Oxidative stress and antioxidants in patients with cardiogenic shock complicating acute myocardial infarction

BACKGROUND: Lipid peroxidation and derived oxidized products are being intensively investigated because of their potential to cause injury and because of their pathogenic role in several diseases. The view that an excess of lipid peroxidation products is present and is relevant in the pathogenesis of cardiogenic shock-induced damage has still not received definitive support. METHODS: To evaluate the extent of lipid peroxidation, the status of enzymatic and nonenzymatic antioxidants in patients with cardiogenic shock that complicate acute myocardial infarction (AMI) and to compare with normal subjects. RESULTS: Compared with normal subjects, cardiogenic shock patients had higher malondialdehyde, conjugated dienes and reduced activities of erythrocyte antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and lower concentrations of reduced glutathione (GSH) in erythrocyte and in plasma GSH, vitamin C, vitamin E and in beta-carotene. CONCLUSIONS: Cardiogenic shock is associated with greater than normal lipid peroxidation and with an imbalance in antioxidants' status. These results indicate that low activities of SOD, CAT, GPx and low concentrations of GSH, vitamin C, vitamin E and beta-carotene in the circulation of patients with cardiogenic shock complicating AMI may be due to increased utilization to scavenge lipid peroxides. Decrease in plasma concentrations of GSH, vitamin E and beta-carotene seems to be responsible for the elevation of lipid peroxidation in cardiogenic shock complicating AMI compared with MI.     

Lipid profile, oxidant-antioxidant status and glycoprotein components in hyperlipidemic patients with/without diabetes

BACKGROUND: Plasma lipoproteins are protected against oxidative modification by the antioxidant defense system. An imbalance in the antioxidant defense system seems to result from the accumulation of low density lipoprotein (or) very low density lipoprotein in the course of hyperlipidemia. METHODS: The lipid profile, glycoprotein components, glucose, total proteins, albumin, lipid peroxidation and antioxidant status of plasma and erythrocytes were investigated in hyperlipidemic patients and hyperlipidemic patients with diabetes on treatment with glibenclamide (or) glipizide along with normal subjects and compared. RESULTS: A significant increase was observed in the levels of total cholesterol (TC), VLDL-C, triglycerides (TG), lipid peroxidation, ceruloplasmin (CP), glycoprotein components and glucose in the hyperlipidemic patients with/without diabetes and the increase was more pronounced (except TC) in patients with diabetes. The activities of erythrocyte antioxidant enzymes such as superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and plasma concentrations of vitamins C and E, and reduced glutathione (GSH) decreased in hyperlipidemic patients with/without diabetes. GPx, CAT, vitamin C and GSH levels exhibited a further decrease in hyperlipidemic patients with diabetes. CONCLUSION: An enhanced levels of VLDL-C, TC/HDL-C ratio, TG, lipid peroxidation, glycoprotein components, and decreased concentrations of total proteins (TPs) and albumin were observed in hyperlipidemic patients with diabetes while the decrease was more marked in GSH, vitamin C, CAT and GPx among antioxidants.     

Circulating lipid peroxidation and antioxidant status in cervical cancer patients: a case-control study

Objectives: The present case-control study was conducted to investigate the status of circulating lipid peroxidation and the enzymic and nonenzymic antioxidants of cervical cancer patients.

Design and methods: Plasma thiobarbituric acid reactive substances (TBARS), conjugated dienes (CD) and the levels of antioxidants such as reduced glutathione (GSH), glutathione peroxidase (GPx), glutathione-S-transferase (GST), superoxide dismutase (SOD), vitamin C and vitamin E were estimated in circulation of thirty patients and an equal number of age matched normal subjects as control.

Results: Significantly elevated levels of plasma TBARS and CD and significantly lowered levels of GSH, GPx, GST, SOD vitamin C and vitamin E were observed in cervical cancer patients as compared to controls. Our study reveals increased lipid peroxidation and possible breakdown of antioxidant status in patients with cervical carcinoma.

Conclusion: These results indicate that low levels of GSH, GPx, GST, SOD, vitamin E and vitamin C in the circulation of cervical cancer patients may be due to their increased utilization to scavenge lipid peroxides as well as their sequestration by tumor cells. Malnutrition may also be a significant cause for the increased prevalence of cervical cancer in women with a low socioeconomic status.     

