatoses

Various drugs and chemicals break the DNA strand under ultraviolet irradiation. This study aimed to clarify the DNA-breaking activity (DBA) of serum from 39 patients with various photosensitivity disorders and that from eight normal subjects. A mixture of serum and circular plasmid DNA was exposed to longwave ultraviolet radiation, and the photoinduced cleavage of plasmid DNA was examined by electrophoretic analysis. DBA was found in serum from patients with erythropoietic protoporphyria (2 of 2), drug-induced photosensitivity (3 of 5), chronic actinic dermatitis (1 of 12) and hydroa vacciniforme (1 of 1). DBA was not found in serum from patients with porphyria cutanea tarda, collagen diseases with photosensitivity, papulovesicular light eruption or pellagra. The inhibition profile of DBA by active oxygen scavengers was different between afloqualone- and tetracycline-induced photosensitivity and chronic actinic dermatitis. The present method was useful for the detection of serum phototoxicity and the investigation of the pathomechanisms of photosensitivity.     

Increased C5a anaphylatoxin in the sera of psoriatic patients and patients with inflammatory dermatoses

We measured C5a and C5a des arg (collectively called C5a) by radioimmunoassay in the sera of psoriasis and other widespread inflammatory dermatoses. Larger amounts of serum C5a were detected in patients with psoriasis and other inflammatory dermatoses than in normal healthy controls. In the psoriatic patients, the serum C5a concentrations did not correlate with extent or with activity of the skin lesions. However, when followed in 6 psoriatic patients, the C5a levels showed changes in parallel with the skin lesions. Our results suggest that complement activation with resultant release of C5a anaphylatoxin takes place in psoriasis as well as in other widespread inflammatory skin disorders.     

1 195例过敏性皮肤病变应原皮肤点刺试验结果分析

目的:了解常见的4种过敏性皮肤病的变应原分布情况。方法:采用皮肤点刺法对1195例过敏性皮肤病患者进行变应原测试。结果:慢性荨麻疹的阳性率最高。最常见的食入性变应原是海虾、海蟹、带鱼、芝麻、花生,吸入性过敏原是蟑螂、粉尘螨、室内尘、油菜花粉、柳树。结论:皮肤点刺试验可以帮助患者找出致病原,从而避免接触致病变应原。并为脱敏治疗提供依据。