缺锌对大鼠胸腺发育影响及机理探讨

目的：研究缺锌对胸腺发育及Ｔ淋巴细胞活化与增殖功能的影响。方法：建立大鼠缺锌（ＺＤ）模型，测定胸腺肽、胸腺激素活性、胸腺Ｔ淋巴细胞转化、活化Ｔ淋巴细胞钙离子和活性钙调蛋白等。结果：１．ＺＤ组的血清、毛、胸腺细胞锌含量低于对照（ＡＬ）组（Ｐ＜０．０１）。２．ＺＤ组的胸腺重量、指数和细胞大小低于ＡＬ组（Ｐ＜０．０１或Ｐ＜０．０５）。３．ＺＤ组胸腺的胸腺肽含量、胸腺素活性低于ＡＬ组（Ｐ＜０．０１）。４．ＺＤ组胸腺细胞的ＤＮＡ、ＲＮＡ和蛋白质含量低于ＡＬ组（Ｐ＜０．０１）。５．ＺＤ组胸腺细胞中增殖期细胞（Ｓ＋Ｇ２／Ｍ）、增殖指数（ＰＩ）低于ＡＬ组（Ｐ＜０．０１）。６．ＺＤ组胸腺细胞内ｃＡＭＰ含量与ｃＡＭＰ／ｃＧＭＰ比值高于ＡＬ组（Ｐ＜０．０１）。７．ＺＤ组胸腺细胞内的Ｃａ２＋、ＣａＭ、ＩＬ－２、ＩＬ－２Ｒα及Ｔ细胞增殖率低于ＡＬ组（Ｐ＜０．０１）。８．ＺＤ组胸腺Ｔ淋巴细胞内的锌离子与Ｃａ２＋、ＣａＭ、ＩＬ－２、ＩＬ－２Ｒα、Ｔ淋巴细胞增殖率呈正相关（Ｐ＜０．０１）；Ｃａ２＋与ＩＬ－２、ＩＬ－２Ｒα呈正相关（Ｐ＜０．０１）；ＣａＭ与ＩＬ－２、ＩＬ－２Ｒα呈正相关（Ｐ＜０．０１）。结论：适量锌有促进胸腺发育、胸腺激素活?     

激光作业人员血脂调查

本文调查１３０名激光科研工作者的血脂。调查结果，激光作业人员各项血脂均值与标准差为：ＨＤＬ－Ｃ，男１．２３８±０．２４３ｍｍｏｌ／Ｌ，女１．２８８±０．２０７ｍｍｏｌ／Ｌ；ＨＤＬ－ｃ／ＴＣ，男０．３３±０．０８，女０．３４９±０．０８６；ＴＧ，男１．２２２±０．３７１ｍｍｏｌ／Ｌ，女１．２８４±０．６０２ｍｍｏｌ／Ｌ·ＨＤＬ－ｃ均值与ＨＤＬ－ｃ／ＴＣ比值明显低于对照组（Ｐ＜０．０１），ＴＧ均值明显高于对照组（Ｐ＜０．０１）。各指标异常率比较，激光组男性ＨＤＬ－ｃ、ＨＴＬ－／ＴＣ、ＴＣ与ＴＧ的异常率分别高于对照组（Ｐ＜０．０１），女性ＴＧ增高的异常率亦明显高于对照组（Ｐ＜０．０１）。结果提示激光工作者患冠心病的危险性增高。     

海藻硫酸多糖抗氧化与抗肿瘤作用的实验研究

目的：观察海藻硫酸多糖（ＳＰ）对正常及荷瘤小鼠肝匀浆及红细胞中ＳＯＤ，ＧＳＨ－Ｐｘ活性及脂质过氧化产物ＭＤＡ含量的影响，探讨其抗氧化与抗肿瘤之间的关系。结果：移植肿瘤Ｓ１８０使肝组织中ＳＯＤ，ＧＳＨ－Ｐｘ活性分别由１１３．７３±８．１７降至７１．４１±１０．９８ｍｇ／Ｌ（Ｐ＜０．０５），３２．３４±０．０５降至２９．９６±０．１６Ｕ（Ｐ＜０．０１），ＭＤＡ含量由０．２３４±０．００１增至０．２８０±０．０１２（Ｐ＜０．０５），腹腔注射或口服ＳＰ则使ＳＯＤ，ＧＳＨ－Ｐｘ活性增至１１８．７７±１８．９２ｍｇ／Ｌ（Ｐ＜０．０５），３６．５８±１．０６Ｕ（Ｐ＜０．０１），ＭＤＡ含量降至０．１０４±０．０３９（Ｐ＜０．０１）。且腹腔注射ＳＰ１００ｍｇｋｇ－１ｄ－１×１０ｄ可使肿瘤抑制率达３９．１３％，口服ＳＰ２００ｍｇｋｇ－１ｄ－１×１０ｄ也能使肿瘤抑制率达２８．０４％。结论：ＳＰ抑瘤作用的机理可能与抗氧化有关。     

