CEA、CA199、CA125对老年肺癌淋巴结转移的诊断价值

目的探讨癌胚抗原(CEA)、糖类抗原(CA)125、CA199在老年患者良、恶性肺结节血清中的表达及对老年患者发生淋巴结转移的诊断价值,并探讨其与肿瘤病理分型的相关性。方法选取住院老年肺癌(恶性肺结节组)患者236例,同期确诊的老年良性肺结节(良性肺结节组)患者97例。全自动电化学发光分析仪测定两组血清CEA、CA125、CA199水平。分析CEA、CA125、CA199水平与恶性肺结节组病理分型的关系及对淋巴结转移的诊断价值。结果恶性肺结节组中有淋巴结转移组CEA和CA125水平均显著高于无淋巴结转移组(均P<0.05),鳞癌和小细胞癌患者血清CA199水平差异显著(P<0.05),各病理分型患者和良性肺结节组血清CEA水平相比差异均有统计学意义(P<0.05)。结论老年恶性肺结节患者中CEA、CA125、CA199水平均高表达,CEA、CA125水平对老年患者淋巴结转移有一定预测价值。     

血清miR-320a、MACC1对结肠癌术后复发转移的预测价值

目的 探讨血清微小RNA-320a(miR-320a)、结肠癌转移关联基因1(MACC1)对结肠癌术后复发转移的预测价值.方法 选取102例结肠癌患者为结肠癌组,63例结肠良性病变患者为对照组.qRT-PCR测定血清miR-320a表达,ELISA法测定血清MACC1,Pearson相关法分析结肠癌患者血清miR-32...     

癌胚抗原、糖类抗原125、糖类抗原50联合检测对老年胃癌的诊断价值

<正>早期诊断与根治性治疗是胃癌取得良好预后的极关键途径。而胃癌的诊断又要得益于临床检验的准确性,在临床检验过程中,肿瘤标志物是非常重要的一个切入点〔1,2〕。目前尚未找到具有高敏感性及特异性的单一胃癌肿瘤标志物。只有采用联合检测手段方可提高胃癌诊断的敏感性与特异性〔3〕。本研究探讨3种肿瘤标志物癌胚抗原(CEA)、糖类抗原125(CA125)、糖类抗原50(CA50)联合检测诊断胃癌的价值。     

血清CEA、CA199、CA125、、CA50单独及联合检测诊断胆囊癌的价值

目的 探讨血清CEA、CA199、CA125、CA50水平单项检测与联合检测在胆囊癌诊断中的应用价值.方法 以80例胆囊癌患者为胆囊癌组,90例胆囊良性疾病患者为对照组.比较两组患者血清CEA、CA199、CA125、CA50.分别以CEA＞ 10 ng/mL、CA199＞ 37 U/mL、CA125＞ 35 U/mL、CA50＞ 20 U/mL为阳性结果,计算两组上述指标单独及联合检测阳性结果出现率(阳性率).结果 胆囊癌组血清CEA、CA199、CA125、CA50水平明显高于对照组,P均＜0.05.胆囊癌组单项指标检测阳性率:CEA为25％,CA199为81.25％,CA125为46.25％,CA50为76.25％;联合检测两两组合中阳性率最高者为CA199+CA50(93.75％),三项联合阳性率最高者为CA199+CA125+CA50(96.25％),四项联合检测的阳性率为96.25％,为所有组合中最高.结论 血清CEA、CA199、CA125、CA50水平单项检测诊断胆囊癌检出率不高,联合检测有助于提高胆囊癌检出率.     

