胸腹水中癌胚抗原和LDH及Glu水平的临床鉴别诊断价值

目的探讨癌胚抗原(CEA)和乳酸脱氢酶(LDH)及葡萄糖(Glu)水平在良恶性胸腹水中的临床鉴别诊断意义.方法分别采用化学发光标记免疫法、速率法和葡萄糖氧化酶法检测105例患者不同性质胸腹水中CEA和LDH及Glu的浓度,检测结果采用方差分析和Dunett'C法进行统计分析.结果恶性肿瘤组患者胸腹水标本中CEA值较结核性胸膜炎组和慢性非特异性炎症组明显升高(P＜0.05);恶性肿瘤组和结核性胸膜炎组标本LDH值之间差异无显著性(P＞O.05),但均高于慢性非特异性炎症组(P＜0.05);恶性肿瘤组和慢性非特异性炎症组标本Glu值差异无显著性(P＞0.05),但均高于结核性胸膜炎组(P＜0.05).结论联合定量检测CEA和LDH及Glu对胸腹水性质的鉴别诊断有重要临床参考价值.     

胸腹水中癌胚抗原和LDH及Glu水平的临床鉴别诊断价值

目的探讨癌胚抗原(CEA)和乳酸脱氢酶(LDH)及葡萄糖(Glu)水平在良恶性胸腹水中的临床鉴别诊断意义。方法分别采用化学发光标记免疫法、速率法和葡萄糖氧化酶法检测105例患者不同性质胸腹水中CEA和LDH及Glu的浓度,检测结果采用方差分析和Dunett’C法进行统计分析。结果恶性肿瘤组患者胸腹水标本中CEA值较结核性胸膜炎组和慢性非特异性炎症组明显升高(P<0.05);恶性肿瘤组和结核性胸膜炎组标本LDH值之间差异无显著性(P>0.05),但均高于慢性非特异性炎症组(P<0.05);恶性肿瘤组和慢性非特异性炎症组标本Glu值差异无显著性(P>0.05),但均高于结核性胸膜炎组(P<0.05)。结论联合定量检测CEA和LDH及Glu对胸腹水性质的鉴别诊断有重要临床参考价值。     

五种指标检测对胸腹水诊断价值的探讨

报告67例不同疾病患者胸腹水中乳酸脱氢酶（简称LDH），腺苷酸脱氢酶（Adenosie deaminae ADA),纤维蛋白原隆解产物（简FDP），铁结合蛋白（ironbinding protein IBP)和C－反应性蛋白（C－redctition protein CRP) 五项指标结果观察，其浓度依次产核性胸膜炎＞炎症＞恶性肿瘤＞肝癌＞肝硬化，肝炎，前三者之间（除ADA外），后两者之间五项检测均值差异无显著意义（P＞0．05），而后两者与前三者之间差异有显著意义（P＜0．01），恶性肿瘤与前两者ADA含蛳有显著意义（P＜0．01），炎症CRP检测率明显高于结核性胸膜炎和其它，认为联合检测弥补了单项测定的不足，提高了鉴别诊断。     

脑脊液乳酸检测在小儿脑膜炎中的诊断意义

目的探讨脑脊液乳酸检测在小儿脑膜炎临床鉴别诊断中的意义。方法采用酶显色法检测125例患儿的脑脊液乳酸（CSF-LA）水平。结果化脓性脑膜炎组和结核性脑膜炎组CSF-LA水平明显高于病毒性脑膜炎组和对照组,差异均有统计学意义（P〈0．001）;化脓性脑膜炎组CSF-LA水平高于结核性脑膜炎组（P〈0．05）,且经正规治疗1周后,化脓性脑膜炎组患儿CSF-LA水平与治疗前相比明显下降（P〈0．001）,而结核性脑膜炎组患儿其CSF-LA水平与治疗前比较无明显变化（P〉0．05）;病毒性脑膜炎组CSF-LA水平与对照组相比,差异无统计学意义（P〉0．05）。结论CSF-LA水平检测对小儿脑膜炎的鉴别有一定的诊断价值,特别是动态观察CSF-LA水平的变化对小儿化脓性脑膜炎和结核性脑膜炎的鉴别诊断具有更重要的参考价值。     

