Vitapex治疗有瘘型慢性根尖周炎的临床疗效观察

目的：观察Vitapex治疗有瘘型慢性根尖周炎的临床疗效观察。方法临床选择50例57颗有瘘型慢性根尖周炎,根据临床治疗不同进行分组,研究组给予Vitapex糊剂,对照组给予氢氧化钙糊剂。观察治疗6个月、12个月后两组临床疗效以及根管治疗中疼痛(EIP)发生率。结果研究组与对照组患者治疗后6个月的有效率分别为92.59%、90.00%,两组差异无显著性(P>0.05),12月的有效率分别为92.59%、70.00%,两组差异有统计学意义(P<0.05)。研究组EIP发生率为7.41%,低于对照组的30.00%,差异有显著性(P<0.05)。结论 Vitapex治疗有瘘型慢性根尖周炎短期临床疗效好,且治疗过程中EIP发生率较低,值得临床推广。     

感染和炎症与骨质再生的关系

很多方法可以促进颜面部的骨质再生,大多数的干预措施都利用可植入材料或装置。由移植物导致的感染会引起和牙周炎相似的局部组织破损。牙齿的损坏由很多炎症调节子和组织破损酶所调节。报导指出生物膜和炎症调节子与组织破损相关,所以炎症参与干扰骨质修复和再生。矛盾的是,最近研究证实骨质的修复需要一些炎症前体调节子的出现。骨质损伤(如骨折或手术干扰)是由一系列事件级联产生的,还依赖炎症前调节子。如果炎症能够迅速解决,骨质修复就会发生。如果炎症持续存在(如感染性植入物或移植材料),持续的炎症反应会导致不理想的骨质形成。因此,调节子的作用是与表达的时间顺序相关的。更好的了解时间层序有利于优化再生修复结果。     

磷酸钙生物陶瓷的孔性、内部连通性及表面形态对骨质再生的影响

磷酸钙生物陶瓷是一类具有良好生物相容性和骨传导性的生物活性材料,由于其成分与骨的无机组分基本相当,是目前公认的最具前途的硬组织修复材料,将该材料制成多孔结构,更有利于新生骨组织生长所需的物质交流,促进新生骨形成,因而成为近年来生物材料的研究热点之一。本文从多孔磷酸钙生物陶瓷与组织的响应关系角度出发,评述了近年来磷酸钙生物陶瓷的孔性、孔和孔间的内部连通性及孔的表面形态对骨质再生影响的研究进展。     

奥硝唑氢氧化钙糊剂治疗难治性根尖周炎的临床观察

目的 探讨奥硝唑氢氧化钙糊剂治疗难治性根尖用炎的疗效.方法 通过随机分为两组,治疗组奥硝唑氢氧化钙糊荆组,对照组樟脑酚组.分别用奥硝唑氢氧化钙糊荆和樟脑酚进行根管内上药,再按疗效标准进行记录.结果 治疗组有效率为82.89%,对照组有效率为74.29%,两组有显著性差异(P<0.01).结论 奥硝唑氢氧化钙糊荆对难治性根尖周炎的疗效满意.     

睫状体破坏物理疗法治疗难治性青光眼的研究进展

睫状体破坏疗法在某些难治性青光眼中,为降低眼内压、减轻患者眼睛疼痛、保存残存视功能和提高生活质量起着重要的作用。就国内外几种物理疗法破坏睫状体的临床研究进展作了简要的回顾,简述了冷冻术,高强度聚焦超声术及激光术各自的特点,着重介绍了接触式半导体二极管激光经巩膜睫状体光凝术的特点。     

镍钛器械根管预备治疗慢性根尖周炎的术后反应及远期疗效

将94例慢性根尖周炎患者随机分为2组:每组47例。分别用镍钛器械和不锈钢器械进行根管预备,然后进行一次性根管治疗。术后1周镍钛器械组的疼痛反应患者明显少于不锈钢器械组,差异具有统计学意义(χ2=6.221,P=0.013);术后1个月2组的疼痛发生率差异无统计学意义(χ2=1.000,P=0.317);术后18个月2组的远期疗效经比较差异无统计学意义(χ2=0.341,P=0.559)。2组仅有3例治疗失败,其成功率为96.8%。镍钛器械根管预备治疗慢性根尖周炎术后反应小于不锈钢锉,远期疗效相好,值得临床推广。     

