Pattern-motion responses in human visual cortex.

Physiological models of visual motion processing posit that 'pattern-motion cells' represent the direction of moving objects independent of their particular spatial pattern. We performed fMRI experiments to identify neuronal activity in the human brain selective for pattern motion. A protocol using adaptation to moving 'plaid' stimuli allowed us to separate pattern-motion responses from other types of motion-related activity within the same brain structures, and revealed strong pattern-motion selectivity in human visual area MT+. Reducing the perceptual coherence of the plaids yielded a corresponding decrease in pattern-motion responsivity, providing evidence that percepts of coherent motion are closely linked to the activity of pattern-motion cells in human MT+.     

Effects of blocking non-N-methyl-D-aspartate receptors on visual responses of neurons in the cat visual cortex

To elucidate the function of non-N-methyl-D-aspartate types of glutamate receptors in the primary visual cortex of the adult cat, we studied the effects of the iontophoretically applied glutamate receptor antagonists 6-cyano-7-nitroquinoxaline-2,3-dione and D-amino-5-phosphonovalerate. Antagonists were applied with ejecting currents that selectively blocked non-N-methyl-D-aspartate receptors. Among 93 cells in which stable recordings were obtained, 6-cyano-7-nitroquinoxaline-2,3-dione reduced the visual response in all cells. The average response magnitude during 6-cyano-7-nitroquinoxaline-2,3-dione administration was reduced to 11.7% of the control (average ejecting current: 41.2 nA). The effect of 6-cyano-7-nitroquinoxaline-2,3-dione was obvious throughout all cortical layers. The effect of D-amino-5-phosphonovalerate on the visual response was tested in 14 cells and it was also effective in blocking the visual response: the average response magnitude during D-amino-5-phosphonovalerate administration was 45.0% of the control (average ejecting current: 41.4 nA). The effect of 6-cyano-7-nitroquinoxaline-2,3-dione on the response was compared in individual cells at both high and low firing rates in order to determine whether a differential effect exists on the level of firing activity of cells due to secondary inactivation of voltage-dependent N-methyl-D-aspartate receptors. However, no indication of response dependency on firing rate was seen with 6-cyano-7-nitroquinoxaline-2,3-dione. We suggest that excitatory transmission at the geniculocortical and corticocortical synapses seems to be strongly dependent on non-N-methyl-D-aspartate receptors throughout the primary visual cortex of the adult cat, and that both non-N-methyl-D-aspartate and N-methyl-D-aspartate type glutamate receptors function additively.     

Effects of gaze on apparent visual responses of frontal cortex neurons

Previous reports have argued that single neurons in the ventral premotor cortex of rhesus monkeys (PMv, the ventrolateral part of Brodmann's area 6) typically show spatial response fields that are independent of gaze angle. We reinvestigated this issue for PMv and also explored the adjacent prearcuate cortex (PAv, areas 12 and 45). Two rhesus monkeys were operantly conditioned to press a switch and maintain fixation on a small visual stimulus (0.2° × 0.2°) while a second visual stimulus (1° × 1° or 2° × 2°) appeared at one of several possible locations on a video screen. When the second stimulus dimmed, after an unpredictable period of 0.4–1.2s, the monkey had to quickly release the switch to receive liquid reinforcement. By presenting stimuli at fixed screen locations and varying the location of the fixation point, we could determine whether single neurons encode stimulus location in absolute space or any other coordinate system independent of gaze. For the vast majority of neurons in both PMv (90%) and PAv (94%), the apparent response to a stimulus at a given screen location varied significantly and dramatically with gaze angle. Thus, we found little evidence for gaze-independent activity in either PMv or PAv neurons. The present result in frontal cortex resembles that in posterior parietal cortex, where both retinal image location and eye position affect responsiveness to visual stimuli.     

Slow adaptation in fast-spiking neurons of visual cortex

Fast-spiking (FS) neurons are a class of inhibitory interneurons classically characterized as having short-duration action potentials (<0.5 ms at half height) and displaying little to no spike-frequency adaptation during short (<500 ms) depolarizing current pulses. As a consequence, the resulting injected current intensity versus firing frequency relationship is typically steep, and they can achieve firing frequencies of < or =1 kHz. Here we have investigated the properties of FS neurons discharges on a longer time scale. Twenty second discharges were induced in electrophysiologically identified FS neurons by means of current injection either with sinusoidal current or with square pulses. We found that virtually all FS neurons recorded in cortical slices do show spike-frequency adaptation but with a slow time course (tau = 2-19 s). This slow time course has precluded the observation of this property in previous studies that used shorter pulses. Contrary to the classical view of FS neurons functional properties, long-duration discharges were followed by a slow afterhyperpolarization lasting < or =23 s. During this postadaptation period, the excitability of the neurons was decreased on average for 16.7 +/- 6.8 s, therefore rendering the cell less responsive to subsequent afferent inputs. Slow adaptation is also reported here for FS neurons recorded in vivo. This longer time scale of adaptation in FS neurons may be critical for balancing excitation and inhibition as well as for the understanding of cortical network computations.     

