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1.
目的通过测定细胞增生核抗原(PCNA),Ki67和溴脱氧尿嘧啶核苷(BudR),进一步判断食管和贲门正常和各级病变上皮细胞增生状况,并对三种指标进行比较。方法采用BudR渗入和免疫组化方法对辉县居民175例食管活检组织和45例贲门活检组织进行PCNA,Ki67和BudR测定。并定量分析活检组织每平方毫米内免疫阳性细胞数。结果在正常和不同级别病变上皮均出现不同程度的PCNA,Ki67和BudR阳性细胞。在食管组织,随病变从正常到增生和间变,PCNA和Ki67阳性细胞明显升高,比BudR在同级病变升高3倍;在贲门组织,Ki67阳性细胞数在正常上皮最低,随病变发展而升高。结论PCNA和Ki67免疫类型与食管和贲门病变发展关系密切。这些指标将有助于检测食管和贲门癌高危人群,以及Ⅱ级预防效果的评价。PCNA比较容易测量,可能是食管癌研究有效的指标。 相似文献
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慢性糜烂性胃炎的临床病理学及免疫组织化学研究 总被引:6,自引:3,他引:6
目的观察慢性糜烂性胃炎(CEG)的临床病理特征及粘膜上皮的p21,CEA表达和上皮细胞增生活性.结合粘膜G细胞数,探讨胃粘膜泌酸状态与病变的关系.方法CEG40例粘膜活检材料经光镜观察其组织病理学特征及Hp的感染.对其中31例采用免疫组化LSAB法标记p21,PCNA,CEA及胃泌素.结果CEG病变875%(35/40)见于胃窦粘膜,75%伴有不同程度的萎缩改变.粘膜上皮轻中度不典型增生率为200%(8/40),肠化率为275%,250%伴活动性炎;400%病例可见Hp感染.CEG粘膜上皮p21表达阳性率为677%,CEA表达为290%;明显高于对照的慢性浅表性胃炎组.PCNA表达以及G细胞数目,CEG组与对照组无显著差异(P>005).结论CEG具有一定恶性转化趋势,胃窦G细胞数、泌酸状态与CEG的发病无明显关联. 相似文献
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萎缩性胃炎中P53和PCNA的表达 总被引:2,自引:5,他引:2
目的了解P53,PCNA在胃粘膜癌前病变中的表达意义.方法慢性萎缩性胃炎(CAG)89例,慢性浅表性胃炎(CSG)20例和胃腺癌20例,作SP法观察P53和PCNA的表达.结果P53在CSG和CAG的单纯型、增生型和肠化型中不表达,异型性型中阳性表达率为190%(4/21).PCNA在CSG和CAG的单纯型、增生型、肠化型中无显著性差异(P>005);而CAG的异型性阳性细胞明显增多(P>001).结论P53表达与胃粘膜向癌的演变有关.PCNA随细胞增生活跃程度递增.二者结合对胃癌前病变的诊断、分级、治疗、预后有辅助价值. 相似文献
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食管癌p16基因缺失分析 总被引:3,自引:3,他引:0
目的观察抑癌基因p16在不同病程食管癌患者癌组织中的缺失情况.方法采用PCR方法检测26例食管癌患者癌组织标本及邻近正常食管上皮组织标本p16基因的缺失情况.结果在26例食管癌组织中检出14例标本有p16基因第2外显子缺失,缺失率538%,而癌旁正常食管组织均无缺失.在Ⅱ期至Ⅳ期食管癌(T2N0M0至T4N1M1)均检测到p16基因缺失.结论p16基因缺失可能在食管癌发生及发展中起较重要作用 相似文献
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表皮生长因子受体和PCNA在食管癌及癌前病变组织中的表达意义 总被引:6,自引:6,他引:0
目的探讨EGFR及PCNA的表达在食管癌发生发展过程中的临床意义.方法采用免疫组化SP法对各级食管鳞癌(Ⅰ级n=18,Ⅱ级n=30,Ⅲ级n=22)、各级不典型增生(轻度n=14,中度n=17,重度n=13)及正常食管粘膜(n=20)进行EGFR及PCNA检测.结果EGFR及PCNA阳性表达在各组食管病变中呈明显递增趋势,正常组、轻度及中度不典型增生组间EGFR及PCNA表达均有显著性差异,EGFR阳性表达分别为:0/20,3/14及10/17;PCNAⅠ,Ⅱ,Ⅲ,Ⅳ级表达分别为:19/20,1/20,0/20,0/20;7/14,6/14,1/14,0/14;2/17,7/17,7/17,1/17(P<005).鳞癌Ⅰ,Ⅱ级组间PCNA表达有显著性差异,PCNAⅠ,Ⅱ,Ⅲ,Ⅳ级表达分别为1/18,7/18,8/18,2/18;2/30,5/30,8/30,15/30(P<005);而中、重度不典型增生与Ⅰ级鳞癌组间均无显著性差异(P>005).结论食管中重度不典型增生者,其基因表达及增殖特性已具有明显的潜在恶性趋势,与食管癌的发生有直接关系,临床应对这类病例积极治疗并密切随访,以防癌变 相似文献
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胃癌组织P53蛋白和PCNA的表达意义 总被引:9,自引:5,他引:9
目的研究胃癌组织P53蛋白和增殖细胞核抗原(PCNA)的表达意义.方法应用ABC免疫组织化学技术检测76例保存资料完整并经病理学证实的胃癌石蜡包埋组织和30例正常胃粘膜P53蛋白及PCNA的表达进行同步检测.结果正常胃粘膜无P53蛋白表达,而644%(49/76)胃癌组织中P53蛋白表达阳性,P53蛋白表达阳性者其细胞增殖活性为821%±176%,明显高于P53蛋白表达阴性组的642%±143%(P<001).P53蛋白阳性率和PCNA计数值与胃癌临床分期及复发呈正相关(P<001).结论联合检测P53蛋白和PCNA对胃癌诊断、分期及预后有重要价值. 相似文献
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了解食管癌高发区Barrett's食管(BE)的患病率及BE与食管腺癌的关系。对331例河南省食管癌高发区农村自然人群进行了Barret's食管的内镜普查和病理组织学检查。同时对14例来自门诊的Barrett's食管病人的活检标本,用免疫组织化学染色的方法检测了抗癌基因P53的表达。结果显示:该人群Earrett's食管患病率为0.91%,返流性食管炎的检出率为7.6%。抗癌基因P53在14例Barrett's食管标本中阳性表达率为42%。其中50%阳性表达的标本伴有不典型增生。P53阳性表达可能是Barrett's食管上皮到食管腺癌发生过程中一个极早期的改变。对Barrett's食管伴有不典型增生或肿瘤基因表达阳性者应视为高危人群,对他们进行重点预防和阻断治疗是必要的。 相似文献
