Overexpression of Gli1 in cancer interstitial tissues predicts early relapse after radical operation of breast cancer

Objective:To investigate whether Gli1 expression is important in relapse after radical operation of breast cancer.Methods:Using immunohistochemistry,Gli1 expression was analyzed in human primary breast cancer(n=284) and paracancerous tissues(n=20),and also in local lymph nodes(n=28) and metastatic lymph nodes(n=28).Results:Initial analysis of Gli1 expression in a small cohort of 20 breast tumors and their paracancerous tissues showed a tendency towards Gli1 overexpression in breast cancer tissues(P<0.001).Further,Gli1 expression in 284 breast cancer tissue samples was analyzed and a significant correlation was found between increased expression of nuclear Gli1 and unfavorable recurrence-free survival(RFS)(P<0.05).The nuclear expression of Gli1 in metastatic lymph nodes following relapse after radical operation was much higher than that in the local lymph nodes of primary carcinoma(P<0.05).Most interestingly,the expression of Gli1 was much higher in the interstitial tissues of the relapsed group than of the non-relapsed group(P<0.001).Conclusions:Breast cancer shows a high prevalence of Gli1 expression,which is significantly correlated with aggressive features and unfavorable RFS.Nuclear Gli1 overexpression,especially in the interstitial tissues,signified early relapse after radical operation of breast cancer.     

RELATIONSHIP BETWEEN EXPRESSIONS OF P38 PROTEIN IN HUMAN BREAST CARCINOMA AND LYMPH NODES METASTASIS

Objective： To detect the change of p38 protein expression and investigate the relationship of p38 and lymph nodes metastasis in human breast carcinomas. Methods： Sixty breast cancer cases were checked by S-P immunohistochemistry technique and 30 breast cancer cases were examined by Western Blot. Results： Immunohistochemical results showed that p38 protein was observed in breast cancer and normal cytoplasm. P-p38 was positive in nucleus in breast cancer. P38 protein expressed positively in 29 out of 38 patients who had lymph nodes metastasis （positive rate 76.3%） and in 9 out of 22 patients who had no lymph nodes metastasis （positive rate 40.9%）. There was a significant difference between these two groups （P〈0.01）. The positive rate of p-p38 in patients who had lymph nodes metastasis was 68.4%, and the positive rate in patients who had no metastasis was 36.4%, and there was a significant difference between these two groups （P〈0.05）. The result of western blot showed that the protein contents of p38 and p-p38 in patients with metastasis was higher than those in patients without metastasis （P〈0.05）. P38 and p-p38 protein expressions had relation with clinical pathological grades in breast cancer, higher in grade Ⅲ than in grade Ⅰ, Ⅱ （P〈0.05）, while had no relation with patients＇ age and tumor size （P〉0.05）. Conclusion： p38 and p-p38 protein expressions had relationship with lymph nodes metastasis and the levels of p38 and p-p38 protein expression in groups with lymph nodes metastasis were higher than in groups without lymph nodes metastasis. P38 and p-p38 protein expressions had relationship with clinical grades and had no relationship with patients＇ age and tumor size.     

Emmunohistochemical study on angiogenesis of axillary lymph node metastasis in human invasive breast carcinoma

The axially lymph node is one of the earliest and most common metastatic positions of breast carcinoma. Hence, axillary lymph node status (metastasis or no) and the number of metastatic lymph nodes are considered to be important indicators which affect prognosis of breast cancer patients. It has been widely confirmed that malignant solid tumor growth must be dependent upon angiogenesis/neovascularization. Considerable evidence has shown that microvessel density (MVD) of breast cancer tissue c…     

The Significance of CXCR4 Expression for the Prediction of Lymph Node Metastasis in Breast Cancer Patients