显微根尖手术联合引导骨再生技术治疗难治性根尖炎的临床疗效

目的 探讨显微根尖手术联合引导骨再生技术（GBR）治疗难治性根尖炎的临床疗效及对咀嚼功能的影响。方法选取2019年6月至2021年9月本院收治的难治性根尖炎患者82例作为研究对象,按照随机数字表法分为对照组和联合组,每组41例。对照组采用显微根尖手术治疗,联合组采用显微根尖手术联合GBR治疗。对比2组患者的临床疗效、咀嚼功能和骨吸收标志物[Wnt3a、骨保护素（OPG）、核因子-κB受体活化因子配体（RANKL）]水平。结果 联合组总有效率（100%）高于对照组（85.37%）,差异有统计学意义（P<0.05）。术后3、6、12个月,2组患者的咀嚼效率提高,咬合力逐渐增强（P<0.05）,且联合组上标指标均优于对照组（P<0.05）;术后3、6、12个月,2组患者的牙齿松动度均逐渐降低,且联合组患者术后3、6个月牙齿松动度低于对照组（P<0.05）。术后1周,2组患者的Wnt3a和OPG水平均升高,且联合组高于对照组（P>0.05）;2组患者的龈沟液RANKL水平均降低,且联合组低于对照组（P>0.05）。结论 采用显微根尖手术联合GBR治疗难治性根...     

脐带间充质干细胞静脉输注治疗难治性重型再生障碍性贫血疗效及对外周血Th1、Th17水平的影响

目的 探讨脐带间充质干细胞(MSC)静脉输注治疗难治性重型再生障碍性贫血(SAA)疗效及对外周血辅助性T细胞1(Th1)、辅助性T细胞17(Th17)水平的影响.方法 选取2014年2月至2019年11月在本院接受治疗的重型再生障碍性贫血患者106例作为研究对象,检测患者治疗前、治疗1个月、2个月、3个月后外周血Th1、Th17水平、血象变化及不良反应,根据疗效将其分为预后良好组和预后不良组,采用单因素和多因素分析疗效与临床因素的关系.结果 106例患者输注过程中及输注24 h内均未出现明显不良反应;治疗1年后预后随访,CR 31例(29.25％)、PR 44例(41.51％)、NR 19例(17.92％)、死亡12例(11.32％),即预后良好组75例,预后不良组31例;患者治疗1个月后外周血Th1、Th17水平无明显变化(P＞0.05),治疗2个月、3个月后外周血Th1逐渐升高,Th17逐渐降低(P＜0.05);治疗1个月、2个月、3个月后患者白细胞、中性粒细胞明显回升(P＜0.05),治疗1个月时血小板、血红蛋白回升缓慢(P＞0.05),治疗2个月、3个月后逐渐回升(P＜0.05);Pearson相关性分析显示,患者治疗前后外周血Th1与白细胞、中性粒细胞、血小板、血红蛋白水平正相关,Th17水平与白细胞、中性粒细胞、血小板、血红蛋白水平负相关(P＜0.05).经单因素和多因素分析显示,患者合并感染情况、诊断到治疗时间及治疗前Th1、Th17水平是影响患者预后的独立危险因素(P＜0.05).结论 MSC治疗SAA患者疗效较好,安全性较高,有助于SAA患者体内Th1/Th17的免疫平衡,且Th1、Th17水平改善与血象恢复密切相关,也是影响预后的重要因素.     

剖宫产术后疤痕妊娠患者经米非司酮联合B超监护下清宫术治疗的疗效分析

目的:探讨剖宫产术后疤痕妊娠患者经米非司酮联合B超监护下清宫术治疗效果.方法:选取2019年7月～2020年6月我院98例剖宫产术后疤痕妊娠患者,参照随机数字表法分为常规组(n=49)和研究组(n=49).常规组治疗方案为开腹子宫疤痕妊娠病灶切除术,研究组实施米非司酮与B超监护下清宫术联合治疗,比较两组手术相关指标(手术时间、术中出血量)、术后恢复情况[月经恢复时间、术后住院时间、β-人绒毛膜促性腺激素(β-HCG)恢复时间]、术后并发症发生率及术前、术后3d血清性激素[雌激素(E2)、孕酮(P)、血清促卵泡素(FSH)、黄体生成素(LH)]水平.结果:研究组手术时间、术中出血量低于常规组(P<0.05);研究组 β-HCG降至正常时间、月经恢复时间及术后住院时间较常规组短(P<0.05);术后3d,研究组血清LH、P水平较常规组低,E2、FSH水平较常规组高(P<0.05);研究组并发症发生率4.08％较常规组16.33％低(P<0.05).结论:米非司酮、清宫术(B超监护下)联合治疗剖宫产术后疤痕妊娠,可缩短手术时间,减少患者术中出血量,促进术后恢复,在稳定性激素水平、减少并发症发生方面具有积极意义.     

