人乳头瘤病毒基因型检测及其人群分布特征

目的 探讨河源地区女性感染人乳头瘤病毒（HPV）基因型分布,为预防HPV感染及临床诊治提供实验依据.方法 采用导流杂交技术对6745例宫颈脱落细胞标本行人乳头瘤病毒（HPV）分型检测.结果 检出HPV感染1 701例,总阳性率为25.20％,其中单一感染1 265例（74.40％）,多重感染436例（25.63％）,又以二重感染和单独高危型复合感染较为多见.六重感染和九重感染仅见于≤24岁年龄组,50岁以上年龄组均未见五重以上感染.HPV总阳性率、高危型HPV阳性率和低危型HPV阳性率均呈现“U”型特异性分布.受检21种亚型均被检出,常见的3种高危型为HPV52（25.69％）、HPV16（17.34％）和HPV58（15.52％）,2种低危型为HPV81 （8.23％）和HPV6 （6.94％）.各年龄组中最高感染率型别也有所差异.结论 河源地区受检女性HPV的感染率较高（25.20％）,多重感染者占25.63％.HPV总阳性率、高危型HPV阳性率和低危型HPV阳性率的年龄分布存在双峰现象,出现在≥60岁和≤24岁年龄组.最常见亚型是HPV52,不同年龄组HPV感染亚型分布略有不同.     

1049例妇科门诊病人HPV感染基因型分析

目的研究宝安区妇女感染HPV的优势亚型,为宫颈癌防治提供依据。方法利用HPV基因芯片检测技术检验我院妇科门诊样本HPV亚型。结果 1049例病人中,HPV感染264例,阳性率25.17%。其中高危型（HPV16、18、31、33、35、39、45、51、52、53、56、58、59、66、68、73、83、和MM4）162例,占感染者81.44%;低危型（HPV11、6、42、43）49例,占感染者18.56%。二组感染率比较差异有统计学意义;264例感染HPV优势亚型HPV52,其次依次为16、43、58、68、18、53型;〈30岁及≥30岁两年龄段HPV感染率比较差异无统计学意义;组间优势亚型分布比较HPV53在〈30岁年龄组明显增加,其他优势亚型分布比较差异无统计学意义;单一亚型感染239例,占90.52%。结论高危亚型、单一亚型为主要常见感染类型;HPV基因分型诊断在宫颈癌筛查和防治中具有重要意义。     

134例宫颈上皮内瘤变患者HPV分型检测结果分析

目的探讨宫颈上皮内瘤变(CIN)中不同类型人乳头瘤病毒(HPV)的感染及分型情况。方法回顾性研究北京军区总医院妇科门诊134例已经确诊的CIN患者,采用凯普的核酸分子快速导流杂交基因芯片技术对HPV进行分型。结果 1.各级别CIN以HPV高危亚型单一或者多重感染为主;2.在HPV阳性的101例患者中,单一感染者73例(72.28%),双重感染者24例(23.76%),三重感染者3例(2.97%),四重感染者1例(0.99%);3.HPV-16、58感染率最高,分别为30.83%,24.06%。结论 HPV感染以高危型和多重感染为主;随CIN级别的增高,HPV阳性率也是增高的;HPV-16亚型的感染率最高。     

北京天坛医院5512例女性HPV感染基因型分析

目的 探讨北京地区女性生殖道人乳头瘤病毒(HPV)感染的基因型分布情况,为预防HPV感染和宫颈疾病防治提供理论依据.方法 使用核酸分子快速导流杂交基因芯片技术,对5512例女性宫颈脱落细胞进行HPV亚型检测.结果 892例患者感染HPV,总感染率为16.2％.居前5位的HPV基因亚型依次为HPV52、58、16、CP8304和HPV53.单一感染664例(24.1％),占所有感染的74.4％(664/892);双重亚型感染175例,占所有感染的19.6％ (175/892);多重亚型感染53例,占所有感染的5.94％(53/892).HPV感染以≤45岁的年轻女性为主(65.4％),≤25岁患者HPV感染率最高(23.2％).结论 HPV52、HPV58、HPV16、CP8304和HPV53感染在北京地区最为常见,总体符合亚洲人群分布规律,同时又具有独特的地域分布特点.     

