龋病患儿头发中微量元素含量分析

目的观察人头发中微量元素与龋病之间的关系。方法应用能量色散X射线荧光分析技术，对120例龋病患儿和50例正常对照者头发中的锌(Zn)、钙(Ca)、铅(Pb)、铁(Fe)、铜(Cu)等5种微量元素进行测定。结果120例龋病患儿头发中Zn、Ca、Fe、Cu含量明显低于正常对照组(P＜0．05或P＜0．01)，Pb含量明显高于正常对照组(P＜0．05)。结论龋病患儿与头发中的微量元素含量存在一定的关系。通过检测头发中Zn、Ca、Pb、Fe、Cu等不同微量元素的含量，可判断小儿龋病的发生、发展和预后状况。     

复发性口腔溃疡患者头发中微量元素的分析

目的 明确体内微量元素与复发性口腔溃疡之间的关系。方法 测定84例复发性口腔溃疡患者及50例正常人头发中Zn、Cu、Fe、Mn、Mg的含量并加以比较。结果 复发性口腔溃疡患者发锌含量低于正常人（P＜0．01）,发铜含量高于正常人P＜0．05）,铁、锰、镁含量与正常人无明显差异（P＞0．05）。结论 复发性口腔溃疡的发生及复发与体内少锌高铜有关。因此,可适当补充锌制剂。     

检测糖尿病患者血清微量元素的临床意义

【摘要】目的通过检测糖尿病患者血清中微量元素的含量,探讨微量元素对糖尿病的影响。方法使用等离子体发射光谱仪测定154例糖尿病患者血清中微量元素锌、铜、硒、铬含量,并与健康体检者（对照组）比较。结果糖尿病患者血清中微量元素锌、硒、铬的含量低于对照组,铜的含量明显高于对照组,差异均具有统计学意义（P〈0．01）。并且糖尿病肾病及血糖控制差的患者锌、硒、铬水平更低,而血铜含量则更高,差异具有统计学意义（P〈0．0l,P〈0．05）。结论糖尿病患者体内微量元素的含量与糖尿病具有密切关系。适当调整患者体内微量元素的含量将有助于糖尿病病情的控制。     

示波极谱法测定耳垂血中微量元素的研究

采用示波极谱仪对80名学龄前儿童及40名健康成年人耳垂血中微量元素（TE）锌、铁、铜、锰（Zn、Fe、Cu、Mn）进行测定。结果，儿童耳垂血中Zn、Fe含量低于成年人；Cu含量高于成年人（P均＜0．01）；Mn含量两者比较无差异（P＞0．05）。本法测定操作简便、数据准确、采血量小、患者痛苦少，易于接受。     

厌食儿发锌、铁、铜测定分析(附110例病例报告)

本文对110例厌食儿及健康儿行发锌、铁、铜含量测定。结果显示厌食儿发锌含量明显低于健康儿，P＜0．01；而发铜、铁含量基本正常，两组间差异无显著性，故提示厌食是缺锌的最早症状之一。同时对两组间Cu／Zn比值进行比较，结果表明厌食组明显高于健康组（P＜0．01）说明体内微量元素变化以锌明显，Cu、Zn含量呈负相关。     

吉林地区儿童视力低下与头发锌、铁、铬和铜含量的关系研究

目的：探讨学龄儿童头发锌、铁、铬、铜含量与视力低下的相关性．方法：调查298名小学生的标准对数视力，双眼裸视力≥1．0者为正常，双眼裸视力〈1．0者为视力低下．原子吸收光谱法测定头发锌、铁、铬、铜的含量．结果：市区重点小学学生视力低下率明显高于郊区普通小学学生（P〈0．001）．视力低下组锌、铁、铬含量低于正常组，分别为P〈0．001、P〈0．05、P〈0．001，其中，重点小学学生视力低下组锌、铬含量低于正常组（均P〈0．05）；普通小学生视力低下组锌、铁、铬低于正常组（均P〈0．05）．结论：头发锌、铁、铬含量与儿童视力低下可能相关．提示应关注儿童视力健康与微量元素等均衡营养的关系．     

三氯化铝染毒小鼠血清和脾脏微量元素锌、铜含量测定及其意义

①目的 测定三氯化铝（AlCl3)染毒小鼠血清和脾脏微量元素Zn、Cu含量,从微量元素角度探讨铝对小鼠免疫功能的影响。②方法 实验用健康成龄小鼠30只,随机分为3组,正常对照组（简称对照组）、三氯化铝低剂量染毒组（简称低铝组）、三氯化铝高剂量染毒组（简称高铝组）。实验结束后测定血清和脾脏中微量元素锌、铜含量。③结果 三氯化铝使小鼠血清和脾脏锌含量下降。与对照组比较,低铝组为（P＜0．05）、高铝组为（P＜0．01）;血清铜含量虽有下降趋势,但无统计学差异,而脾脏铜则上升;高铝组与对照组比较差异显（P＜0．05）。④结论 铝可致小鼠免疫功能下降。     

心绞痛发作时血清锌、铜含量的临床意义

目的 探讨心绞痛发作时血清锌、铜含量变化的临床意义。方法 心绞痛病人发作时和缓解后分别采静脉血4ml,用高频等离子发射光谱法测定血清中微量元素锌、铜浓度。结果 心绞痛发作时血清中锌浓度较对照组明显降低（P＜0．01）,而血清铜浓度明显增高（P＜0．01）;心绞痛缓解后血清锌、铜浓度较对照组均有差异（P＜0．01,P＜0．001）;心绞痛缓解后锌浓度较发作时明显回升（P＜0．01）,而血清铜浓度明显降低（P＜0．001）;心绞痛发作时血清铜锌比值明显升高（P＜0．01）,而缓解后血清铜锌比值较对照组无差异（P＞0．05）,心绞痛发作时血清铜锌比值较心绞痛缓解后有明显差异（P＜0．01）。结论 血清中微量元素锌铜水平与心绞痛发作和缓解存在明显相关性,揭示微量元素锌、铜在心绞痛发生机理上起重要作用。     

女性不孕症患者头发中锌,铜含量分析

检测158例女性不孕症患者和50例正常人头发中微量元素锌、铜的含量，旨在探讨微量元素对不孕症的影响。结果发现不孕症组发锌较正常人组低（P＜0．01），而不孕症组发铜较正常人组高（P＜0．01）。表明锌低铜高影响女性受孕。     

糖尿病大鼠组织中微量元素分析

目的 研究糖尿病大鼠组织内微量元素与糖尿病的关系。方法 用原子吸收分光光度法测定实验大鼠肝、骨、毛发、皮中的微量元素Cu、Fe、Mg、Zn、Ca。结果 与正常对照组相比,糖尿病大鼠体重减少（P〈0．01）,肝钙降低（P〈0．01）、肝锌升高（P〈0．01）、毛铜降低（P〈0．05）、毛镁升高（P〈0．05）、骨铁锌升高（P〈0．05）、皮锌升高（P〈0．01）。结论 糖尿病大鼠伴有体内严重的的钙流失,锌在肝、骨、皮中,铁在骨中有代谢障碍而导致沉积。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.