Effect of embryonic bone tissue on bone regeneration

Fragmented embryonic bone tissue stimulates bone regeneration. Bone formation starts not from implanted embryonic fragments, but in intact periosteum and endosteum containing cambial cells of the osteodifferon. In rabbits, recovery of damaged radial bone after implantation of fragmented embryonic bone tissue into bone defect was associated with a pronounced periosteal reaction and focal resorption of intact ulnar bone. Consolidation of damaged radial bone without implantation of fragmented embryonic bone tissue was incomplete in all experimental animals. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 130 No. 10, pp. 469–474, October, 2000     

脂联素对兔下颌快速牵张成骨的影响☆

背景：脂联素可参与骨代谢及成血管过程,但目前关于脂联素对牵张成骨有无促进作用尚不清楚。 目的：通过建立兔下颌快速牵张动物模型,探讨局部应用脂联素对骨牵张新骨再生的影响。 方法：16只新西兰大白兔随机摸球法均分为对照组及实验组,建立兔下颌单侧快速牵张模型,牵张速率为2 mm/d。在牵张开始的1,3,5 d,对照组及实验组分别于牵张间隙注入200 μL磷酸盐缓冲液或含有2 μg重组人脂联素的磷酸盐缓冲液。 结果与结论：两组动物牵张间隙内均可观察到新骨生成,组织学及显微CT检查显示实验组的新骨生成与钙化明显高于对照组。实验结果显示局部应用脂联素可有效促进兔下颌快速骨牵张的新骨再生。 关键词：脂联素;牵张成骨;动物模型;新骨再生;下颌骨 doi:10.3969/j.issn.1673-8225.2012.11.002      

杜仲醇提取物可促进根尖周炎模型大鼠骨组织的愈合

文题释义：杜仲：杜仲是中国传统的中药药材,其具有增加机体免疫性、强身健骨的疗效,研究表明杜仲可以促进成骨细胞的增殖并且可以促进骨组织的愈合。近年来对于杜仲的研究主要集中于其有效成分的提取及分析。杜仲醇：是杜仲的主要有效成分之一,可以增强关节炎大鼠骨组织的强度,并且具有降血压以及促进伤口愈合的能力。背景：目前关于杜仲的研究主要集中于其治疗骨关节炎能增强骨组织的愈合能力,但是关于杜仲对根尖周炎骨破坏的修复作用尚未见报道。目的：探讨杜仲醇提取物对根尖周炎大鼠组织蛋白酶K的影响。方法：选取24只SD大鼠随机分为根尖周炎组20只及对照组4只,根尖周炎组于右侧下颌第一磨牙开髓后建立根尖周炎模型,对照组不做任何处理。饲养4周后,取根尖周炎组及对照组各4只拍摄MICRO-CT,计算根尖周炎骨破坏量,确认大鼠根尖周炎动物模型构建成功。饲养第5周,将剩余16只根尖周炎大鼠随机分为杜仲醇提取物组及生理盐水组,每组8只,每天灌胃杜仲醇提取物5 mL/kg或等量生理盐水。灌胃4周后,每组取4只大鼠拍摄MICRO-CT,分析杜仲醇提取物对根尖周骨组织的修复能力,另外取4只大鼠采用免疫组化染色观察右下颌第一磨牙牙槽骨组织蛋白酶K的表达。结果与结论：①MICRO-CT结果显示,对照组骨吸收体积为(0.223±0.009) mm³,根尖周炎组骨吸收体积为(0.945±0.037) mm³,两组骨吸收量比较差异有显著性意义(P=0.00),说明大鼠根尖周炎模型构建成功;②灌胃4周后,MICRO-CT结果显示杜仲醇提取物组骨吸收体积较灌胃前减小,差异有显著性意义(P < 0.05);③灌胃4周后,免疫组化结果显示杜仲醇提取物根尖周炎骨破坏标志因子组织蛋白酶K的表达较灌胃前降低,差异有显著性意义(P < 0.05);④结果表明,杜仲醇提取物可以抑制骨破坏标志因子组织蛋白酶K的表达,并且可以促进大鼠根尖周炎骨组织的愈合。ORCID: 0000-0001-5450-7016(吴大雷)中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程     

丝氨酸/苏氨酸蛋白激酶促进慢性根尖周炎模型小鼠的骨破坏

背景:根尖周炎的主要表现为骨破坏和形成炎症性肉芽组织,研究发现丝氨酸/苏氨酸蛋白激酶(AKT)可促进破骨细胞分化.目的:观察AKT在小鼠实验性根尖周炎组织中的表达变化.方法:选用25只C57BL/6J野生型雌性小鼠,通过双侧下颌第一磨牙开髓后将髓腔暴露于口腔中的方法,建立小鼠根尖周炎动物模型.将5只未开髓的小鼠作为健康...     