OBJECTIVE The chemokine receptor(CXCR4)CXC chemokine receptor 4)plays an important role in cancer metastasis.We therefore studied differential expression of the CXCR4,as well as that of the biomarker HER2,so as to evaluate whether these biomarkers can be used to predict axillary lymph node metastasis in breast cancer patients. METHODS Immunohistochemistry was used to evaluate the CXCR4 and HER2 expressions and to examine the paraffin sections of the breast cancers at various stages.Positive lymph node expression was found in 80 of the cases,and in 7 there was negative expression. RESULTS Compared to the cases with negative lymph nodes, there was a high expression of CXCR4(26.3% vs.14.3%,P=0.013), and an over-expression of HER2(28.8% vs.14.3%,P=0.011). Moreover,there was a direct correlation between the CXCR4 and HER2 expressions and the tumor staging(P=0.000)and lymph node metastasis(P=0.032).When the two biomarkers,i.e.CXCR4 and HER2,were concurrently labeled,a high expression of one of the biomarkers could be seen in the cases with positive lymph nodes(51.3% vs.28.6%,P<0.003). CONCLUSION The chemokine receptor,CXCR4,is a new-type biomarker in predicting axillary lymph-node metastasis in breast cancers.Compared with the other markers,such as HER2 etc., assessment of CXCR4 can improve the prediction of the presence and extent of lymph node involvement.     

E-钙黏蛋白在鼻咽癌组织中的表达水平及其与颈部淋巴结转移的关系

Objective To investigate the expression of E-cadherin in nasopharyngeal carcinoma ( NPC) and its relationship with cervical lymph node metastasis. Methods The expression of E-cadherin in 80 patients with NPC was detected by immunohistochemistry. Results Lower expression of E-cadherin was associated with advanced N-stage of the tumor ( P = 0. 018 ). There was no significant correlation between the expression of E-cadherin and lymph node size ( P = 0.435 ). The expression of E-cadherin was higher in patients with cervical lymph node metastasis limited to a single area than that distributing in some scattered areas (P = 0. 000). There was a trend that the expression of E-cadherin in the cases with the tumor and lymph nodes in the same side was higher (56. 5% ) than that in the patients with bilateral lymph node metastases (32. 6% ) , however, the difference was not significant (P =0. 059). The expression rates of E-cadherin in patients with lymph node metastasis in levels Ⅱ , Ⅲ and Ⅴa were higher than that in levels Ⅰ , Ⅳ, Vb and Ⅵ, but with a non-significant difference (P = 0.059). Conclusion The expression of E-cadherin has influence on the lymph node metastasis in nasopharyngeal carcinoma. E-cadherin expression is negatively correlated with the numbers of the lymph node metastases and the metastasis distance, i. e. a lower expression of E-cadherin leads to an advanced N-stage. The lymph node metastasis of nasopharyngeal cancer from above to below is more considerably influenced by E-cadherin expression than the metastasis towards contralateral lymph nodes.     

E-钙黏蛋白在鼻咽癌组织中的表达水平及其与颈部淋巴结转移的关系

Objective To investigate the expression of E-cadherin in nasopharyngeal carcinoma ( NPC) and its relationship with cervical lymph node metastasis. Methods The expression of E-cadherin in 80 patients with NPC was detected by immunohistochemistry. Results Lower expression of E-cadherin was associated with advanced N-stage of the tumor ( P = 0. 018 ). There was no significant correlation between the expression of E-cadherin and lymph node size ( P = 0.435 ). The expression of E-cadherin was higher in patients with cervical lymph node metastasis limited to a single area than that distributing in some scattered areas (P = 0. 000). There was a trend that the expression of E-cadherin in the cases with the tumor and lymph nodes in the same side was higher (56. 5% ) than that in the patients with bilateral lymph node metastases (32. 6% ) , however, the difference was not significant (P =0. 059). The expression rates of E-cadherin in patients with lymph node metastasis in levels Ⅱ , Ⅲ and Ⅴa were higher than that in levels Ⅰ , Ⅳ, Vb and Ⅵ, but with a non-significant difference (P = 0.059). Conclusion The expression of E-cadherin has influence on the lymph node metastasis in nasopharyngeal carcinoma. E-cadherin expression is negatively correlated with the numbers of the lymph node metastases and the metastasis distance, i. e. a lower expression of E-cadherin leads to an advanced N-stage. The lymph node metastasis of nasopharyngeal cancer from above to below is more considerably influenced by E-cadherin expression than the metastasis towards contralateral lymph nodes.     