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青老年人胃癌P53及增殖细胞核抗原表达的对比 总被引:3,自引:5,他引:3
目的通过对老人青年胃癌中p53及增殖细胞核抗原(PCNA)表达的研究,探讨青老年人胃癌临床表现的分子生物学异同.方法应用过氧化物酶标记的链霉卵白素(SP)染色法对65例胃癌(青年组35例,≤35岁;老年组30例,≥60岁)进行p53蛋白及PCNA的免疫组织化学染色.并对胃癌细胞中PCNA阳性颗粒进行图象分析.结果p53和PCNA在青年人胃癌中的阳性率分别为657%和943%,均显著高于老年人(267%和500%,P<001).青年人胃癌中PCNA阳性颗粒平均光密度及积分光密度均显著高于老年人(P<005).结论p53及PCNA的高表达可能是青年人胃癌恶性度高,进展快,预后差的原因 相似文献
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目的:研究胃癌中P^53,PCNA和GSTπ表达及意义,方法:应用免疫组织化学S-P方法检测5例正常胃组织和54例胃癌组织中的P^53蛋白,增殖细胞核抗原(PCNA)和胎盘型谷胱甘肽S-转移酶(GSTπ)的表达水平。结果:5例正常组织中未见P^53PCNA和GSTπ表达。54例胃癌组织中P^53,PCNA和GSTπ阳性检出率分别为61.11%,70.37%和68.52%,表达阳性P^53和PCNA 相似文献
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了解食管癌高发区Barrett's食管(BE)的患病率及BE与食管腺癌的关系。对331例河南省食管癌高发区农村自然人群进行了Barret's食管的内镜普查和病理组织学检查。同时对14例来自门诊的Barrett's食管病人的活检标本,用免疫组织化学染色的方法检测了抗癌基因P_(53)的表达。结果显示:该人群Barrett's食管患病率为0.91%,返流性食管炎的检出率为7.6%。抗癌基因P_(53)在14例Barrett's食管标本中阳性表达率为42%。其中50%阳性表达的标本伴有不典型增生。P_(53)阳性表达可能是Barrett's食管上皮到食管腺癌发生过程中一个极早期的改变。对Barrett's食管伴有不典型增生或肿瘤基因表达阳性者应视为高危人群,对他们进行重点预防和阻断治疗是必要的。 相似文献
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食管贲门癌冷冻对免疫功能的调节 总被引:2,自引:0,他引:2
目的研究食管贲门癌患者食管腔内冷冻对免疫功能的调节作用.方法食管癌15例,贲门癌5例;男11例,女9例.先行食管腔内冷冻,再行开胸手术切除。用光镜及电镜系统观察癌细胞变化及局部淋巴反应,以及血中免疫球蛋白、补体C3及淋巴细胞转化试验LTT.结果食管贲门癌原发灶周围淋巴结呈广泛性肿大,癌细胞坏死,可见异物巨细胞吞噬癌细胞,淋巴结呈滤泡增生反应.冷冻前后免疫球蛋白IgG、IgM无明显差异;LTT由冷冻前的380%增加到448%,IgA由冷冻前的216g/L增加到232g/L,C3也由冷冻前13g/L增加到20g/L.结论食管贲门癌腔内冷冻能调节机体的免疫功能和杀伤癌细胞. 相似文献
12.
Li-Dong Wang Qi Zhou Yu-Chun Zhang Xiao-Fang Li Wen-Ping Wang Ling He Shan-Shan Gao Young-Xin Li 《World journal of gastroenterology : WJG》1997,3(2):80-80
AIM: To study changes in p53 expression and cell proliferation in esophageal epithelia of subjects from high or low esophageal cancer incidence areas in Henan Province to understand their molecular basis.METHODS: Esophageal endoscopic mucosa biopsies were acquired and histopathological examinations were performed on 220 subjects from high esophageal cancer incidence areas and 50 subjects from low incidence areas in Henan Province. Esophageal epithelia were diagnosed as normal, basal cell hyperplasia or dysplasia based on cell morphology and tissue structure. Immunohistochemistry avidin biotin peroxidase complex (ABC method) was performed to analyze alterations in p53 and proliferating cell nuclear antigen (PCNA) expression in normal epithelia and epithelia with different lesion severities. The numbers of p53-positive and PCNA-positive cells were counted.RESULTS: p53- and PCNA-positive nuclei were present in esophageal epithelia from