儿童超重和肥胖与血压、血脂及血糖异常的相关性

目的探讨儿童的超重和肥胖与血脂、血糖异常之间的相关性。方法测量410例健康体检儿童身高、体质量、血压,采集空腹静脉血测定血糖（Glu）、血清总胆固醇（TC）、三酰甘油（TG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）。根据BMI指数水平将410例体检儿童分为正常组和超重肥胖组,比较各组儿童血压、血脂、血糖的差异。结果儿童超重及肥胖率为17.80%。超重肥胖组儿童的血压、TC、TG、GLU、LDL-C、HDL-C水平与正常对照组比较差异有显著性（t=2.32～5.51,P〈0.01）。结论儿童时期超重及肥胖同血压、血糖、血脂异常密切相关,在进行心脑血管疾病早期预防时,应将肥胖超重儿童这一群体作为重点干预对象,积极预防和治疗。     

Role of selenium in reducing hypoxia-induced oxidative stress: an in vivo study.

At high altitudes, the reactive oxygen species are continuously generated as a consequence of low oxygen partial pressure (hypoxia), which causes tissue damage. The body's defence system to combat the oxidative stress (e.g., anti-oxidant enzymes, free radical scavengers such as vitamin C, vitamin E, beta-carotene, reduced glutathione and minerals such as selenium, etc.) may diminish. In the present study, the antioxidant effect of selenium (Se) in reducing the hypoxia-induced oxidative stress was evaluated by exposing male albino rats to hypoxic stress in a decompression chamber. Exposure to hypoxia resulted in an increase in malondialdehyde (MDA) levels in plasma and tissues and a concurrent decrease in blood glutathione (GSH), glutathione peroxidase (GPx), plasma protein and plasma selenium content when compared with controls. Haemoglobin concentration (Hb%), red blood corpuscles (RBC) and white blood corpuscles (WBC) count were also increased in the hypoxia-exposed group. Selenium supplementation to animals reversed the trend. There was a significant decrease (P < 0.001) in MDA and subsequent increase in plasma and tissue GSH levels. Similarly the blood and tissue GPx and plasma protein also increased significantly in the Se supplemented animals compared with control animals. The Hb%, RBC and WBC counts showed no significant difference between Se-fed and control rats. These results suggest that selenium may help in reducing the lipid peroxidation during hypoxia.     

女性高血压患者绝经前后血脂和血尿酸含量分析

目的:探讨女性高血压患者绝经前后及绝经年限与血脂和血尿酸变化的关系。方法:女性高血压患者181例,分绝经前组(61例)及绝经后组(120例),测晨起空腹静脉血中甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、血尿酸(UA),进行组间比较,分析绝经年限与血脂、血尿酸水平相关性,并与103名健康对照组比较。结果:绝经前高血压组TC、LDL-C、UA显著高于对照组。绝经后高血压组TG、TC、LDL-C、UA均显著高于对照组,HDL-C则低于对照组。高血压绝经后组TG、TC、LDL-C、UA显著高于绝经前组,HDL-C低于绝经前组,TC、LDL-C与绝经年限量显著正相关。结论:女性高血压绝经后TG、TC、LDL-C、UA增高,TC、LDL-C与绝经年限量显著正相关,而HDL-C降低。     

血清脂质代谢指标水平、内脏脂肪面积与糖代谢异常合并肥胖的相关性研究

目的 探讨血清脂质代谢指标[总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)]水平、内脏脂肪面积(VFA)与糖代谢异常合并肥胖的相关性.方法 选取2019年2-10月就诊于该院内分泌科的1243例2型糖尿病患者纳入研究,根据是否合并肥胖分为糖代谢异常合并肥胖组(72...     