Dendritic spine and dendritic field characteristics of layer V pyramidal neurons in the visual cortex of fragile-X knockout mice

Fragile-X syndrome is a common form of mental retardation resulting from the inability to produce the fragile-X mental retardation protein. The specific function of this protein is unknown; however, it has been proposed to play a role in developmental synaptic plasticity. Examination of human brain autopsy material has shown that fragile-X patients exhibit abnormalities in dendritic spine structure and number, suggesting a failure of normal developmental dendritic spine maturation and pruning in this syndrome. Similar results using a knockout mouse model have previously been described; however, it was noted in retrospect that the mice used in that study may have carried a mutation for retinal degeneration, which may have affected cell morphology in the visual cortex of those animals. In this study, dendritic spines on layer V pyramidal cells of visual cortices, taken from fragile-X knockout and wild-type control mice without the retinal degeneration mutation and stained using the Golgi-Cox method, were investigated for comparison with the human condition. Quantitative analyses of the lengths, morphologies, and numbers of dendritic spines, as well as amount of dendritic arbor and dendritic branching complexity, were conducted. The fragile-X mice exhibited significantly more long dendritic spines and significantly fewer short dendritic spines than control mice. Similarly, fragile-X mice exhibited significantly more dendritic spines with an immature-like morphology and significantly fewer with a more mature type morphology. However, unlike the human condition, fragile-X mice did not exhibit statistically significant dendritic spine density differences from controls. Fragile-X mice also did not demonstrate any significant differences from controls in dendritic tree complexity or dendritic arbor. Long dendritic spines with immature morphologies are characteristic of early development or a lack of sensory experience. These results are similar to those found in the human condition and further support a role for the fragile-X mental retardation protein specifically in normal dendritic spine developmental processes. They also support the validity of these mice as a model of fragile-X syndrome.     

Critical flicker frequency responses in visual cortex

Critical flicker frequency (CFF) threshold is defined as the frequency at which a flickering light is indistinguishable from a steady, non-flickering light. CFF is useful for assessing the temporal characteristics of the visual system. While CFF responses are believed to reflect activity in the central visual system, little is known about how these temporal frequencies are processed in the visual cortex. The current paper estimated the CFF threshold for cells in the rat visual cortex by recording single unit responses to flickering stimuli. Results showed that: (1) there was a broad range of temporal tuning, (2) CFF threshold was lower in simple cells than in complex and hypercomplex cells, and (3) there was no significant difference in CFF threshold between areas 17 and 18. Electronic Publication     

Vasoactive intestinal polypeptide-immunoreactive neurons in rat visual cortex

An antibody to vasoactive intestinal polypeptide (VIP) was used to examine the forms of VIP-positive neurons and the synapses made by VIP-positive axon terminals. Vasoactive intestinal polypeptide-positive cells are most common in layers II and III and the majority of them are typical bipolar neurons, with two primary dendrites which emanate from the upper and lower poles of the cell body. Their somata, which have only a few symmetric and asymmetric synapses, generally have a fusiform or "tear-drop" shape and contain nuclei with a vertically oriented cleft. The dendritic trees are arranged vertically and often extend through five cortical layers. The axons are thin and extend either from the soma or from one of the primary dendrites. The axons also follow a vertical trajectory. Other VIP-positive neurons are modified bipolar cells and a few of them are multipolar cells. The synapses formed by the VIP-positive axon terminals in the neuropil are symmetric in form, and although the synaptic clefts are narrow, the junctions are usually long and continuous, rather like those described for asymmetric synapses. Most of the VIP-positive axon terminals synpase with small dendritic shafts, but a few synapse with neuronal cell bodies. Since the majority of the VIP-positive neurons are bipolar cells it is concluded that these are the source of most of the VIP-positive axon terminals. If this is so, then the VIP-positive bipolar cells form symmetric synapses. This is in contrast to the observations of Peters and Kimerer (1981. J. Neurocytol. 10, 921-946) for the bipolar cells they examined in a Golgi-electron microscopic study had axon terminals forming asymmetric synapses. It is suggested that this disparity can be reconciled if it is assumed that the bipolar cell population consists of subgroups which have different biochemical characteristics and different synaptic relationships.     