广东某地区妇女高危型HPV感染特点及年龄结构分布

目的通过对门诊患者人乳头瘤病毒（HPV）基因型检测,分析本地区高危型HPV感染特点以及年龄构成分布。方法收集了8681例样本,采用导流杂交基因芯片技术进行21种HPV亚型基因型的分型检测。结果HPV总感染率为22．0％,高危型感染率为16．08％,以16亚型最多见（27．42％）,其次是52亚型（21．82％）;高危型HPV感染以单一型为主（78．58％）,多重型感以双重感染多见;不同年龄段的HPV感染率差异有统计学意义（xz=302．88,P〈0．01）,不同年龄的高危型HPV感染率差异亦有统计学意义（x2=321．60,P〈0．01）。结论广东该地区高危型HPV感染率略高,且具有明显的年龄分布异质性,提示预防性疫苗的使用应考虑年龄因素。     

双色荧光定量PCR快速分型、定量高危型人乳头状瘤病毒

目的针对高危型人乳头状瘤病毒（humanpapillomavims，HPV）亚型的多样性以及感染病毒载量的高低，旨在建立高危型HPV的定量与分型的快速检测方法，为HPV筛查与治疗提供依据。方法利用2种荧光染料分别标记HPV-16、HPV-33／52／58／67和HPV-18／45、HPV-31探针，同时以B球蛋白基因作为内对照，对120份疑为HPV感染的宫颈脱落细胞样本进行I{I＇V分型与定量，定量范围为5×10^1-5×10^7拷贝/ml，以HC-2杂交捕获法作为“金标准”，评价该方法的特异性与灵敏度。结果荧光定量PCR阳性检出率为52．5％（631120），其中HPV-16、HPV-18／45、HPV-31和m．V-33，52，58，67阳性率分别为36．51％（23／63）、11．11％（7／63）、12．70％（8／63）、58．73％（37／63）；HPV感染的平均病毒载量为1．08×10^7拷贝/ml。荧光定量PCR的特异性为100％，灵敏度为81．82％。结论双色荧光定量PCR能快速分型、定量高危型HPV，可用于HPV感染的筛查与宫颈病变程度预测以及疗效观察。     

北京市双桥医院妇科门诊患者人乳头瘤病毒感染情况调查

目的了解北京市朝阳区双桥医院妇科门诊不同年龄女性患者人乳头瘤病毒（human papilloma virus,HPV）亚型感染情况。方法采用核酸分子快速导流杂交技术,对2009—2012年835例受检者宫颈分泌物进行HPV—DNA亚型检测。结果538例女性患者中共检测到175例HPV阳性病例,其中高危亚型170例,低危亚型5例。高危亚型检出率居前5位依次为HPV-16（31．4％）、52（20．0％）、58（17．1％）、56（14．3％）、66（14．3％）。≥40岁以上年龄组HPV阳性率显著高于40岁以下年龄组。结论高危型HPV-16、52、58、56、66亚型是北京市双桥医院人乳头瘤病毒感染的主要亚型,40岁以上人群是防治的重点。     

徐州地区女性宫颈感染人乳头瘤病毒基因亚型分析

目的：了解徐州地区不同年龄段女性生殖道人乳头瘤病毒（HPV）感染状况及其分布规律,为徐州地区HPV分子流行病学研究提供依据。方法：以2009年2月～2009年5月来我院就诊的1299例可疑患者为对象,采集其阴道宫颈分泌物标本。用凯普医用核酸分子杂交系统（简称H ybriMax）进行21种HPV（8304、6、11、16、18、31、33、35、39、42、43、45、44、51、52、53、56、58、59、66、68）基因型的分型检测,分析常见感染亚型和分布规律。结果：HPV阳性总检出率为26.02%（338/1299）,高危型主要为HPV16,其余依次为HPV58、52、68、33、53、18感染。低危型主要为HPV11、6、8304感染;〈35岁、35～44岁、〉44岁各年龄组中HPV阳性率分别为26.92%、26.79%、22.27%;宫颈癌患者以HPV16感染为主。结论：HPV6、11、16、58、52、68、33是徐州地区HPV感染的主要型别;不同年龄段HPV阳性率无显著性差异（P〉0.05）;对HPV阳性者进行定期跟踪是防治宫颈癌的重点。     