乳腺癌患者血清糖类抗原153、癌胚抗原、铁蛋白及降钙素水平变化的价值

目的探讨乳腺癌患者血清糖类抗原(CA) 153、癌胚抗原(CEA)、铁蛋白(SF)及降钙素(PCT)水平变化的临床意义。方法乳腺癌患者293例为观察组,乳腺良性肿块100例作为对照组。所有研究对象于入院后第2天清晨空腹抽取3 ml外周静脉血,采用化学发光免疫分析法测定血清CA153、CEA和SF含量,采用散射比浊法测定PCT含量。结果观察组血清CA153、CEA、SF和PCT含量显著高于对照组,差异有统计学意义(P<0. 05)。Ⅲ～Ⅳ期血清CA153、CEA、SF和PCT含量显著高于Ⅰ～Ⅱ期,有统计学意义(P<0. 05)。CA153+CEA+SF+PCT联合诊断的灵敏度和特异度高于各单项。结论乳腺癌患者血清CA153、CEA、SF和PCT高表达,联合诊断灵敏度和特异度高。     

血清miR-335-5p、CEA、CA19-9水平对结肠癌腹腔镜术后复发转移的预测价值

目的 探讨血清微小RNA-335-5p(miR-335-5p)、癌胚抗原(CEA)、糖类抗原19-9(CA19-9)水平对结肠癌腹腔镜术后复发转移的预测价值.方法 选择结肠癌患者89例(观察组)、结肠良性病变患者61例(对照组),采集两组术前外周静脉血,检测血清miR-335-5p、CEA、CA19-9,分析血清miR...     

胸液糖类抗原—50癌胚抗原铁蛋白联合检测的临床价值

胸液糖类抗原－５０癌胚抗原铁蛋白联合检测的临床价值刘霞秋陈余清观察了７５例良恶性胸液患者血清和胸液中糖类抗原－５０（ＣＡ－５０）水平，并联合检测了癌胚抗原（ＣＥＡ）和铁蛋白，以探讨其在良恶性胸液鉴别诊断中的临床价值。对象和方法：７５例胸液患者中，良性...     

卵巢上皮恶性肿瘤中糖类抗原125和癌胚抗原的临床意义

目的探讨卵巢上皮恶性肿瘤中糖类抗原125(CA125)和癌胚抗原(CEA)的临床意义。方法选择该院2011年1月至2012年12月收治的42例卵巢上皮恶性肿瘤患者为观察组,同期收治的40例卵巢良性肿瘤患者为对照组,比较两组患者血浆CA125和CEA水平,分析两组患者血浆CA125、CEA及联合诊断阳性率,观察不同病理类型卵巢上皮恶性肿瘤患者血浆CA125、CEA及联合诊断阳性率。结果观察组血浆CA125〔(539.17±723.58)U/ml〕、血浆CEA〔(9.33±6.90)ng/ml〕水平显著高于对照组〔(11.82±10.03)U/ml、(2.38±1.74)ng/ml〕(P<0.0001)。观察组血浆CA125诊断阳性率为73.81%,血浆CEA诊断阳性率为38.01%,联合诊断阳性率为92.86%;对照组为7.50%,0.75%;观察组阳性率显著高于对照组(P<0.0001)。不同病理类型卵巢上皮恶性肿瘤患者血浆CA125、CEA及联合诊断阳性率比较无统计学意义(P>0.05)。结论血浆CA125和CEA可有效反映卵巢上皮肿瘤病变程度,对于卵巢上皮恶性肿瘤的诊断、监控及预后具有重要临床意义。     

糖类抗原19-9联合癌胚抗原检测诊断老年胃癌的价值

目的探讨糖类抗原(CA)19-9联合癌胚抗原(CEA)检测老年胃癌的诊断价值。方法老年胃癌患者72例作为病例组,同时随机选取非胃癌患者72例作为对照组,应用电化学发光免疫分析法分析两组CA19-9及CEA水平,分别计算CA19-9、CEA单独及联合诊断胃癌的敏感性、特异性及准确性。结果胃癌组CA19-9及CEA的平均水平分别为(92.35±36.71)U/L、(17.15±7.14)μg/L,显著高于对照组〔(36.65±18.26)U/L及(3.58±2.64)μg/L〕(P0.05)。随着胃癌临床分期的加重、肿瘤分化级别的减低,CA19-9、CEA的水平进行性升高(P0.05)。CA19-9单独检测胃癌的灵敏度、特异度分别为45.8%、93.1%,而CEA单独检测的灵敏度、特异度分别为50.0%、90.3%,二者联合检测时为84.7%、86.1%,准确性为85.4%。结论血清CEA、CA199联合检测有助于提高胃癌诊断的敏感性及准确性,对胃癌的早期诊断有重要价值。     