胸腔积液病人ADA LDH CEA TB—Ab联合检测的临床价值

目的探讨胸腔积液病人腺苷脱氨酶（ADA）、乳酸脱氢酶（LDH）、癌胚抗原（CEA）、结核抗体（TB—Ab）联合检测在胸水定性中的价值。方法对胸腔积液病人311例（结核胸膜炎组168例，恶性胸水组98例，炎性胸水组45例）进行ADA、LDH、CEA、TB—Ab的测定。结果三组ADA测定值均有显著性差异（P〈0．01）。恶性组与结核组、炎性组CEA测定值有显著性差异（P〈0．01），结核胸水组与炎性胸水组CEA测定值无差异（P〉0．05）。结核胸水组与恶性胸水组LDH测定值无明显差异（P〉0．05）；两组与炎性胸水痘组LDH测定值有差异（P〈0．01）。结核胸水组与恶性胸水组及炎性胸水组结核抗体均有显著差异（P〈0．01），恶性胸水组与炎性胸水组无明显差异（P〉0．05）。结论ADA、TB—Ab、CEA联合测定对胸腔积液定性很有意义，LDH测定对胸水定性意义不大。     

重症颅脑外伤急性期联合检测空腹血糖和糖化血红蛋白及乳酸脱氢酶临床价值

目的探讨联合检测空腹血糖（fasting plasma glucose,FPG）、糖化血红蛋白（glycosylated hemoglobin A1C,HbA1c）及乳酸脱氢酶（1actate dehydrogenass,LDH）在重症颅脑外伤急性期患者病情评估及预后判断中的价值。方法52例重症颅脑损伤患者,分别依据人院时格拉斯哥昏迷评分（GlasgowComaScale,GCS）、FPG联合HbAtc检测情况及GCS预后评分进行分组,比较重度颅脑损伤组（GCS评分6～8分）与极重度颅脑损伤组（GCS评分3～5分）、糖尿病高血糖组（FPG〉6．1mmol／L且HbAlc〉6．2％）与应激性高血糖组（FPG〉6．1mmol／L且HbAlc〈6．2％）及不同预后患者LDH、FPG水平,并分析GCS评分与LDH、FPG的相关性。结果极重度颅脑损伤组FPG、LDH水平高于重度颅脑损伤组（P〈O．01）;糖尿病高血糖组与应激性高血糖组FPG、LDH水平及预后比较差异无统计学意义（P〉0．05）;恢复良好组、预后差组、死亡组LDH、FPG水平比较差异均有统计学意义（P〈0．01）;重症颅脑外伤患者人院时GCS评分与LDH、FPG水平呈负相关（r=-0．948,P=0．001;r=-0．941,P=0．001）。结论FPG、LDH可用于评估颅脑损伤严重程度,判断患者预后,HbA,c在鉴别诊断糖尿病性或应激性血糖增高中有重要价值。     

血清及胸水LDH、ADA水平检测对结核性胸膜炎患者诊断准确率的影响

目的:研究血清及胸水中腺苷脱氨酶(ADA)、乳酸脱氢酶(LDH)水平检测在结核性胸膜炎患者中的诊断效果。方法:选取2017年4月~2017年10月我院结核性胸膜炎患者61例作为观察组,同时选取肝性胸水患者40例作为对照组。检测两组血清及胸水中ADA、LDH水平,对比血清及胸水中ADA、LDH水平单独与联合诊断灵敏度、准确度。结果:观察组血清及胸水中ADA、LDH水平高于对照组,差异有统计学意义(P 0. 05);联合诊断灵敏度(96. 72%)、准确率(89. 11%)高于血清及胸水中ADA、LDH水平单独诊断,差异有统计学意义(P 0. 05)。结论:血清及胸水中ADA、LDH水平联合诊断结核性胸膜炎,能有效提高诊断灵敏度、准确度,为临床鉴别提供重要参考依据,值得推广应用。     

