支气管哮喘病人T淋巴细胞亚群测定

本文应用抗人T淋巴细胞亚群单克隆抗体测定了90例哮喘儿童及32例正常对照儿童外周血T淋巴细胞亚群,结果表明哮喘病人无明显成熟T淋巴细胞、T_H亚群和T_s亚群异常。提示病人B淋巴细胞过度产生IgE可能是在更精细的水平上受T淋巴细胞所调节。     

支气管哮喘患儿外周血B细胞和T细胞及其亚群的研究

目的 :探讨了支气管哮喘患儿外周血B细胞和T淋巴细胞及其亚群的变化。方法 :应用单克隆技术测定了 38例支气管哮喘患儿外周血B细胞和T淋巴细胞亚群的水平并以 30名正常健康人作比较。结果 :支气管哮喘患儿外周血B细胞数显著地高于正常人组 (P <0 .0 1 )CD3 、CD4、CD4/CD8显著地低于正常人 (P <0 .0 1 )。结论 :支气管哮喘是一种自身免疫调节异常的疾病     

人类T_H细胞亚群与支气管哮喘

本文列举了人类存在Ｔ辅助细胞（Ｔ＿Ｈ）功能性亚群（Ｔ＿Ｈ和Ｔ＿Ｈ２）的证据；叙述调节Ｔ＿Ｈ细胞亚群分化的因素，尤其是Ｔ＿Ｈ１和Ｔ＿Ｈ２的相互制约作用；并讨论了Ｔ＿Ｈ２细胞在支气管哮喘慢性气管炎症形成中的作用，为防治哮喘提供新的思路。     

支气管哮喘患儿T淋巴细胞亚群和sIL-2R的检测

支气管哮喘是儿童常见棘手疾病之一 ,近年来发病率有升高趋势 ,其发病机理尚未完全清楚。我们对 38例急性发作期 ,2 6例恢复期哮喘患儿Ｔ淋巴细胞亚群和ｓＩＬ 2Ｒ进行了检测 ,以探讨Ｔ淋巴细胞在哮喘发生发展中的作用。1　材料与方法1 1　研究对象　根据儿童哮喘诊断标准[1] ,     

支气管哮喘患者外周血T细胞亚群的表达及临床分析

支气管哮喘是以气道高反应性和可逆性气流受阻为特征的慢性气道炎症性疾病。很多细胞参与该炎症过程,其病理基础是多种炎症性细胞（肥大细胞、嗜酸性粒细胞、巨噬细胞、中性粒细胞、淋巴细胞等）及自细胞介素等细胞因子和黏附分子的相互调控下释放炎症介质引起支气管平滑肌收缩、微血管渗漏,黏液腺分泌增加以及直接损伤气道上皮甚至剥脱,并导致气道高反应性发生的病理基础,气道高反应性是支气管哮喘的特征。其中T淋巴细胞及其分泌的细胞因子在哮喘的发病过程中发挥着重要的免疫调节作用。     

遗传性支气管哮喘家系过敏原皮试的特点

通过对一常染色体显性遗传的支气管哮喘家系成员１３４例过敏原皮肤试验观察，发现这一家系中，与上代有血缘关系的家系成员中，哮喘患者对几种常见的过敏原的阳性反应率反而低于未发病者的反常现象，并观察到有血缘关系者对过敏原蟑螂阳性反应率显著高于非血缘这有系成员，指出这些观察结果在分析这一家系哮喘遗传和发病方面的意义。     

支气管哮喘患儿血清cTnⅠ水平与外周血B细胞和T细胞及其亚群的分析

支气管哮喘为儿科的常见病、多发病,众多的研究表明,该病是由于细胞免疫功能障碍引起的一种免疫紊乱性疾病[1]。心肌肌钙蛋白I（TnI）存在于心肌收缩蛋白细丝上,在心肌收缩和舒张的过程中起十分重要的作用[2]。我们对支气管哮喘患儿血清cTnI水平与外周血B细胞和T淋巴细胞亚群进行检测，现将结果报告如下。     

加味小青龙汤对支气管哮喘患者肺功能及T淋巴细胞亚群水平的影响

目的:探究加味小青龙汤对支气管哮喘(Bronchial asthma,BA)患者肺功能及T淋巴细胞亚群水平的影响.方法:选取2018年12月至2020年7月我院BA患者104例,根据治疗方案不同分组,各52例.对照组给予西药治疗,观察组于对照组基础上加用加味小青龙汤.对比两组治疗前后哮喘控制测试(Asthma control test,ACT)评分、肺功能指标、T淋巴细胞亚群、氧化-抗氧化指标[谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)、超氧化物歧化酶水平(Superoxide dismutase,SOD)、8-异前列腺素(8-iso prostaglandin,8-iso-PG)、丙二醛(Malondialdehyde,MDA)].结果:治疗后观察组ACT评分高于对照组(P<0.05);治疗后观察组肺功能指标、T淋巴细胞亚群,GSH-Px、SOD水平高于对照组(P<0.05),血清8-iso-PG、MDA水平低于对照组(P<0.05).结论:加味小青龙汤联合治疗BA,可改善患者氧化-抗氧化平衡,调节T淋巴细胞亚群水平,改善临床症状及肺功能.     