癌胚抗原、糖类抗原211、前列腺特异抗原在老年人肿瘤筛查中的意义

<正>老年人是肿瘤的高发人群,其肿瘤发病特点为发病率高,转移率高,死亡率高,且早期诊断率低,多数患者就诊时已处于进展期,肿瘤复发和转移是治疗失败的主要原因~[1]。多数老年患者患病后初次就诊已经伴有广泛的淋巴结转移或者远端的转移,已处于该病的晚期,失去手术根治的机会,耽误了治疗的最佳时机,早期诊断对疾病的及时发现及治疗具有重要的临床意义~[1-3]。大样本多中心的研究发现很多基因参与了老年人肿瘤发生。寻找老年人肿瘤发生、发展的     

Role of SPan-1 antigen in adhesion of human colon cancer cells to vascular endothelium

Recently E-selectin (ELAM-1, endothelial leukocyte adhesion molecule-1) was shown to recognize not only sialyl Lewis X but also sialyl Lewis A, and these carbohydrate antigens may be involved in the process of the adhesion between cancer cells and endothelial cells in cancer metastasis. To investigate the contribution of sialylated carbohydrate antigen, SPan-1, and sialic acid to the adhesion of human colon cancer cells to endothelial cells, adhesion assay using HUVECs (human umbilical vein endothelial cells) was performed. The adhesion was significantly inhibited by pretreatment with anti-E-selectin antibody, indicating that this adhesion was thought to be mediated by E-selectin. When these cancer cells were pretreated with SPan-1 antibody, the adhesion was significantly inhibited in a concentration-dependent manner. The adhesion was also inhibited by pretreatment with neuraminidase. These findings suggest that the SPan-1 antigen plays a significant role in the adhesion of human colon cancer cells to endothelial cells, and sialylation of the terminal structure of carbohydrate antigens is important in this adhesion.Part of this study was presented at the Second Osaka International Symposium on Gastroenterology held in Osaka, Japan on November 22–23, 1993.     

Preoperative serum carcinoembryonic antigen and carbohydrate antigen 19-9 levels for the evaluation of curability and resectability in patients with pancreatic adenocarcinoma

Background Purpose

Although carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9) are the most studied serum tumor markers that have been evaluated for diagnosis and prognosis in patients with pancreatic cancer, little is known of the value of these markers for the prediction of curability and resectability.

Methods

We retrospectively reviewed preoperative serum levels of CEA and CA 19-9 in 244 consecutive patients with pancreatic operations.

Results

Although 159 pancreatic operations seemed “resectable”, 93 of them were judged curative (R0) and the other 66 turned out to be noncurative (R1/2). The remaining 85 failed resection because of unexpected metastasis or locally advanced disease (LD), which was unresectable compared with levels in those patients without liver metastasis or LD. CEA levels were significantly higher in patients with liver metastasis and LD, while CA 19-9 levels were correlated with liver and peritoneal metastases. When both markers were negative, curative (R0) and respectable (R0 + R1/2) operation were performed in 70% and 85% of patients, respectively. Logistic regression analysis indicated that under conditions where both CEA and CA 19-9 were negative, the odds ratios for curative and respectable operations were 4.43 and 3.58, respectively.

Conclusions

Our data suggest that combined preoperative CEA and CA 19-9 levels are suitable for assessing expected curability and resectability in patients with pancreatic cancer.