subjects from both high and low incidence areas. The number of PCNA-positive cells gradually increased with lesion severity for both the high and low incidence areas. The number of p53-positive cells was higher in high incidence areas compared to low incidence areas, and rapidly increased with lesion severity. p53 expression positively correlated with PCNA expression.CONCLUSION: The number of both p53- and PCNA-positive cells increased with lesion severity. p53 expression was higher in subjects from high esophageal cancer incidence areas compared to those from low incidence areas. These results may shed light into the molecular basis for the geographical distribution of esophageal cancer. 相似文献
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The objective of the study was to assess the differences in clinical and pathological characteristics between esophageal stromal tumor and leiomyoma. Data from 93 esophageal stromal tumors and leiomyomata cases were retrospectively analyzed, including clinical symptoms, endoscopic features, pathological characteristics, immunohistochemistry (IHC), and treatment. All cases underwent endoscopic ultrasonography examination before treatment. Lesions arising from the muscularis mucosa were resected by endoscopic mucosal resection or endoscopic submucosal dissection. Lesions arising from the muscularis propria were resected by surgery. All specimens were examined by IHC. Patients were followed up after endoscopic mucosal resection or endoscopic submucosal dissection. No difference was observed in clinical symptoms and endoscopic features between the two groups. Endoscopic ultrasonography demonstrated all lesions to be hypoechoic and well circumscribed. Most lesions >2 cm had heterogeneous internal ultrasound signal. In esophageal stromal tumor, 100% (29/29) were CD117‐positive and DOG‐1‐positive; 72.4% (21/29) and 51.7% (15/29) were CD34‐positive and smooth muscle actin‐positive, respectively. In esophageal leiomyomata, 100% (64/64) were smooth muscle actin‐positive and desmin‐positive; 100% were CD117‐negative and DOG‐1‐negative. No local recurrence was detected in followed up patients (n = 49) after an average of 1.8 years (1.0–3.0 years). IHC analyses are important for distinguishing esophageal stromal tumor from leiomyoma. Early endoscopic resection is an effective treatment option for esophageal stromal tumors >1 cm. 相似文献
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GAO Shan-Shan ZHOU Qi LI Yong-Xin BAI Yong-Min ZHENG Zuo-Yu ZOU Jian-Xiang LIU Gang FAN Zong-Min QI Yi-Jun ZHAO Xin WANG Li-Dong 《World journal of gastroenterology : WJG》1998,4(4)