哮喘患者支气管肺泡灌洗液中细胞分类及其T细胞亚群的改变

目的和方法：采用间接免疫荧光法和支气管肺泡灌洗液（ｂｒｏｎｃｈｉａｌａｌｖｅｏｌａｒｌａｖａｇｅｆｌｕｉｄ，ＢＡＬＦ）技术观察１２例过敏性哮喘患者和２３例健康人外周血和ＢＡＬＦ中的Ｔ淋巴细胞亚群变化：结果：与健康对照组比较，过敏性哮喘患者外周血的Ｔ淋巴细胞亚群无明显改变，但其ＢＡＬＦ中的ＣＤ４＋细胞显著增高（５４．９７±４１４）％ｖｓ（７９．７１±９．６３）％，Ｐ＜００５；ＣＤ４＋与ＣＤ８＋细胞的比值也显著增高（１６４±０．３２ｖｓ２．３２±０．８３，Ｐ＜００５）。此外，过敏性哮喘患者ＢＡＬＦ中肥大细胞和嗜酸细胞百分比（００９±０．０４）％和（３６２±１．０６）％明显高于健康对照组（００２±０．０１）％和（０．３９±０．３０）％，Ｐ＜００５和Ｐ＜００１。结论：ＣＤ４＋细胞在哮喘的气道炎症中发挥重要作用。     

肺癌患者外周血T淋巴细胞亚群变化特点及临床意义

目的:探讨肺癌患者外周血T淋巴细胞变化及其临床意义。方法:采用流式细胞术检测法检测56例肺癌患者(其中20例早中期肺癌、36例晚期肺癌);18例健康人群外周血单个核细胞(Peripheral blood mononuclear cells,PBMC)中总T细胞(CD3+)、Th细胞(CD3+CD4+)、Tc细胞(CD3+CD8+)、NKT细胞(CD3+CD16+CD56+)、NK细胞(CD3-CD16+CD56+)、调节性T细胞(Treg,CD4+CD25+Foxp3+)占CD4+T细胞比例和CD3+γδT细胞比例。结果:56例肺癌患者总T细胞(CD3+)比例明显低于健康组(61.41%±7.88%vs71.63%±5.59%,P0.001),肺癌患者Tc细胞比例明显低于健康组(23.58%±7.18%vs28.44%±5.20%,P0.05),肺癌患者Treg比例明显高于健康组(6.20%±1.63%vs3.65%±2.00%,P0.001);肿瘤组外周血CD3+γδT细胞比例显著低于健康组(3.35%±1.41%vs5.53%±1.87%,P0.01)。但肺癌患者晚期组与肺癌早中期组比较外周血CD3+γδT细胞比例显著增高(3.70%±1.89%vs2.64%±1.41%,P0.05),肺癌患者晚期组与早中期组比较外周血Treg细胞比较显著增高(6.78%±2.64%vs5.06%±1.22%,P0.05)。肺癌患者外周血中NK细胞低于健康组(15.02%±7.61%vs18.74%±6.39%,P0.05),而Th细胞和NKT细胞都有所降低,但差异尚不具统计学意义(P0.05)。结论:肺癌患者总T细胞、Th细胞、Tc细胞和NK细胞都有所降低,Treg细胞有所上升,提示肺癌患者处于免疫抑制状态。Treg细胞和CD3+γδT细胞与肺癌的临床病程具有一定的关联性。     

Depletion of circulating T lymphocytes in pregnancy.

Changes were assessed in lymphocyte sub-populations in various stages of human pregnancy. The percentage and absolute number of E-RFC decreased during pregnancy. There was a concomitant rise in the percentages of EAC-RFC and cells bearing SmIg with little change in their absolute numbers. EAC-RFC continued to rise post-natally.     

Omalizumab therapy is associated with reduced circulating basophil populations in asthmatic children

Basophils have been implicated in promoting the early development of T_H2 cell responses in some murine models of T_H2 cytokine‐associated inflammation. However, the specific role of basophils in allergic asthma remains an active area of research. Recent studies in animal models and human subjects suggest that IgE may regulate the homeostasis of human basophil populations. Here, we examine basophil populations in children with severe asthma before and during therapy with the IgE‐directed monoclonal antibody omalizumab. Omalizumab therapy was associated with a significant reduction in circulating basophil numbers, a finding that was concurrent with improved clinical outcomes. The observation that circulating basophils are reduced following omalizumab therapy supports a mechanistic link between IgE levels and circulating basophil populations, and may provide new insights into one mechanism by which omalizumab improves asthma symptoms.     