健康成人左心室重构与血脂比值的独立相关性北大核心CSCD

目的观察健康成人左心室重构与血脂比值的独立相关性。方法收集352名接受心脏MR(CMR)检查的健康体检者资料,测量其心脏周围脂肪组织(PAT)体积、左心室舒张末期容积(LVEDV)、左心室收缩末期容积(LVESV)、每搏输出量(SV)、射血分数(EF)和舒张末期左心室心肌质量(LVM)等,计算左心室重构指数(LVRI),即LVM/LVEDV;同时检测其血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C),计算血脂比值TC/HDL-C、TG/HDL-C及LDL-C/HDL-C;观察LVRI与血脂比值的相关性。结果LVRI与TC/HDL-C、TG/HDL-C及LDL-C/HDL-C均呈低度正相关(r=0.414、0.441、0.407,P均<0.05)。分别以TC/HDL-C、TG/HDL-C及LDL-C/HDL-C为自变量,以LVRI为因变量,以校正后的年龄、性别、心率、空腹血糖、收缩压及体表面积各因素建立多元线性回归模型1,结果显示LVRI与TC/HDL-C、TG/HDL-C及LDL-C/HDL-C均呈正相关(β=0.175、0.161、0.181,P均<0.05);在此基础上加入校正后PAT体积建立模型2,结果显示LVRI与TC/HDL-C、TG/HDL-C及LDL-C/HDL-C均呈正相关(β=0.149、0.137、0.167,P均<0.05)。结论健康成人左心室重构与血脂比值呈独立正相关,提示临床需早期防控血脂异常。     

不同程度有氧运动对代谢综合征的疗效

目的观察不同程度长期规律有氧运动对代谢综合征(metabolic syndrome,MS)的疗效。方法将106例平时缺乏运动的MS患者按随机数字表法分为对照组和研究组,每组各53例。2组均进行规律的有氧运动训练,观察组每次持续运动46~60 min,对照组每次持续运动30~45 min。比较2组治疗前及治疗6个月后体质指数(BMI)、收缩压(SBP)、舒张压(DBP)、总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白-胆固醇(HDL-C)、低密度脂蛋白-胆固醇(LDL-C)、腰围(wc)、空腹血糖(FPG)、餐后2 h血糖(2 hPG)。结果治疗后6个月,观察组BMI、SBP、DBP、TC、TG、LDL-C、wc、FPG及2 hPG较治疗前明显下降,HDL-C明显升高(均P<0.05);对照组TC、TG、LDL-C较治疗前明显下降(均P<0.05),BMI、SBP、DBP、HDL-C、wc、FPG及2 hPG较治疗前比较差异无统计学意义(均P>0.05)。与对照组比较,观察组BMI、TC、wc、FPG及2 hPG均明显下降,HDL-C明显升高(均P<0.05);2组间血压、TG、LDL-C比较差异均无统计学意义(均P>0.05)。结论控制饮食、长期规律每次持续运动46~60 min比运动30~45 min的有氧运动可以更有效地降低MS患者的BMI、血压,调节血脂,减少wc,降低FPG及2 hPG等指标。     

健康孕妇血清维生素D水平与血脂相关性研究

[目的]探讨妊娠24～28周的健康孕妇维生素D水平与血脂的相关性.[方法]选取2012年5月至2013年8月期间秦皇岛市第一医院产科门诊就诊的91例妊娠24～28周的健康孕妇为观察组,同时选取同时期于本院体检中心就诊的63例健康非妊娠女性为对照组.收集所有受试者姓名、年龄、身高、体重、孕周,测定25羟维生素D[25(OH)D]浓度、三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)等临床资料,比较两组研究对象一般资料及血清指标比较,并对血清25(OH)D水平与血脂相关性进行分析.[结果]观察组与对照组的年龄分布比较,差异无统计学意义(P＞0.05);观察组BMI明显高于对照组(P ＜0.001).观察组血清25(OH)D水平显著低于对照组,差异具有统计学意义(P＜0.001);观察组血清TG、TC、HDL-C、LDL-C水平显著高于对照组,差异均具有统计学意义(均P＜0.05).观察组25(OH)D水平与TC(r=-0.397,P=0.039＜0.05)、LDL-C(r=-0.301,P=0.041＜0.05)呈显著负相关,与年龄、BMI、TG、HDL-C均无显著相关性(均P ＞0.05).[结论]孕中期健康孕妇25(OH)D水平与血脂显著相关.     