中枢神经系统白血病患者脑脊液胱抑素C、β2-微球蛋白及乳酸脱氢酶水平观察

目的探讨脑脊液胱抑素C、β2-微球蛋白（β2一MG）及乳酸脱氢酶（LDH）在中枢神经系统白血痛（CNSL）诊断中的价值。方法对15例伴有CNSL的急性白血病（AL）患者,21例不伴CNSL的AL患者和12例排除中枢神经系统疾病患者（对照组）进行脑脊液胱抑素C、β2一MG及LDH水平的测定,并进行比较。结果AL伴有CNSL组的脑脊液胱抑素C、β2一MG及LDH水平与AL不伴CNSL组、对照组比较差异均有统计学意义（P〈0．01）;AL不伴CNSL组的脑脊液胱抑素C、β2-MG及LDH水平与对照组比较,差异无统计学意义（P〉0．05）。CNSL发生前的脑脊液胱抑素C水平与发生中相比差异无统计学意义（P〉0．05）;CNSL发生前的脑脊液p。一MG和LDH水平与发生中相比有显著性差异（P〈0．01）;CNSL缓解后的脑脊液胱抑素C、β2一MG及LDH水平与发生中相比有显著性差异（P〈0．01）。结论脑脊液胱抑素C、β2-MG及LDH水平检测是CNSL诊断和疗效判断的有效指标,持续性脑脊液胱抑素C低水平可能增加CNSL发生的危险性。     

血小板参数联合乳酸脱氢酶在血小板增多症鉴别诊断中的价值

本研究探讨血小板参数联合乳酸脱氢酶在血小板增多症鉴别诊断中的价值。常规检测骨髓增殖性疾病组（CMPD组）[包括慢性髓系白血病（CML）、真性红细胞增多症（PV）、原发性血小板增多症（ET）]和反应性血小板增多症组（RT组）血小板计数（Plt）、平均血小板体积（MPV）、血小板体积分布宽度（PDW）、大血小板比率（P—LCR）和血清乳酸脱氢酶酶（LDH）水平,应用非参数检验进行统计学分析,绘制ROC曲线,判断各个指标在血小板增多症患者鉴别诊断中的价值,并确定最佳截断值。结果表明,CMPD组血小板参数MPV、PDW、P-LCR和LDH各项指标均明显高于RT组（P〈0．05）,可以鉴别CMPD和RT,尤以PDW、P—LCR预测CMPD的诊断效能最高,ROC曲线下面积分别为0．96、0．89,最佳截断值分别为11．95％、23．05％;鉴别3种CMPD的方法如下：如LDH、P—LCR明显升高,应考虑CML,其最佳预测截断值分别为424U／L、26．10％;如LDH中度升高,同时Pit明显升高,应考虑ET,Plt最佳预测截断值为939×10^9／L。结论：血小板参数联合LDH有助于血小板增多症的鉴别诊断．它是一种简单、快速、初步的鉴别方法,尤其适合基层医院推广应用。     

血清APRIL表达水平与非霍奇金氏病相关性的初步研究

目的研究非霍奇金氏病（NHL）患者血清增殖诱导配体（APRIL）蛋白水平，并探讨其与血清乳酸脱氢酶（LDH）的关系。方法应用酶联免疫吸附试验（ELISA）法检测LDH升高患者组（10例）和LDH正常NHL患者组（12例）血清APRIL水平，以健康志愿者（23例）作为对照；同时将血清APRIL水平与患者血清乳酸脱氢酶（LDH）进行相关性分析。结果NHL患者血清APRIL水平与健康对照组无显著性差异（P〉0．05），但LDH升高患者组血清APRIL水平明显高于LDH正常患者组（P〈0．05）；NHL患者组和LDH升高患者组血清APRIL水平与LDH正相关（r=0．923,0．799，P〈0．05）。结论NHL患者血清APRIL水平与健康对照组无显著性差异，LDH升高患者组血清APRIL水平明显高于LDH正常患者组，提示APRIL可能与NHL的预后及恶性程度有关。     

LDH isozymes of human T and B lymphocytes

Peripheral lymphocytes were obtained from five normal healthy adults. T and B lymphocytes were separated by rosette formation. LDH (L-lactate:NAD⁺ oxidoreductase, EC 1.1.1.27) isozymes were studied on both populations after agar gel electrophoresis.

The mean B lymphocyte pattern was: LDH-1, 7.2%; LDH-2, 25.0%; LDH-3, 39.2%; LDH-4, 21.9%; LDH-5, 6.6%.

The mean T lymphocyte pattern was: LDH-1, 25.8%; LDH-2, 35.1%; LDH-3, 28.3%; LDH-4, 9.4%, LDH-5,1.3%.

This makes the T lymphocytes look more aerobic than the B lymphocytes.

Daudicells had the following pattern: LDH-1, 8.4%; LDH-2, 19.1%, LDH-3, 26.6%, LDH-4, 24.9%; LDH-5, 20.9%.     

