Aqueous extract of Taxus Chinensis （Pilger） Rehd inhibits lung carci- noma A549 cells through the epidermal growth factor receptor/mi- togen-activated protein kinase pathway in vitro and in vivo

OBJECTIVE： To explore the anticancer mechanism of aqueous extract of Taxus Chinensis （Pilger） Rehd （AETC）. METHODS： The serum pharmacological method was used to avoid interference from administration of the crude medicinal herbs. Eight purebred NewZealand rabbits were used for preparation of serum containing various concentrations of AETC. For- ty-eight Balb/c-nu mice were used for in vivo experi- ments. The effects of serum containing AETC on the proliferation of A549 cells and expression levels of the epidermal growth factor receptor/mito- gen-activated protein kinase （EGFR/MAPK） path- way-related proteins in vitro were investigated. Ad- ditionally, the effects on the growth of A549 xeno- grafts in nude mice, and expression levels of the EG- FR/MAPK pathway-related proteins in the xeno- grafts, were investigated. RESULTS： 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl- tetrazolium bromide （MTT） assay revealed that the serum containing AETC significantly decreased the viability of A549 cells in a dose-dependent manner. Western blot showed that the serum containing various concentrations of AETC strongly reduced the levels of phospho-Jun N-terminal kinase （p-JNK） and phospho-extracellular signal-regulated kinasel/2 （ERK1/2） while it increased the level of p-p38. However, no significant effects on the ex- pression levels of JNK, ERK1/2, and p38 MAPK were found. In addition, an anticancer effect from AETC was observed in vivo in the Balb/c-nu mice bearing A549 xenografts.CONCLUSION： AETC has significant effects on the growth of A549 xenografts and on the activity of the EGFR/MAPK pathway. Therefore, AETC may be beneficial in lung carcinoma treatment.     

Effect of Zhuyeshigao granule on tumor necrosis factor-a and inter- leukins serum protein levels in rats with radiation esophagitis

OBJECTIVE： To investigate the effects of Zhuyesh- igao granule （ZSG） on tumor necrosis factor-a （TNF-a）, interleukin-1 （IL-1）, IL-2, IL-6, and IL-8 in rats with radiation esophagitis. METHODS： Fifty Wistar rats were randomly divid- ed into five groups （10 rats in each group）： con- trol （without radiation）, saline-treated, and low, medium, and high-dose ISG-treated groups. Rats were given normal saline （10 mL/kg） or 1.15, 2.3, or 4.6 g/kg ZSG by intragastric administration once a day for 7 days. A rat model of radiation esophagi- tis was established by local irradiation of Co60 （490.25 cGy/min, totaling 30 Gy）. The administra- tion of ZSG was continued for another 7 days and on the 7th day post-irradiation, inferior vena cavablood was collected. The serum was separated, and TNF-a, IL-1, IL-2, 11_-6, and IL-8 protein levels were determined. RESULTS： Inflammatory response factors were found in the serum of each group. However, levels in ZSG-treated groups were significantly lower than in the saline-treated group （P〈0.05）. CONCLUSION： ZSG may prevent the development of radiation esophagitis, perhaps by inhibiting the generation and release of the inflammatory re- sponse factors TNF-a, IL-1, IL-2, IL-6, and IL-8.     

Clinical Application of Yongquan （KI 1）Acupointd

Yongquan （KI 1） is the Jing-Well point of Kidney Meridian of Foot Shaoyin. It can be used to treat many kinds of diseases, such as parietal headache, vertigo and blurred vision, hypopharynx swelling and pain, dry tongue, voice loss, epistaxis, apoplexy and apoplectic sequela, dysufia, ungratifying defecation, epilepsy, syncope, planter fever, palpitations, lower limbs spasm, hypertension, vomiting, infantile convulsion. The author applied Yongquan （KI 1） in clinical practice and obtained satisfactory therapeutic effects. Now five cases were introduced as follows.     