HIV合并HPV阳性标本中HPV的型别检测

目的探讨深圳地区人类免疫缺陷病毒（HIV）阳性人群中人乳头瘤病毒（HPV）感染分型情况,为HIV阳性人群中HPV感染的防治提供依据。方法运用荧光PCR方法和反向斑点杂交技术对HPV阳性患者进行HPV分型检测。结果在HIV感染者中。利用反向点杂交分型方法对40例HPV阳性标本进行HPV基因分型。其中单型感染有18例（45．0％）,混合感染有19例（47．5％）,共检出16种HPV型别,其中包括11种高危型（16、18、31、33、35、45、52、56、58、68、73型）和5种低危型（6、11、42、44、54型）。在16种型别中,感染率最高的为16型（25．0％）,其次为52型（17．5％）、58型（15．0％）。结论感染HIV人群中生殖器部位HPV的感染率较高,在HIV阳性人群中检出性病相关的高危HPV16／52／58亚型．对HIV阳性人群中HPV感染的防治有重要指导意义。     

北京市北苑地区女性HPV感染现状及基因型分布研究

目的 调查我院辖区范围内即北京市北苑地区成年女性人乳头状瘤病毒(HPV)感染现状及亚型分布情况.方法 采用核酸分子快速导流杂交基因芯片分型技术对本院辖区范围内(北苑地区)来我院妇科门诊就诊的14582例女性患者采集宫颈脱落细胞标本进行HPV及21种亚型检测.结果 ①HPV感染阳性检出2419例,感染阳性率16.59％(2419/14582),在HPV感染阳性人群中,以单一高危型HPV感染为主,单一感染HPV阳性1951例,占HPV阳性感染人群80.65％(1951/2419);多重感染以二重感染为主,多重感染HPV阳性468例,二重感染HPV阳性361例,占HPV阳性感染人群14.92％(361/2419),以高危型感染以为主.②各HPV基因亚型检出总和为3035项次,高危型HPV感染占91.73％(2784/3035),排在前五位由高到低依次是HPV16(17.36％,527/3035)、HPV52(11.89％,361/3035)、HPV58(11.80％,358/3035)、HPV53(8.27％,251/3035)、CP8304(7.05％,214/3035),以HPV16型为主;低危型HPV感染占8.27％(251/3035),由高到低依次是HPVl1 (3.95％,120/3035)、HPV6(3.57％,108/3035)、HPV44(0.43％,13/3035)、HPV42 (0.16％,5/3035)、HPV43 (0.16％,5/3035),以HPV11为主.③按年龄段划分,≤20岁年龄组阳性率最高,阳性率31.30％ (36/115);其次是≥61岁年龄组,阳性率21.05％ (64/304).结论 本院辖区范围内(北苑地区)成年女性HPV感染阳性率16.59 ％(2419/14582),以单一高危型HPV感染为主,高危型HPV16、HPV52、HPV58、HPV53、CP8304是本院辖区范围内成年女性HPV感染的主要亚型.     

宫颈分泌物HPV16,18型的FQ-PCR检测对育龄夫妇生殖健康的意义

目的探讨人乳头瘤病毒（HPV）16,18亚型在河北省衡水市育龄妇女中的流行情况以及感染妇女的男性性伴侣的感染率。方法采用荧光定量PCR（FQ-PCR）技术对367名育龄妇女进行了HPV16,18检测。观察HPV16,18感染的年龄分布特点。随后,30名HPV16,18阳性妇女和30名阴性妇女的男性性伴侣被邀请进行了HPV16,18检测。结果本地区HPV16,18现患率为10.4%（38/367）。感染妇女其男性伴侣感染率为13.3%（4/30）,与对照组非感染妇女的男性伴侣的感染率0（0/30）相比,有显著性差异（P=0.019,单侧检验）。结论367例育龄妇女分泌物HPV16,18检测阳性率为10.4%,男性性伴侣的感染率为13.3%。     