牵引推拿并电针理疗治疗LDH疗效观察

目的 观察牵引推拿并电针理疗治疗腰椎间盘突出症(以下简称LDH)的治疗效果,探讨LDH中西医保守疗法的综合运用模式.方法 采用牵引推拿和电针理疗综合方法 ,对50例LDH患者进行治疗观察,并以单纯牵引推拿组及电针理疗组各50例作对照,治疗结束时评定疗效,统计数据经Ridit分析法处理.结果 综合治疗组治愈显效率86%,总有效率98%;牵引推拿组分别为68%、94%;电针理疗组分别为62%、90%.综合治疗组明显优于两对照组(χ2=-14.93,P<0.01).结论 牵引推拿并电针理疗综合运用治疗LDH能显著提高治疗效果,具有较高的临床应用价值.     

Interference of the measurement of lactate dehydrogenase (LDH) activity in human serum and plasma by LDH from blood cells

This study was performed to find a reliable method to measure lactate dehydrogenase (LDH) activity in blood samples. Human plasma contains thrombocytes whose number depends on the speed of centrifugation applied to the freshly-drawn blood sample. They do not disturb the measurement of LDH in plasma, provided the plasma sample has not been subjected to procedures that may destroy the integrity of the thrombocytes, e.g. standing overnight at 4 degrees C, freezing, or treatment with ultrasonic waves. Centrifugation of plasma at a minimum of 1174 x g eliminates the presence of thrombocytes. The serum of healthy human subjects invariably contains higher LDH activity than the plasma: the difference is 30 +/- 18 U/l (mean +/- S.D.). LDH-isoenzyme analysis shows that a large part of the LDH activity difference is caused by lysis of erythrocytes in the clot. Liberation of 30 U LDH per litre is not associated with visible hemolysis.     

Elevated level of serum LDH2 and LDH3 in sputum three positive TB patients of Sahariya tribe: a preliminary study

OBJECTIVES: The objective of this investigation was to find out if sputum-positive (AFB test) test, which is performed to assess mycobacterial infection status, is anyway correlated with any of the LDH isoforms. And if so, can it be used, either alone or together with sputum test, as a rapid on-the-spot marker for field diagnosis of tuberculosis. DESIGN AND METHODS: To analyze the relationship between sputum test results and the level of LDH isozyme (isoforms), 157 individuals were randomly selected from a Sahariya tribal population, with a known history of tuberculosis, for sputum and blood collection. The Ziehl-Neelsen's staining of sputum smear was done as per RNTCP (Revised National Tuberculosis Control Programme) protocol. In all the samples, serum LDH level was estimated spectrophotometrically while the levels of individual isoforms were assessed on native PAGE. RESULTS: The LDH content was significantly higher in blood sera of sputum-positive (three positive) individuals (444+/-270 IU) as compared to sputum-negative samples (242+/-125 IU). Analysis on the association of different LDH isoforms (LDH1, 2, 3, 4 and 5) with sputum test revealed significantly higher frequency of LDH2 and LDH3 in sputum-positive samples as compared to sputum-negative samples. In sputum three positive cases, however, the frequency of LDH3 appeared much higher (in 60% cases) than LDH2 (in 33% cases), which was found to be almost same in sputum negative but higher in two positive samples (44.4%). CONCLUSIONS: The present data suggests a strong association of LDH3 with sputum three positive or severe cases of mycobacterial infection, indicating a likely possibility of using LDH3 as a supporting diagnostic marker in, at least, cases of chronic tuberculosis.     

乳酸脱氢酶及其同工酶在胸腔积液中的临床意义

目的 探讨乳酸脱氢酶（ＬＤＨ）及其同工酶对鉴别诊断胸腔积液中良、恶性疾病及监测其病程预后的临床价值。方法 将２０７例伴有胸水的肺癌、结核性胸膜炎及肺炎、心功能不全、心力衰竭患者分为三组，ＬＤＨ采用酶法检测，其同工酶则以醋纤琼脂糖电泳法进行酶谱分析。分别检测患者胸水和血清ＬＤＨ及其同工酶。结果 结核性胸膜炎患者胸水ＬＤＨ增加显著，为１２９４±２１２．９Ｕ／Ｌ与自身血清相比有显著差异（Ｐ〈０．００１）