平行平板流动装置用于药物对内皮细胞功能影响的实验研究

目的：将平行平板流动装置用于体外药物试验研究，为心血管药物的筛选和测试提供新的实验方法和手段。方法用放射免疫法测定内皮素（ＥＴ－１）和６－酮－前列腺腺素Ｆ１α（６－keto-PGF1α)的分泌速率累积分泌量，观察复方丹参注射液、丹皮酚和阿司匹林等心血管药物在剪切流场中对内皮细胞分泌ＥＴ－１和前列腺素（ＰＧＩ２）功能的干预作用。结果复方丹参注射液和丹皮酚对ＥＴ－１、ＰＧＩ２的分泌功能均促进作用，而阿司匹林则无影响。结平行平板流动装置条件易控制，观察和测定方便，不失为一种研究心血管药物对内皮细胞功能影响的较好方法，实验结果有利于对丹参和丹皮酚的血管作用有效的认识。     

紫外线照射自血回输治疗非器质性头痛26例临床分析

观察了26例病程长,经药物治疗无效的功能性头痛患者,用紫外线照射自血回输疗法(UBI)后取得了满意的疗效(有效率100％;优良率56％),分析其原因系由于UBI后改变了血液流变性,使血粘度下降,纤维蛋白原降低,血小板的粘附性,聚集性降低,红细胞的变形能力增强,从而增加了血液的流速,使组织的供血供氧改善,解除了血管痉挛,从而缓解了头痛。我们认为,对功能性头痛,UBI是值得推广的治疗方法。     

丹皮酚和阿司匹林对人结肠癌LoVo细胞增殖的影响及其机制

目的:探讨丹皮酚和阿司匹林和阿司匹林对人结肠癌细胞系LoVo细胞增殖的影响及可能作用机制。方法:用不同浓度丹皮酚(15.63-250mg/L)和阿司匹林(98.08-1 801.6mg/L)处理体外培养的LoVo细胞24,48,72,96h,CKK-8比色法测定其对结肠癌LoVo细胞的活力的影响;根据CKK-8结果分为丹皮酚组(浓度31.25,62.50,125mg/L)和阿司匹林组(浓度分别为180.16,900.80,1 801.6mg/L),进一步处理48h后行流式细胞仪检测细胞凋亡、激光共聚焦显微镜检测细胞内Ca2+浓度的变化及RT-PCR法检测RUNX3基因表达情况。结果:丹皮酚(浓度15.63-250mg/L)和阿司匹林(浓度90.08-1 801.6mg/L)均能能显著降低LoVo细胞存活率,呈剂量、时间依赖性;丹皮酚组处理细胞48h后可诱导细胞凋亡,流式细胞仪检测凋亡率分别为13.5%,21.4%,34.6%,明显高于对照组;阿司匹林组细胞凋亡率分别为14.8%,25.8%,37.7%,明显高于对照组;丹皮酚组荧光强度分别为41.36±4.62,57.51±3.83和69.43±3.76;阿司匹林组荧光强度分别为38.24±4.62,53.31±4.92和65.64±5.25,丹皮酚组、阿司匹林组与对照组(荧光强度24.45±3.74)比较,差异均有统计学意义(P<0.05);丹皮酚和阿司匹林均能上调RUNX3mRNA的表达,呈剂量依赖性。结论:丹皮酚、阿司匹林均能抑制LoVo细胞的增殖并诱导其凋亡,其作用机制可能与增加细胞内Ca2+含量和上调RUNX3基因的表达有关。丹皮酚与阿司匹林可能具有相似抗大肠癌细胞作用机制。     

美国血液流变学研究近况——红细胞变形性研究

美国圣路易斯市华盛顿大学生物医学工程系、纽约哥伦比亚大学医学院与旧金山加利福尼亚大学医学院是美国研究血液流变学的三个主要基地,但研究重点有所不同。华盛顿大学的主要研究课题是各种疾病及药物对红细胞变形性的影响,哥伦比亚大学的主要研究领域是血液及心血管疾病时血液流变性的改变,加利福尼亚大学的研究重点是血液流变性与肿瘤的关系。本文仅介绍华盛顿大学的血液流变学研     

芪丹通脉片对高脂血症大鼠血液、细胞流变性及红细胞膜流动性的影响

目的：观察中药复方制剂芪丹通脉片（QDTMT）对高脂血症SD大鼠血液流变性,细胞流变性及红细胞膜流动性的影响。方法：复制高脂血症大鼠模型,同时用QDTMT灌胃。于第16d从眼眶静脉丛取血,测定血脂、血液流变性、细胞流变性及红细胞膜流动性。结果：QDTMT能降低实验性高脂血症大鼠全血黏度、血浆黏度、纤维蛋白原及红细胞聚集指数（AI）,提高红细胞变形能力（DI）,能够降低荧光偏振度,减少红细胞丙二醛（MDA）的含量。结论：QDTMT能明显改善高脂血症大鼠血脂紊乱、血液流变性,细胞流变性,红细胞膜流动性。     