     

热疗对食管癌患者的辅助治疗作用及其对血清糖类抗原199及血管内皮生长因子的影响

目的探讨热疗对食管癌患者的辅助治疗作用及其对血清糖类抗原199(CA199)及血管内皮生长因子(VEGF)的影响。方法选取2012年9月至2016年9月宝鸡市人民医院胸外科收治的食管癌患者100例,依据随机数字表法将患者分为热疗组和对照组,每组50例。对照组给予多西他赛+奈达铂化疗治疗及常规放射疗法,热疗组在对照组方案基础上给予热疗辅助治疗。比较2组患者血清CA199、VEGF水平及治疗疗效、不良反应及生存情况。采用SPSS 22.0统计软件对数据进行分析。根据数据类型,组间比较采用t检验、χ2检验或秩和Z检验,组内比较采用自身配对t检验。对生存预后采用KaplanMeier曲线分析。结果热疗组患者治疗有效率明显高于对照组,差异有统计学意义[90.00%(45/50)vs 74.00%(37/50),P=0.04]。2组患者治疗后血清CA199及VEGF水平均明显低于治疗前,且热疗组患者治疗后血清CA199[(50.42±5.54)vs(76.45±7.72)U/ml]及VEGF[(137.2±15.5)vs(203.5±24.0)pg/ml]水平明显低于对照组,差异有统计学意义(P0.01)。2组患者不良反应发生率比较,差异无统计学意义(P=0.46);随访2年,热疗组治疗生存率明显高于对照组,差异有统计学意义[60.00%(30/50)vs 40.00%(20/50),P=0.03]。结论在传统化疗及放射治疗基础上,热疗可有效提高食管癌患者的治疗疗效,改善生存情况,具有良好的安全性,值得临床进一步推广。     

支气管肺泡灌洗液、血清中铁蛋白、CA-199、CEA对肺癌的诊断价值

目的探讨支气管肺泡灌洗液（BALF）、血清中铁蛋白（SF）、糖链抗原199（CA-199）和癌胚抗原（CEA）测定对肺癌的诊断价值。方法采用免疫放射分析法（IRMA）测定60例肺癌患者和68例肺良性疾病患者BALF和血清中SF、CA-199、CEA的水平。结果肺癌组BALF和血清中SF、CA-199、CEA明显高于肺良性疾病组,有非常显著性差异（P〈0．0001）,肺癌组BALF的SF、CA-199、CEA水平高于血清,有显著性差异（P〈0．001）,肺癌组3项联合检测的敏感性及准确性明显高于单项检测（P〈0．05）。结论联合检测BALF及血清中的SF、CA-199、CEA对于鉴别肺部良、恶性疾病有较好的临床价值,对肺癌的早期诊断提供良好手段。     

区域化疗对结肠癌术后复发及肝转移的影响

目的　探讨不同化疗方法对结肠癌术后局部复发和肝转移的影响．方法　Ｄｕｋｅｓ＇ Ｃ１ ～Ｃ３ 期结肠癌患者４９ 例,术后随机分成３ 组,以区域化疗（ ｎ ＝ ２６） 、全身化疗（ ｎ ＝ １２） 及口服化疗药（ ｎ ＝ １１）３ 种方法进行治疗．结果　区域化疗组术后复发和肝转移明显低（５０ ％ ｖｓ ９２ ％ 及９１ ％ ） 于全身化疗及口服化疗药组．结论　结肠癌术中、术后以ＦＭＣ 方案定期化疗,对减少复发和肝转移将起到积极作用．     

Sputum carcinoembryonic antigen, neuron-specific enolase and cytokeratin fragment 19 levels in lung cancer diagnosis

OBJECTIVE: The aim of the present study was to examine the impact of sputum carcinoembryonic antigen (CEA), neuron-specific enolase (NSE) and cytokeratin fragment 19 (CYFRA 21-1) levels in lung cancer diagnosis and to compare the diagnostic usefulness of sputum assays with that of serum assays. METHODOLOGY: Forty-seven patients with lung cancer and 62 with benign lung disease were studied. Tumour marker levels in sputum (sp.) and serum (ser) were measured by immunoradiometric assays. RESULTS: Sputum and serum tumour marker levels were significantly higher in lung cancer than in benign disease. When the specificity was 95%, the sensitivity was 57%, 43%, 36%, 30%, 28% and 19%, for spCEA, serCYFRA 21-1, spCYFRA 21-1, serCEA, serNSE, and spNSE, respectively. Bayesian analysis showed that the best predictive values correspond to spCEA and serCYFRA 21-1. The maximum overall gain was obtained in pretest probability of 0.35 for both spCEA and serCYFRA 21-1, with predictive values of 84% and 80% for spCEA and serCYFRA 21-1, respectively. CONCLUSION: Sputum tumour marker levels were no more useful than the serum levels in lung cancer diagnosis. SpCEA offered the best predictive values but these were still not sufficiently satisfactory for spCEA to be proposed for routine use.     