广西肝病患者及献血员的HCV Serotype和Genotype的分子生物学研究

选择广西不同地市的肝病患者１０３例（ＡＨ８、ＣＡＨ３６、ＣＰＨ３６、ＬＣ７、ＨＣＣ１６）和献血员３５５例进行Ａｎｔｉ－ＨＣＶ阳性率、ＨＣＶＳｅｒｏｔｙｐｅ和Ｇｅｎｏｔｙｐｅ的分子生物学研究。结果表明：肝病患者Ａｎｔｉ－ＨＣＶ阳性１２例（ＡＨ１／８。ＣＡＨ２／３６。ＣＰＨ９／３６。ＬＣＯ／７、ＨＣＣ０／１６），阳性率为１１．６５％，明显高于同室检测兰州和南京肝病患者的Ａｎｔｉ－ＨＣＶ阳性率４．３％和４．２％（Ｐ＜０．０１）；２４０例献血员中检出Ａｎｔｉ－ＨＣＶ阳性１１例（４．５８％），明显低于同室检测兰州献血员的Ａｎｔｉ－ＨＣＶ阳性率３５．００％（Ｐ＜０．０１）；本文两组的Ａｎｔｉ－ＨＣＶ阳性率也存在显著性差异（Ｐ＜０．０１）。肝病患者组的ＨＣＶＳｅｒｏｔｐｙｅ１型、２型、１＋２型和无法定为１和／或２型者分别为３３．３３％、０、０和６６．６７％；而献血员组的ＨＣＶＳｅｒｏｔｙｐｅ分别为６５．９５％、１．１０％、２．２０％和３０．７７％。此外，肝病患者组的ＨＣＶＧｅｎｏｔｙｐｅ均属ｌｂ型（５０．００％）和ｌｂ混合型（５０．００％）；献血员组的ＨＣＶＧｅｎｏｔｙｐｅ中ｌａ型、ｌｂ型、ｌｂ混合型和无法定为１和／或２     

上珠地村地方性镉中毒的调查报告

连城县庙前镇上珠地村鸟石坪溪水、食水坑溪水水镉分别为０．０３ｍｇ／Ｌ、０．０８ｍｇ／Ｌ，是国家生活饮用水允许含量的３倍、８倍，属原生镉污染水体；农田土壤镉均值１．２８ｍｇ／ｋｇ，是对照村的６．４倍（Ｐ＜０．０１）；糙米镉均值为０．６４ｍｇ／ｋｇ，是对照村的２６．６７倍（＜０．０１），镉中位数为０．２１５μｇ／ｇ，是对照村的２．９１倍（Ｐ＜０．０１）；吸烟者全血镉几何均值为９．１０μｇ／Ｌ，是对照村的４．９３倍（Ｐ＜０．０１）；不吸烟者全血镉几何均值为５．９３μｇ／Ｌ，是对照村的６．８２倍（Ｐ＜０．０１）；尿镉几何均值为１０．４μｇ／ｇ·ｃｒ，是对照村的６．９倍（Ｐ＜０．０１）；尿镉几何均值为１０．４μｇ／·ｃｒ，是对照村的６．９倍（Ｐ＜０．０１）；尿β２－微球蛋白几何均值为１８１μｇ／ｇ·ｃｒ，是对照村的１．７４倍（Ｐ＜０．０１）；发现７例有肾小管功能障碍的象征，尿β２－微球蛋白在１１１４～１３２４８μｇ／ｇ·ｃｒ，原总蛋白也有所提高。确证该村为原生镉污染，属地方性镉中毒病区。     