Proteomic analysis of peripheral T lymphocytes, suitable circulating biosensors of strictly related diseases

T lymphocytes and/or their subpopulations from peripheral blood may represent molecular sensors to be used for the evaluation of gene expression modification in physiological and pathological conditions, providing a unique and easily available biological model for integrated studies of gene expression in humans. In this study, a proteomic approach was applied to evaluate the association between changes in T cell protein expression patterns and specific diseased conditions. In particular, two hyperandrogenic syndromes were studied, sharing many clinical and biochemical signs: polycystic ovary syndrome (PCOS) and congenital adrenal hyperplasia (CAH). Comparison of proteomic maps of T lymphocytes derived from patients affected by PCOS or CAH with those derived from healthy subjects showed that 14 proteins are expressed differentially in both PCOS and CAH, 15 exclusively in PCOS and 35 exclusively in CAH. Seventeen of these proteins have been identified by mass spectrometry analysis. Furthermore, proteomic data mining by hierarchical clustering was performed, highlighting T lymphocytes competence as a living biosensor system.     

致敏树突状细胞活化细胞毒性T细胞抗肿瘤作用的研究

目的：研究树突状细胞（DCs）激活的细胞毒性T细胞的抗肿瘤及预防肿瘤发生的作用。 方法： 细胞因子诱生人PBMC未成熟DCs，加入肿瘤细胞抗原提取物致敏DCs产生成熟DCs；通过细胞形态、表面标记鉴定成熟DCs，MTT法测成熟DCs活化的细胞毒性T细胞(CTL)的体外杀伤活性；裸鼠体内注射活化CTL观察其抑制移植瘤生长及发生的作用。 结果： 经过7 d培养，获得大量形态典型、具有强烈刺激增殖能力、高表达CD80(63.5%)、CD83（67.6%）和CD3/ HLA-DR(83.2%)的DCs。其活化的CTL在20∶1效靶比时对抗原来源细胞株自身的杀伤率达75%以上，对同系细胞株的杀伤活性为35%-45%，对其它种系肿瘤细胞仅有微弱杀伤力（P<0.01）。CTL对裸鼠结肠癌HT-29移植瘤有特异性的生长抑制和预防生成作用（P<0.05）。CTL治疗组肿瘤组织中PCNA表达水平显著低于对照组（P<0.05）。 结论： 肿瘤细胞抗原活化的DC诱导CTL对肿瘤有特异性的杀伤作用，体内应用可特异性抑制移植结肠癌的生长或预防小鼠结肠癌移植瘤的发生。     

Recruitment of effector lymphocytes by initiator lymphocytes. In vivo migration of in vitro sensitized initiator T lymphocytes

We previously found that mouse T lymphocytes sensitized in vitro against allo- or syngeneic fibroblasts, upon injection into syngeneic recipients, do not themselves differentiate into effector cells, but recruit effector T lymphocytes within the draining lymph nodes. Asaresult of sensitization, these initiator lymphocytes acquire a trypsin-sensitive membrane property which is necessary for recruitment. We now report studies on the in vivo migratory behavior of initiator lymphocytes following sensitization. We injected ⁵¹Cr-labeled initiator lymphocytes into recipient footpads and found significantly increased migration of sensitized cells to the draining popliteal lymph node (PLN) during the first day. By amputation ofthe foot at various times, we showed that migration during the first 12—24 hours was critical for subsequent recruitment. Trypsin treatment of initiator lymphocytes abolished this accelerated migration. Lymphocytes triggered nonspecifically by Con A migrated to the PLN like antigen-sensitized cells. We also compared the migration of injected lymphocytes from the footpad to the PLN in graftversus-host and host-versus-graft reactions, and found these reactions to differ both from each other and from recruitment in terms of lymphocyte migration. These findings are discussed in terms of the physiology of the cell-mediated immune response and the notion of peripheral sensitization.     

Recruitment of effector lymphocytes by initiator lymphocytes. In vivo migration of in vitro sensitized initiator T lymphocytes.