Interhemispheric asymmetry of associative responses in the cat parietal cortex

The asymmetry of associative responses in the parietal region of the cortex was experimentally studied during visual and auditory stimulation of tubocurarineimmobilized cats at various intensities with the multiple recording of evoked potentials (EP). The left-handed asymmetry of the early and late components of associative responses was established. It was shown that the zones of convergence of visual and auditory information are also asymmetric and predominate in the left hemisphere. By contrast to the projection regions, no strength or transcallosal modulation of the interhemispheric asymmetry could be found in the parietal cortex. The data obtained indicate the applicability of the principle of functional asymmetry to the activity of paired segments of associative cortical systems in animals.Translated from Fiziologicheskii Zhurnal SSSR imeni I. M. Sechenova, Vol. 68, No. 6, pp. 729–737, June, 1982.     

Receptive field properties of single neurons in rat primary visual cortex.

The rat is used widely to study various aspects of vision including developmental events and numerous pathologies, but surprisingly little is known about the functional properties of single neurons in the rat primary visual cortex (V1). These were investigated in the anesthetized (Hypnorm-Hypnovel), paralyzed animal by presenting gratings of different orientations, spatial and temporal frequencies, dimensions, and contrasts. Stimulus presentation and data collection were automated. Most neurons (190/205) showed sharply tuned (相似文献   

Organization of suppression in receptive fields of neurons in cat visual cortex.

1. The response to an optimally oriented stimulus of both simple and complex cells in the cat's striate visual cortex (area 17) can be suppressed by the superposition of an orthogonally oriented drifting grating. This effect is referred to as cross-orientation suppression. We have examined the spatial organization and tuning characteristics of this suppressive effect with the use of extracellular recording techniques. 2. For a total of 75 neurons, we have measured the size of each cell's excitatory receptive field by use of rectangular patches of drifting sinusoidal gratings presented at the optimal orientation and spatial frequency. The length and width of these grating patches are varied independently. Receptive-field length and width are determined from the dimensions of the smallest grating patch required to elicit a maximal response. 3. The extent of the area from which cross-orientation suppression originates has been measured in an analogous manner. Each neuron is excited by a patch of drifting grating the same size as the receptive field. The response to this stimulus is modulated by a superimposed patch of grating having an orthogonal orientation. After selecting the spatial frequency that produces maximal suppression, the response of each cell is examined as a function of the length and width of the orthogonal (suppressive) grating patch. Results from 29 cells show that the dimensions of the orthogonal grating patch required to elicit maximal suppression are similar to, or smaller than, the dimensions of the excitatory receptive field. Thus cross-orientation suppression originates from within the receptive field. 4. For some cells the spatial frequency tuning of the suppressive effect is much broader than the spatial frequency tuning for excitation. In these cases it is possible to find a spatial frequency that produces suppression but not excitation. With the use of a suppressive stimulus having this spatial frequency, we examined the strength of suppression as a function of orientation for 11 cells. These tests show that suppression occurs at all orientations, including the preferred orientation for excitation. In some cases, suppression is somewhat stronger at the preferred orientation for excitation than at any other orientation. 5. For 12 cells we varied the relative spatial phase between the optimally oriented and orthogonal gratings. In all cases the magnitude of suppression is largely independent of the relative spatial phase. 6. For three binocular cells we examined whether the suppressive effect of a grating oriented orthogonal to the optimum could be mediated dichoptically.(ABSTRACT TRUNCATED AT 400 WORDS)     

Functional connectivity of disparity-tuned neurons in the visual cortex

Different mechanisms have been proposed concerning how disparity-tuned neurons might be connected to produce the signals for depth perception. Here we present neurophysiological evidence providing insight on this issue. We have recorded simultaneously from pairs of disparity-tuned neurons in the cat's striate cortex. The purpose was to determine the relationships between disparity tuning and functional connectivity revealed through neural cross-correlograms. Monosynaptic connections tend to be stronger between pairs of cells with similar disparity tuning. Pairs of complex cells tend to have either similar tuning or nearly opposite tuning with an absence of quadrature relations. Pairs with at least one simple cell do have some nearly quadrature relationships when they are recorded from the same electrode. Coarse-to-fine connections (i.e., the presynaptic cell has lower disparity frequency and larger disparity range) tend to be stronger but less frequent than those of a fine-to-coarse nature. Our results are consistent with a system that produces weighted averaging across cells that are tuned to similar disparities but different disparity scales to reduce false matches.     