Spontaneous bone regeneration following mandibular resection for odontogenic myxoma

Spontaneous bone regeneration is an uncommon condition following traumatic or iatrogenic bone loss. The factors responsible for its occurrence are yet to be fully elucidated. This report presents spontaneous bone regeneration following mandibular resection for a giant odontogenic myxoma in a 16-years-old Nigerian male. New bone formation was observed at the postoperative fourth week and has continued for one year after. Likely factors that favor this occurrence are critically examined. The advantages of spontaneous bone regeneration in resource-poor settings include low biologic and economic costs as compared to bone grafting.     

Effect of polycaprolactone scaffold permeability on bone regeneration in vivo

Successful bone tissue engineering depends on the scaffold's ability to allow nutrient diffusion to and waste removal from the regeneration site, as well as provide an appropriate mechanical environment. Since bone is highly vascularized, scaffolds that provide greater mass transport may support increased bone regeneration. Permeability encompasses the salient features of three-dimensional porous scaffold architecture effects on scaffold mass transport. We hypothesized that higher permeability scaffolds will enhance bone regeneration for a given cell seeding density. We manufactured poly-?-caprolactone scaffolds, designed to have the same internal pore design and either a low permeability (0.688×10(-7)m(4)/N-s) or a high permeability (3.991×10(-7)m(4)/N-s), respectively. Scaffolds were seeded with bone morphogenic protein-7-transduced human gingival fibroblasts and implanted subcutaneously in immune-compromised mice for 4 and 8 weeks. Micro-CT evaluation showed better bone penetration into high permeability scaffolds, with blood vessel infiltration visible at 4 weeks. Compression testing showed that scaffold design had more influence on elastic modulus than time point did and that bone tissue infiltration increased the mechanical properties of the high permeability scaffolds at 8 weeks. These results suggest that for polycaprolactone, a more permeable scaffold with regular architecture is best for in vivo bone regeneration. This finding is an important step toward the end goal of optimizing a scaffold for bone tissue engineering.     

实验性根尖周炎模型大鼠核因子?B的表达

BACKGROUND:mRNA expression level of nuclear factor-kappa B (NF-?B) in periapical periodontitis has been shown to be significantly higher than that in normal periapical tissue. OBJECTIVE:To observe the expression of NF-?B in rats with periapical periodontitis. METHODS:Twenty male Sprague-Dawley rats aged 6 weeks were enrolled to establish apical periodontitis model at the right mandibular first motar (experimental group), and the left mandibular first motar as normal control group. Five rats were randomly selected at postoperative 1, 2, 3, and 4 weeks to make frozen sections of the mandibular tissue. The morphology of the periapical tissue was observed through hematoxylin-eosin staining, and the expression level and distribution of NF-?B were detected by immunohistochemical staining. RESULTS AND CONCLUSION: (1) Hematoxylin-eosin staining showed that the apical periodontal tissue of the mandibular first molar at the right side had local inflammatory response and spread to peripheral tissues; while there was no pathological changes in the control group. (2) Immunohistochemistry results revealed that NF-?B expression increased from the 1st week to 2nd week after surgery, and then went down at the 3rd week and reached the lowest point at the 4th week; moreover, the expression level at each time exhibited significant differences (P < 0.05). The amount of NF-?B-positive cells in the experimental group was significantly higher than that in the control group (P < 0.05). (3) These findings suggest that the expression level of NF-?B holds close correlation with the process of periapical periodontitis indicating that NF-?B may participate in the inflammatory reaction of periapical periodontitis.  中国组织工程研究杂志出版内容重点：肾移植;肝移植;移植;心脏移植;组织移植;皮肤移植;皮瓣移植;血管移植;器官移植;组织工程     

Effect of venlafaxine on bone loss associated with ligature-induced periodontitis in Wistar rats

Background  

The present study investigated the effects of venlafaxine, an antidepressant drug with immunoregulatory properties on the inflammatory response and bone loss associated with experimental periodontal disease (EPD).     

Effect of sodium fluoride on bone tissue regeneration in hypodynamia]

It was demonstrated in rat experiments that a nonsupported position of the extremity attained by exarticulation of the leg in 10 to 40 days leads to a sharp inhibition of posttraumatic regeneration of bone tissue. Daily injection of 0.4 mg/100 g sodium fluoride, which was used in the clinic for the prevention and treatment of osteoporosis, inhibited still more the regenerative processes both under conditions of hypodynamia and also when the supporting function of the extremity was maintained.