绍兴地区男性不育患者精液质量分析

目的 分析绍兴地区男性不育患者精液质量。方法 选择2020年在本院就诊的167例男性不育者及214例健康查体者,比较两组间精液常规参数的差异性。结果 两组在年龄、禁欲天数、精液量、精液液化时间及液化状态间比较,差异均无统计学意义（P>0.05）;对两组（共381例）的精液量和禁欲时间进行相关性分析,相关系数r=0.18,存在正相关,即随着禁欲时间的延长,精液量随之增加;不育组中精子密度、精子活力、精子活率均显著低于健康查体组,差异有统计学意义（P<0.001）,其中不育组a级精子数下降最为明显。结论 2020年绍兴地区不育男性精液质量以精子密度、精子活力和精子活率减低为主,其中a级精子数减少最为明显,而与年龄、禁欲时间、精液量无关。不良的生活方式、不健康的生活习惯以及环境质量下降等可影响精液质量。     

男科实验室精液分析的质量控制

随着男性不育症的增加,精液分析已成为评价男性生育功能的最基本的常规测试,同时自动化分析仪的推广,计算机辅助精液分析具有较好的可靠性、重复性和稳定性。     

精液分析中精子密度室内质量控制方法研究

目的建立一种可行的精液密度测定的质量控制方法.方法采用血细胞计数板和计算机辅助精液分析(CASA)两种方法,对所选取的与精子头部直径相近的质控珠进行高、中、低三种密度测定,分别于日内及日间测试12次.结果采用血细胞计数板方法测试高、中、低三种密度结果的日内变异系数分别为12.09%、8.86%、4.72%,日间变异系数分别为16.71%、10.65%、10.22%.CASA法日内变异系数分别为6.65%、5.93%、3.80%,日间变异系数分别为7.90%、6.84%、6.14%.结论质控珠可用于以上两种测试精子密度方法的室内质量控制,从而达到保证实验室测试精子密度质量的目的.     

原发性男性不育症62例精液分析

目的对62例男性不育患者的精子进行实验室检查,探讨男性不育症患者精液质量。方法采用清华同方精子/微生物动（静）态图像分析系统（荧光版5.0）进行精子检测分析。结果精子密度〈20百万/ml者占33.33%,精子活力（运动Az级）〈25%者占93.65%,精液中白细胞〉2HPF者占55.56%。结论 93.65%的患者存在精子质量问题,表明精子质量下降是导致原发性男性不育症的重要原因。     

原发性男性不育症62例精液分析

目的 对62例男性不育患者的精子进行实验室检查,探讨男性不育症患者精液质量.方法 采用清华同方精子/微生物动(静)态图像分析系统(荧光版5.0)进行精子检测分析.结果 精干密度＜20百万/ml者占33.33%,精子活力(运动Az级)＜25%者占93.65%,精液中白细胞＞2HPF者占55.56%.结论 93.65%的患者存在精子质量问题,表明精子质量下降是导致原发性男性不育症的重要原因.     

乌鲁木齐地区483例男性不育患者精液分析

目的探讨彩色精子质量分析系统在男性不育的诊断与疗效评估中的应用价值。方法应用WUY-9000伟力彩色精子质量分析系统对乌鲁木齐地区483例不育男性和240例生育男性精液从精液量、精予总数、精子密度、精予活力、精子活率及各种精子动态参数进行研究和分析。结果不育组在精液量、酸碱度两项常规参数方面，与正常生育组相比较，两组间无显著性差异（P〉0．05），但在精子总数、精子密度（包括a、b、c、d级精子总数）、精子活率、A级精子率及B级精子率等方面，两组间有显著性差异（P〈0．01）。不育组STR、LIN略低于正常生育组，两组间比较有显著性差异（P〈0．05），VSL、VCL、VAP、ALH4项精子动态指标均呈显著性降低，两组之间也具有显著性差异（P〈0．01），而WOB、BCF、MAD3项指标与正常生育组比较。两组间无显著性差异（P〉0．05）。结论采用彩色精子质量分析系统对不育患者的精液进行多项常规和动态参数分析，可为临床上男性不育症的诊断和治疗提供有力的参考依据，也为生殖医学度优生学等相关科研工作提供了重要的研究工具。     