冠心二号方对血液流变学性影响

本文探讨了冠心二号组成药对血液流变性参数的影响，结果显示，川芎，丹参，红花，降香对红细胞流变性均有不同程度的改善作用。可显著降低红细胞相互吸引力，增加红细胞表面电荷，从而降低红细胞聚集性。红花，赤芍可改善血栓弹力图参数。川芎，丹参，赤芍可显著改善血小板粘附性，川芎，赤芍和降香可纤维蛋白元，所有药物均显示降低血小板聚集性作用。     

福宝片抗氧化、血流变、心肌保护作用的实验研究

研究表明福宝片具显著的抗氧化作用,降低大鼠血浆MDA含量,提高SOD活性;改善大鼠血液流变性,使全血粘度降低,红细胞变形能力增强;并对心肌缺血有明显的保护作用。     

宫环止血片对置铜宫内节育器家兔血液流变学的影响

目的：研究宫环止血片对置铜宫内节育器（Cu-IUD)所致子宫异常出血的影响，方法：用吲哚美辛作对照，观察了宫环止血片对置Cu-IUD家兔血液中血沉，红细胞比容，全血比粘度（低，中，高切），血浆比粘度等血液流变学指标的影响，结果：宫环止血片能降低红细胞比容，降低全血比粘度和血浆比粘度，从而改变血液流变性，达到祛瘀止血的目的。结论：提示改变血液流变性可能是宫环止血片防治置Cu-IUD引起的出血的机理之一。     

脾虚与肝郁证患者血液流变学的变化特点

本文检测了60例脾虚和肝郁证病人的血液流变性.结果表明脾虚病人表现为低粘状态和出血倾向.即红细胞压积、血液粘度、血浆粘度及纤维蛋白原的显著降低和红细胞硬化性及聚集性的增高.与肝郁证组及正常组比较,P<0.05或P<0.01.而肝郁证则表现为血液还原粘度与血浆粘度的升高.提示二证俱存在血液瘀滞现象,但血液循环基础不同。这对指导临床治疗有一定意义。同时本文观察到病种和病情对血液流变性也有一定影响。     

闭塞性动脉硬化156例血液流变学观察

采用血液流变学检测方法,对156例闭塞性动脉硬化症患者进行检测、临床观察。数据记录分析后发现:①患者血液流变学特征为血浆粘度、纤维蛋白原、红细胞沉降率、全血比高切牯度超过正常人组(P<0.001);红细胞压积无明显改变(P>0.05)。②血液流变性改变与临床病情变化呈正相关。③血液流变性改变与中医辨证分型存在密切关系。④中西医结合疗法可改善本病患者血液流变性异常。     

前列腺素E1治疗脑梗塞临床血液流变学观察

作者将40例经临床及CT扫描证实的急性脑梗塞患者随机分为前列腺素E_1(PGE_1)治疗组和低分子右旋糖酐对照组进行疗效观察,并对治疗前后血液流变学指标进行了分析.结果表明,PGE_1组总有效率95%,痊愈及显效率80%,痊愈及显效率较对照组疗效显著(P<0.05).PGE_1组用药后全血比粘度、血小板粘附率、血脂下降明显(P<0.05).提示PGE_1具有降粘、抗凝、解聚、溶栓作用.     

莪术活血化瘀有效物质研究

通过体外血小板聚集、大鼠血瘀模型血液流变性及凝血试验。观察莪术不同提取物的活血化瘀作用。采用血小板聚集功能测定法、血液流变性测定法及小鼠抗凝法观察莪术不同组分活血化瘀作用。结果显示：莪术不同提取物均具一定的抗血小板聚集、抗凝血及调节血液流变性作用。其中以乙酸乙酯、氯仿提取物活性最强。莪术中姜黄素类成分为其活血化瘀主要活性成分。本实验为阐明莪术有效物质提供了科学依据。     

参芍颗粒对犬血液流变学的影响

目的：观察参芍颗粒对结扎犬冠状动脉前降支造成血瘀症模型的血液流变学的影响。方法：采用结扎犬冠状动脉前降支造成血瘀症模型,于造模前、造模后15,30,60,90min分别股静脉采血测定血液流变学指标、体外血栓长度、血小板粘附率和全血粘度（切变率为5－200／s)。结果：参芍颗粒可缩短血栓长度、降低全血粘度、血小板粘附度,抑帛血栓形成。结论：参芍颗粒有改善实验性犬血瘀症的血液流变学指标的作用。     

