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1.
在3%乙二胺、1.0×10 ̄(-4)mol/L铬黑T、5;0×10 ̄(-2)mol/LKOH及0.15mol/LNaF溶液中用示波极谱法测定人发中镁,检出限为0;005mg/L线性范围为0.01mg/L~0.6mgIL,变异系数为3.7%~4.3%,样品加标回收率为95.8%~104.1%。方法简便快速,仪器设备便宜,适于基层运用。  相似文献   

2.
作者用半微分阴极吸附伏安法测定地高辛的方法,在1.0×10^-3mol/LNaOH底液中,于-1.0V富集于2min,以100mV/s向阴极扫描,有一稳定的还原峰。在地高辛浓度为4.0×10^-7mol/L-10.0×10^-7mol/L范围内,该还原峰峰高与地高辛浓度成线性,检出下限为1.0×10^-8mol/L。  相似文献   

3.
本文利用鲁米诺-氯化高铁血红素-过氧化氢发光系统,简化了流动注射流路,建立了快速、灵敏的固定化鲁米诺流动注射化学发光分析法测过氧化氢。检测的浓度范围为1.0×1O ̄(-9)~5.0×10 ̄(-4)mol/L,检出限达2.6×10 ̄(-11)mol/L。  相似文献   

4.
用溶剂萃取法提取、荧光分光光度法测定血清多巴胺含量。激发波长和发射波长分别为λex=320nm和λem=375nm,线性范围0~3.26×1O-6mol/L,回归方程Y=1.3036×10-8X+2.8224×10-8,r=0.9998,最低检出限为1.16×10-8mol/L回收率为97.75%,批内和批间CV分别为4.26%和6.52%。本法简便、快速、稳定、经济,易于批量测定,适合临床应用。  相似文献   

5.
在pH5.8乙酸-乙酸钠-铍试剂Ⅲ溶液中用示波极谱法测定人发中铝,方法简便、快速、选择性好,检出限为1.9×10-7mol/L,线性范围为4.7×10-7mol/L~1.5×10-5mol/L,变异系数为3.3%~4.3%,样品加标回收率为97.5%~104%。  相似文献   

6.
聚苯胺/聚乙烯醇复合物与四苯硼钠-尼古丁混合,制成尼古丁膜电极,在pH=2~7电极时10^-2~10^-5mol/L的尼古丁有良好的线性响应,检测下限可达4.0×10^-6mol/L。用于烟草样品中尼古丁含量的测定,平均回收率为98.4%,相对标准偏差为0.93%,与紫外分光光度法对比,结果令人满意。  相似文献   

7.
本文报道了用碳纤维微电极测定雌激素的方法,找出了最佳条件,研究了电极反应机理,并对该法进行了实际应用。线性范围达9.0×1O-8~5.0×10-6mol/L检出限5.0×10-8mol/L。  相似文献   

8.
目的:介绍、说明和应用Lee氏法测米氏常数(Km)。方法:用尿素酶与尿素反应10秒钟,用二乙酰一肟终止反应,并测定和换算出反应体系底物浓度[St]与初始底物浓度[So],代入Lee氏方程式。结果:尿素酶Km为2.9×10-3mol/L~3.1×10-3mol/L,最大反应速度V为2.2×10-4mol/S(原液)与1.2×10-4mol/S(1/2稀释液)。结论:Lee氏法测Km准确、简便,受底物消耗的影响小,反应进行了50%时,相对百分误差还不到4%。  相似文献   

9.
以四苯硼酸-汉防己甲素离子缔合物作活性材料,制成了PVC膜汉防己甲素电极。对影响电极性能的诸因素进行了讨论,以最佳条件制备的电极其线性区为5.0×10-3~5.0×10-6mol/L,平均斜率为26.5mV/pC,检测下限为1.0×10-7mol/L,在pH1.0~5.5范围内适用。电极抗干扰能力强,响应快,重现性和稳定性较好,适用于汉防己甲素制剂的快速测定。  相似文献   

10.
研究Cu~(2+)在稀氨水介质中催化H_2O_2氧化苯基荧光酮褪色反应及影响反应速率的动力学因素。据此建立催化动力学法测定痕量Cu~(2+)的新方法,其灵敏度为5.0×10~(-11)mol/L,测定范围为(0~2.5)×10~(-7)mol/L。操作简便、快速。该法可用于人体血请中痕量铜的测定。  相似文献   

11.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.  相似文献   

12.
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.  相似文献   

13.
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.  相似文献   

14.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.  相似文献   

15.
Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P<0.01);the MAP decreased(P<0.01)and the HR increased drastically(P<0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.  相似文献   

16.
Objective:To investigate the gene expression of osteoprotegerin(OPG) and osteoclast differentiation factor(ODF) in the bone tissue of patients with hip fracture due to osteoporosis. Methods:OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers(over 50 years old) with hip fracture(Observer Group) and 30 cases of hip facture sufferers with no osteoporosis(Control group) were analyzed with the Semi-Quantitative RT-PCR method. Results:The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion:Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.  相似文献   

17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.  相似文献   

18.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.  相似文献   

19.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.  相似文献   

20.
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.  相似文献   

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