精液白细胞含量与精液质量主要参数的关系

目的探讨精液白细胞含量与精液质量主要参数的关系。方法依据世界卫生组织（WHO）的相关标准,精液中白细胞〉1×10^6/ml可诊断为白细胞精子症,据此分为白细胞精子组和精液白细胞≤1×10^6/ml的正常对照组。应用伟力彩色精子质量分析系统检测男性精液量、液化时间、精子总数、精子密度、精子活力、精子活率等参数进行分析。结果当精液中白细胞〉1×10^6/ml时,精液的主要参数液化时间、精子总数、精子密度、a级精子百分率、a＋b级精子百分率、精子活率与正常对照组比较差异具有显著性（P〈0.05）。结论精液中白细胞含量与精液质量有密切的关系,是导致男性不育的重要因素。     

2165例男性不育患者精液分析

目的探讨精液分析各参数在男性不育的诊断与治疗中的临床应用价值.方法采用WLJY-9000伟力彩色精子质量分析系统对2165例男性不育患者与58例已生育的正常健康体检男性的精液标本进行分析.结果不育组精液量、pH值、STR、LIN、WOB、MAD略低于生育组,而ALH、BCF略高于生育组,但均无显著性差异(P＞0.05).与生育组比较,不育组30min内完全液化者率、精子密度、A级精子率、B级精子率、精子活率、VCL、VSL、VAP均呈显著性降低(P＜0.01),而畸形精子率、WBC ～ 者率、无精子症者率均呈显著性升高(P＜0.01).结论采用伟力彩色精子质量分析系统对不育患者的精液进行多项参数分析,可为男性不育的诊断和治疗提供有力的临床依据.     

男性孕前精液质量检测结果分析

目的了解已婚未育孕前体检男性精液质量状况。方法应用WLJY-9000伟力彩色精子质量分析系统对576例已婚未育孕前体检男性精液量、液化时间、精子总数、精子密度、a级精子百分率、b级精子百分率、a＋b级精子百分率、精子活率等方面进行分析。结果已婚未育孕前体检男性的精液量、液化时间、精子总数、精子密度、b级精子百分率、a＋b级精子百分率、和精子活率与正常对照组之间差异无显著性（P〉0.05）;a级精子百分率显著降低（P〈0.05）结论随着全民教育和生活水平的提高,孕前人群的生殖健康知识认知情况很好,比较重视孕前检查。     

绍兴地区281例男性婚检者精液常规检测结果分析

目的了解281例绍兴地区婚前体检人员男性精液质量状况。方法应用WLJY-9000伟力彩色精子质量分析系统对281例男性婚检人员精液的量、外观、粘稠度、pH值、液化时间、精子密度、活率、活动力进行分析。结果281例婚检者,精液量异常的为25例,占8．9％,液化异常31例,占11．0％,pH值异常36例,占12．8％,精予密度异常48例,占17．1％,精子活率异常29例,占10．3％,精子活动力a级〈25％或（a＋b）级〈50％共有56例,占19．9％。结论婚检人群的生殖健康知识知晓情况较差,全民应重视婚前检查,进一步提高人口素质及优生优育。     

Semen quality of 324 fertile Japanese men

BACKGROUND: A number of studies have indicated regional differences in semen quality. To examine the current status in Japan, we undertook a cross-sectional study on the semen quality of fertile Japanese men for comparison with recent European results. METHODS: Semen parameters of 324 fertile men from the Kawasaki/Yokohama area were investigated. The semen parameters were compared with those published for fertile men from four European cities, Copenhagen, Paris, Edinburgh and Turku. RESULTS: When adjusting for confounders such as ejaculation abstinence period and age, the lowest sperm concentrations were detected in men from Kawasaki/Yokohama followed by men from Copenhagen, Paris, Edinburgh and Turku, but only the differences between men from Kawasaki/Yokohama and men from Edinburgh and Turku were significant (P=0.0008 and P<0.0001, respectively). Total sperm count, percentage of motile sperm and percentage of normal sperm observed in Kawasaki/Yokohama were significantly lower than those from all European centres except for motile sperm in men from Paris. CONCLUSIONS: Japanese fertile men had a semen quality at the level of Danish men, who have been reported to have the lowest among investigated men in Europe. The low level of semen quality of the fertile Japanese men may be due to lifestyle or other environmental factors; however, ethnic differences caused by different genetic variation or combinations cannot be ruled out by this study.     