氨氯地平阿托伐他汀钙片治疗高血压合并冠心病的临床效果

目的探讨氨氯地平阿托伐他汀钙片治疗高血压合并冠心病的临床效果。方法将我院收治的164例高血压合并冠心病患者按治疗方式不同分为对照组(82例,硝苯地平缓释片)和观察组(82例,氨氯地平阿托伐他汀钙片)。比较两组的治疗效果。结果治疗后,两组的舒张压、收缩压、TC、TG、LDL-C、CPR、IL-12、ET水平均降低,HDL-C及NO水平均升高,且观察组优于对照组(P<0.05)。结论氨氯地平阿托伐他汀钙片能改善高血压合并冠心病患者的血压、血脂水平,降低血清炎性因子水平,保护血管内皮功能。     

匹伐他汀钙治疗高胆固醇血症的有效性和安全性

目的观察匹伐他汀钙治疗高胆固醇血症的安全性和有效性。方法采用随机、双盲、阳性药物平行对照方法,将入选的高胆固醇血症48例经4周停用降血脂药物处理后,随机接受A药(匹伐他汀钙每日1 mg,A组)、B药(匹伐他汀钙每日2 mg,B组)和C药(辛伐他汀胶囊每日20 mg,C组)治疗,疗程8周。分别于治疗前、治疗4周及8周末检测计算血清低密度脂蛋白胆固醇(LDL-C)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)改变百分数,并记录血常规、尿常规、心电图、血生化检测结果及不良反应发生情况,分析降脂效果和安全性,并对3组进行比较。结果治疗前3组年龄、性别、身高、体重、收缩压、舒张压、心率以及LDL-C、TC、TG水平比较差异均无统计学意义(P>0.05);HDL-C水平比较差异有统计学意义(P<0.05),行Kruskal-Wallis检验,发现B组与C组比较差异有统计学意义(P<0.05)。与治疗前比较,治疗4周和8周末3组LDL-C、TG、TC水平均下降,差异有统计学意义(P<0.05)。3组血脂改变百分数比较差异均无统计学意义(P>0.05)。3组均未出现严重不良反应及紧急破盲事件,且无未预知的不良反应发生。结论临床使用匹伐他汀钙每日1～2 mg治疗高胆固醇血症,能有效降低LDL-C、TC、TG水平,效果确切、安全。     

n -- 3 fatty acid supplementation during pregnancy in women with allergic disease: effects on blood pressure, and maternal and fetal lipids

n--3 Fatty acids derived from fish oil reduce plasma triacylglycerols (triglycerides) and increase HDL-C (high-density lipoprotein cholesterol); however, the effect of n--3 fatty acid supplementation during pregnancy, a hyperlipidaemic state, remains unknown. We took the opportunity to investigate maternal lipid levels and blood pressure during and after pregnancy, and fetal lipid levels at birth, in a study that aimed primarily to examine the effect of fish oil supplementation during pregnancy on immune function in infants born to women with allergic disease. Eighty-three pregnant women who had allergic disease, but were otherwise healthy, completed the study. They were randomly allocated to receive fish oil or olive oil capsules, taken as 4 g/day, from 20 weeks of pregnancy until delivery. Compared with olive oil, fish oil supplementation did not alter triacylglycerols, total cholesterol, LDL-C (low-density lipoprotein cholesterol) or HDL-C during or after pregnancy. There was also no effect of fish oil on cord blood triacylglycerols, total cholesterol, LDL-C or HDL-C. Fish oil supplementation during pregnancy did not alter maternal blood pressure during or after pregnancy. The effects of fish oil on lipids and blood pressure in non-pregnant individuals appear to be lost when it is administered during pregnancy.     

胰岛素加吡格列酮联合治疗磺脲类继发性失效2型糖尿病的疗效观察

目的比较胰岛素加吡格列酮联合治疗和单用胰岛素治疗磺脲类继发性失效2型糖尿病患者的疗效.方法将磺脲类继发性失效2型糖尿病患者62例随机分为两组,治疗组32例,每日早餐及晚餐前皮下注射人胰岛素诺和灵50R及服吡格列酮15～30 mg;对照组30例,每日单用人胰岛素诺和灵50R于早餐及晚餐前皮下注射,12周后评价两组患者空服及餐后2小时血糖、糖化血红蛋白(HbA1c)、胰岛素用量、体质量指数、血压、胰岛素分泌及血脂[总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)]变化情况.结果两组患者治疗后血糖均得到良好控制,但治疗组胰岛素日需求量明显低于对照组(P<0.01);治疗组血TG较对照组下降,而HDL-C较对照组升高(P<0.05);治疗组空腹及餐后2小时胰岛素分泌较对照组减少(P<0.05).结论磺脲类继发性失效2型糖尿病患者选用胰岛素加吡格列酮联合治疗优于单用胰岛素治疗.     