We previously found that mouse T lymphocytes sensitized in vitro against allo- or syngeneic fibroblasts, upon injection into syngeneic recipients, do not themselves differentiate into effector cells, but recruit effector T lymphocytes within the draining lymph nodes. As a result of sensitization, these initiator lymphocytes acquire a trypsin-sensitive membrane property which is necessary for recruitment. We now report studies on the in vivo migratory behavior of initiator lymphocytes following sensitization. We injected 51Cr-labeled initiator lymphocytes into recipient footpads and found significantly increased migration of sensitized cells to the draining popliteal lymph node (PLN) during the first day. By amputation of the foot at various times, we showed that migration during the first 12-24 hours was critical for subsequent recruitment. Trypsin treatment of initiator lymphocytes abolished this accelerated migration. Lymphocytes triggered nonspecifically by Con A migrated to the PLN like antigen-sensitized cells. We also compared the migration of injected lymphocytes from the footpad to the PLN in graft-versus-host and host-versus-graft reactions, and found these reactions to differ both from each other and from recruitment in terms of lymphocyte migration. These findings are discussed in terms of the physiology of the cell-mediated immune response and the notion of peripheral sensitization.     

Loss of circulating T lymphocytes with normal levels of B and ''null'' lymphocytes in Thai adults with malaria.

Peripheral blood mononuclear cells from forty-nine Thai adults infected with either Plasmodium falciparum or Plasmodium vivax were examined in order to determine the percentage of T, B, and Fc-receptor-bearing cells present. In comparison to healthy controls, both the percentage and concentration of peripheral T cells were decreased in the malaria-infected individuals as assessed by formation of rosettes with sheep red blood cells. The percentage of peripheral B cells was increased but their concentration was unchanged, as assessed by two techniques: the presence of surface immunoglobulin and the presence of a complement receptor. Both the percentage and concentration of lymphocytes bearing Fc receptors were unchanged in infected individuals. Finally, calculation of the changes in 'null' cells (defined either as non-T, non-B lymphocytes or as non-T, non-B, non Fc-receptor-bearing lymphocytes) revealed an increase in the 'null' cell percentage but a decrease in the absolute number of 'null' cells. These data indicate that in adult Thai patients naturally infected with malaria, there is a real loss of circulating T lymphocytes with no real change in B, Fc-receptor-bearing, or 'null' lymphocytes.     

Predictable clinical disorders related to serum and saliva Ig-levels and the number of circulating T cells in asthmatic children

An examination was made of 221 children with bronchial asthma, who were divided into six groups according to serum and saliva Ig levels and the number of circulating T cells. Absence or small amounts of IgA and low or low-normal numbers of T cells were associated with (1) atopic dermatitis, (2) hypersensitivity to house dust mite and animal danders, (3) previous hospital admissions due to respiratory tract infections with pathogenic bacteria and (4) a high family incidence of allergic diseases. In a group of patients with IgA deficiency and elevated serum and saliva IgM, respiratory tract infections were not common, and furthermore, in another group of IgA-deficient patients with normal numbers of circulating T cells, atopic dermatitis was rare. In the latter patients, allergic rhinitis occurred very frequently, and in that respect they resembled a group of patients with combined high IgM/high IgE levels. Another group of asthmatic children with normal Ig levels represented an intermediate type of patient with regard to hypersensitivity to different allergens and family incidence of allergy on the one hand, and the occurrence of atopic dermatitis and allergic rhinitis on the other. Investigations on Ig levels and circulating T cells in asthmatic children may provide important clues into disease classification and mechanisms of such patients.     

Factors associated with increased respiratory symptoms among asthmatic children in Singapore

Asthma is a common cause of childhood morbidity. The objective of the present study was to evaluate the factors associated with increased asthma morbidity among asthmatic children in Singapore. A cohort of primary school children (n = 6,404, aged 6-13 years) were evaluated using the American Thoracic Society and the Division of Lung Diseases of the National Heart, Lung and Blood Institute, USA (ATS-DLD) respiratory questionnaire. A total of 2,222 of 6,404 children (34.8%) was found to have reported symptoms of wheezing. Of these, 899/2,222 (40.5%) reported symptoms of "increased asthma morbidity". This was associated with the younger age group, male sex and higher socio-economic status. In addition, concurrent or past allergies were strongly associated with increased asthma morbidity, while premature birth and a history of prior childhood respiratory illnesses and Infections were predictive of greater asthma morbidity. No association was found between increased morbidity and presence of domestic pets, parental smoking, childcare attendance, and the season of birth.     

T lymphocytes in children with allergic respiratory disease.

The role of T lymphocytes in atopic disease is of considerable importance because animal studies indicate that cells of this lymphoid series may influence reaginic antibody response. T lymphocyte subpopulations were studied in a group of 76 children with allergic respiratory disease. There was no statistical difference between atopic children with asthma and those with allergic rhinitis as compared with an age-matched control population of 20 non-atopic children in terms of levels of active T lymphocytes or total T lymphocytes. The results of this study do not support the concept of a T cell immunodeficiency in children with allergic respiratory disease.