维生素C对暴露于臭氧环境中小鼠心脑脂褐质水平的影响

小鼠２４ｈ暴露于低浓度臭氧环境中，１周后小鼠心、脑脂褐质含量（１０．９±１．７、８．６±１．７μｇ／ｇ）明显高于对照组小鼠心、脑脂褐质含量（８．１±１．５、６．９±１．６μｇ／ｇ）（Ｐ＜０．０１）。而给暴露在臭氧环境中小鼠每日喂以５ｍｇ／ｋｇ维生素Ｃ后，小鼠心、脑脂褐质含量（８．３±０．８、５．２±０．７μｇ／ｇ）明显低于臭氧组（Ｐ＜０．０１），与对照组相比较无显著性的差异（Ｐ＞０．０５）。     

尿毒症、慢性肾炎患者血清维生素A、C、E的研究

本研究发现尿毒症病人血清维生素Ａ含量为１２７．７０±６０．８０ｍｇ／Ｌ，显著高于正常对照组５８．００±１８．００μｇ／ｄｌ（Ｐ＜０．００１），维生素Ｅ含量则显著低于正常对照组（Ｐ＜０．０１），维生素Ｃ含量与正常无显著性差别。慢性肾炎组维生素Ａ为１１２．８１±６２．９３ｍｇ／Ｌ，显著高于正常对照组（Ｐ＜０．０１），维生素Ｅ含量与正常无显著性差别。这对于指导临床用药及病人饮食营养有一定意义。     

三硝基甲苯,铅,黄磷接触者唾液咖啡因清除率对肝毒作用的早期评价

毒物对肝脏的早期作用常表现为肝混合功能氧化酶（ＭＦＯ）的变化，咖啡因主要经肝ＭＦＯ代谢，因此测定其唾液清除率（ＳＣＬｃａ）可用于早期评价某些毒物的肝毒作用。三硝基甲笨（ＴＮＴ）、铅、黄磷接触者口服１７０ｍｇ咖啡因，８小时后收集唾液测定咖啡因含量及求出ＳＣＬｃａ，对照组（１７例）为１３４．３±５９．７ｍｌ／ｍｉｎ，ＴＮＴ接触者（３７例）为６６．５±３３．８ｍｌ／ｍｉｎ（Ｐ＜０．０１），铅接触者（３４例）为６４．１±３０．３ｍｌ／ｍｉｎ（Ｐ＜０．０１），黄磷接触者（３８例）为１４９．９±６８．７ｍｌ／ｍｉｎ。以对照组ＳＣＬｃａ９５％可信限作正常范围，三种接触者ＳＣＬｃａ的异常检出率均比ＳＧＰＴ和γ－ＧＴ的异常检出率高（Ｐ＜０．０１），凡ＳＧＰＴ和γ－ＧＴ异常者ＳＣＬｃａ大多有改变。结果表明ＳＣＬｃａ作为一种无损伤性的肝代谢功能检测方法，对ＴＮＴ、铅接触者早期肝代谢功能改变的评价有一定的意义。     

用计算机辅助精子分析系统测定TCDD对大鼠精子运动能力的影响

利用计算机辅助的精子分析系统（ＣＡＳＡ）研究了２,３,７,８—四氯二苯－Ｐ－二口恶口英（ＴＣＤＤ）对大鼠精子运动能力的影响。给２１天龄幼鼠腹腔注射ＴＣＤＤ０．１、１．０和５．０μｇ／ｋｇ,对照组注射等量体积的溶媒。在性成熟后（９０天）,用扩散法收集附睾尾精子,测定其运行速度（ＶＣＬ、ＶＳＬ、ＶＡＰ）、运动方式（ＳＴＲ、ＬＩＮ、ＢＣＦ、ＡＬＨ、ＭＡＤ等）及活动精子的比率（Ｍｏｔ％）。结果表明,ＴＣＤＤ１．０μｇ／ｋｇ组,精子的运动速度明显低于对照组（ＶＣＬＰ＜０．０５;ＶＡＰ、ＶＳＬ、ＢＣＦＰ＜０．０１）,０．５μｇ／ｋｇ时精子的运动速度、及前向性和直线性运动性能均明显降低（Ｐ＜０．０１）,活动精子的比率也明显低于对照组（Ｐ＜０．０１）。本研究结果为ＴＣＤＤ的男性生殖毒理学研究,以及ＣＡＳＡ在男性生殖毒理学研究中的应用提供了进一步参考依据     

