The Expression of Interleukin-23 （p19/p40） and Inteleukin-12 （p35/p40） in Psoriasis Skin

Interleukin-12 (IL-12), which is composed of p35 and p40 subunit, is a proinflammatory Th1-inducing cy-tokine. Besides IL-12, p40 can be covalently linked to a p35-related protein p19. This heterodimer is known as IL-23[1]. IL-12 promotes the development of naive T cells, whereas IL-23 mediates late-stage inflammation. In this study, the expression of IL-12p35/p40 mRNA and IL-23p19/p40 mRNA were analyzed by using reverse translation-polymerase chain reaction (RT-PCR) in order to pro…     

Expression of CC Chemokine Ligand 20 and CC Chemokine Receptor 6 mRNA in Patients with Psoriasis Vuigaris

Itiswell knownthatimmune inflammatorymechanism playsanimportantroleinpsoriasis .Therelationshipbetweenchemokine ,receptorsandpsori asishasbeenconfirmed[1] .CCchemokineligand 2 0(CCL2 0 )isanewmemberinthefamilyof β chemokineandalsotheonlyligandofCCchemoki…     

Study on the Expression of IL-18, AcPL and IL-18BP mRNA in the Psoriatic Lesions

Interleukine- 1 8(IL- 1 8) isa newly identified cy-tokine that plays an important role in the T- helper(Th1 ) response,primarily via its ability to induceIFN- γ production in T cells and NK cells.IL- 1 8isrelated to the IL- 1 family in terms ofstructureand interms of function,and induction of IFN-γ by IL- 1 8is similar to that by IL- 1 2 in thatthey are a sole cy-tokine,and there is synergism between IL- 1 8andIL - 1 2 for IFN-γ production[1— 4 ] .In addition,mono-cytes and macro…     

TIG2在寻常型银屑病中的表达变化及意义

目的 研究TIG2在寻常型银屑病中的作用机制.方法 用免疫组化和原位杂交的方法检测正常组织,未受累组织和银屑病皮损中TIG2蛋白和mRNA的表达变化.结果 在正常组织及未受累组织中,TIG2表达于表皮全层,在银屑病周围皮损中,可见TIG2阳性染色于棘层上部,在棘层下部及基底层中表达较少,而在银屑病中间皮损中无表达.TIG2在正常和未受累皮肤基底层中的表达高于其在银屑病边缘皮损基底层中的表达(p<0.01);TIG2在正常皮肤和未受累皮肤中的表达高于其在银屑病中间皮损中的表达(P<0.01);银屑病边缘皮损基底上层中的表达高于其在银屑病中间皮损中的表达(P<0.01).结论 TIG2可保持表皮正常分化功能,TIG2的降低可能参与银屑病的发生和发展.     

IL-15在寻常型银屑病患者皮损中的表达

目的从mRNA及蛋白质水平研究白介素15（IL-15）在寻常型银屑病患者皮损及非皮损皮肤中的表达,探讨其在银屑病发病中的作用。方法采用RT-PCR和免疫组化法对28例寻常型银屑病患者皮损及非皮损皮肤中IL-15的表达情况进行了检测,同时以10例正常人皮肤作为对照。结果2种IL-15前体蛋白的mRNA在所有皮肤标本中都有表达,银屑病皮损中IL-15 mRNA的表达明显高于非皮损处皮肤（P〈0．001）和正常人皮肤（P〈0．001）,非皮损皮肤的表达与正常人皮肤相比差异无显著性（P〉0．05）。免疫组化结果显示：IL-15低表达于非皮损和正常皮肤的基底细胞层,而在银屑病皮损处高表达,且表达范围可达表皮全层及真皮乳头层。皮损处IL-15的表达明显高于非皮损（P〈0．001）和正常皮肤（P〈0．001）,非皮损与正常皮肤之间的表达无显著性差异（P〉0．05）。结论寻常型银屑病的皮损中高表达IL-15,在疾病的发生中可能起一定的作用。     

The Expression of Interleukin-17, Interferon-gamma, and Macrophage Inflammatory Protein-3 Alpha mRNA in Patients with Psoriasis Vulgaris