Morphological evidence for callosally projecting nonpyramidal neurons in rat visual cortex

Summary This investigation shows that some of the callosally projecting neurons in rat visual cortex are nonpyramidal cells. Callosally projecting neurons were labeled by injections of horseradish peroxidase (HRP) into the area 17/18 a border zone of the contralateral hemisphere. The retrogradely transported HRP was visualized with diaminobenzidine or with tetramethylbenzidine. In some of the labeled neurons the reaction product was diffuse, so that the neurons had a Golgi-like appearance, but in others the reaction product was granular, or punctate. The majority of neurons with a Golgi-like appearance were pyramidal cells, but one callosally projecting neuron from layer V area 18 a was confirmed by electron microscopy to be a nonpyramidal neuron. This dearth of well-filled nonpyramidal cells suggested that callosally projecting nonpyramidal neurons may not transport sufficient HRP to show Golgi-like filling, and so punctately labeled neurons from areas 17, 18 a and 18 b were examined. Reacted sections from areas 17, 18 a and 18 b of control animals, into which no tracer had been injected, were also examined, but in these control preparations no granules similar to the HRP granules within the neuronal profiles of the experimental animals were encountered. In methylene blue-stained 1-m sections, neuronal profiles from the control animals possessed only blue staining lysosomes, while neuronal profiles from the experimental animals exhibited both lysosomes and HRP granules. It was determined, from the counts of HRP granules in neurons from the experimental animals, that in selected regions of areas 17, 18 a, and 18 b similar percentages of the pyramidal and nonpyramidal neuronal populations (ranging from 100% to 34%) contained HRP granules, and so had callosally projecting axons. However, most callosally projecting nonpyramidal neurons had far fewer HRP granules than the pyramidal neurons, again indicating that they transport less HRP. This could account for the fact that callosally projecting nonpyramidal neurons only rarely show a Golgi-like filling, and this could be one reason why such cells have been overlooked in most previous studies.     

Effects of cryogenic blockade of visual cortex on the responses of lateral geniculate neurons in the monkey

Summary Striate cortex in unanesthetized, paralyzed rhesus monkeys was cooled to assess the effects of temporary blockade of corticogeniculate fibers on responses of lateral geniculate neurons. Most neurons recorded under the thermode became inexcitable on cooling. After the onset of cooling, superficial layers became inexcitable before deep layers, and at a time when superficial activity was blocked, deep neurons were still orientation specific and retained this property until they themselves became inexcitable.Twenty-eight percent of the geniculate neurons projecting to cortex under the thermode were judged to be affected by cortical blockade, generally showing increases in driven and spontaneous activity. Cooling produced changes in the scale of response magnitude, rather than in the timing of impulse discharge, as evaluated by response time histograms. Responses evoked by bars or edges or by flashing spots were equally affected.The changes of activity produced were generally small and frequently difficult to differentiate from spontaneous shifts in excitability. In contrast, a visually driven inferior pulvinar neuron with a receptive field in the cooled area was strongly affected by cortical blockage, and driving quickly and completely returned soon after the cortex was rewarmed. It therefore appears that the method of blockade was effective but incapable of producing large effects in geniculate neurons. This suggests that cortical control may be inherently weak in the immobilized animal or that the distribution of activity between excitation and inhibition is equally balanced.This research was supported by National Institutes of Health Research grant 5 R01 EY00927 and by National Institutes of Health Training grant in Physiology 5 T01 GM00443     

Characteristics of the responses of visual cortex neurons with sensitivity to bars or cross-shaped figures in cats

The magnitudes and latent periods of spike responses were recorded from 280 individual neurons tuned to the orientation of light bars or cross-shaped figures in the primary visual cortex (field 17) of the cat. In control experimental conditions, half of 195 cells preferred the bar (first group), the remainder preferring crosses (second group); the responses of neurons of the first group to bars and crosses were of similar magnitude, while in the second group, responses to crosses were significantly larger than responses to bars. The latent periods of responses to optimal bars in the first group of neurons were shorter than those in the second group, and became longer on exposure to crosses, while latent periods in the second group were shorter on exposure to crosses. In conditions of local bicuculline blockade of intracortical inhibition, about a quarter of 85 neurons were sensitive only to the bar, regardless of the presence or absence of inhibition. The remaining neurons were sensitive to crosses in at least one of the states and continued to have responses which were smaller in terms of absolute magnitude than the responses of group 1 neurons. The significance of these data for understanding the mechanisms of tuning of striate neurons to signal features and the temporal sequence of their operation is discussed. __________ Translated from Rossiiskii Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 92, No. 2, pp. 152–163, February, 2006.