Effects of Chinese herbs combined with in vitro fertilization and em- bryo transplantation on infertility： a clinical randomized controlled trial

OBJECTIVE： To assess the effects of using Chinese herbs in assisted reproductive technology. METHODS： Four hundred and thirty-three subjects aged less than 42 years with infertility due to Fallo- pian tube or male-related factors who were willing to undertake in vitro fertilization and embryo trans- plantation were randomly allocated to a Chinese herb intervention group （n=216） or a conventional treatment control group （n=217）. All subjects re- ceived one of four routine ultra-ovulation-promot- ing therapies at the Reproductive Center in the Third Hospital Affiliated to Beijing University ac- cording to their physician＇s assessments. The sub- jects in the intervention group received various Chi- nese herbs depending on their conventional treat- ment. Endometrial thickness, number of acquired eggs, and rates of normal fertility, high-quality em- bryos, biochemical and clinical pregnancy of sub- jects were assessed in both groups.RESULTS： The high-quality embryo rate of 51.9%, biochemical pregnancy rate of 51.0%, clinical preg- nancy rate of 44.2% and endometrial thickness of （10.84± 1.75） mm in the intervention group were all significantly higher than those in the control group [48.7%, 38.9%, 34.8%, and （10.52±1.50） mm, respec- tively; P〈O.05]. The normal fertility rate of 58.5% in the Chinese herb group was also significantly supe- rior to the 54.7% achieved in the control group （P〈 0.01）. There were no statistically significant differ- ences （P〉0.05） in the average number of acquired eggs within a single cycle, incidence of excessive stimulation of ovary, rates of embryo transplanta- tion or early abortion and birth of living babies be- tween the two groups. CONCLUSION： Our findings indicate that Chinese herbs increase endometrial thickness, improve the quality of fertility and embryo, and promote embry- onic nidation, thus enhancing the success rate of in vitro fertilization/intracytoplasmic sperm injec- tion-embryo transplantation cycle. Using Chinese herbs improves th     

Oplopanax elatus（Nakai） Nakai： chemistry,traditional use and pharmacology

Oplopanax elatus（Nakai） Nakai, a member of the ancient angiosperm plant family Araliaceae, is used for the treatment of different disorders in the medicine systems of China, Russia, and Korea, and was designated in Russia as a classical adaptogen. Despite extensive studies of classical adaptogens, there are comparatively few reports concerning the chemical composition and pharmacological effects of O. elatus in English. The plant is a potential source of saponins, flavonoids, anthraquinones, terpenes, and other active compounds. Experimental studies and clinical applications have indicated that O. elatus possesses a number of pharmacological activities, including adaptogenic, anti-convulsant, anti-diabetic, anti-fungal, anti-inflammatory, anti-oxidant, blood pressure modulating, and reproductive function effects. In this review, the chemistry, safety, and therapeutic potential of O. elatus are summarized and highlighted to encourage the further development of this plant.     

Relationship between Renying pulse augmentation index and Cunk- ou pulse condition in different blood pressure groups

OBJECTIVE： To explore the relationship between Renying pulse （carotid） augmentation index （AI） and Cunkou pulse condition in different blood pres- sure groups, and the clinical significance of Reny- ing and Cunkou pulse parameters to reflect vascu- lar function. METHODS： Eighty-six patients with essential hyper- tension （EH） and 52 individuals with normal blood pressure （control group） between and January 2012 were included September 2010 this study. Reny- ing pulse AI was examined by a new diagnostic tool （ALOKA ProSound Alpha 10） --wave intensity （Wl） that is calculated as the product of the deriva- tives of the simultaneously recorded blood pres- sure changes （dP/dt） and blood-flow-velocity changes （dU/dt）, while Cunkou pulse condition was detected by DDMX-100 Pulse Apparatus inboth EH and control groups. A multifactorial corre- lation analysis was performed for data analysis. RESULTS： After adjusting for potential confound- ing variables, in the EH group, AI was positively cor- related with ts, w2/t （rts=0.225, P〈0.05; rw2/t=0.230, P〈 0.05） and negatively correlated with hs, hs/hl and w2 （rhs=- 0.393,P〈0.01 ;rhs/l=- 0.444, P〈0.01 ;rw2=- 0.389, P〈0.01）. In the control group, AI was positively cor- related with t3, t4, ts and w, （rt3=0.595, P〈0.01; r,4= 0.292, P〈0.05; rt5=0.318, P〈0.05; rw1=0.541, P〈0.01） and negatively correlated with h1, h2, h3, Ad and A （rh1= - 0.368, P〈0.05; rh2= - 0.330, P〈0.05; rh3= - 0.327, P〈 0.05; rAd=- 0.322, P〈0.05; rA=- 0.410, P〈0.01）. In the total sample group （EH plus control group, n= 138）, AI was positively correlated with t, ts, w1 and w, （rt=0.257, P〈0.01; rt5=0.266, P〈0.01; rw1=0.184, P〈 0.05; rw/t=0.210, P〈0.05） and negatively correlated with hs, hs/hl, w2 and Ad （rhs= - 0.230, P〈0.01; rh5/h1= - 0.218, P〈0.05; rw2= - 0.267, P〈0.01; rAd= - 0.246, P〈0.01）. Multiple linear regression analysis was car- ried out to model the relationship （F=7.887, P〈 0.001）.CONCLU     