AIM To investigate the pathogenesis of gastric cardia and the distal part of stomach cancer and to further characterize the histopathogenesis model for gastric cardia cancer from the high-risk population for esophageal cancer.METHODS Mass survey with endoscopic mucosa biopsy and histopathological examination were carried out on 226 subjects aged above 30 years. Three biopsies were collected one each from the middle part of the esophagus, the gastric cardia and the pyloric antrum. The biopsy tissue was fixed with 85% alcohol and paraffin-embedded.RESULTS The incidence of intestinal metaplasia and dysplasia at gastric cardia epithelium was higher than that at the pyloric antrum from the subjects in the same area. And there were high incidences of both esophageal and gastric cardia cancer, but a low incidence of gastric cancer at the distal part of the stomach.CONCLUSION There might be different etiology and pathogenesis of gastric cardia and pyloric cancer at the distal part of the stomach. 相似文献
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Ryu Ishihara Yoji Takeuchi Rika Chatani Takashi Kidu Takuya Inoue Noboru Hanaoka Sachiko Yamamoto Koji Higashino Noriya Uedo Hiroyasu Iishi Masaharu Tatsuta Yasuhiko Tomita Shingo Ishiguro 《Diseases of the esophagus》2010,23(6):480-486
Narrow‐band imaging (NBI) is a novel, noninvasive optical technique that uses reflected light to visualize the organ surface. However, few prospective studies that examine the efficacy of NBI screening for esophageal cancer have been reported. To compare the diagnostic yield of NBI endoscopy for screening of squamous mucosal high‐grade neoplasia of the esophagus between experienced and less experienced endoscopists. Patients with a history of esophageal neoplasia or head and neck cancer received NBI endoscopic screening for esophageal neoplasia followed by chromoendoscopy using iodine staining. Biopsy specimens were taken from iodine‐unstained lesions and the histological results of mucosal high‐grade neoplasias served as the reference standard. The primary outcome was the sensitivity of NBI for detecting new lesions. The secondary outcome was the positive predictive value of NBI and the sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of NBI in a per lesion basis. A total of 350 patients (170 by experienced endoscopists and 180 by less experienced endoscopists) underwent endoscopic examination. A total of 42 new mucosal high‐grade neoplastic lesions (25 in the experienced endoscopist group and 17 in the less experienced endoscopist group) were detected. In the per‐lesion‐based analysis, the sensitivity was significantly higher in the experienced endoscopist group (100%; 25/25) compared with the less experienced endoscopist group (53%; 9/17) (P < 0.001). The positive predictive value of NBI was higher