Interleukin 17(IL 17)isanewlyidentifiedpro inflammatorycytokinethatisproducedbyactivatedTcells.IL 17hastheabilitytoinducestromacellstoproduceproinflammatorycytokinesandhematopoieticcytokines .Soitmayparticipateinsomeinflammatoryreactionsandsomeimmunoloreg…     

成纤维细胞生长因子10 mRNA在寻常型银屑病皮损中的表达

目的:研究成纤维细胞生长因子10 mRNA在寻常型银屑病皮损中的表达,探讨其在银屑病发病中的作用机制.方法:应用原位杂交法检测FGF10 mRNA在22例正常皮肤组织、银屑病皮损和未受累皮肤中的表达和分布.结果:原位杂交法检测发现,在22例正常皮肤组织、银屑病皮损和未受累皮肤表皮的全层或表皮中下层可见FGF10 mRNA表达(95%);在正常皮肤表皮的基底层或少量细胞内可见FGF10 mRNA的表达(5%);在寻常型银屑病非皮损表皮的中下层或个别细胞内可见FGF10 mRNA的表达(82%).寻常型银屑病皮损表皮中FGF10 mRNA的表达明显高于正常对照组(P＜0.05).结论:FGF10 mRNA在银屑病发病早期阶段的表达增高可能与银屑病的早期发病有关.     

Expression of LL-37, Human beta Defensin-2, and CCR6 mRNA in Patients with Psoriasis Vuigaris

Theinnateimmunesystemofhumanskincon tainsantimicrobial peptidesknownascathelicidins(LL 37)andhumanbeta defensins (hBD) [1] .Innor malskinthesepeptidesarenegligible ,buttheexpres sionofthemwillbetriggeredbyinjuryorinflamma tionoftheskin .Andtheirexpression…     

Expression of Plasmacytoid Dendritic Cells, IRF-7, IFN-α mRNA in the Lesions of Psoriasis Vulgaris

Psoriasis is a common and chronic inflammatory skin disease associated with both genetic and environ-mental risk factors. Recent researches have focused pri-marily on the role of innate immunity in psoriasis. Type Ⅰ interferons (IFNs), as represented by IFN-α, are promptly secreted upon the interaction between micro-bial pathogens and the immune system, and activate a variety of immune effector cells that belong to the innate as well as the adaptive immune system. Plasmacytoid dendritic ce…     

The Expression of Interleukin-22 and S100A7, A8, A9 mRNA in Patients with Psoriasis Vulgaris

In order to study the expression of interleukin-22 （IL-22） and S 100A7, A8, A9 mRNA in the skin lesions of patients with psoriasis vulgaris and their relationship, the biopsies were taken from skin lesions in 35 patients with psoriasis vulgaris and the skin of 16 normal controls, and the expression levels of 1L-22 and S 100A7, A8 and A9 mRNA were detected by semi-quantitative RT-PCR. The results showed that （1） IL-22 and S 100A8, A9 mRNA were positively expressed in the psoriatic skin lesions but negatively expressed in the normal controls; The expression level of S 100A7 was （1.133±0.040） in the psoriatic skin lesions, significantly higher than that in the normal controls （0.744±0.037, P〈0.01）. （2） There were significantly positive correlations between the expression of IL-22/S100A7 mRNA, IL-22/S100A8 mRNA, IL-22/S100A9 mRNA in the psoriasis vulgaris （r1=-0.543, r2=0.774, r3=0.621, P〈0.01）. It was concluded that IL-22 and S 100A7, A8, A9 might play important roles in the occurrence and progression of psoriasis.     