红米对大鼠血脂及抗氧化系统的影响

了解红米对机体血脂及抗氧化系统的影响。方法：ＳＤ二级雄性大鼠３０只,体重（ｇ）１８０～２２０,随机分为两组,每组１５只。实验组：普通大鼠饲料加３０％红米粉;对照组：普通大鼠饲料加３０％白米粉,连续喂养４５天,喂养期间每天称进食量,每周测定体重,第４６天取动脉血测定血清总胆固醇（ＴＣ）、甘油三酯（ＴＧ）、高密度脂蛋白胆固醇（ＨＤＬ－Ｃ）、低密度脂蛋白胆固醇（ＬＤＬ－Ｃ）;血清谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）和超氧化物歧化酶（ＳＯＤ）活性。结果：１．两组动物体重增长和日进食量无显著性差异。２．血脂比较：红米组ＨＤＬ－Ｃ（ｍｍｏｌ／Ｌ）０．７２±０．１１明显高于白米组０．７０±０．０５,Ｐ＜０．０５。ＴＣ、ＴＧ、ＬＤＬ－Ｃ两组无明显差异。３．血清ＧＳＨ－Ｐｘ和ＳＯＤ活性：红米组ＧＳＨ－Ｐｘ活性（Ｕ）１１４．０９±４．７８明显高于白米组１０９．４５±１５．０７,Ｐ＜０．０１;红米组ＳＯＤ活性（ｎＵ／ｍｌ血清）２４８．７９±４７．５９明显高于白米组（２１０．８４±３２．０９）,Ｐ＜０．０５。讨论近年来大量研究表明,机体脂质代谢紊乱,抗氧化能力降低,氧化脂质产物增多是造成动脉硬化血管损伤的关键。随着年龄增加,人体内抗?     

番石榴叶提取物对小鼠肝脏抗氧化作用研究

目的研究番石榴叶提取物抗氧化活性。方法干燥的番石榴叶分别用蒸馏水、65%和95%乙醇浸提,浸提液过滤、浓缩、干燥得到3种提取物。测定各提取物对羟自由基清除率和脂质过氧化抑制率。紫外分光光度法分析提取物中总黄酮含量,高效液相色谱(HPLC)和紫外可见吸收光谱法(UV)对黄酮类化合物初步鉴定。结果水、65%和95%乙醇提取物均具有清除羟自由基和抑制脂质过氧化作用,且呈剂量-效应关系,对羟自由基清除作用的半数有效浓度(EC50)分别为0.63、0.47和0.58g/L,对脂质过氧化抑制作用的半数有效浓度(EC50)分别为0.20、0.035和0.18g/L,总黄酮含量分别为3.28、30.71和55.98g/kg。结论番石榴叶水和乙醇提取物具有较强的抗氧化作用,其中黄酮类化合物可能是其功效成分之一。     

Radical scavenging, antibacterial, and antiproliferative activities of Melissa officinalis L. extracts

The aromatic herb Melissa officinalis L. can be used as an easily accessible source of natural antioxidants and as a possible food supplement and as a phytochemical. Radical scavenging, antibacterial, and antiproliferative activities of petroleum ether, chloroform, ethyl acetate, n-butanol, and water extracts of M. officinalis L. extracts were investigated. The results of antioxidative activity, obtained by electron spin resonance spectroscopy, confirmed that investigated extracts suppressed the formation of 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, and lipid peroxyl radicals in all investigated systems in a dose-dependent manner. The maximum DPPH and hydroxyl radical scavenging activities (SA(DPPH) = SA(OH) = 100%) were achieved in the presence of n-butanol extract at concentrations of 0.4 mg/mL and 0.5 mg/mL, respectively. The highest lipid peroxyl scavenging activity (93.20%) was observed at a higher concentration (5 mg/mL) of n-butanol extract in the lipid peroxidation system. The most effective antibacterial activities were expressed by petroleum ether and ethyl acetate extracts on Sarcina lutea. Chloroform extract showed the strongest antiproliferative effect with 50% inhibitory concentration values of 0.09 mg/mL and 0.10 mg/mL for HeLa and MCF-7 cell lines, respectively. The present study demonstrated the high phenolic content and radical scavenging, antibacterial, and antiproliferative activities of extracts of M. officinalis L. originating from Serbia.     