Comparative Study on the Effects of Small Dose of Aspirin Combined with Ginkgo-damole Injection(银杏达莫注射液) and the Effects of Conventional Dose of Aspirin in Treating Senile Unstable Angina Pectoris

Objective: To evaluate the therapeutic and adverse effect of small dose of aspirin (Asp) combined with Ginkgo-damole injection (GDI,银杏达莫注射液) in treating senile unstable angina pectoris (UA). Methods: One hundred and twenty old in-patients of coronary heart disease with UA were randomly divided into two groups. The 60 patients in the treated group were treated with oral administration of enteric soluble 50 mg Asp once a day and GDI 20 ml per day by intravenous dripping, with 14 days as one therapeutic course, while the other 60 in the control group were treated with enteric soluble Asp 100 mg alone once a day orally. Besides, isosorbide-5-mononitrate 20 mg twice a day was applied to both groups, and the β-ad-renoceptor blocker, blood lipids regulatory agents and nitroglycerin (10 mg by intravenous dripping) were given accordingly. The angina total improving rate, hemorrheologic indexes (whole blood viscosity, plasma viscosity, fibrinogen, platelet aggregation rate), comprehensive clinical terminal     

Comparative study on the effects of small dose of aspirin combined with Ginkgo-damole injection and the effects of conventional dose of aspirin in treating senile unstable angina pectoris

Objective: To evaluate the therapeutic and adverse effect of small dose of aspirin (Asp) combined with Ginkgo-damole injection (GDI) in treating senile unstable angina pectoris (UA).Methods: One hundred and twenty old in-patients of coronary heart disease with UA were randomly divided into two groups. The 60 patients in the treated group were treated with oral administration of enteric soluble 50 mg Asp once a day and GDI 20 ml per day by intravenous dripping, with 14 days as one therapeutic course, while the other 60 in the control group were treated with enteric soluble Asp 100 mg alone once a day orally. Besides, isosorbide-5-mononitrate 20 mg twice a day was applied to both groups, and the β-adrenoceptor blocker, blood lipids regulatory agents and nitroglycerin (10 mg by intravenous dripping) were given accordingly. The angina total improving rate, hemorrheologic indexes (whole blood viscosity, plasma viscosity, fibrinogen, platelet aggregation rate), comprehensive clinical terminal event and the total occurrence rate of adverse reaction in the two groups were observed.Results: After treatment, comparison between the two groups showed insignificant difference in aspects of angina total improving rate (75.00% vs 65.00%), hemorrheological indexes and comprehensive clinical terminal event rate (25.00% vs 31.67%),P>0.05, and the hemorrheological indexes were improved in both groups (P<0.05), but the total occurrence rate of adverse reaction in the treated group was lower than that in the control group (6.67% vs 25.00%), showing significant difference (P<0.05).Conclusion: In treatment of senile UA, small dose of Asp combined with GDI showed therapeutic effect similar to that of conventional dose of Asp, but it has lower adverse reaction.     

Effect of Xuesaitong soft capsule on hemorrheology and in auxiliarily treating patients with acute cerebral infarction

Objective: To observe the therapeutic effect of Xuesaitong soft capsule (XST and its effect on platelet counts, coagulation factor 1 (CF1) as well as hemorrheologic indexes in treating patients with acute cerebral infarction (ACI).Methods: Two hundred and four patients with ACI were assigned into two groups, the control group (n = 96) and the treated group (n = 108). They were all treated with conventional Western medicines, including mannitol, troxerutin, citicoline, piracetam and aspirin, while to the treated group, XST was given additionally through oral intake, twice a day, 2 capsules each time for 8 successive weeks. The clinical efficacy was evaluated according to the nerve function deficits scoring and the changes of platelet count. CF1 and hemorrheological indexes were measured before and after treatment.Results: The total effective rate was 87.0% in the treated group, and 87. 5% in the control group, respectively, showing insignificant difference between them. But the markedly effective rate in the treated group (66.7%) was significantly higher than that in the control group (27.1%,P<0. 01). The count of platelet was not changed significantly in both groups after treatment, while CF1 in them evidently lowered at the end of the 4th and 8th weeks of treatment, but showed insignificant difference between the two groups. The hematocrit, whole blood viscosity and plasma viscosity in both groups were all improved significantly after treatment, but also showed insignificant difference in comparison of the two groups.Conclusion: XST has good efficacy in auxiliary treatment of patients with ACI, though its mechanism remains to be further explored.     