in the experienced endoscopist group than in the less experienced endoscopist group (45%, 25/55 vs. 35%, 9/26), although the difference was not significant (P = 0.50). The sensitivity of NBI in the less experienced endoscopist group was 43% in the former half of patients, and increased to 60% in the latter half of patients. In the per‐patient‐based analysis, the sensitivity of NBI was significantly higher in the experienced endoscopist group (100%) than in the less experienced endoscopist group (100 vs. 69%, respectively; P = 0.04). The positive predictive values of the experienced endoscopist group and the less experienced endoscopist group were similar, and were 48 and 47%, respectively. In conclusion, compared with the gold standard of chromoendoscopy with iodine staining, the sensitivity of NBI for screening of mucosal high‐grade neoplasia was 100% with the experienced endoscopists but was low with the less experienced endoscopists. Electronic chromoendoscopy with NBI is a promising screening tool in these high‐risk patients with esophageal mucosal high‐grade neoplasia, particularly when performed by endoscopists with experience of using NBI. 相似文献
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高龄低肺功能食管癌患者手术治疗的研究 总被引:16,自引:1,他引:16
目的 :探讨高龄低肺功能食管癌病人外科手术治疗适应症、手术方式的选择及术后呼吸机的应用。方法 :4 6例 70岁以上食管癌患者依据最大通气量占预计值百分比 (MVV ,% )分为 2组 :第一组 ,MVV≤ 5 0 % 2 2例 ,第二组为对照组 :MVV >5 0 % 2 4例 ;第一组再分 2组 :1组MVV <4 0 % 3例 ,其中 2例术前FEV1 FVC <4 5 % ,1例FEV1 FVC >4 5 % ;2组 :MVV 4 0 %~ 5 0 % 19例 ,FEV1 FVC均 >4 5 %。第一组患者术后均使用呼吸机辅助呼吸。对比研究 1组、2组患者及对照组呼吸衰竭、心律失常发生情况。结果 :各组无手术死亡 ;1组中 2例 (FEV1 FVC <4 5 % )同时发生呼吸衰竭及心房颤动 ;2组中 4例病人出现心律失常 ,无呼吸衰竭 ;对照组中 5例病人出现心律失常 ,无呼吸衰竭。结论 :由于手术技术提高和术后呼吸机的应用 ,可相对扩大手术适应征 相似文献
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Chen HB Chen L Zhang JK Shen ZY Su ZJ Cheng SB Chew EC 《World journal of gastroenterology : WJG》2001,7(6):788-791
AIM:To explore the etiologic role of HPV infection inesophageal carcinoma,and the association of HPV-16 E6with the nuclear matrix of carcinoma cells.METHODS:Two esophageal carcinoma cell lines,EC/CUHK1 and EC/CUHK2,were tested for HPV-16 E6subgenetic fragment by polymerase chain reactionarnplification of virus DNA associated nuclear matrix.RT-PCR and immunocytochemistry were also used to visualizethe expression of E6 subgene in the cells.RESULTS:The HPV-16 E6 subgenetic fragment was found tobe present in nuclear matrix-associated DNA,E6oncowprotein localized in the nucleus where it is tightlyassociated with nuclear matrix after sequential extraction inEC/CUHK2 cells.It was not detected,however,in EC/CUHK1 cells.CONCLUSION:The interaction between HPV-16 E6 andnuclear matrix may contribute to the virus inducedcarcinogenesis in esophageal carcinoma. 相似文献