胃癌组织中Ets-1的表达及其与血管生成临床病理特征和预后的关系

Objective To investigate the expression of Ets-I in gastric carcinoma,pars-cancerous tissue and metastatic lymph nodes,and to determine the relationship between Ets-1 expression and clinicopathological features,angiogenesis and survival of patients with gastric carcinoma.Methods Gastric carcinoma tissue microarray was used to determine Ets-I protein expression by SP immunohistochemical staining in 189 advanced gastric cancer,54 papacancerous tissues,41 metastatic lymph nodes and 32 control tissues.Results The positive rates for Ets-1 expression of the carcinoma,paracancerous and control tissues were 71.4 %,29.6% and 18.8%,respectively,with a significant difference among the three groups(P ＜0.01).In the cancer tissues,the positive rate of Ets-1 protein expression was significantly associated with depth of invasion and lymph node metastasis(P ＜0.01),but not associated with degree of differentiation,Lauren's histological type,sex,age,and size of tumor(P ＞0.05).The positive rates for Ets-1 expression of the 41 gastric cancer and 41 metastatic lymph nodes were significantly different(P ＜0.05).In metastatic lymph nodes,the positive rate for Ets-1 expression was higher.The MVD in Ets-1 positive tumors was higher than that in the Ets-1 negative tumors,with a significant difference(P ＜ 0.05).Kaplan-Meier survival analysis showed that the survival time of Ets-1-negative patients was longer than that of Ets-1-positive patients (P ＜0.05).Cox regression analysis showed that Ets-1 expression was not an independent prognostic factor of gastric carcinoma.Conclusion A higher expression of Ets-1 is involved in carcinogenesis,development,invasion,and metastasis of gastric cancer.Ets-1 plays an important role in angiogenesis in gastric cancer.Ets-1 is a useful marker for predicting the outcome for patients with gastric carcinoma,though it is not an independent prognostic indicator.     

EXPRESSION OF E-CADHERIN/CATENIN COMPLEX IN BREAST CANCER

Objective： To detect the expressions of E-cadherin, α-catenin and β-catenin and analyze the relationship between Ecadherin-catenin adhesion complex and clinicopathological features in breast cancer. Methods： The expressions of E-cadherin, α-cadherin and β-catenin in specimens of 54 breast cancer, 21 normal breast tissues around tumor, 15 breast hyperplasia of usual type and 15 breast atypical hyperplasia were detected by immunohistochemical method. Results： In 21 normal breast tissues, E-cadherin and α-catenin were expressed on cell membrane of ductal and acinic cells, showing cellular contour and border among cells. The staining character of the three proteins in breast hyperplasia of usual type was the same as that in normal breast tissue. In breast atypical hyperplasia, the abnormal expression rates of E-cadherin, α-catenin and β-catenin were 6.7%, 13.3% and 26.7%, respectively. The total abnormal expression rate of E-cadherin-catenin complex was 33.3%. In breast cancer, the abnormal expression rates of E-cadherin, α＋catenin and β-catenin were 51.9%, 63.0% and 61.1%, respectively. The total abnormal expression rate of E-cadherin-catenin complex was 88.9%. Abnormal expression of E-cadherin and α-catenin were significantly correlated with histological grade. Abnormal expressions of α-catenin and β-catenin were significantly correlated with TNM staging, axillary lymph nodes metastasis and postoperative distant metastasis. Abnormal expression of E-cadherin-catenin complex was correlated with TNM staging, histological grade and axillary lymph nodes. Abnormal expression of β-catenin was negatively correlated with expression of HER-2. COX multiple factor analysis showed that E-cadherin or α-catenin or β-catenin was not independent prognostic indicator. Conclusion： Abnormal expressions of E-cadherin, α-catenin and β-catenin frequently occur in breast cancer. Abnormal expression of E-cadherin-catenin complex is correlated with differentiation disturbance and metastasis. Combined measurement of E-caherin, α-catenin and β-catenin may improve accuracy and sensitivity of predicting metastasis and prognosis of breast cancer.     