Antioxidant activity and phenol and flavonoid contents of eight medicinal plants from Western Nepal

OBJECTIVE： The purpose of this study was to evaluate the antioxidant activity, phenolic and flavonoids contents of selected medicinal plants form the Western region of Nepal.METHODS： The antioxidant activity of selected medicinal plants were determined by using 2, 2-diphenyl-1-picrylhydrazyl radical scavenging activity; total ferric ion reducing ability through spectroscopic analysis. The content of total phenols was determined using Folin-Ciocalteu reagent, whereas Aluminum chloride colorimetric method was used for flavonoid determination.RESULTS： Extracts of Syzygium Operculatus（87%）,Astilbe Rivularis（83%） and Mallotus Philippnensis（88%） showed significant free radical scavenging activity with effective concentration（EC50） close to that of ascorbic acid. Syzygium Operculatus（96%）,Astilbe Rivularis（97%） and Mallotus Philippnensis（97%） had potent reducing power in concentration dependent fashion. Those plant extract with higher free radical scavenging and ferric reducing effect also showed the greater content of both phenols and flavonoids, suggesting the correlation between polyphenolic content and antioxidant activity. Those plant extracts which showed better antioxidant activity assays, also demonstrated higher total phenol and flavonoid contents. These three plants showed the presence of higher amount of phenols and flavonoids.CONCLUSION： This study may provide the scientific basis for the traditional use of those plants and may provide valuable idea for further research.     

中药膏方治疗疑难杂症验案4则

所谓膏方又称膏滋方,具有治病纠偏,滋补强身等多种作用。时下在江南一带时兴冬令以膏方进补,实际上是中药传统剂型的丸、散、膏、丹、汤、酒、露、锭八种剂型之一。由于服用方便、疗效显著,在一年四季均可服用,尤其是针对一些需长期服药的慢性病或疑难顽症,更适合以膏方来调治。现代家庭多具备冰箱冷藏的条件,这为膏剂的收藏提供了方便。因此,更可以提倡以膏方剂型来治疗诸多慢性疾病。笔者常不拘季节以膏方为主,根据辨证施治原则来治疗某些疑难病症,现撷取4则医案,以飨读者,不当之处,请多赐教为盼。     

Xinfeng capsule improves pulmonary function in ankylosing spon- dylitis patients via NF-κB-iNOS-NO signaling pathway

OBJECTIVE： To study changes in the nuclear factor-κB p65（NF-κB p65）-inducible nitric oxide synthase（i NOS）-nitric oxide（NO） signaling pathway and the effects of Xinfeng capsules（XFC） in patients with ankylosing spondylitis（AS）METHODS： One hundred twenty patients with AS were randomly divided into an XFC group and a Salazopyrin group. Sixty health subjects were included as a normal control group. In the two treatment groups, pulmonary functional parameters,forced vital capacity（FVC）, forced expiratory volume in 1 second（FEV1）, maximal voluntary ventilation（MVV）, peak expiratory flow（PEF）, forced expiratory flow at 25% of forced vital capacity（FEF25）,forced expiratory flow at 50% of forced vital capacity（FEF50）, and forced expiratory flow at 75% of forced vital capacity（FEF75） were determined. Enzyme linked immunosorbent assays were used for detection of the serum oxidative stress indexes,NF-κB p65, i NOS, NO, reactive oxygen species（ROS）, reactive nitrogen species（RNS）, malondialdehyde（MDA）, superoxide dismutase（SOD）, catalase（CAT）, total antioxidative capacity（TAOC） and interleukin-4（IL-4）, IL-10, IL-1β, and tumor necrosis factor-α（TNF-α） contents. Westergren＇s method was used for determination of erythrocyte sedimentation rate（ESR）. High-sensitivity C-reactive protein（Hs-CRP） was detected with a 7060 full-automatic biochemical analyzer（Hitachi, Japan）.RESULTS： The clinical therapeutic effect in the XFC group was significantly superior to that in the Salazopyrin group（P〈0.01）. Compared with the normal control group, FEV1, MVV, PEF, FEF50, FEF75, SOD, CAT,TAOC, IL-4, IL-10 were significantly lower, and NF-κB p65, i NOS, NO, ROS, RNS, MDA, IL-1β, TNF-α, ESR,and Hs-CRP significantly higher in patients with AS（P〈0.01 or P〈0.05）. Compared with before treatment, FEV1, MVV, PEF, FEF50, FEF75, SOD, CAT, TAOC,IL-4, and IL-10 were significantly increased, and NF-κB p65, i NOS, NO, ROS, RNS, MDA, IL-1?     