非药物干预对健康体检人群高脂血症的防治效果研究

目的综合研究评价健康体检人群血脂异常非药物干预的效果。方法健康体检人员中检出高脂血症者263例,随机分为干预组132例和对照组131例,干预组给予8个月的综合性非药物干预,包括饮食控制、健康宣教、运动处方等;对照组不进行上述干预,两组观察期均为8个月。干预前后分别测定空腹血中总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、体质量指数(BMI)、空腹血糖及血尿酸(UA),血压;同时统计高脂血症相关知识知晓率、饮食和运动指标,并进行统计学分析。结果干预组TC、TG、LDL-C、LDL-C、BMI及UA各指标下降幅度与对照组比较均有统计学意义(P<0.01);且血脂下降与相关健康知识知晓水平的提高、主动性身体锻炼的增加以及食油摄入量的减少等呈明显相关(P=0.00);DBP和空腹血糖下降程度与对照组比较,差异无统计学意义(P=0.11;P=0.39)。结论非药物干预方法对高脂血症患者有一定疗效。     

高血压伴胰岛素抵抗降压调脂治疗的干预研究

目的观察硝苯地平控释片及血脂康对高血压伴胰岛素抵抗患者相关参数的影响。方法将55例高血压伴胰岛素抵抗患者随机分为单纯降压组和降压调脂组，两组均给予硝苯地平控释片，但后者加用血脂康胶囊。治疗前后行口服糖耐量实验、胰岛素释放实验、血压监测和血脂分析，观察治疗前后两组间血糖、血胰岛素、血脂、血压及胰岛素敏感指数的变化。结果用药后两组空腹血糖、空腹胰岛素及餐后2h胰岛素均无显著性下降。降压调脂组餐后2h血糖下降显著，与单纯降压组比较有显著差异。两组用药前后胰岛素敏感指数均有一定升高，以降压调脂组为著，但两组比较差异无显著性。降压调脂组治疗后总胆固醇和甘油三酯显著降低。两组降压作用相似。结论单纯降压和降压调脂都能够改善高血压患者胰岛素抵抗，以降压调脂更为明显，但短期治疗不能使胰岛素敏感性恢复正常。长期治疗可能获得预期效果。     

369例2型糖尿病患者血脂水平分析

目的：研究南京地区2型糖尿病（T2DM ）患者血脂水平变化情况及其相关影响因素。方法在空腹情况下,检测369例T2DM患者血清总胆固醇（TC）、三酰甘油（TG）、高密度脂蛋白胆固醇（HDL-C）及低密度脂蛋白胆固醇（LDL-C）等含量,然后统计分析血压、病程和家族史对T2DM患者血脂水平的影响。结果（1）T2DM 血脂水平以TG、HDL-C升高,HDL-C降低为主（P＜0．05）,且发生血脂异常的程度较T1DM重;（2）高血压Ⅱ～Ⅲ级可引起T2DM患者HDL-C显著下降,差异有统计学意义（P＜0．05）;（3）随着病程的进展,T2DM 患者 TG降低、HDL-C升高,差异有统计学意义（P＜0．05）;（4）家族史对T2DM患者血脂无影响。结论糖尿病患者存在明显的血脂异常,除受年龄、体质量指数影响外,还与血压、病程相关。     

Plasma homocyst(e)ine,folate and vitamin B(12) levels among school children in Taiwan: The Taipei Children Heart Study