Clinical significance of XRCC1 gene expression in human breast cancer and its cell and molecular mechanisms北大核心CSCD

Objective: To investigate the expression of X-ray repair cross complementing 1 (XRCC1) in human breast cancer and its relationship with the clinical characteristics, and to analyze the effects of XRCC1 over-expression on the proliferation and migration of breast cancer MB-231 cells and the molecular mechanism. Methods: The expression level of XRCC1 mRNA in breast cancer cell lines and human breast cancer tissues was detected by real-time fluorescent quantitative PCR. The expression of XRCC1 protein in human breast cancer tissues was detected by immunohistochemistry. The relationship between the expression of XRCC1 protein and the clinicopathological characteristics of breast cancer patients was analyzed. The pcDNA3.1(+)-Flag-XRCC1 plasmids were transfected into breast cancer MB-231 cells for the overexpression of XRCC 1 gene. Then the proliferation activity was detected by CCK-8 and soft agar plate clone formation assay. The cell cycle and apoptosis were detected by FCM method. The cell migration and invasion were detected by Transwell chamber assay. The expressions of cell cycle-, apoptosis- and migration-related proteins were detected by Western blotting. Results: The expression level of XRCC1 mRNA was significantly decreased in most breast cancer cell lines (all P < 0.001). As compared with the normal mammary epithelium and the paired adjacent breast tissues, the expression levels of XRCC1 mRNA and protein were downregulated in human breast cancer tissues (all P < 0.001). The expression level of XRCC1 mRNA was positively correlated with the prognosis of breast cancer patients (γ 2 =0.052, P =0.046), and XRCC1 protein expression was correlated with tumor diameter, lymph node metastasis, histological grade and TNM stage (all P < 0.05). After the overexpression of XRCC 1 gene, the proliferation, colony formation, invasion and migration of breast cancer MB-231 cells were significantly inhibited (all P < 0.01), the cell cycle was significantly blocked in G1 phase (P < 0.001), and the apoptosis rate was significantly increased (P < 0.001). Furthermore, the expressions of p21, p27, Bax, cleaved caspase-3 and E-cadherin were significantly upregulated (all P < 0.001), while the expressions of cyclin-dependent kinase 4/6 (CDK4/6), cyclin D1, Bcl-2, N-cadherin and vimentin were down-regulated (all P < 0.001) in MB-231 cells with XRCC1 overexpression. Conclusion: XRCC1 expression is down-regulated in breast cancer cell lines and tissues, and its expression level is positively correlated with the prognosis of breast cancer patients. Restoring XRCC 1 gene expression can inhibit cell growth, migration and invasion, and can induce apoptosis. So XRCC1 may be a potential tumor suppressor regulating the occurrence and development of human breast cancer. © 2019 by TUMOR.     

Relationship between Lymphatic Vessel Density and Lymph Node Metastasis of Invasive Micropapillary Carcinoma of the Breast

OBJECTIVE To investigate the relationship between lymphatic vessel density and lymph node metastasis of invasive micropapillary carcinoma (IMPC) of the breast. METHODS The immunohistochemical study for vascular endothelial growth factor-c (VEGF-C), VEGF Receptor-3 (VEGFR-3) and lymphatic vessel density of 51 cases of IMPC were performed, and lymph node metastases were examined by microscopic analysis of these cases. RESULTS In IMPC, VEGF-C was expressed in the cytoplasm and/or on the membrane of the tumor cells, and the expression of VEGF-C showed a positive correlation with lymph node metastasis (P<0.01). Lymphatic vessel density was determined by the number of micro-lymphatic vessels with VEGFR-3 positive staining. Lymphatic vessel density was positively correlated with VEGF-C expression (P<0.01) and lymph node metastasis (P<0.01). The percentage of IMPC in the tumor was not associated with the incidence of lymph node metastasis. The metastatic foci in lymph nodes were either pure or predominant micropapillary carcinoma. CONCLUSION The results suggested that VEGF-C overexpression stimulated tumor lymphangiogenesis, and the increased lymphatic vessel density may be the key factor that influenced lymph node metastasis of IMPC.     