Cervical human papillomavirus among women in Guangdong,China 2008-2017: Implication for screening and vaccination

Our study aimed to analyze genotype-specific, age-specific prevalence, and year-on-year trend of cervical human papillomavirus (HPV) detection among women in Guangdong, China 2008 to 2017. A total of 199 963 women attending the gynecological department and 11 999 women attending the medical examination center at Guangdong Women and Children Hospital were included. Prevalent HPV detection significantly differed between these two groups of women (20.16% vs 17.25%; P < .001). HPV genotypes of these two populations have a large overlap, with HPV52, 16, 58, CP8304, and 53 being the dominant subtypes among gynecological outpatients and HPV52, CP8304, 58, 53, and 16 among women receiving physical examinations. The distribution of prevalent HPV detection showed a bimodal pattern across age groups among these two populations. However, prevalent HPV detection among these two populations exhibited different trends from 2008 to 2017. Our study demonstrated that prevalent HPV detection among women in Guangdong is associated with age and different from that among women from other regions of China. Our study may provide valuable data to inform cervical cancer screening and HPV vaccination programs for women in this province.     

人乳头瘤病毒分型与宫颈病变关系的研究

目的探讨不同砸别人乳头瘤病毒（Human papilloma virus,HPV）感染与宫颈疾病发生发展的关系。方法应用核酸分子快速杂交基因分型技术,对375例宫颈病变患者（宫颈炎182例、CINI81例、CINⅡ68例、CINⅢ69例、宫颈癌15例）进行HPV感染基因型分型测定,同时进行宫颈细胞学检查。结果375例标本中HPV阳性检出率为35．2％,HPV在各类宫颈病变组的阳性枪出率分别为宫颈炎18．6％,CINI32．1％,CINⅡ57．3％,CINⅢ68．9％,宫颈癌86．7％,宫颈炎组和宫颈癌病变组HPV之间结果差异有统计学意义（x^2=105．0,P〈0．05）。HPV多重感染率为32．6％,其中最常见HPV感染亚型为HPV16。结论宫颈病变严重程度与感染HPV亚型的致病能力密切相关。HPV核酸分型检测对宫颈疾病诊断和治疗具有重要意义。     

A pilot study on the distribution of human papillomavirus genotypes and HPV-16 variants in cervical neoplastic lesions from Ecuadorian women

Human papillomaviruses (HPVs) are the cause of cervical intraepithelial neoplasia and invasive carcinomas of the uterine cervix. The distribution of specific HPV genotypes varies greatly across populations and HPV surveys have been performed in different geographical regions in order to apply appropriate vaccine strategies. The aim of this study was to determine the spectrum of HPV genotypes and HPV-16 variants among women with cervical lesions living in Ecuador. A total of 71 cases have been analyzed, including 32 chronic cervicitis, 29 cervical intraepithelial neoplasia grade 1, and 10 cervical intraepithelial neoplasia grade 2-3. HPV sequences were detected by broad spectrum consensus-primer-pairs MY09/MY11 and GP5+/GP6+-based polymerase chain reaction and characterized by nucleotide sequence analysis. Overall, 31 (43.7%) cases were HPV positive with prevalence rates of 37.5%, 44.8%, and 60% in patients with chronic cervicitis, cervical intraepithelial neoplasia grade 1 and cervical intraepithelial neoplasia grade 2-3, respectively. Among the positive cases, the most common genotypes were HPV 16 (64.5%) and HPV 81 (29%) followed by HPV 31, 53, 56, and 58, in descending order of prevalence. Seventeen (85%) HPV-16 isolates were classified as European and three (15%) as African-1 variant on the basis of nucleotide signature present within the MY09/MY11 L1 sequence. The results suggest that HPV 16 has a very high prevalence among women with cervical lesions in Ecuador; therefore, an effective HPV-16 based vaccine should prevent the development of cervical cancer in a large proportion of Ecuadorian women.     