Polysaccharides from Tricholoma matsutake and Lentinus edodes enhance 5-fluorouracil-mediated H22 cell growth inhibition

OBJECTIVE： Few studies have investigated the ef- fects produced by combinations of polysaccharides and chemotherapeutic drugs in cancer treatment. We hypothesized that a combination of polysaccha- rides （COP） from Lentinus edodes and Tricholoma matsutake would improve the efficacy of 5-fluoro- uracil （5-FU）-mediated inhibition of H22 cell growth. METHODS： Mice were injected H22 cells and then treated with either 5-FU, polysaccharides from Tri- choloma matsutake （PTM）, polysaccharides from Lentinus edodes （PL）, PTM ＋ PL, 5-FU ＋ PTM, 5-FU ＋ PL, or 5-FU ＋ COR The tumor weight and volume, and splenic CD4 ＋ and CD8 ＋ T cell frequencies, were determined. Additionally, splenic natural killer （NK） cell and cytotoxic T lymphocyte （CTL） activities were assessed and the serum levels of tumor necro- sis factor-a （TNF-a）, Interleukin-2 （IL-2）, and Interfer- on-y （IFN-y） were measured. RESULTS： Compared with mice from the control,5-FU, PL, PTM, PTM ＋ PL, 5-FU ＋ PL, and 5-FU ＋ PTM groups, mice treated with 5-FU ＋ COP showed： （a） significantly reduced tumor weight and volume （P〈 0.05）, （b） significantly higher serum levels of TNF-α, IL-2, and IFN-y （P〈0.05）, （c） significantly increased CD4＋ and CD8＋ 1 cell frequencies in the spleen （P〈 0.05）, and （d） significantly increased splenic NK cell and CTL activities （P〈0.05）. The tumor weight and volume in mice treated with 5-FU＋PL or 5-FU＋PTM were significantly reduced compared with mice treated with 5-FU alone （P〈0.05）. Serum levels of TNF-α, 11.-2, and IFN-y, frequencies of CD4 ＋ and CD8＋ T cells in the spleen, and splenic NK and CTL activities were also significantly increased in mice treated with 5-FU＋PL or 5-FU＋PTM compared with mice treated with 5-FU alone （P〈0.05）. CONCLUSION： Polysaccharides from Lentinus edodes and Tricholoma matsutake could enhance the efficacy of 5-FU-mediated H22 cell growth inhi- bition.     

刘公望运用经方治疗放化疗不良反应验案2则

恶性肿瘤的致死率甚高,源自上皮组织者称为癌,源自间叶非上皮组织者称为肉瘤,二者均病情凶险,预后恶劣。目前肿瘤的治疗有外科手术治疗、放射治疗及化学治疗。在放化疗过程中往往发生毒副作用,导致患者的抵抗力下降,严重时可影响治疗的继续进行。刘公望教授巧用经方治疗放化疗后引起的毒副反应,收效良好。现举医案2则,以飨读者。     

Huogu Ⅰ formula prevents steroid-induced osteonecrosis in rats by down-regulating PPARy expression and activating Wnt/LRP5/ i3-catenin signaling