CD44v6在乳腺浸润性导管癌组织中的表达及其临床意义

背景与目的:近年研究表明,CD44v6与乳腺癌的发生、侵袭和转移密切相关,但其与乳腺癌预后关系的报道结果并不一致。本研究探讨CD44v6在乳腺癌和乳腺癌旁非癌组织中的表达及其与乳腺癌临床病理因素的关系以及对乳腺癌患者预后的预测意义。方法:采用免疫组织化学SP法检测84例乳腺浸润性导管癌和20例癌旁非癌乳腺组织中CD44v6的表达。采用SPSS10.0软件统计分析CD44v6表达与各病理因素的关系,采用Cox比例风险模型分析影响预后的因素。结果:CD44v6在乳腺浸润性导管癌上皮细胞的表达(78.6%)明显高于癌旁非癌乳腺组织上皮(5.0%),差异有显著性(P<0.05);CD44v6的表达与乳腺癌的TNM分期、肿瘤大小、淋巴结转移状况密切相关;中位随访时间为60个月,CD44v6阴性组的总体生存情况优于CD44v6阳性组,有显著性差异(P<0.05),而且随着CD44v6表达的增强,总体生存曲线有逐渐下降的趋势。Cox比例风险模型多因素的预后分析结果显示,ER、TNM分期、CD44v6均是影响预后的独立因素(P<0.05)。结论:CD44v6在乳腺癌组织中的表达明显升高;CD44v6的表达与乳腺癌患者的TNM分期、肿瘤大小、淋巴结转移呈正相关;CD44v6表达升高可作为预测乳腺癌预后的独立指标之一。     

Study of indoleamine 2,3-dioxygenase expression in patients with breast cancer

We evaluated the clinical significance of indoleamine 2,3-dioxygenase (IDO) in breast cancer. Operative specimens obtained from 30 patients with breast cancer were investigated by semiquantitative RT-PCR with specific primers against IDO. The correlations among IDO expression, clinicopathologic factors and prognosis were studied. The expression of IDO was observed in 100%, both of the cancer specimens and the non-cancer specimens. The IDO expression of the cancer specimens was higher than the non-cancer specimens. The expression of IDO did not correlate to histologic classification, tumor size, lymphatic invasion, venous invasion and lymph nodes metastasis, but correlated to clinical stage and the serum level of immunosuppressive acidic protein (IAP). There were no correlations for a survival rate after surgery between the high IDO level group and the one. The serum IDO levels of cancer patients were higher than that of a healthy volunteer measured by semiquantitative RT-PCR and HPLC. It is suggested that the expression of IDO in breast cancer patients may play a critical role for immunosuppression in those patients.     

ER、PR、Her-2在乳腺癌中的表达及临床意义

目的探讨雌激素受体(ER)、孕激素受体(PR)及癌基因Her-2在乳腺癌中的表达及其临床意义。方法 应用免疫组化法检测76例乳腺癌ER、PR、Her-2的表达。结果 ER的表达与TNM分期和淋巴结转移呈负相关;PR的表达与淋巴结转移和TNM分期无相关性;Her-2的表达与淋巴结转移和TNM分期呈正相关。结论 乳腺癌患者ER、Her-2检测可以帮助判断乳腺癌预后。     

乳腺癌髓系来源抑制细胞中IDO表达与调节性T细胞相关性及其临床意义的研究

  目的  研究不同期别乳腺癌组织中CD33+髓系来源抑制细胞(MDSCs)和Foxp3+调节性T细胞(Tregs)的分布情况, 探讨MDSCs中吲哚胺2, 3-双加氧酶(IDO)表达与Tregs分布关系及其临床意义。   方法  收集天津医科大学附属肿瘤医院2005年1月至2007年1月手术患者的乳腺癌石蜡切片50例, 采用免疫组织化学单染方法对肿瘤局部CD33+MDSCs和Foxp3+Tregs分布和比例进行检测; 采用免疫组织化学双染方法检测肿瘤原位浸润MDSCs中IDO的表达情况; 分析MDSCs中IDO表达与Tregs分布、比例及其他临床病理资料之间的相关性。   结果  Foxp3+Tregs和CD33+MDSCs细胞在乳腺癌组织中呈散在性分布。MDSCs中IDO表达水平与腋窝淋巴结转移密切相关(P < 0.05)。Foxp3+Tregs高表达组中MDSCs中IDO表达水平显著高于Foxp3+Tregs低表达或不表达组(P < 0.05)。   结论  MDSCs中IDO过表达可能有利于Tregs的募集和乳腺癌的转移。      