miR-21及其靶蛋白RASA1在寻常型银屑病患者皮肤组织中的表达及意义

目的 探讨miR-21在寻常型银屑病患者皮损组织、皮损旁组织以及正常健康人群皮肤组织中的表达水平,同时研究其下游靶蛋白RASA1在寻常型银屑病患者皮损组织和正常人群皮肤组织中的表达水平及意义。 方法 采用实时荧光定量PCR法检测miR-21在12例寻常型银屑病患者皮损组织、皮损旁组织以及15例正常人群皮肤组织中的表达水平,差别倍数用2-ΔΔCt值表示。利用免疫组织化学Envision法检测45例寻常型银屑病患者与20例正常人群皮肤组织中RASA1蛋白的表达情况,RASA1阳性显色主要为细胞质显示棕黄色或棕褐色颗粒。 结果 经过实时荧光定量PCR检测后分析显示:寻常型银屑病患者皮损组织中miR-21的表达水平显著高于正常皮肤组织,差异具有统计学意义(P<0.01)。免疫组化结果表明:RASA1蛋白在正常皮肤组织中的阳性表达率为60.00%,显著高于寻常型银屑病患者皮损中的阳性表达率(10.00%),差异具有统计学意义(χ2=14.009,P<0.001)。 结论 miR-21在寻常型银屑病皮损组织中的表达高于正常组织,其靶蛋白RASA1在寻常型银屑病患者皮肤组织中的阳性表达显著低于正常皮肤组织;可能是由于miR-21的差异表达调控RASA1基因,导致RASA1蛋白表达受到抑制,从而参与指向寻常型银屑病的发病过程。      

银屑病患者白介素8的观察

对４３例银屑病患者，应用ＥＬＩＳＡ法检测了白介素８（ＩＬ－８）的血清含量，同时对其中１２例患者，应用免疫用化技术，观察了ＩＬ－８在其皮损及非皮损区的染色强度。结果表明，银屑病患者血清ＩＬ－８含量明显高于正常人，增高程度与银屑病面积严重程度系数积分相关；皮损处ＩＬ－８染色强度高于非皮损区和正常人皮肤。提示ＩＬ－８在银屑病的发生发展上具有一定作用。     

寻常型银屑病患者皮损中IL-22与IL-22R1、STAT3、c-myc的mRNA表达及关系研究

目的探究白细胞介素-22(IL-22)及IL-22R1、信号转导和转录激活因子-3(STAT3)、原癌基因(c-myc)在银屑病表皮异常增殖中的作用。方法采用逆转录聚合酶链反应(RT-PCR)技术对23例寻常型银屑病患者皮损和11例正常人皮肤中IL-22、IL-22R1、STAT3、c-myc的mRNA水平进行检测。结果 IL-22、IL-22R1、STAT3、c-myc的mRNA在寻常型银屑病皮损中和正常人皮肤中均呈阳性表达;但在寻常型银屑病皮损中的表达水平较正常人组明显升高,差异有统计学意义(P0.01)。IL-22的mRNA水平与IL-22R1、STAT3的mRNA水平呈正相关(P0.05);STAT3的mRNA水平与c-myc的mRNA水平呈正相关(P0.05)。结论 IL-22可能诱导STAT3活化导致银屑病角质形成细胞(KC)过度增殖,这为银屑病治疗提供了新的靶点。     

Caspase3、Bcl-2和PCNA在银屑病皮损组织中的表达

目的　探讨银屑病发生发展中细胞增殖和凋亡相关指标表达的趋势。方法　应用免疫组化技术检测 30例寻常型银屑病皮损、邻近皮肤及 10例正常皮肤中PCNA、Bcl - 2、Caspase3的表达。结果　在正常表皮中Bcl - 2和PCNA在基底层阳性表达 ,但在寻常型银屑病皮损中Bcl- 2表达明显低于正常皮肤组 ,皮损处与邻近皮损处表达、邻近皮损处与正常皮肤表达比较有统计学差异 (均P <0 0 5 ) ;PCNA在银屑病皮损处为角质形成细胞广泛阳性表达 ,皮损处与邻近皮肤的表达、邻近皮肤与正常皮肤相比有统计学差异 (P <0 0 1) ;Caspase3在寻常型银屑病皮损处与邻近皮肤及正常皮肤的表达无统计学差异 (P >0 0 5 )。结论　银屑病时 ,PCNA过度表达 ,Bcl - 2低表达 ,表明细胞过度增殖 ,凋亡基因Caspase3表达无统计学差异     

Expression of IRF-4 and IBP in skin lesions of patients with psoriasis vulgaris

The expression of the interferon regulatory factor 4 (IRF-4) and the IRF-4-binding pro-tein (IBP) in psoriatic skin lesions was investigated. The expression of IRF-4 and IBP in skin lesions of 20 patients with psoriasis vulgaris were immunohistochemically dectected. Normal skin from 10 healthy people was used as normal control. The study showed that expression of IRF-4 was increased significantly in keratinocytes and inflammatory cells in the lesions of psoriasis vulgaris than that in the normal control. The detection revealed that IBP expression in keratinocytes, lymphocytes, hair follicles, and sebaceous glands in normal skin was significantly lower than that in the lesions of pso-riasis vulgaris (P<0.05). Both IRF-4 and IBP might be involved in the pathogenesis of psoriasis vul-garis.     

