PRENATAL DIAGNOSIS Ⅱ. AMNIOTIC FLUID ACETYLCHOLINESTERASE ISOENZYME PATTERNS AND ALPHA-FETOPROTEIN FOR PRENATAL DIAGNOSIS

Both amniotic fluid (AF) alphafetoprotein (AFP) and acetylcholinesterase isoenzyme patterns (AChEI) were used for prenatal diagnosis. 721 samples were analyzed. The results obtained from AChEI analysis by gel electrophoresis of AF were much more accurate than those from AFP. In 58 pregnancies with uncertain gestational age, assessment became difficult because its values decreased significantly after the 16th week of gestation but AChEI was not influenced by gestational ages. Both methods are reliable in the diagnosis of open neural tube defects (NTDs) in early pregnancy.     

FIELD STUDIES IN THE DIAGNOSIS OF SCHISTOSOMIASIS BY MONOCLONAL ANTIBODYDOT-ELISA

ＦＩＥＬＤＳＴＵＤＩＥＳＩＮＴＨＥＤＩＡＧＮＯＳＩＳＯＦＳＣＨＩＳＴＯＳＯＭＩＡＳＩＳＢＹＭＯＮＯＣＬＯＮＡＬＡＮＴＩＢＯＤＹＤＯＴ－ＥＬＩＳＡＬｏｎＷｅｄａｎ（娄文娴），ＺｈｕＸｕｍｉｎｇ（朱旭明），ＹａｎｇＳｈｉｊｉｎｇ（杨士静）．Ｄｅｐａｒｔｍ...     

AUTOFLUORESCENCE EXCITED BY ENDOSCOPIC XENON ION LASER IN THE DIAGNOSIS OF GASTRIC CANCER

Endoscopic xenon ion laser induced autofluorescence spectra in various gastric diseases were tested in 104 patients diagnosed by endoscopy and biopsy, including 28 gastric cancer, 9 gastric ulcer, 46 chronic atrophic gastritis, and 21 superficial gastritis. The autofluorescence spectrum excited with the 365 nm line of pulsed xenon ion laser was measured. The xenon ion laser beam having an output energy of 0.5 mJ with a duration of 0.6μs and a repetition rate of 10 Hz was used as the excitation source. A coaxial optical cable for laser light and autofluorescent light transmission was inserted via the biopsy channel of an endoscope into the cavity of stomach. The tip of opitcal cable was aimed at the gastric tissue and autofluorescence spectrum was plotted on X-Y recorder. As the monochromator scanned in the range 500-750 nm, a single-pulse fluorescence spectrum could be obtained by using an optical multichannel analyser, and then this fluorescence spectrum was stored in the computer. The whole procedure could be completed within 15 seconds. The results of the present study show that autofluorescence spectra of gastric cancer have characteristic peaks near 630 nm and 690 nm, while no such peaks appear in the spectra of the corresponding normal regions. A consistency of 75% was observed in gastric cancer as compared with the pathologic diagnosis. It is claimed that this is a harmless, safe, simple, and fast procedure in the diagnosis of gastric cancer.     

CLINICAL EXPERIENCE OF DR. SHAO JINGMING IN TREATMENT OF DISEASES BY PUNCTURING BACK-SHU POINTS

Dr.Shao Jingming has been engaged inclinical acupuncture teaching for more than60 years and accumulated rich experience.He has always put stresses on differentiationof diseases according to pathologicalchanges of the viscera and theirinterrelations,and proposed utilization ofthe Back—Shu points in treatment of dis-eases.He is good at treating diseases by se-lecting.few but highly effective acupoints. Inthis paper the authors summarize the expe-rience of Dr.Shao in the clinical applicationof Back—Shu points,and would like to share     

MORPHOMETRIC STUDY OF GASTRIC PRECANCEROUS LESIONS: I. QUANTITATIVE DIAGNOSIS OF CELL ATYPISM IN GASTRIC BIOPSY SPECIMENS

The cell atypism is one of the important criteria for making diagnosis of precancerous lesions of gastric mucosa, in routine pathological examination, it is evaluated by the degree of atypism of cell nucleus. 5 461 cells in 50 foci of gastric mucosa with intestinal type of lesions (including simple intestinal metaplasia 10; intestinal type dysplasia in different grades: mild 10. moderate 11, severe 9; intestinal type gastric carcinoma 10) have been submitted to morphometric analysis by automatic image analysis system (Quantimet 900). 7 geometric parameters of nucleus were measured by analysis, including: (1) Area; (2) Perimeter; (3) Diameter (2. Area/π1/2; (4) Length; (5) Breadth; (6) Axis ratio (Length / Breadth); (7) Contour (Perimeter~2 / 4π·Area), these parameters represent the size and shape of nucleus. The results of measurements indicated that value of the above mentioned parameters, except the axis ratio and the contour of cell nucleus, all increased in accord with the increase of severity of the atypism in various intestinal type lesions, hence they can be used as objective criteria for quantitative evaluation of gastric precancerous lesions.     

A CLINICAL STUDY ON TERMINATION OF EARLY PREGNANCY BY DL-15-METHYL PROSTAGLANDIN F_(2α) METHYL ESTER IN COMBINATION WITH TESTOSTERONE PROPIONATE OR MESTRINONE

The present investigation was to study the abortifacient effect of a single vaginal suppository containing old or new craft~* dl-15-methyl PGF_(2α) methyl ester in combination with testosterone propionate or mestrinone (R_(2323)). A total of 360 early pregnant women were included in this study. The results showed that the complete abortion rate in early pregnancy termination with dl-15-methyl PGF_(2α) methyl ester in combination with testosterone propionate or R_(2323) was 86.19% and 84.8% respectively. There was no significant difference between two combinations (P>0.05). And the early pregnancy termination with old craft dl-15-methyl PGF_(2α) methyl ester in combination with above mentioned two drugs and new craft PG with the two drugs was 85.02% and 85.92% respectively (P>0.05). The PG of two crafts has the same efficacy but the new one is cheaper and therefore more preferable. There was a temporal liver function alteration in 19.3% of the women who took R_(2323). We consider dl-15-methyl PGF_(2α) methyl ester vaginal suppository in combination with testosterone a more favorable formulation for termination of early pregnancy.     

DIFFERENT EFFECTS OF REINFORCING AND REDUCING MANIPULATIONS IN ACUPUNCTURE ASSESSED BY TAIL FLICK LATENCY,VOCALIZATION THRESH.OLD AND SKIN TEMPERATURE IN THE RAT

In this paper,different effects of acupunctureat the Changqiang (Du 1) point with manipulationsof slow and rapid needlings for reinforcing and re-ducing were assessed by tail flick latency,vocaliza-