畸胎癌生长因子-1在基底细胞样乳腺癌中的表达及其临床意义

目的 检测畸胎癌生长因子-1（CR-1）蛋白和CD34蛋白在基底细胞样乳腺癌（BLBC）中的表达情况,探讨CR-1在BLBC血管生成和侵袭转移中的作用.方法 采用免疫组织化学法检测60例BLBC、40例非BLBC和20例正常乳腺组织中CR-1蛋白和CD34蛋白的表达,并分析其与BLBC患者CD34标记的微血管密度（MVD）值、年龄、病理组织学分期、淋巴结转移等的关系.结果 CR-1蛋白在BLBC中表达率为70.00％（42/60）,明显高于非BLBC的47.50％（19/40）和正常乳腺组织的20.00％（4/20）（均P＜0.05）,其表达与BLBC患者的临床分期、淋巴结转移、MVD值及肿瘤大小呈正相关（均P＜0.05）;而与患者年龄无明显相关性（P＞0.05）.结论 CR-1的表达上调可能与BLBC的血管生成及发生、发展过程有关.CR-1蛋白促进血管生成的作用可能是其促进肿瘤的局部侵袭和远处转移的因素.     

Study of indoleamine 2,3-dioxygenase expression in patients of thyroid cancer

We evaluated a clinical significance of indoleamine 2,3-dioxygenase (IDO) in thyroid cancers. Operative specimens obtained from 20 patients with thyroid cancers were investigated by semi-quantitative RT-PCR with specific primers against IDO. The correlations among IDO expression, clinicopathologic factors and prognosis were studied. An expression of IDO was observed in 100% for both of the cancer specimens and the non-cancer specimens. The IDO expression of cancer specimens was significantly higher than the non-cancer specimens. The expression of IDO did not correlate to histologic classification, tumor size, lymphatic invasion, venous invasion and lymph nodes metastasis, but it was correlated to clinical stage. There was no correlation for survival rate after surgery between the high IDO level group and low IDO level group. The serum IDO levels of cancer patients were higher than that of a healthy volunteer measured by semi-quantitative RT- PCR and HPLC. It is suggested that the expression of IDO in thyroid cancer patients may play critical role for immunosuppression of those patients.     

胃癌组织中CD105和CD31的表达及其生物学行为的关系

 目的 探讨CD105，CD31在胃癌组织中的表达情况及其与胃癌生物学行为之间的关系。方法 采用免疫组化S-P单染法检测63例胃癌组织及20例慢性胃炎组织中CD105，CD31所标染的微血管密度值（MVD）。结果 （1）胃癌组织中CD105-MVD平均值为40.97±15.67，高于CD31-MVD平均值25.87±10.54（P<0.01），而二者均明显高于慢性胃炎组织中测得的相应值，分别为（19.55±7.55，20.65±7.44）（P <0.01）。（2）CD105的表达与胃癌的浸润深度、分期、淋巴结转移呈正相关（P <0.01），而与肿瘤大小、分级无关（P >0.05）。结论 （1）CD105在胃癌组织微血管中高表达，在标染胃癌组织MVD方面，CD105比CD31具有明显优越性。（2）CD105的表达与胃癌的生物学行为密切相关；其检测对更准确地确定肿瘤的临床分期、指导治疗、判断预后具有积极的临床意义。     