4479例不孕夫妻男性患者精液检测结果分析

目的研究近年来云南省男性生殖力的变化。方法使用计算机辅助精子分析(CASA)技术,按照WHO技术规范,对自2012至2016年到我生殖中心就诊的4479例不孕症夫妻男性患者的精液进行精液常规检测,并对其结果进行统计与分析。结果4479位男性患者的平均年龄为34岁;2012~2016年间我省男性精液常规中精液量、快速前向运动精子、前向运动精子及精子活动率4项参数变化不明显;精子密度及精子总数2项参数下降趋势明显。结论我省男性患者精液部分参数下降趋势明显。     

某医院男性精液检查结果分析

目的 通过对我院男性精液检查的分析,了解本地区男性精液质量情况,为男性不育的临床诊疗提供依据。方法 采用伟力WLJY-9000型精子分析仪对308例精液进行分析,对异常指标分类统计,并根据年龄进行分组,比较各组精液量,液化时间,pH值,精子存活率,活力,精子密度,形态和各年龄组间精液量,精子密度,存活率,活力。结果 308例检查结果中,精液完全正常者179例,占58.12%,无精子症者3例,占0.97%,至少一项低于参考值者126例,占40.91%,其中精子活力异常占比最高;精液量方面:≥40岁组的为（2.85±1.13）ml,低于≤29岁组的（3.36±1.17）ml和30~39岁组的（3.41±1.21）ml,差异有统计学意义（P＜0.05）;精子存活率和活力方面随年龄有下降趋势,差异有统计学意义（P＜0.05）;精子密度方面,各组间比较,差异无统计学意义（P＞0.05）。结论 本地区男性精子活力、存活率、精子密度、精液量等指标异常所占比例较高;年龄对精液质量有一定影响,40岁以上人群精液质量下降。     

Semen quality in a residential, geographic and age representative sample of healthy Chinese men

BACKGROUND: Population-based study of semen quality is rare in literature. METHODS: Healthy men aged 20-60 years from six Chinese provinces were invited to participate in the study between December 2000 and November 2002. Posters were distributed in the participating counties to enroll 200 subjects from each province. Medians, percentiles, and proportions below lower threshold of the WHO criteria for semen parameters were calculated. Generalized linear models were used to examine the determinants of semen quality. RESULTS: Semen samples from 1191 healthy Chinese men were collected and analysed. The medians (5th and 95th percentiles) were 2.3 ml (1.0-4.5) for semen volume, 65 x 10(6)/ml (20-150) for semen concentration, 154 x 10(6)/ejection (29-421) for sperm count, 19% (5-32) for rapid progressive motility, 46% (29-66) for progressive motility, 67% (47-81) for total motile spermatozoa, 70% (48-88) for sperm viability and 39% (23-76) for normal morphology. Many healthy Chinese men had semen parameter values below the lower threshold of the WHO criteria. Region, age, abstinence duration and season were important determinants of semen quality. CONCLUSIONS: Chinese men have lower values of semen parameters according to WHO standard, and a lower threshold for normal semen parameters for Chinese men should be considered.     

慢性乙肝患者的精液结果分析

目的探讨乙肝感染对男性精液的影响。方法将不同乙肝标志物模式男性的精液参数与乙肝未感染男性进行比较。结果乙肝大三阳男性的精子畸形率高于乙肝未感染者。结论男性感染HBV会影响精子的畸形率，但是对辅助生殖技术结局的影响有待更深入的研究。     

Comparison of assays to detect cytomegalovirus shedding in the semen of HIV-infected men