子宫实验和E-SCREEN实验在检测雌激素活性中的相关性

目的　以 1 7β 雌二醇 (1 7β E2 )和四种植物提取物为受试物 ,研究子宫实验和E SCREEN实验在检测雌激素活性中的相关性。方法　断乳雌性小鼠 (1 0～ 1 2g)按体重分为 6组 ,分别给予小茴香、山豆根、补骨脂和川牛膝提取物 (1 0g kgBW ,ig)、1 7β E2 (0 5mg kgBW ,sc)和蒸馏水 (ig) ,持续 9天后处死动物剥离子宫称重。分别在人类乳腺癌MCF 7细胞培养液中加入四种植物提取物 (终浓度 1 0mg L)和 1 7β E2 (终浓度0 3μmol L) ,计算各组细胞在 1 2 0h内的平均群体倍增时间 ,分析小鼠子宫重量与细胞群体倍增时间的相关性。结果　 4种植物提取物均可使小鼠子宫重量增加 (P <0 0 5或P <0 0 1 ) ,细胞群体倍增时间缩短。子宫重量与群体倍增时间呈显著负相关 (r=- 0 96 7,P <0 0 1 )。结论　子宫实验和E SCREEN实验结果在检测雌激素活性物质时具有一致性     

Antioxidative activity of hen egg ovalbumin hydrolysates

To evaluate the antioxidative activity of the hydrolysates of ovalbumin, the antioxidative activities of the enzymatic extracts were evaluated using three different methodologies scavenging assays such as superoxide anion, hydroxyl radical, and inhibitory oxidation of linoleic acid in vitro, and the activities of SOD, GSH-PX, CAT and the level of MDA were determined in serum and liver of aged mice induced by G-gal. The results showed that the hydrolysates had a distinctly inhibitory action to superoxide anion made by alkaline pyrogallic acid, HO. produced by Fenton reaction, the oxidation of linoleic acid in linoleic acid autoxidation system, and presented a positive correlation. The inhibition capacity of hydrolysates against superoxide anion and HO. were more than 45% and 56% respectively at the concentration 5 mg/mL. And the hydrolysates could significantly (p< 0.01) prevented the activities of SOD, GSH-Px, and CAT against reducing and all three concentrations could significantly (p< 0.01) decrease the MDA contents in the serum and liver of aged mice induced by G-gal. The antioxidative activity of high concentration was similar to that of control group.     

Identification of antioxidant and antimicrobial compounds of Lippia species by bioautography

The methanolic extracts of the leaves of Lippia species (L. pseudo-thea, L. hermannioides, L. alba, L. rubella, and L. sidoides) were tested for their antibacterial, antifungal, and antioxidant activity. Cytotoxicity was determined by using brine shrimp lethality bioassay. Phytochemical screening was also performed. The extracts showed a minimum inhibitory concentration (MIC) ranging from 78 to 5000 μg/mL for antibacterial activity against at least 2 species of bacteria, although none was active against Escherichia coli. Antifungal activity was found only for L. pseudo-thea (MIC, 625?μg/mL for Candida albicans) and L. sidoides (MIC, 625 μg/mL for both C. albicans and C. neoformans). The bioautography showed that flavonoids and coumarins are responsible for the antioxidant activity of the extracts and that the antimicrobial properties are due to flavonoids and terpenoids. The cytotoxic activity was stronger for L rubella extract. To our knowledge, this is the first report of the biological and chemical constituents of L. pseudo-thea, L. hermannioides, and L. rubella.     

Nutritional Content and Antioxidant Properties of Pulp Waste from Daucus carota and Beta vulgaris

This study reports the chemical composition and antioxidant potential of pulp waste from two vegetables, carrot (Daucus carota) and beetroot (Beta vulgaris). Different in vitro assays used for determining antioxidant potential of extracts of pulp wastes were: 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity, reducing power and total antioxidant activity by phosphomolybdenum method. Total polyphenols, tannins and antioxidative components such as vitamin C, total carotenoids and β-carotene were analysed in the samples. The moisture content of samples ranged from 79 - 84%. The protein content was high in beetroot (13.23 mg/100g) and low in carrot (6.21mg/100g). Total polyphenols were higher in methanol extracts of samples (220-250 mg/100g) compared to ethanol and aqueous extracts. The antioxidant activity determined by the DPPH method exhibited 40% and 78% activity in methanol extracts of carrot and beetroot pulp waste (20 mg) respectively. Overall, the results suggest that carrot and beetroot pulp wastes can be exploited for their nutrients and antioxidant components and used for value addition in food formulations. Hence, these results pave the way for utilisation of bio-wastes from the food industry.     