Clinicopathological characteristics and prognosis of colon cancer with lung metastasis without liver metastasis: A large population-based analysis

Distant metastasis explains the high mortality rate of colon cancer, in which lung metastasis without liver metastasis (LuM) is a rare subtype. This study is aimed to identify risk factors of LuM and LLM (lung metastasis with liver metastasis) from colon cancer, and to analyze the prognosis of patients with LuM by creating a nomogram. Patients’ information were obtained from the Surveillance, Epidemiology, and End Results (SEER) database. Multivariable logistic regression analysis was used to determine the risk factors for LuM and LLM. Prognostic factors for cancer-specific survival (CSS) and overall survival (OS) were identified by multivariate Cox proportional hazards regression and nomogram models were established to predict CSS and OS. Multivariate logistic regression analysis showed that blacks, splenic flexure of colon tumor, tumor size >5 cm, T4, N3, and higher lymph node positive rate were associated with the occurrence of LuM. Meanwhile, age >65 years old, female, splenic flexure of colon, higher lymph node positive rate, and brain metastasis were independent risk factors for CSS. The C-index of the prediction model for CSS was 0.719 (95% CI: 0.691–0.747). In addition, age, primary site, tumor size, differentiation grade, N stage, and bone metastasis were significantly different between LuM and LLM. The nomograms we created were effective in predicting the survival of individuals. Furthermore, patients with LuM and LLM from colon cancer might require different follow-up intervals and examinations.     

KAI1基因的表达与结肠癌转移的关系

目的探讨KAI1基因的表达与结肠癌转移的关系.方法用Northern blot及原位杂交分析法对10例正常人及24例结、直肠癌患者组织中的KAI1 mRNA表达分别进行检测.结果 North-ern blot分析显示,在正常及结肠癌组织中均有不同程度的KAI1 mRNA表达,原发性结肠癌伴淋巴结转移的KAI1 mRNA表达水平明显低于无淋巴结转移者(P＜0.01);原位杂交的KAI1 mRNA表达与Northern blot分析结果相一致,并发现KAI1 mRNA主要出现在原发性结肠癌细胞中,而肿瘤周围间质KAI1 mRNA相对呈低表达.结论 KAI1 mRNA表达在结肠癌的抗转移中具有一定的作用.     

糖类抗原15-3检测对乳腺癌骨转移的诊断价值

目的探讨糖类抗原15-3(CA15-3)在评价乳腺癌骨转移的价值。方法纳入在我院接受手术经病理学确诊的86例乳腺癌患者。采用99mTc-MDP核素骨显像检查并进一步病理学确认患者是否发生骨转移,记录发生骨转移的部位和数目。根据是否发生骨转移将患者分为2组。采用化学发光法检测所有患者血清CA15-3水平,比较2组患者CA15-3水平,分析CA15-3水平与骨转移累及部位数目的相关性;采用ROC曲线分析CA15-3水平对乳腺癌骨转移的诊断价值。结果乳腺癌骨转移组血清CA15-3水平高于无骨转移组(P<0.01)。骨转移累及部位数目与CA15-3水平的Spearman相关性分析结果显示两者并无相关性(r=-0.056,P=0.768)。ROC曲线分析结果显示CA15-3血清水平用于判断乳腺癌骨转移有显著意义(P=0.000)。当选CA15-3 24.675 U/mL为诊断界点,灵敏度为91.7%,特异度为93.8%。结论 CA15-3水平可以作为乳腺癌骨转移诊断的初步筛选指标。