痛风急性发作期中医辨证分型与血流变及血小板活化功能关系的研究

目的 探讨痛风急性发作期患者的中医辨证分型与血液流变学、血小板活化功能的关系.方法 选择痛风急性发作期患者40例,健康体检者20例为对照组,以辨证分型、血液流变学、血小板活化功能PAC-1,CD62p检测为指标.结果 痛风急性期患者湿热痹阻,瘀热内郁组血流变表现为全血粘度(低切、中切、高切)、血浆粘度、红细胞电泳与聚集指数均显著高于正常对照组(P＜0.01).两组血小板活化功能PAC-1,CD62p,均显著高于对照组(P＜0.01).而瘀热内郁组对比湿热痹阻组血小板活化功能PAC-1,CD62p,显著升高(P＜0.01).结论 说明痛风急性发作期中医辨证分型与血流变及血小板活化功能之间存在着相关性,湿热痹阻、瘀热内郁是痛风急性期的基本征候和原发病机,两组间指标水平的差异可能是痛风出现不同证型的内在因素之一.     

阿司匹林和氯吡格雷对大鼠血小板聚集的影响

目的：比较氯吡格雷和阿司匹林对血小板抑制作用的差异。方法：40只雄性Wistar大鼠随机分为4组,每组10只：A空白,B阿司匹林,C氯吡格雷,D体外。A、D组按5 ml/（kg.d）灌胃蒸馏水,B组按100 mg/（kg.d）灌胃给阿司匹林,C组按20 mg/（kg.d）灌胃给氯吡格雷,连续给药3 d。以胶原为诱导剂分别测定体内、体外血小板最大聚集率。结果：A,B,C组体内血小板最大聚集率分别为58.7%±4.8%,46.4%±3.1%和39.8%±3.9%,B和C组与A组比较差异有显著性。B和C组血小板抑制率分别为20.9%和32.2%。空白组、阿司匹林组和氯吡格雷组体外测试血小板最大聚集率分别为53.18%±6.45%,13.19%±6.92%和20.92%±9.57%,阿司匹林组和氯吡格雷组与空白组比较差异有显著性。阿司匹林组和氯吡格雷组血小板抑制率分别为75.20%和61.66%。结论：以胶原为诱导剂,与空白组比较阿司匹林与氯吡格雷在体内外对血小板聚集均有抑制作用。     

阿司匹林抑制急性心肌梗死患者血小板与白细胞的粘附

目的　研究阿司匹林（乙酰水杨酸,ＡＳＡ）对急性心肌梗死（ＡＭＩ）患者血小板与白细胞粘附的影响及其作用机制。方法　采用体外玫瑰花结形成实验检测血小板与白细胞粘附,观察ＡＳＡ 对两者粘附的作用;用１２５Ｉ标记的单克隆抗体测定血小板表面α颗粒膜蛋白１４０（ＧＭＰ１４０）的表达,观察ＡＳＡ对凝血酶诱导的血小板表面ＧＭＰ１４０ 表达的影响。结果　ＡＭＩ患者血小板与白细胞间粘附率增高,凝血酶通过诱导血小板表面ＧＭＰ１４０ 的表达促进血小板与白细胞粘附;阿司匹林（５００、５ ０００μｇ／ｍ ｌ）抑制ＡＭＩ患者血小板与白细胞间的粘附,抑制凝血酶诱导的正常人血小板与白细胞间的粘附以及凝血酶诱导的血小板表面ＧＭＰ１４０ 的表达。结论　ＡＳＡ一方面通过抑制ＧＭＰ１４０ 的表达,另一方面可能通过影响配体间的结合抑制血小板与白细胞之间的粘附,揭示了ＡＳＡ 抗栓作用的另外一种机制     

脑梗死复发患者血小板活化及阿司匹林抵抗研究

目的探讨在脑梗死复发的治疗中血小板向细胞聚集体（PLA）、血小板聚集和阿司匹林抵抗（AR）的重要作用。方法在入院当天对58例复发组脑梗死患者和116例初发组脑梗死患者采用1：2配对法,对其血小板聚集率和PLA水平进行测定。每晚开始顿服拜阿司匹林200mg,连续顿服7～10d后,通过再次测量患者的血小板聚集率,挑选出具有阿司匹林抵抗的患者。结果在入院当天的血小板聚集率测定结果和PLA水平均表现为复发组高于初发组患者,在持续顿服7-10d的拜阿司匹林后,阿司匹林抵抗发生率也表现为复发组高于初发组患者,差异均有统计学意义（P〈0．05）。结论阿司匹林抵抗和血小板活化在脑梗死复发过程中可能起着重要作用,可能是脑梗死复发的重要危险因素之一。因此在脑梗死患者的二级预防中服用阿司匹林期间有必要进行血小板聚集率的检查,尽早发现和识别AR．及时调整二级预防策略。