上皮钙粘素、β-连环蛋白、细胞周期素D1在进行期寻常型银屑病皮损的表达

目的 通过检测银屑病患者皮损处上皮钙粘素、β-连环蛋白和细胞周期素D1的表达,探讨其在银屑病角质形成细胞高度增殖中的意义.方法 用免疫组化方法 检测正常组织及进行期银屑病皮损处上皮钙粘素、β-连环蛋白和细胞周期素D1蛋白的表达.结果 银屑病患者皮损增生的表皮细胞上皮钙粘素和β-连环蛋白在颗粒层和基底细胞层表达明显降低,与正常人相比有显著性差异(P<0.01).而细胞周期素D1在银屑病患者皮损基底细胞层表达显著升高,并与β-连环蛋白的异常表达相关.结论 上皮钙粘素和β-连环蛋白表达的下调可能与银屑病角质形成细胞高度增殖有关.Wnt信号转导系统异常可能参与银屑病的发病机制.     

开玄解毒方通过调控S1P水平改善咪喹莫特诱导银屑病样小鼠皮损症状

目的:探讨开玄解毒方对于咪喹莫特诱导银屑病样小鼠模型的症状、体征改善及其对1-磷酸鞘氨醇(sphingosine-1-phosphate, S1P)水平的调节作用。方法:选用BALB/c小鼠25只,随机均分为正常组、模型组、甲氨蝶呤组、开玄解毒中剂量组和高剂量组,除正常组外,其余组采用外涂咪喹莫特法建立银屑病样皮损模型,连续干预7 d后取材。期间对皮损拍照并计算银屑病面积和严重程度指数(psoriasis area and severity index, PASI)评分;HE染色观察皮损病理改变及测量表皮厚度;对脾脏及胸腺拍照并称重;免疫组化法检测表皮Ki67、S1P表达;蛋白质印迹法检测皮损KRT17、Claudin、Occludin表达;qRT-PCR检测IL-17、IL-23、S1PRs mRNA表达水平。结果:与模型组比较,开玄解毒方可缓解咪喹莫特诱导的小鼠银屑病样皮损,降低PASI评分,减轻表皮增生程度(P均<0.01),改善胸腺、脾脏指数及血管增生情况(P<0.01或P<0.05),降低炎症因子IL-17、IL-23 mRNA表达(P<0.01),修...     

Up regulation of phosphatidylinositol 3-kinase in psoriatic lesions

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寻常型银屑病患者皮损中HBD-2、IL-1β和NF-κBp65表达的研究

目的：探讨HBD-2、IL-1β和NF-κBp65在寻常型银屑病皮损中的表达及其在银屑病中的作用机制。方法：采用SP免疫组化法检测45例银屑病患者皮损区、非皮损区及15例正常人皮肤中HBD-2、IL-1β和NF-κBp65的表达,对其在皮损中的表达进行相关性分析,并将结果与PASI评分进行相关性分析。结果：银屑病患者皮损区HBD-2、IL-1β、NF-κBp65的表达比非皮损区、正常人皮肤明显上调,非皮损区HBD-2、IL-1β的表达也高于正常人皮肤,差异均有统计学意义（均P〈0．05）。银屑病患者皮损中HBD-2、IL-1β和NF-κBp65的表达水平与PASI评分之间均存在正相关（r=0．867、0．865、0．821,均P〈0．05）。银屑病患者皮损中HBD-2与IL-1β、HBD-2与NF-κBp65、IL-1β与NF-κBp65表达水平之间均存在正相关（r=0．869、0．740、0．814,均P〈0．05）。结论：HBD-2、IL-1β、NF-κBp65在寻常型银屑病皮损中的表达均升高．可能共同参与银屑病的发病过程。