OBJECTIVE: The purpose of this study is to examine the association of plasma Hcy, folate and vitamin B(12) levels on CVD risk factors among children in Taiwan. METHODS: After multistage sampling, we selected randomly 1,235 children (609 boys and 626 girls) aged 12 to 15 yr. Plasma Hcy levels was measured using ABBOTT Imx analyzer, while plasma folate and vitamin B(12) were measured using ACS: 180 automated chemiluminescence analyzer. Anthropometric, blood pressure (BP) and other biochemical CVD risk factors including serum total cholesterol (CHOL), triglyceride (TG), high density lipoprotein-cholesterol (HDL-C), apolipoprotein A1 (apoA1) and B (apoB) and lipoprotein(a) [Lp(a)]were measured using standard methods. We also calculated low density lipoprotein-cholesterol (LDL-C) and TCHR (total cholesterol to HDL-C ratio) as atherogenic indices. RESULTS: Boys were taller and heavier, having higher body mass index (BMI), systolic blood pressure (SBP) and plasma glucose levels but lower diastolic blood pressure (DBP), heart rate (HR), cholesterol, TG, HDL-C, LDL-C, TCHR, and Lp(a) levels than girls. Boys also had higher plasma Hcy and lower folate, vitamin B(12), levels than girls (for plasma Hcy, mean +/- SD, 10.50 +/- 4.13 vs. 8.95 +/- 2.61 micromol/L for boys vs. girls, respectively). Plasma Hcy levels were significantly correlated positively with body height, body weight, SBP and DBP in both genders. Body height, body weight, BMI and SBP levels were found to increase with trend among different quintile plasma Hcy subgroups on boys but not on girls. Plasma folate and vitamin B(12) were decreased significantly along with the higher quintile plasma Hcy subgroup in both genders. CONCLUSIONS: Boys had higher plasma Hcy levels than girls after adjusting for age. Furthermore, plasma Hcy levels were significantly associated with anthropometric parameters, such as body height and weight and blood pressure, but not related with lipid profiles among children in Taiwan.     

三酰甘油/高密度脂蛋白胆固醇比值对青少年肥胖人群非酒精性脂肪性肝病的预测价值

目的:探讨三酰甘油/高密度脂蛋白胆固醇比值(TG/HDL-C)对肥胖青少年非酒精性脂肪性肝病(NAFLD)的预测价值。方法:选取2012年8月至2017年8月无锡市第二人民医院内分泌科门诊或住院的肥胖青少年患者共114例,收集腰围(WC)、体质指数(BMI)、血压(BP)、氢质子磁共振波普数据(~1H-MRS)、血清谷丙转氨酶(ALT)、总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、空腹血糖(FBG)及胰岛素(INS)等临床指标,计算TG/HDL-C及稳态模型胰岛素抵抗指数(HOMA-IR)。结果:两组患者的年龄、性别、BMI、TC、腰围、舒张压、ALT和空腹血糖及胰岛素差异无统计学意义。高TG/HDL-C组患者收缩压和肝脏三酰甘油含量均显著高于低TG/HDL-C组(P0.05)。多因素Logistic回归分析提示:TG/HDL-C是青少年肥胖人群患NAFLD的独立危险因素(OR=5.78,95%CI 2.23～14.97)。TG/HDL-C预测青少年肥胖人群患NAFLD的ROC曲线下面积为0.73,以2.64为诊断界点时,灵敏度为72.5%,特异度为71.6%。结论:TG/HDL-C有助于预测肥胖青少年人群NAFLD的患病风险。     

玉米黄质对高脂诱发鹌鹑模型血管脂质过氧化损伤的保护作用

背景:大多数人工合成的抗氧化药物长期应用均有一定的毒副作用,玉米黄质作为天然药物有其独特的生理效应。目的:观察玉米黄质对高脂诱发血管脂质过氧化损伤效应鹌鹑模型影响的量效关系。方法:建立鹌鹑高脂模型,利用玉米黄质按30,60mg/kg灌胃7周后,取空腹血测定血清总胆固醇、三酰甘油、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇及丙二醛水平、血清超氧化物歧化酶、谷胱甘肽过氧化物酶及诱导型一氧化氮合酶活性;检测各组动物肝脏组织中总胆固醇、三酰甘油水平;并采用苏木精-伊红染色观察主动脉血管病理变化。结果与结论:与高脂模型组相比,玉米黄质组血清三酰甘油、总胆固醇和低密度脂蛋白胆固醇浓度明显降低,而血清高密度脂蛋白胆固醇浓度明显增高;肝脏总三酰甘油及总胆固醇的合成也受到强烈抑制;玉米黄质组血清超氧化物歧化酶活性、谷胱甘肽过氧化物酶活性明显提高,而诱导型一氧化氮合酶活性下降、丙二醛水平减少。说明玉米黄质具有良好的降脂功效,对高脂诱导的血管脂质过氧化损伤有明显的抑制作用,且高剂量较低剂量作用效果更为理想。