枸杞多糖对小鼠耐常压缺氧能力的影响

目的　为了进一步研究枸杞耐缺氧能力与成分的关系 ,对枸杞多糖的耐缺氧效应进行了研究。方法　连续灌胃小鼠不同剂量的枸杞多糖 (LBP) 7d,末次给予后 1 h,进行密闭缺氧实验。结果　实验结果表明 ,LBP50～ 90 0 mg/(kg·d) ig,可显著延长小鼠的存活时间 ,其中以70 0 mg/(kg·d)剂量效果最佳 ,存活时间为 (47.3± 4.4) min,比对照组延长 52 .1 %。LBP可降低小鼠血红蛋白增加率 ,抑制组织脂质过氧化反应以及增加血浆中 SOD活性。结论　 LBP具有提高实验动物耐缺氧能力的功能。     

荞麦蛋白和类黄酮提取物清除自由基的ESR研究

目的:探讨荞麦蛋白质提取物(BWPr)和类黄酮提取物(BWF)清除活性氧自由基的作用。方法:采用电子自旋共振(ESR)及自旋捕获技术。结果:BWPr可清除O2,且呈明显的量效关系,在1.4～_?6.0mg/ml浓度范围内,随着蛋白液浓度的增加,清除率近乎直线升高,而再增加蛋白液的浓度,清除率增加幅度下降,甜荞蛋白和苦荞蛋白的IC50分别为4.1mg/ml、3.2mg/ml。而BWF清除O2的作用高于_?甜荞蛋白和苦荞蛋白,当其浓度为58mg/L时清除率就达77.89%,而浓度为293mg/L时清除率达到98.56%。BWPr对·OH也有一定的清除效果,但不及BWF。结论:BWPr和BWF都有清除活性氧自由基的作用,提示除荞麦类黄酮外,荞麦蛋白质也是一种自由基清除剂。     

Nutritional Composition, in vitro Antioxidant Activity and Artemia salina L. Lethality of Pulp and Seed of Tamarindus indica L. Extracts

This study was designed to examine the nutritional composition, antioxidant activity and medium lethal concentration (LC50 value) of Tamarindus indica L. pulp and seed extracts in vitro. The extraction was set at 40?C, 60?C and 100?C for 12 hours, 6 hours and 15 minutes respectively to determine the optimum extraction parameter whereas the anti-oxidant activity of the extracts was measured using iron (III) reduction (FRAP) assay. Total phenolic content (TPC) of the extracts was estimated as gallic acid equivalent by Folin-Ciocalteau method. Toxicity potential of the extract was assessed in vitro by Artemia salina lethality test both in seed and pulp samples. The results showed that tamarind seed contained a higher percentage of carbohydrate, protein, fat and energy (15%, 82%, 95% and 33.13% respectively) than the pulp. On the other hand, the pulp demonstrated a high moisture (51.1%) and ash (34.84%) content than the seed. For the mineral analysis, tamarind seed contained higher Ca and C (1.0% and 50.73% respectively) than the pulp (0.27% and 40.40% respectively). No heavy metals were detected in both samples. Seed extracted at 60?C/6 hours and 100?C/15 minutes showed the highest TPC value and were significantly different (p<0.05) than the seed extracted at 40?C/12 hours. Anti-oxidant activity is positively correlated to the TPC value of the extracts (R=0.991). The pulp and seed extracted at 100?C/15 minutes showed the highest FRAP value among its groups (216.17 ± 14.06 μmol (Fe)/g and 659.74 ± 16.40 μmol (Fe)/g respectively). This study indicates that tamarind pulp and seed extracts possess beneficial antioxidant properties and the optimum extraction parameter is 100?C for 15 minutes. In Artemia salina lethality test, tamarind pulp caused significant mortality of the crustacean larvae with LC50 in the range of 26-28 μL/mL. Tamarind seed were not toxic to Artemia salina since the LC50 of the extracts was higher than 1000 μL/mL.     

月季花抗氧化物的提取及其对猪油抗氧化作用的研究

以猪油为底物 ,采用Na2 S2 O3 I2 滴定法测定了月季花提取物的抗氧化活性。结果表明 :月季花95 %乙醇粗提物对猪油的抗氧化作用最强 ,其抗氧化作用随提取物浓度增加而逐渐增强。柠檬酸、半胱氨酸等对月季花提取物具有协同抗氧化作用。特别是抗坏血酸、维生素E与提取物复配 ,具有显著的协同抗氧化作用 ,复合物的抗氧化性优于茶多酚 ,几乎可与特丁基对苯二酚相媲美