We sought to determine the optimal assays for cytomegalovirus (CMV) shedding in semen. Over a 2-month period, 149 HIV-1-infected men who have sex with men each provided up to three semen specimens. Specimens were tested for CMV by culture, rapid assay (shell vial) and polymerase chain reaction (PCR). By culture, 30% of seminal plasma and 28% of seminal cell specimens grew CMV. By rapid assay, results were 38 and 33%, respectively. By PCR, 56% of seminal cell specimens demonstrated CMV: 20% in a single semen specimen; 33% in two specimens; and 34% in all three specimens. Overall, 69% of men had CMV detected by PCR in at least one seminal cell specimen. By quantitative PCR, 14% had ten, 14% had 100, 16% had 1000, and 12% had 10 000 copies in 6.25 μl of semen analyzed. Adjusting for initial CD4+ cell count, men with CMV shedding demonstrated by PCR at the first visit were approximately four times as likely to shed CMV at a subsequent visit (RR 4.28, CI: 2.30–7.95). CMV shedding was associated with decreased CD4+ cell counts in peripheral blood (P=0.05). It is concluded that the PCR assay provided the greatest sensitivity among the three detection methods.     

司机职业与男性精液质量的相关性研究

目的探讨乌鲁木齐地区司机职业与男性精液质量的相关性。方法对157例不育男性(司机组72例、非司机组85例)和125例正常生育男性精液应用WLJY-9000精子质量检测系统从精液量、液化时间、精子总数、精子密度、a级精子百分率、b级精子百分率、a+b级精子百分率、精子活率等方面进行分析。结果(1)不育司机组、不育非司机组与正常对照组比较,精子总数、精子密度、精子活力、精子活率等参数显著降低(P<0.05~0.01),液化时间延长(P<0.05)。(2)不育司机组与不育非司机组相比,精液量、精子活力、精子活率显著降低(P<0.05~0.01)。(3)不同驾龄组与不育非司机组相比,a级精子百分率、a+b级精子百分率和精子活率均显著降低(P<0.05~0.01)。另外,>10年组与不育非司机组相比,精液量显著降低(P<0.01),液化时间延长(P<0.05)。(4)>10年组与0~5年组和6~10年组,a级精子百分率、a+b级精子百分率和精子活率等参数方面均有显著性差异(P<0.05~0.01),而0~5年组与6~10年组相比各参数之间无显著性差异(P>0.05)。(5)>10年组与6~10年组相比,b级精子百分率、...     

Evolution of semen quality in North-eastern Spain: a study in 22,759 infertile men over a 36 year period

A retrospective study was conducted in a large population to determine whether sperm quality has changed in Northeastern Spain between 1960 and 1996. From a total initial population of 22,759 men, two separate groups were studied: men with spermatozoa (n = 20,411) and those with azoospermia (n = 1364). After adjustment for age and sexual abstinence, multiple linear regression analyses were used to assess changes in semen parameters over time. A 0.2% decline was observed in semen volume in the spermatozoa group (P < 0.001). No significant increase (0.04%) in sperm count (x 10(6)/ml) was observed in the spermatozoa group. There was a 0.4% increase in motile spermatozoa in the spermatozoa group (P < 0.001). There was a statistically significant decline in normal spermatozoa (3.6%) in the spermatozoa group (P < 0.001). Of the total population, 1364 men had azoospermia (6.0%). The changes observed in the semen parameters analysed in this large population showed no evidence of a deteriorating sperm quality, although a statistically significant decline was observed in the percentage of normal spermatozoa.     

中山地区男性不育患者精液质量分析

目的对中山地区男性不育患者的精液进行常规分析,了解中山地区男性不育患者精液质量的现状。方法按照《世界卫生组织人类精液检查与处理实验室手册》（第5版）的标准,应用西班牙人类精液分析微机辅助智能系统（CASA）对2012年10月～2013年6月来中山市博爱医院生殖中心就诊的2224例不育男性患者的精液进行常规检测和采用改良巴氏染色法分析精子正常形态率。结果2224例不育男性精液中正常者1113例（31．61％）,异常者1521例（68．39％）,其中液化异常964例（43．35％）,pH值异常124例（5．58％）,精子总数异常1002例（45．05％）,精子浓度异常556例（25．0％）,精子存活率异常1031例（46．36％）,精子总活力（PR＋NP）异常1208例（54．31％）,无精子症372例（16．72％）,精子正常形态率异常1021例（45．91％）。结论精子总活力、存活率下降,精子总数减少,精子正常形态率下降和精液液化不良是引起中山地区男性患者不育的主要原因,临床上应加于重视。