OBJECTIVE： To investigate the effects of Huogu I formula on regulation of lipid metabolism in ste- roid-induced osteonecrosis of the femoral head （SONFH） rats and verify our hypothesis that Huogu I formula regulates lipid metabolism by down-regulating peroxisome proliferator-activated receptor gamma （PPARy） expression and activating Wnt signaling pathways. METHODS： Eighty-five rats were divided into four groups： control, model, Huogu 15 g/kg and Huogu 30 g/kg. Six weeks later, animals were anaesthe- tized, femora were dissected for histopathologicalexamination of the osteonecrotic changes and re- pair processes, micro computed tomography （Mi- cro-CT）-based micro-angiography was performed to assess vascularization. Serum lipid levels were detected by haematological examination. The ex- pressions of PPARy, Wnt3a, low density lipoprotein receptor-related protein 5 （LRP5） and 13-catenin were evaluated by immunohistochemistry, Western blot and quantitative real-time polymerase chain reaction analyses. RESULTS： The incidence of osteonecrosis, ratio of empty lacuna, adipose tissue area and adipocyte perimeter in the bone marrow were dramatically lower in the Huogu ~ formula treatment groups. By micro-CT quantification, Huogu ~ formula treat- ment dose-dependently increased vessel volume, vessel surface, percentage of vessel volume and vessel thickness of the femoral heads of SONFH rats. Levels of serum lipid in Huogu 15 g/kg and Huogu 30 g/kg groups reduced significantly. HuoguⅠformula treatment could suppress the ex- pression of PPARy and increase the expressions of Wnt3a, LRP5 and 13-catenin at both protein and mRNA levels. CONCLUSION： The results of our present study highlight the lipid-lowering potential of Huogu Ⅰ formula, and provide further evidence of the in- volvement of the PPARy inhibition and Wnt/LRPS/ 13-catenin signaling activation in the effects of Huogu Ⅰ formula.     

Antidepressant-like effect of essential oil of Perilla frutescens in a chronic,unpredictable, mild stress-induced depression model mice

Perilla frutescens（Perilla leaf）, a garnishing vegetable in East Asian countries, as well as a plant-based medicine, has been used for centuries to treat various conditions, including depression. Several studies have demonstrated that the essential oil of P. frutescens（EOPF） attenuated the depressive-like behavior in mice. The present study was designed to test the anti-depressant effects of EOPF and the possible mechanisms in an chronic, unpredictable, mild stress（CUMS）-induced mouse model. With the exposure to stressor once daily for five consecutive weeks, EOPF（3, 6, and 9 mg·kg-1） and a positive control drug fluoxetine（20 mg·kg-1） were administered through gastric intubation to mice once daily for three consecutive weeks from the 3rd week. Open-field test, sucrose consumption test, tail suspension test（TST）, and forced swimming test（FST） were used to evaluate the behavioral activity. The contents of 5-hydroxytryptamine（5-HT） and its metabolite, 5-hydroxyindoleacetic acid（5-HIAA）, in mouse hippocampus were determined by HPLC–ECD. Serum interleukin（IL）-1, IL-6, and tumor necrosis factor（TNF）-α levels were evaluated by enzyme-linked immunosorbent assay（ELISA）. The results showed that CUMS significantly decreased the levels of 5-HT and 5-HIAA in the hippocampus, with an increase in plasma IL-6, IL-1β, and TNF-α levels. CUMS also reduced open-field activity, sucrose consumption, as well as increased immobility duration in FST and TST. EOPF administration could effectively reverse the alterations in the concentrations of 5-HT and 5-HIAA; reduce the IL-6, IL-1β, and TNF-α levels. Moreover, EOPF could effectively reverse alterations in immobility duration, sucrose consumption, and open-field activity. However, the effect was not dose-dependent. In conclusion, EOPF administration exhibited significant antidepressant-like effects in mice with CUMS-induced depression. The antidepressant activity of EOPF might be related to the relation between alteration of seroto     

Characteristics of Mang Needle Therapy and Its Clinical Application

Mang needle （elongated needle, 芒针） is evolved from the long needle, one kind of nine needles in the ancient times, and is primarily characterized by its long needle-body and its arrival at the deep tissues of the body. However, because of the particularity of the physiology and anatomy of some diseases, direct deep puncturing can＇t reach the due tissues, thus the treatment results are affected. Based upon the deep puncturing of Mang needle and the physiology and anatomy of the human body, professor YANG Zhao-gang, a well-known acupuncturist, has made many improvements and innovations, in his long-term clinical practice, in the manipulations and indications of Mang needle, consequently to establish a series of unique puncturing techniques of Mang needle, which work excellently on some difficult and complicated diseases. This Mang needle therapy is introduced as follows.     