MMP-2、VEGF、CD105和Ki-67在小细胞肺癌中的表达及预后相关性研究

目的 探讨基质金属蛋白酶-2（MMP-2）、血管内皮生长因子（VEGF）、CD105和Ki 67在小细胞肺癌（SCLC）中的表达及其与临床病理特征和预后的关系。方法 收集42例SCLC和8例正常肺组织标本,采用免疫组化SP法检测MMP-2、VEGF、CD105和Ki 67在SCLC和正常肺组织中的表达,并分析其与SCLC临床病理特征和预后的关系。结果 42例SCLC组织中MMP-2、VEGF、CD105和Ki-67的阳性表达率分别为50.0％、66.7％、81.0％和64.3％,在8例正常肺组织中分别为12.5％、12.5％、0和0,差异均有统计学意义（P＜0.05）。MMP-2、VEGF、CD105的表达均与肿瘤直径、淋巴结转移、远处转移和临床分期有关,Ki-67表达与淋巴结转移有关（P＜0.05）。MMP-2、VEGF表达均与CD105和Ki-67有相关性（P＜0.05）,且MMP-2和VEGF表达呈正相关（r=0.447,P＜0.05）。单因素分析显示,肿瘤直径、淋巴结转移、远处转移、临床分期、MMP-2、VEGF、CD105和Ki 67均与总生存有关。结论 MMP-2、VEGF、CD105和Ki-67均为SCLC预后不良指标,MMP-2和VEGF可以协同促进肿瘤的新生血管形成和细胞增殖。     

人乳腺癌组织中D2-40标记的微淋巴管密度与血管内皮生长因子C表达的关系

目的 探讨人乳腺癌组织中D2-40标记的微淋巴管密度(LMVD)与血管内皮生长因子C(VEGF-C)表达的关系.方法 收集102例乳腺癌、25例乳腺纤维腺瘤和10例正常乳腺组织标本,采用免疫组化SP法检测D2-40单抗标记的LMVD和VEGF-C蛋白的表达水平,采用原位杂交法检测VEGF-C mRNA的转录水平. 结果 102例人乳腺癌组织中,D2-40标记微淋巴管的阳性率为76.5%,高于乳腺纤维腺瘤组织.癌周组织的LMVD为30.1个微淋巴管/100倍视野,显著高于癌中心区、正常乳腺组织和乳腺纤维腺瘤组织的LMVD (均P＜0.01),且与乳腺癌腋窝淋巴结转移数目明显相关(r=0.964,P<0.01).102例乳腺癌组织中,VEGF-C蛋白和mRNA的阳性表达率分别为55.9%和59.8%,均高于乳腺纤维腺瘤组织(χ2=11.653,P=0.003;χ2=10.345,P=0.006),且与淋巴结转移数目、临床分期、c-erbB-2和p53基因的表达相关(均P<0.05).VEGF-C蛋白和mRNA的表达水平均与D2-40标记的LMVD相关(P<0.05),尤其见于癌周组织中(P<0.01).结论 D2-40单抗标记的LMVD与VEGF-C的表达有密切关系,VEGF-C参与的乳腺癌微淋巴管的生成在乳腺癌淋巴转移中有重要意义.     

乳腺癌组织中CD105 mRNA的表达及其临床病理意义

目的　探讨CD10 5mRNA在乳腺癌中的表达以及与乳腺癌临床病理特征的关系。方法以β actin基因为参照 ,应用逆转录聚合酶链反应 (RT PCR)技术 ,检测 4 0例乳腺癌患者的肿瘤中心处癌组织、肿瘤边缘癌组织及癌旁正常腺体组织中CD10 5的表达。应用条带密度分析软件TotalLabv2 .0 1,定量分析RT PCR产物电泳带密度。结果　肿瘤中心处癌组织中CD10 5mRNA的表达水平为 0 .4 4± 0 .10 ,明显高于正常腺体组织 (P <0 .0 5 ) ;肿瘤边缘处癌组织中CD10 5mRNA的表达水平为 0 .6 0± 0 .11,明显高于中心处癌组织 (P <0 .0 5 )。CD10 5mRNA高表达与淋巴转移有关 (t=2 .71,P <0 .0 5 ) ,而与患者年龄、癌肿的大小、临床病理分期和病理类型无关 (P >0 .0 5 )。结论　乳腺癌组织中CD10 5mRNA的表达水平明显高于正常腺体组织 ,且肿瘤边缘处癌组织表达最高 ,其表达与乳腺癌的发生、发展及淋巴转移有关。