Prevalence and distribution of human papillomavirus infection in korean women as determined by restriction fragment mass polymorphism assay

The development of a prophylactic vaccine that targets human papillomaviruses (HPV) 6, 11, 16, and 18 to prevent cervical cancer has increased interest in the ethnic and geographical distributions of HPV genotypes. We investigated HPV prevalence and type distribution by restriction fragment mass polymorphism (RFMP) testing a total of 60,775 specimens (aged 18-79 yr, median 44) taken from liquid-based cytology. Overall HPV positive rate of total patients was 34.2%. Among the positive patients, 87.7% was single type infections, and 12.3% was multiple HPV types. HPV-16 was the most prevalent genotype observed in 2,307 (26.0%), followed by type 52 in 2,269 (25.5%), type 58 in 1,090 (12.3%), type 18 in 633 (7.1%), type 56 in 436 (4.9%). The pattern of high risk-HPV positive rate according to age showed U-shape with a peak in HPV prevalence among women less than 30 yr of age, and a second peak among the older females aged 70 to 79 yr. The leading four high-risk HPV genotypes were HPV-16, HPV-52, HPV-58, and HPV-18 in descending order. In conclusion, this study provides the most representative prevalence and type-specific distribution of HPV among Korean women, and demonstrates that the epidemiology of HPV infection is different from that of other regions of the world.     

HPV genotype prevalence in cytologically abnormal cervical samples from women living in south Italy

Human papillomavirus (HPV) infection is the commonest sexually transmitted infection, and high-risk HPV types are associated with cervical carcinogenesis. This study investigated: the HPV type-specific prevalence in 970 women with an abnormal cytological diagnosis; and the association of HPV infection and cervical disease in a subset of 626 women with a histological diagnosis. HPV-DNA was researched by nested PCR/sequencing and the INNOLiPA HPV Genotyping assay. The data were analysed by the chi-square test (p相似文献   

渝东南妇科女人乳头瘤病毒感染状况分析

目的:了解渝东南妇女人乳头瘤病毒(HPV)感染的基因型分布特点,为该片区HPV疫苗的研发和宫颈癌的防治提供可靠而科学的依据。方法:采用聚合酶链反应(PCR)和脱氧核糖核酸(DNA)反向点杂交相结合的DNA芯片技术对来自渝东南门诊妇科的5073例就诊者进行HPV感染及分型检测。结果:HPV总感染率为37.5%(1902/5073),其中单纯低危型感染率为6.8%(344/5073),以HPV6,81为主;高危型及混合感染率为30.7%(1558/5073),以其高危型检出频次前五位依次为16(21.9%),52(18.4%),58(17.6%),33(12.5%),18(9.1%)。共检出22种基因型,没检出82型;在各年龄组中,其感染率与年龄段呈U型曲线关系,高危型感染在50岁以上明显上升(X2=15.78,P0.005)。结论:HPV感染率及基因分型有一定的地域差异,该片区感染率较高,高危型及多重感染情况比较严重,基因分型符合亚洲人的特点,但也有各自特点。     

Prevalence and genotype distribution of human papillomavirus infection among female sex workers in Guangxi, China: implications for interventions

Human papillomavirus (HPV) infection is a major public health concern in women, but information on HPV among female sex workers in China is limited. The aim of the study was to estimate the prevalence and genotype distribution of HPV infection among female sex workers in two cities in Guangxi, China. A total of 811 female sex workers were recruited from venues between July and September of 2009. Data on socio-demographic and behavior characteristics were collected, and cervical swabs were collected to determine HPV infection and genotype distribution. The overall prevalence of infection with any HPV type was 38.9%. HPV type 52 was the most prevalent type with prevalence of 11%, followed by HPV types 16, 58, 53, and CP8304, with prevalences of 6.5%, 5.7%, 5.6%, and 4.8%, respectively. HPV 16 or 18 accounted for 23.2% of all HPV positive cases. Age group <20 years was significantly associated with infection of the high-risk and multiple types of HPV infection. A higher prevalence of multiple HPV infection was observed among female sex workers from the outdoor venues (14.0%; 95%CI, 10.6%-17.3%). These findings have important implications for developing HPV prevention programs including HPV vaccination in female sex workers.