Cytological and biochemical studies during the progression of alloxan-induced diabetes and possible protection of an aqueous leaf extract of Costus afer

Some plants have proven efficacy in the management of diabetes mellitus, of which Costus afer is one. This study was designed to evaluate the cytological and biochemical properties, and comparative ameliorating effects, of an aqueous extract of Costus afer Ker Gawl.（Costaceae） leaf and glibenclamide（GBM）, in liver, kidney, and pancreatic injury induced by alloxan. Thirty male albino rats were divided into six weight-matched groups. Group one served as the negative control（non-induced and non-treated, control）, while groups 2–6 were alloxan-induced diabetic groups. Group 2 served as a positive control（induced and non-treated, IC）, groups 3–5 were treated with different doses of the extract（375, 750, and 1 125 mg/kg body weight） and glibenclamide, respectively. Body weight, absolute and relative organ weights, food and fluid intake, levels of serum glucose and liver enzymes and kidney parameters were calculated and compared. Hepatocytes, renal tubules, and pancreatic cells of diabetic rats, in diabetic non-treated and treated rats were harvested and examined histopathologically. There was dose dependent amelioration on the injuries induced by alloxan on both hepatocytes, renal tubules, and pancreatic cells after treatment with Costus afer. The glucose level was reduced significantly in the Costus afer treated diabetic rats compared with the non-treated diabetic group. Costus afer leaves seem to be effective against diabetic cell injury induced in rat liver, kidney, and pancreas.     

Protective effect of Tanreqing injection on axon myelin damage in the brain of mouse model for experimental autoimmune encephalo- myelitis

OBJECTIVE： To evaluate the effect of Tanreqing injection on axon myelin in the mouse brain of experimental autoimmune encephalomyelitis（EAE）.METHODS： An EAE model was established by myelin oligodendrocyte glycoprotein（MOG）35-55 immunization in C57BL/6 mice. Mice were randomly divided into the following groups： normal, model,prednisone acetate（PA）（6 mg/kg）, Tanreqing high dose（5.14 m L/kg）, Tanreqing low dose（2.57 m L/kg）. On the day of immunization, both Tanreqing groups were treated by intraperitoneal injection,with the PA group treated by intragastrical perfusion after T cell response, and the other groups treated with saline. Changes in body weight, neurological deficit score, incidence rate, mortality rate,and course of disease were observed for all mice.Brain tissue was isolated and stained with hematoxylin-eosin, and pathological investigations performed to evaluate axon myelin damage by transmission electron microscopy（TEM）. Myelin basic protein and microtubule associated protein-2 were analyzed by immunohistochemistry.RESULTS： Tanreqing injection significantly prolonged EAE latency and decreased the neurological deficit score, alleviated infiltration of inflammatory cells in the focus area, up-regulated hippocampal MBP expression at the acute stage and the remission stage, and increased microtubule associated protein-2 expression in the EAE brain to varying degrees in the acute stage. TEM analysis indicated that Tanreqing injection alleviates myelin damage in the EAE mouse and maintains the integrity of circular layer structures and alleviates axon mitochondrial swelling.CONCLUSION： Tanreqing injection alleviates EAE symptoms.     

Clinical curative effect of fuzi-cake-separated moxibustion for pre- venting dysuria after operation for lower limb fracture

OBJECTIVE： To assess the clinical curative effect of fuzi-cake-separated moxibustion at Zhongji（CV 3）and Guanyuan（CV 4） for preventing dysuria after internal fixation of lower limb fractures.METHODS： Sixty patients conforming to the inclusion standards were randomly divided into a treatment group（n=30） and a control group（n=30）.Fuzi-cake-separated moxibustion was performed at Guanyuan（CV 4） and Zhongji（CV 3）, 20 min at a time, twice a day, for 3 days before operation in the treatment group. No fuzi-cake-separated moxibustion was performed in the control group. After treatment, the score for symptoms of first urination, urinary time, urinary volume, 24 h remaining urinary volume, incidence of uroschesis, and rate of controlling dysuria were compared to evaluate the curative effect of preventing post-operative dysuria.RESULTS： The score for symptoms of first urination,24 h remaining urinary volume（maximum 120 m L vs 250 m L, and less than 10 m L in 24 cases vs 15 cases）, and the rate of controlling dysuria（83.34% vs30%） were significantly better（P〈0.05, P〈0.05, and P〈0.001, respectively） in the treatment compared with the control group. There was no statistical difference（P〉0.05） between the two groups in first post-operative urinary time, urinary volume, or incidence of 24 h uroschesis.CONCLUSION： Fuzi-cake-separated moxibustion at Zhongji（CV 3） and Guanyuan（CV 4） can better prevent post-operative dysuria, effectively promote the functional restoration of the urinary bladder,and control the incidence of post-operative dysuria.